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Protein

Clathrin coat assembly protein AP180B

Gene

YAP1802

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endocytosis and clathrin cage assembly.2 Publications

GO - Molecular functioni

  1. 1-phosphatidylinositol binding Source: InterPro
  2. clathrin binding Source: SGD

GO - Biological processi

  1. clathrin coat assembly Source: InterPro
  2. endocytosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30918-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin coat assembly protein AP180B
Gene namesi
Name:YAP1802
Ordered Locus Names:YGR241C
ORF Names:G8610
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR241c.
SGDiS000003473. YAP1802.

Subcellular locationi

Bud 1 Publication. Bud neck 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cytoplasm 1 Publication

GO - Cellular componenti

  1. actin cortical patch Source: SGD
  2. cellular bud neck Source: UniProtKB-SubCell
  3. clathrin-coated vesicle Source: InterPro
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 568568Clathrin coat assembly protein AP180BPRO_0000202856Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei449 – 4491Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53309.
PaxDbiP53309.

Expressioni

Gene expression databases

GenevestigatoriP53309.

Interactioni

Subunit structurei

Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1.1 Publication

Protein-protein interaction databases

BioGridi33493. 48 interactions.
DIPiDIP-2751N.
IntActiP53309. 17 interactions.
MINTiMINT-556894.
STRINGi4932.YGR241C.

Structurei

3D structure databases

ProteinModelPortaliP53309.
SMRiP53309. Positions 3-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 127127ENTHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi379 – 3835Poly-Gln
Compositional biasi464 – 4674Poly-Gln
Compositional biasi551 – 56010Poly-Gln

Sequence similaritiesi

Belongs to the AP180 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG267212.
GeneTreeiENSGT00390000008805.
HOGENOMiHOG000033975.
InParanoidiP53309.
OMAiREVESVQ.
OrthoDBiEOG7Q5HNZ.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLYTKLVK GATKIKMAPP KQKYVDPILS GTSSARGLQE ITHALDIRLS
60 70 80 90 100
DTAWTIVYKA LIVLHLMIQQ GEKDVTLRHY SHNLDVFQLR KISHTTKWSS
110 120 130 140 150
NDMRALQRYD EYLKTRCEEY GRLGMDHLRD NYSSLKLGSK NQLSMDEELD
160 170 180 190 200
HVESLEIQIN ALIRNKYSVS DLENHLLLYA FQLLVQDLLG LYNALNEGVI
210 220 230 240 250
TLLESFFELS IEHAKRTLDL YKDFVDMTEY VVRYLKIGKA VGLKIPVIKH
260 270 280 290 300
ITTKLINSLE EHLREETKRQ RGEPSEPQQD RKPSTAISST SSHNNNSNDK
310 320 330 340 350
NKSIAQKKLE QIREQKRLLE QQLQNQQLLI SPTVPQDAYN PFGSQQQDLN
360 370 380 390 400
NDTFSFEPTQ PQMTAQVPQP TANPFLIPQQ QQQALQLTSA STMPQPSEIQ
410 420 430 440 450
ITPNLNNQQT GMYASNLQYT PNFTGSGFGG YTTTENNAIM TGTLDPTKTG
460 470 480 490 500
SNNPFSLENI AREQQQQNFQ NSPNPFTLQQ AQTTPILAHS QTGNPFQAQN
510 520 530 540 550
VVTSPMGTYM TNPVAGQLQY ASTGAQQQPQ MMQGQQTGYV MVPTAFVPIN
560
QQQQQQQHQQ ENPNLIDI
Length:568
Mass (Da):64,328
Last modified:October 1, 1996 - v1
Checksum:iC5B5FD733739C3CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73026 Genomic DNA. Translation: CAA97270.1.
BK006941 Genomic DNA. Translation: DAA08333.1.
PIRiS64567.
RefSeqiNP_011757.3. NM_001181370.3.

Genome annotation databases

EnsemblFungiiYGR241C; YGR241C; YGR241C.
GeneIDi853157.
KEGGisce:YGR241C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73026 Genomic DNA. Translation: CAA97270.1.
BK006941 Genomic DNA. Translation: DAA08333.1.
PIRiS64567.
RefSeqiNP_011757.3. NM_001181370.3.

3D structure databases

ProteinModelPortaliP53309.
SMRiP53309. Positions 3-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33493. 48 interactions.
DIPiDIP-2751N.
IntActiP53309. 17 interactions.
MINTiMINT-556894.
STRINGi4932.YGR241C.

Proteomic databases

MaxQBiP53309.
PaxDbiP53309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR241C; YGR241C; YGR241C.
GeneIDi853157.
KEGGisce:YGR241C.

Organism-specific databases

CYGDiYGR241c.
SGDiS000003473. YAP1802.

Phylogenomic databases

eggNOGiNOG267212.
GeneTreeiENSGT00390000008805.
HOGENOMiHOG000033975.
InParanoidiP53309.
OMAiREVESVQ.
OrthoDBiEOG7Q5HNZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30918-MONOMER.

Miscellaneous databases

NextBioi973252.

Gene expression databases

GenevestigatoriP53309.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
    Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
    Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis."
    Wendland B., Emr S.D.
    J. Cell Biol. 141:71-84(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CHC1; CLC1 AND PAN1.
  5. "The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae."
    Baggett J.J., D'Aquino K.E., Wendland B.
    Genetics 165:1661-1674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-449, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP18B_YEAST
AccessioniPrimary (citable) accession number: P53309
Secondary accession number(s): D6VV22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.