Reviewed, UniProtKB/Swiss-Prot P53309 (AP18B_YEAST)

Names and origin

Protein names

Recommended name:
    Clathrin coat assembly protein AP180B

Gene names

Name:	YAP1802
Ordered Locus Names:	YGR241C
ORF Names:	G8610

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in endocytosis and clathrin cage assembly. Ref.3 Ref.4
Subunit structure	Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1. Ref.3
Subcellular location	Bud. Bud neck. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Ref.5
Miscellaneous	Present with 6800 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the AP180 family. Contains 1 ENTH (epsin N-terminal homology) domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Cytoplasm Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	clathrin coat assembly Inferred from electronic annotation. Source: InterPro endocytosis Ref.3 Inferred from physical interaction. Source: SGD
Cellular component	actin cortical patch Traceable author statement. Source: SGD cellular bud neck Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin coat Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	clathrin binding Traceable author statement. Source: SGD phosphatidylinositol binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-23557,EBI-24597
ARK1	P53974	1	EBI-23557,EBI-9817
DNF1	P32660	1	EBI-23557,EBI-3121
SYP1	P25623	1	EBI-23557,EBI-21900

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Clathrin coat assembly protein AP180B		PRO_0000202856
Regions
	Domain	1 – 127	127	ENTH
	Compositional bias	379 – 383	5	Poly-Gln
	Compositional bias	464 – 467	4	Poly-Gln
	Compositional bias	551 – 560	10	Poly-Gln
Amino acid modifications
	Modified residue	133	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P53309-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C5B5FD733739C3CD	FASTA	568	64,328
	10 20 30 40 50 60 MSSLYTKLVK GATKIKMAPP KQKYVDPILS GTSSARGLQE ITHALDIRLS DTAWTIVYKA 70 80 90 100 110 120 LIVLHLMIQQ GEKDVTLRHY SHNLDVFQLR KISHTTKWSS NDMRALQRYD EYLKTRCEEY 130 140 150 160 170 180 GRLGMDHLRD NYSSLKLGSK NQLSMDEELD HVESLEIQIN ALIRNKYSVS DLENHLLLYA 190 200 210 220 230 240 FQLLVQDLLG LYNALNEGVI TLLESFFELS IEHAKRTLDL YKDFVDMTEY VVRYLKIGKA 250 260 270 280 290 300 VGLKIPVIKH ITTKLINSLE EHLREETKRQ RGEPSEPQQD RKPSTAISST SSHNNNSNDK 310 320 330 340 350 360 NKSIAQKKLE QIREQKRLLE QQLQNQQLLI SPTVPQDAYN PFGSQQQDLN NDTFSFEPTQ 370 380 390 400 410 420 PQMTAQVPQP TANPFLIPQQ QQQALQLTSA STMPQPSEIQ ITPNLNNQQT GMYASNLQYT 430 440 450 460 470 480 PNFTGSGFGG YTTTENNAIM TGTLDPTKTG SNNPFSLENI AREQQQQNFQ NSPNPFTLQQ 490 500 510 520 530 540 AQTTPILAHS QTGNPFQAQN VVTSPMGTYM TNPVAGQLQY ASTGAQQQPQ MMQGQQTGYV 550 560 MVPTAFVPIN QQQQQQQHQQ ENPNLIDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII." Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C. Yeast 13:275-280(1997) [PubMed: 9090057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis." Wendland B., Emr S.D. J. Cell Biol. 141:71-84(1998) [PubMed: 9531549] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHC1; CLC1 AND PAN1.
[4]	"The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae." Baggett J.J., D'Aquino K.E., Wendland B. Genetics 165:1661-1674(2003) [PubMed: 14704157] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73026 Genomic DNA. Translation: CAA97270.1.
PIR	S64567.
RefSeq	NP_011757.1.
3D structure databases
HSSP	HSSP built from PDB template 1HX8 based on UniProtKB O96528.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2751N.
IntAct	P53309. 16 interactions.
Genome annotation databases
Ensembl	YGR241C. Saccharomyces cerevisiae. [Contig view]
GeneID	853157.
GenomeReviews	Gene locus YGR241C in contig Y13135_GR.
KEGG	sce:YGR241C.
NMPDR	fig|4932.3.peg.2886.
Organism-specific databases
CYGD	YGR241c.
SGD	S000003473. YAP1802.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53309.
OMA	P53309. IHIMIRE.
Gene expression databases
ArrayExpress	P53309.
GermOnline	YGR241C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011417. ANTH. IPR014712. Clathrin_Pinositid-bd_GAT-like. IPR008942. ENTH_VHS. IPR013809. Epsin-like_N. [Graphical view]
Gene3D	G3DSA:1.25.40.90. ENTH_VHS. 1 hit. G3DSA:1.20.58.150. Pinositid-bd_clathrin_GAT-like. 1 hit.
Pfam	PF07651. ANTH. 1 hit. [Graphical view]
SMART	SM00273. ENTH. 1 hit. [Graphical view]
PROSITE	PS50942. ENTH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973252.

Entry information

Entry name

AP18B_YEAST

Accession

Primary (citable) accession number: P53309

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)