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Protein

Mitochondrial pyruvate carrier 3

Gene

MPC3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the uptake of pyruvate into mitochondria.2 Publications

GO - Molecular functioni

  1. pyruvate transmembrane transporter activity Source: SGD

GO - Biological processi

  1. mitochondrial pyruvate transport Source: SGD
  2. pyruvate transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Stress response, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30919-MONOMER.
ReactomeiREACT_259911. Pyruvate metabolism.

Protein family/group databases

TCDBi2.A.105.1.1. the mitochondrial pyruvate carrier (mpc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial pyruvate carrier 32 Publications
Short name:
MPC32 Publications
Alternative name(s):
Protein FMP431 Publication
Gene namesi
Name:MPC32 Publications
Synonyms:FMP431 Publication
Ordered Locus Names:YGR243WImported
ORF Names:G8620
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR243w.
SGDiS000003475. MPC3.

Subcellular locationi

Mitochondrion 2 Publications. Mitochondrion inner membrane 1 Publication; Multi-pass membrane protein Sequence Analysis

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 3917HelicalSequence AnalysisAdd
BLAST
Transmembranei55 – 7117HelicalSequence AnalysisAdd
BLAST
Transmembranei78 – 9417HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-SubCell
  3. mitochondrial membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Grows more slowly in amino acid-free medium (SD), when combined with disruption of MPC2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020MitochondrionSequence AnalysisAdd
BLAST
Chaini21 – 146126Mitochondrial pyruvate carrier 3PRO_0000212800Add
BLAST

Proteomic databases

PaxDbiP53311.

Expressioni

Inductioni

Mainly expressed when grown on lactate containing growth medium (PubMed:22628554). Expression regulated by osmotic and alkaline stresses (PubMed:15299026, PubMed:16087739, PubMed:17334841). Under the control of the SKO1 transcription factor (PubMed:16087739).4 Publications

Gene expression databases

GenevestigatoriP53311.

Interactioni

Subunit structurei

The functional 150 kDa pyruvate import complex is a heteromer of MPC1 and either MPC2 or MPC3.2 Publications

Protein-protein interaction databases

BioGridi33494. 16 interactions.
DIPiDIP-2027N.
MINTiMINT-535950.
STRINGi4932.YGR243W.

Structurei

3D structure databases

ProteinModelPortaliP53311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250420.
GeneTreeiENSGT00510000047120.
HOGENOMiHOG000188910.
InParanoidiP53311.
OMAiGPRTVHF.
OrthoDBiEOG72RNB3.

Family and domain databases

InterProiIPR005336. MPC.
[Graphical view]
PfamiPF03650. MPC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53311-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSASAFNFAF RRFWNSETGP KTVHFWAPTL KWGLVFAGLN DIKRPVEKVS
60 70 80 90 100
GAQNLSLLAT ALIWTRWSFV IKPKNYLLAS VNFFLGCTAG YHLTRIANFR
110 120 130 140
IRNGDSFKQV IHYIIKGETP AAVAAKQTAS TSMNKGVIGT NPPITH
Length:146
Mass (Da):16,230
Last modified:October 1, 1996 - v1
Checksum:iE0B13933AB142B4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73028 Genomic DNA. Translation: CAA97272.1.
AY558546 Genomic DNA. Translation: AAS56872.1.
BK006941 Genomic DNA. Translation: DAA08334.1.
PIRiS64569.
RefSeqiNP_011759.1. NM_001181372.1.

Genome annotation databases

EnsemblFungiiYGR243W; YGR243W; YGR243W.
GeneIDi853158.
KEGGisce:YGR243W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73028 Genomic DNA. Translation: CAA97272.1.
AY558546 Genomic DNA. Translation: AAS56872.1.
BK006941 Genomic DNA. Translation: DAA08334.1.
PIRiS64569.
RefSeqiNP_011759.1. NM_001181372.1.

3D structure databases

ProteinModelPortaliP53311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33494. 16 interactions.
DIPiDIP-2027N.
MINTiMINT-535950.
STRINGi4932.YGR243W.

Protein family/group databases

TCDBi2.A.105.1.1. the mitochondrial pyruvate carrier (mpc) family.

Proteomic databases

PaxDbiP53311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR243W; YGR243W; YGR243W.
GeneIDi853158.
KEGGisce:YGR243W.

Organism-specific databases

CYGDiYGR243w.
SGDiS000003475. MPC3.

Phylogenomic databases

eggNOGiNOG250420.
GeneTreeiENSGT00510000047120.
HOGENOMiHOG000188910.
InParanoidiP53311.
OMAiGPRTVHF.
OrthoDBiEOG72RNB3.

Enzyme and pathway databases

BioCyciYEAST:G3O-30919-MONOMER.
ReactomeiREACT_259911. Pyruvate metabolism.

Miscellaneous databases

NextBioi973255.

Gene expression databases

GenevestigatoriP53311.

Family and domain databases

InterProiIPR005336. MPC.
[Graphical view]
PfamiPF03650. MPC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
    Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
    Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "Characterization of the calcium-mediated response to alkaline stress in Saccharomyces cerevisiae."
    Viladevall L., Serrano R., Ruiz A., Domenech G., Giraldo J., Barcelo A., Arino J.
    J. Biol. Chem. 279:43614-43624(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "Genomewide identification of Sko1 target promoters reveals a regulatory network that operates in response to osmotic stress in Saccharomyces cerevisiae."
    Proft M., Gibbons F.D., Copeland M., Roth F.P., Struhl K.
    Eukaryot. Cell 4:1343-1352(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae."
    Liu X., Zhang X., Wang C., Liu L., Lei M., Bao X.
    Curr. Microbiol. 54:325-330(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "Identification and functional expression of the mitochondrial pyruvate carrier."
    Herzig S., Raemy E., Montessuit S., Veuthey J.L., Zamboni N., Westermann B., Kunji E.R., Martinou J.C.
    Science 337:93-96(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, INDUCTION.
  12. Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.

Entry informationi

Entry nameiMPC3_YEAST
AccessioniPrimary (citable) accession number: P53311
Secondary accession number(s): D6VV23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.