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P53311 (MPC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial pyruvate carrier 3

Short name=MPC3
Alternative name(s):
Protein FMP43
Gene names
Name:FMP43
Ordered Locus Names:YGR243W
ORF Names:G8620
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length146 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the uptake of pyruvate into mitochondria. Ref.11 Ref.12

Subunit structure

The functional 150 kDa pyruvate import complex is a heteromer of mpc1 and either mpc2 or mpc3. Ref.11 Ref.12

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.5 Ref.7 Ref.11 Ref.12.

Induction

Expression regulated by osmotic and alkaline stresses. Under the control of the SKO1 transcription factor. Ref.8 Ref.9 Ref.10

Disruption phenotype

Grows more slowly in amino acid-free medium (SD), when combined with disruption of mpc2. Ref.11

Miscellaneous

Present with 6750 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the mitochondrial pyruvate carrier (MPC) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2020Mitochondrion Potential
Chain21 – 146126Mitochondrial pyruvate carrier 3
PRO_0000212800

Regions

Transmembrane23 – 3917Helical; Potential
Transmembrane55 – 7117Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P53311 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: E0B13933AB142B4E

FASTA14616,230
        10         20         30         40         50         60 
MSASAFNFAF RRFWNSETGP KTVHFWAPTL KWGLVFAGLN DIKRPVEKVS GAQNLSLLAT 

        70         80         90        100        110        120 
ALIWTRWSFV IKPKNYLLAS VNFFLGCTAG YHLTRIANFR IRNGDSFKQV IHYIIKGETP 

       130        140 
AAVAAKQTAS TSMNKGVIGT NPPITH 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[8]"Characterization of the calcium-mediated response to alkaline stress in Saccharomyces cerevisiae."
Viladevall L., Serrano R., Ruiz A., Domenech G., Giraldo J., Barcelo A., Arino J.
J. Biol. Chem. 279:43614-43624(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"Genomewide identification of Sko1 target promoters reveals a regulatory network that operates in response to osmotic stress in Saccharomyces cerevisiae."
Proft M., Gibbons F.D., Copeland M., Roth F.P., Struhl K.
Eukaryot. Cell 4:1343-1352(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[10]"Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae."
Liu X., Zhang X., Wang C., Liu L., Lei M., Bao X.
Curr. Microbiol. 54:325-330(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[11]"Identification and functional expression of the mitochondrial pyruvate carrier."
Herzig S., Raemy E., Montessuit S., Veuthey J.L., Zamboni N., Westermann B., Kunji E.R., Martinou J.C.
Science 337:93-96(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, DISRUPTION PHENOTYPE.
[12]"A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans."
Bricker D.K., Taylor E.B., Schell J.C., Orsak T., Boutron A., Chen Y.C., Cox J.E., Cardon C.M., Van Vranken J.G., Dephoure N., Redin C., Boudina S., Gygi S.P., Brivet M., Thummel C.S., Rutter J.
Science 337:96-100(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73028 Genomic DNA. Translation: CAA97272.1.
AY558546 Genomic DNA. Translation: AAS56872.1.
BK006941 Genomic DNA. Translation: DAA08334.1.
PIRS64569.
RefSeqNP_011759.1. NM_001181372.1.

3D structure databases

ProteinModelPortalP53311.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33494. 15 interactions.
DIPDIP-2027N.
MINTMINT-535950.
STRING4932.YGR243W.

Protein family/group databases

TCDB2.A.105.1.1. the mitochondrial pyruvate carrier (mpc) family.

Proteomic databases

PaxDbP53311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR243W; YGR243W; YGR243W.
GeneID853158.
KEGGsce:YGR243W.

Organism-specific databases

CYGDYGR243w.
SGDS000003475. FMP43.

Phylogenomic databases

eggNOGNOG250420.
GeneTreeENSGT00510000047120.
HOGENOMHOG000188910.
OMATINIAFR.
OrthoDBEOG72RNB3.

Enzyme and pathway databases

BioCycYEAST:G3O-30919-MONOMER.

Gene expression databases

GenevestigatorP53311.

Family and domain databases

InterProIPR005336. MPC.
[Graphical view]
PfamPF03650. MPC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973255.

Entry information

Entry nameMPC3_YEAST
AccessionPrimary (citable) accession number: P53311
Secondary accession number(s): D6VV23
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families