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P53309

- AP18B_YEAST

UniProt

P53309 - AP18B_YEAST

Protein

Clathrin coat assembly protein AP180B

Gene

YAP1802

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in endocytosis and clathrin cage assembly.2 Publications

    GO - Molecular functioni

    1. 1-phosphatidylinositol binding Source: InterPro
    2. clathrin binding Source: SGD

    GO - Biological processi

    1. clathrin coat assembly Source: InterPro
    2. endocytosis Source: SGD

    Keywords - Biological processi

    Endocytosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30918-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Clathrin coat assembly protein AP180B
    Gene namesi
    Name:YAP1802
    Ordered Locus Names:YGR241C
    ORF Names:G8610
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR241c.
    SGDiS000003473. YAP1802.

    Subcellular locationi

    Bud 1 Publication. Bud neck 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. actin cortical patch Source: SGD
    2. cellular bud neck Source: UniProtKB-SubCell
    3. clathrin coat Source: InterPro
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568Clathrin coat assembly protein AP180BPRO_0000202856Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei449 – 4491Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53309.
    PaxDbiP53309.

    Expressioni

    Gene expression databases

    GenevestigatoriP53309.

    Interactioni

    Subunit structurei

    Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1.1 Publication

    Protein-protein interaction databases

    BioGridi33493. 48 interactions.
    DIPiDIP-2751N.
    IntActiP53309. 17 interactions.
    MINTiMINT-556894.
    STRINGi4932.YGR241C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53309.
    SMRiP53309. Positions 3-263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 127127ENTHPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi379 – 3835Poly-Gln
    Compositional biasi464 – 4674Poly-Gln
    Compositional biasi551 – 56010Poly-Gln

    Sequence similaritiesi

    Belongs to the AP180 family.Curated
    Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG267212.
    GeneTreeiENSGT00390000008805.
    HOGENOMiHOG000033975.
    OMAiAPKPKYI.
    OrthoDBiEOG7Q5HNZ.

    Family and domain databases

    Gene3Di1.20.58.150. 1 hit.
    1.25.40.90. 1 hit.
    InterProiIPR011417. ANTH_dom.
    IPR014712. Clathrin_Pinositid-bd_GAT-like.
    IPR008942. ENTH_VHS.
    IPR013809. Epsin-like_N.
    [Graphical view]
    PfamiPF07651. ANTH. 1 hit.
    [Graphical view]
    SMARTiSM00273. ENTH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48464. SSF48464. 1 hit.
    PROSITEiPS50942. ENTH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53309-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLYTKLVK GATKIKMAPP KQKYVDPILS GTSSARGLQE ITHALDIRLS    50
    DTAWTIVYKA LIVLHLMIQQ GEKDVTLRHY SHNLDVFQLR KISHTTKWSS 100
    NDMRALQRYD EYLKTRCEEY GRLGMDHLRD NYSSLKLGSK NQLSMDEELD 150
    HVESLEIQIN ALIRNKYSVS DLENHLLLYA FQLLVQDLLG LYNALNEGVI 200
    TLLESFFELS IEHAKRTLDL YKDFVDMTEY VVRYLKIGKA VGLKIPVIKH 250
    ITTKLINSLE EHLREETKRQ RGEPSEPQQD RKPSTAISST SSHNNNSNDK 300
    NKSIAQKKLE QIREQKRLLE QQLQNQQLLI SPTVPQDAYN PFGSQQQDLN 350
    NDTFSFEPTQ PQMTAQVPQP TANPFLIPQQ QQQALQLTSA STMPQPSEIQ 400
    ITPNLNNQQT GMYASNLQYT PNFTGSGFGG YTTTENNAIM TGTLDPTKTG 450
    SNNPFSLENI AREQQQQNFQ NSPNPFTLQQ AQTTPILAHS QTGNPFQAQN 500
    VVTSPMGTYM TNPVAGQLQY ASTGAQQQPQ MMQGQQTGYV MVPTAFVPIN 550
    QQQQQQQHQQ ENPNLIDI 568
    Length:568
    Mass (Da):64,328
    Last modified:October 1, 1996 - v1
    Checksum:iC5B5FD733739C3CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73026 Genomic DNA. Translation: CAA97270.1.
    BK006941 Genomic DNA. Translation: DAA08333.1.
    PIRiS64567.
    RefSeqiNP_011757.3. NM_001181370.3.

    Genome annotation databases

    EnsemblFungiiYGR241C; YGR241C; YGR241C.
    GeneIDi853157.
    KEGGisce:YGR241C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73026 Genomic DNA. Translation: CAA97270.1 .
    BK006941 Genomic DNA. Translation: DAA08333.1 .
    PIRi S64567.
    RefSeqi NP_011757.3. NM_001181370.3.

    3D structure databases

    ProteinModelPortali P53309.
    SMRi P53309. Positions 3-263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33493. 48 interactions.
    DIPi DIP-2751N.
    IntActi P53309. 17 interactions.
    MINTi MINT-556894.
    STRINGi 4932.YGR241C.

    Proteomic databases

    MaxQBi P53309.
    PaxDbi P53309.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR241C ; YGR241C ; YGR241C .
    GeneIDi 853157.
    KEGGi sce:YGR241C.

    Organism-specific databases

    CYGDi YGR241c.
    SGDi S000003473. YAP1802.

    Phylogenomic databases

    eggNOGi NOG267212.
    GeneTreei ENSGT00390000008805.
    HOGENOMi HOG000033975.
    OMAi APKPKYI.
    OrthoDBi EOG7Q5HNZ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30918-MONOMER.

    Miscellaneous databases

    NextBioi 973252.

    Gene expression databases

    Genevestigatori P53309.

    Family and domain databases

    Gene3Di 1.20.58.150. 1 hit.
    1.25.40.90. 1 hit.
    InterProi IPR011417. ANTH_dom.
    IPR014712. Clathrin_Pinositid-bd_GAT-like.
    IPR008942. ENTH_VHS.
    IPR013809. Epsin-like_N.
    [Graphical view ]
    Pfami PF07651. ANTH. 1 hit.
    [Graphical view ]
    SMARTi SM00273. ENTH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48464. SSF48464. 1 hit.
    PROSITEi PS50942. ENTH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII."
      Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C.
      Yeast 13:275-280(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis."
      Wendland B., Emr S.D.
      J. Cell Biol. 141:71-84(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CHC1; CLC1 AND PAN1.
    5. "The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae."
      Baggett J.J., D'Aquino K.E., Wendland B.
      Genetics 165:1661-1674(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-449, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP18B_YEAST
    AccessioniPrimary (citable) accession number: P53309
    Secondary accession number(s): D6VV22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3