ID   MPC3_YEAST              Reviewed;         146 AA.
AC   P53311; D6VV23;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 159.
DE   RecName: Full=Mitochondrial pyruvate carrier 3 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
DE            Short=MPC3 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
DE   AltName: Full=Protein FMP43 {ECO:0000303|PubMed:14576278};
DE   Flags: Precursor;
GN   Name=MPC3 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
GN   Synonyms=FMP43 {ECO:0000303|PubMed:14576278};
GN   OrderedLocusNames=YGR243W {ECO:0000312|SGD:S000003475};
GN   ORFNames=G8620;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9090057;
RX   DOI=10.1002/(sici)1097-0061(19970315)13:3<275::aid-yea73>3.0.co;2-g;
RA   Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.;
RT   "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII
RT   reveals four open reading frames, including PFK1, the gene coding for
RT   succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with
RT   ORFs of the yeast chromosome VIII.";
RL   Yeast 13:275-280(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 76625 / YPH499;
RX   PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA   Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA   Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA   Pfanner N., Meisinger C.;
RT   "The proteome of Saccharomyces cerevisiae mitochondria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN   [8]
RP   INDUCTION.
RX   PubMed=15299026; DOI=10.1074/jbc.m403606200;
RA   Viladevall L., Serrano R., Ruiz A., Domenech G., Giraldo J., Barcelo A.,
RA   Arino J.;
RT   "Characterization of the calcium-mediated response to alkaline stress in
RT   Saccharomyces cerevisiae.";
RL   J. Biol. Chem. 279:43614-43624(2004).
RN   [9]
RP   INDUCTION.
RX   PubMed=16087739; DOI=10.1128/ec.4.8.1343-1352.2005;
RA   Proft M., Gibbons F.D., Copeland M., Roth F.P., Struhl K.;
RT   "Genomewide identification of Sko1 target promoters reveals a regulatory
RT   network that operates in response to osmotic stress in Saccharomyces
RT   cerevisiae.";
RL   Eukaryot. Cell 4:1343-1352(2005).
RN   [10]
RP   INDUCTION.
RX   PubMed=17334841; DOI=10.1007/s00284-006-0525-4;
RA   Liu X., Zhang X., Wang C., Liu L., Lei M., Bao X.;
RT   "Genetic and comparative transcriptome analysis of bromodomain factor 1 in
RT   the salt stress response of Saccharomyces cerevisiae.";
RL   Curr. Microbiol. 54:325-330(2007).
RN   [11]
RP   FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=22628554; DOI=10.1126/science.1218530;
RA   Herzig S., Raemy E., Montessuit S., Veuthey J.L., Zamboni N.,
RA   Westermann B., Kunji E.R., Martinou J.C.;
RT   "Identification and functional expression of the mitochondrial pyruvate
RT   carrier.";
RL   Science 337:93-96(2012).
RN   [12]
RP   FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX   PubMed=22628558; DOI=10.1126/science.1218099;
RA   Bricker D.K., Taylor E.B., Schell J.C., Orsak T., Boutron A., Chen Y.C.,
RA   Cox J.E., Cardon C.M., Van Vranken J.G., Dephoure N., Redin C., Boudina S.,
RA   Gygi S.P., Brivet M., Thummel C.S., Rutter J.;
RT   "A mitochondrial pyruvate carrier required for pyruvate uptake in yeast,
RT   Drosophila, and humans.";
RL   Science 337:96-100(2012).
CC   -!- FUNCTION: Mediates the uptake of pyruvate into mitochondria.
CC       {ECO:0000269|PubMed:22628554, ECO:0000269|PubMed:22628558}.
CC   -!- SUBUNIT: The functional 150 kDa pyruvate import complex is a heteromer
CC       of MPC1 and either MPC2 or MPC3. {ECO:0000269|PubMed:22628554,
CC       ECO:0000269|PubMed:22628558}.
CC   -!- INTERACTION:
CC       P53311; P53157: MPC1; NbExp=5; IntAct=EBI-23568, EBI-23845;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:14576278}. Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:22628558}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: Mainly expressed when grown on lactate containing growth
CC       medium (PubMed:22628554). Expression regulated by osmotic and alkaline
CC       stresses (PubMed:15299026, PubMed:16087739, PubMed:17334841). Under the
CC       control of the SKO1 transcription factor (PubMed:16087739).
CC       {ECO:0000269|PubMed:15299026, ECO:0000269|PubMed:16087739,
CC       ECO:0000269|PubMed:17334841, ECO:0000269|PubMed:22628554}.
CC   -!- DISRUPTION PHENOTYPE: Grows more slowly in amino acid-free medium (SD),
CC       when combined with disruption of MPC2. {ECO:0000269|PubMed:22628554}.
CC   -!- MISCELLANEOUS: Present with 6750 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the mitochondrial pyruvate carrier (MPC)
CC       (TC 2.A.105) family. {ECO:0000305}.
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DR   EMBL; Z73028; CAA97272.1; -; Genomic_DNA.
DR   EMBL; AY558546; AAS56872.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08334.1; -; Genomic_DNA.
DR   PIR; S64569; S64569.
DR   RefSeq; NP_011759.1; NM_001181372.1.
DR   AlphaFoldDB; P53311; -.
DR   BioGRID; 33494; 88.
DR   ComplexPortal; CPX-303; Mitochondrial pyruvate carrier, respiratory isoform.
DR   DIP; DIP-2027N; -.
DR   IntAct; P53311; 2.
DR   STRING; 4932.YGR243W; -.
DR   TCDB; 2.A.105.1.1; the mitochondrial pyruvate carrier (mpc) family.
DR   MaxQB; P53311; -.
DR   PaxDb; 4932-YGR243W; -.
DR   PeptideAtlas; P53311; -.
DR   EnsemblFungi; YGR243W_mRNA; YGR243W; YGR243W.
DR   GeneID; 853158; -.
DR   KEGG; sce:YGR243W; -.
DR   AGR; SGD:S000003475; -.
DR   SGD; S000003475; MPC3.
DR   VEuPathDB; FungiDB:YGR243W; -.
DR   eggNOG; KOG1589; Eukaryota.
DR   GeneTree; ENSGT00510000047120; -.
DR   HOGENOM; CLU_099502_1_0_1; -.
DR   InParanoid; P53311; -.
DR   OMA; PQQFAIC; -.
DR   OrthoDB; 590256at2759; -.
DR   BioCyc; YEAST:G3O-30919-MONOMER; -.
DR   BioGRID-ORCS; 853158; 4 hits in 10 CRISPR screens.
DR   PRO; PR:P53311; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53311; Protein.
DR   GO; GO:0098800; C:inner mitochondrial membrane protein complex; IDA:ComplexPortal.
DR   GO; GO:0031966; C:mitochondrial membrane; IDA:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0050833; F:pyruvate transmembrane transporter activity; IDA:SGD.
DR   GO; GO:0006850; P:mitochondrial pyruvate transmembrane transport; IDA:SGD.
DR   InterPro; IPR005336; MPC.
DR   PANTHER; PTHR14154:SF2; MITOCHONDRIAL PYRUVATE CARRIER 2; 1.
DR   PANTHER; PTHR14154; UPF0041 BRAIN PROTEIN 44-RELATED; 1.
DR   Pfam; PF03650; MPC; 1.
PE   1: Evidence at protein level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Stress response; Transit peptide; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..20
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..146
FT                   /note="Mitochondrial pyruvate carrier 3"
FT                   /id="PRO_0000212800"
FT   TRANSMEM        23..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   146 AA;  16230 MW;  E0B13933AB142B4E CRC64;
     MSASAFNFAF RRFWNSETGP KTVHFWAPTL KWGLVFAGLN DIKRPVEKVS GAQNLSLLAT
     ALIWTRWSFV IKPKNYLLAS VNFFLGCTAG YHLTRIANFR IRNGDSFKQV IHYIIKGETP
     AAVAAKQTAS TSMNKGVIGT NPPITH
//