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P53309 (AP18B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Clathrin coat assembly protein AP180B
Gene names
Name:YAP1802
Ordered Locus Names:YGR241C
ORF Names:G8610
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in endocytosis and clathrin cage assembly. Ref.4 Ref.5

Subunit structure

Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1. Ref.4

Subcellular location

Bud. Bud neck. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm Ref.6.

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AP180 family.

Contains 1 ENTH (epsin N-terminal homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568Clathrin coat assembly protein AP180B
PRO_0000202856

Regions

Domain1 – 127127ENTH
Compositional bias379 – 3835Poly-Gln
Compositional bias464 – 4674Poly-Gln
Compositional bias551 – 56010Poly-Gln

Amino acid modifications

Modified residue4491Phosphothreonine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P53309 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: C5B5FD733739C3CD

FASTA56864,328
        10         20         30         40         50         60 
MSSLYTKLVK GATKIKMAPP KQKYVDPILS GTSSARGLQE ITHALDIRLS DTAWTIVYKA 

        70         80         90        100        110        120 
LIVLHLMIQQ GEKDVTLRHY SHNLDVFQLR KISHTTKWSS NDMRALQRYD EYLKTRCEEY 

       130        140        150        160        170        180 
GRLGMDHLRD NYSSLKLGSK NQLSMDEELD HVESLEIQIN ALIRNKYSVS DLENHLLLYA 

       190        200        210        220        230        240 
FQLLVQDLLG LYNALNEGVI TLLESFFELS IEHAKRTLDL YKDFVDMTEY VVRYLKIGKA 

       250        260        270        280        290        300 
VGLKIPVIKH ITTKLINSLE EHLREETKRQ RGEPSEPQQD RKPSTAISST SSHNNNSNDK 

       310        320        330        340        350        360 
NKSIAQKKLE QIREQKRLLE QQLQNQQLLI SPTVPQDAYN PFGSQQQDLN NDTFSFEPTQ 

       370        380        390        400        410        420 
PQMTAQVPQP TANPFLIPQQ QQQALQLTSA STMPQPSEIQ ITPNLNNQQT GMYASNLQYT 

       430        440        450        460        470        480 
PNFTGSGFGG YTTTENNAIM TGTLDPTKTG SNNPFSLENI AREQQQQNFQ NSPNPFTLQQ 

       490        500        510        520        530        540 
AQTTPILAHS QTGNPFQAQN VVTSPMGTYM TNPVAGQLQY ASTGAQQQPQ MMQGQQTGYV 

       550        560 
MVPTAFVPIN QQQQQQQHQQ ENPNLIDI 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis."
Wendland B., Emr S.D.
J. Cell Biol. 141:71-84(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CHC1; CLC1 AND PAN1.
[5]"The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae."
Baggett J.J., D'Aquino K.E., Wendland B.
Genetics 165:1661-1674(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-449, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73026 Genomic DNA. Translation: CAA97270.1.
BK006941 Genomic DNA. Translation: DAA08333.1.
PIRS64567.
RefSeqNP_011757.3. NM_001181370.3.

3D structure databases

ProteinModelPortalP53309.
SMRP53309. Positions 3-263.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33493. 48 interactions.
DIPDIP-2751N.
IntActP53309. 17 interactions.
MINTMINT-556894.
STRING4932.YGR241C.

Proteomic databases

PaxDbP53309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR241C; YGR241C; YGR241C.
GeneID853157.
KEGGsce:YGR241C.

Organism-specific databases

CYGDYGR241c.
SGDS000003473. YAP1802.

Phylogenomic databases

eggNOGNOG267212.
GeneTreeENSGT00390000008805.
HOGENOMHOG000033975.
OMAQEITHAL.
OrthoDBEOG7Q5HNZ.

Enzyme and pathway databases

BioCycYEAST:G3O-30918-MONOMER.

Gene expression databases

GenevestigatorP53309.

Family and domain databases

Gene3D1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProIPR011417. ANTH_dom.
IPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
[Graphical view]
PfamPF07651. ANTH. 1 hit.
[Graphical view]
SMARTSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMSSF48464. SSF48464. 1 hit.
PROSITEPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973252.

Entry information

Entry nameAP18B_YEAST
AccessionPrimary (citable) accession number: P53309
Secondary accession number(s): D6VV22
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families