ID   AP18B_YEAST             Reviewed;         568 AA.
AC   P53309;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 66.
DE   RecName: Full=Clathrin coat assembly protein AP180B;
GN   Name=YAP1802; OrderedLocusNames=YGR241C; ORFNames=G8610;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=97245298; PubMed=9090057;
RX   DOI=10.1002/(SICI)1097-0061(19970315)13:3<275::AID-YEA73>3.3.CO;2-7;
RA   Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.;
RT   "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome
RT   VII reveals four open reading frames, including PFK1, the gene coding
RT   for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology
RT   with ORFs of the yeast chromosome VIII.";
RL   Yeast 13:275-280(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=97313265; PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M.,
RA   Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J.,
RA   Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E.,
RA   Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E.,
RA   Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B.,
RA   Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L.,
RA   Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M.,
RA   Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M.,
RA   Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B.,
RA   Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W.,
RA   Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A.,
RA   Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S.,
RA   Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L.,
RA   Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S.,
RA   Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J.,
RA   Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M.,
RA   Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B.,
RA   Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J.,
RA   Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M.,
RA   van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M.,
RA   Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H.,
RA   Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M.,
RA   Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH CHC1; CLC1 AND PAN1.
RX   PubMed=9531549; DOI=10.1083/jcb.141.1.71;
RA   Wendland B., Emr S.D.;
RT   "Pan1p, yeast eps15, functions as a multivalent adaptor that
RT   coordinates protein-protein interactions essential for endocytosis.";
RL   J. Cell Biol. 141:71-84(1998).
RN   [4]
RP   FUNCTION.
RX   PubMed=14704157;
RA   Baggett J.J., D'Aquino K.E., Wendland B.;
RT   "The Sla2p talin domain plays a role in endocytosis in Saccharomyces
RT   cerevisiae.";
RL   Genetics 165:1661-1674(2003).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Involved in endocytosis and clathrin cage assembly.
CC   -!- SUBUNIT: Interacts with PAN1 and the clathrin heavy and light
CC       chains CHC1 and CLC1.
CC   -!- INTERACTION:
CC       P38800:-; NbExp=1; IntAct=EBI-23557, EBI-24597;
CC       P53974:ARK1; NbExp=1; IntAct=EBI-23557, EBI-9817;
CC       P32660:DNF1; NbExp=1; IntAct=EBI-23557, EBI-3121;
CC       P25623:SYP1; NbExp=1; IntAct=EBI-23557, EBI-21900;
CC   -!- SUBCELLULAR LOCATION: Bud. Bud neck. Cell membrane; Peripheral
CC       membrane protein; Cytoplasmic side. Cytoplasm.
CC   -!- MISCELLANEOUS: Present with 6800 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the AP180 family.
CC   -!- SIMILARITY: Contains 1 ENTH (epsin N-terminal homology) domain.
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DR   EMBL; Z73026; CAA97270.1; -; Genomic_DNA.
DR   PIR; S64567; S64567.
DR   RefSeq; NP_011757.1; -.
DR   HSSP; O96528; 1HX8.
DR   DIP; DIP:2751N; -.
DR   IntAct; P53309; 16.
DR   Ensembl; YGR241C; Saccharomyces cerevisiae.
DR   GeneID; 853157; -.
DR   GenomeReviews; Y13135_GR; YGR241C.
DR   KEGG; sce:YGR241C; -.
DR   NMPDR; fig|4932.3.peg.2886; -.
DR   CYGD; YGR241c; -.
DR   SGD; S000003473; YAP1802.
DR   HOGENOM; P53309; -.
DR   OMA; P53309; IHIMIRE.
DR   NextBio; 973252; -.
DR   ArrayExpress; P53309; -.
DR   GermOnline; YGR241C; Saccharomyces cerevisiae.
DR   GO; GO:0030479; C:actin cortical patch; TAS:SGD.
DR   GO; GO:0005935; C:cellular bud neck; IEA:UniProtKB-SubCell.
DR   GO; GO:0030118; C:clathrin coat; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030276; F:clathrin binding; TAS:SGD.
DR   GO; GO:0005545; F:phosphatidylinositol binding; IEA:InterPro.
DR   GO; GO:0048268; P:clathrin coat assembly; IEA:InterPro.
DR   GO; GO:0006897; P:endocytosis; IPI:SGD.
DR   InterPro; IPR011417; ANTH.
DR   InterPro; IPR014712; Clathrin_Pinositid-bd_GAT-like.
DR   InterPro; IPR008942; ENTH_VHS.
DR   InterPro; IPR013809; Epsin-like_N.
DR   Gene3D; G3DSA:1.25.40.90; ENTH_VHS; 1.
DR   Gene3D; G3DSA:1.20.58.150; Pinositid-bd_clathrin_GAT-like; 1.
DR   Pfam; PF07651; ANTH; 1.
DR   SMART; SM00273; ENTH; 1.
DR   PROSITE; PS50942; ENTH; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Complete proteome; Cytoplasm; Endocytosis; Membrane;
KW   Phosphoprotein.
FT   CHAIN         1    568       Clathrin coat assembly protein AP180B.
FT                                /FTId=PRO_0000202856.
FT   DOMAIN        1    127       ENTH.
FT   COMPBIAS    379    383       Poly-Gln.
FT   COMPBIAS    464    467       Poly-Gln.
FT   COMPBIAS    551    560       Poly-Gln.
FT   MOD_RES     133    133       Phosphoserine.
SQ   SEQUENCE   568 AA;  64328 MW;  C5B5FD733739C3CD CRC64;
     MSSLYTKLVK GATKIKMAPP KQKYVDPILS GTSSARGLQE ITHALDIRLS DTAWTIVYKA
     LIVLHLMIQQ GEKDVTLRHY SHNLDVFQLR KISHTTKWSS NDMRALQRYD EYLKTRCEEY
     GRLGMDHLRD NYSSLKLGSK NQLSMDEELD HVESLEIQIN ALIRNKYSVS DLENHLLLYA
     FQLLVQDLLG LYNALNEGVI TLLESFFELS IEHAKRTLDL YKDFVDMTEY VVRYLKIGKA
     VGLKIPVIKH ITTKLINSLE EHLREETKRQ RGEPSEPQQD RKPSTAISST SSHNNNSNDK
     NKSIAQKKLE QIREQKRLLE QQLQNQQLLI SPTVPQDAYN PFGSQQQDLN NDTFSFEPTQ
     PQMTAQVPQP TANPFLIPQQ QQQALQLTSA STMPQPSEIQ ITPNLNNQQT GMYASNLQYT
     PNFTGSGFGG YTTTENNAIM TGTLDPTKTG SNNPFSLENI AREQQQQNFQ NSPNPFTLQQ
     AQTTPILAHS QTGNPFQAQN VVTSPMGTYM TNPVAGQLQY ASTGAQQQPQ MMQGQQTGYV
     MVPTAFVPIN QQQQQQQHQQ ENPNLIDI
//