Reviewed,
UniProtKB/Swiss-Prot P53309 (AP18B_YEAST)
Last modified
June 16, 2009.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Clathrin coat assembly protein AP180B | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 568 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in endocytosis and clathrin cage assembly. Ref.3 Ref.4 |
| Subunit structure | Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1. Ref.3 |
| Subcellular location | Bud. Bud neck. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Ref.5 |
| Miscellaneous | Present with 6800 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the AP180 family. Contains 1 ENTH (epsin N-terminal homology) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Endocytosis |
| Cellular component | Cell membrane Cytoplasm Membrane |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | clathrin coat assembly Inferred from electronic annotation. Source: InterPro endocytosis Ref.3Inferred from physical interaction. Source: SGD |
| Cellular component | actin cortical patch Traceable author statement. Source: SGD cellular bud neckInferred from electronic annotation. Source: UniProtKB-SubCell clathrin coatInferred from electronic annotation. Source: InterPro plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | clathrin binding Traceable author statement. Source: SGD phosphatidylinositol bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| P38800 | 1 | EBI-23557,EBI-24597 | ||
| ARK1 | P53974 | 1 | EBI-23557,EBI-9817 | |
| DNF1 | P32660 | 1 | EBI-23557,EBI-3121 | |
| SYP1 | P25623 | 1 | EBI-23557,EBI-21900 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 568 | 568 | Clathrin coat assembly protein AP180B | PRO_0000202856 | |||||
Regions | |||||||||
| Domain | 1 – 127 | 127 | ENTH | ||||||
| Compositional bias | 379 – 383 | 5 | Poly-Gln | ||||||
| Compositional bias | 464 – 467 | 4 | Poly-Gln | ||||||
| Compositional bias | 551 – 560 | 10 | Poly-Gln | ||||||
Amino acid modifications | |||||||||
| Modified residue | 133 | 1 | Phosphoserine Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII." Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C. Yeast 13:275-280(1997) [PubMed: 9090057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis." Wendland B., Emr S.D. J. Cell Biol. 141:71-84(1998) [PubMed: 9531549] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHC1; CLC1 AND PAN1. |
| [4] | "The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae." Baggett J.J., D'Aquino K.E., Wendland B. Genetics 165:1661-1674(2003) [PubMed: 14704157] [Abstract] Cited for: FUNCTION. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z73026 Genomic DNA. Translation: CAA97270.1. | |
| PIR | S64567. |
| RefSeq | NP_011757.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HX8 based on UniProtKB O96528. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2751N. |
| IntAct | P53309. 16 interactions. |
Genome annotation databases | |
| Ensembl | YGR241C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 853157. |
| GenomeReviews | Gene locus YGR241C in contig Y13135_GR. |
| KEGG | sce:YGR241C. |
| NMPDR | fig|4932.3.peg.2886. |
Organism-specific databases | |
| CYGD | YGR241c. |
| SGD | S000003473. YAP1802. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P53309. |
| OMA | P53309. IHIMIRE. |
Gene expression databases | |
| ArrayExpress | P53309. |
| GermOnline | YGR241C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR011417. ANTH. IPR014712. Clathrin_Pinositid-bd_GAT-like. IPR008942. ENTH_VHS. IPR013809. Epsin-like_N. [Graphical view] |
| Gene3D | G3DSA:1.25.40.90. ENTH_VHS. 1 hit. G3DSA:1.20.58.150. Pinositid-bd_clathrin_GAT-like. 1 hit. |
| Pfam | PF07651. ANTH. 1 hit. [Graphical view] |
| SMART | SM00273. ENTH. 1 hit. [Graphical view] |
| PROSITE | PS50942. ENTH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 973252. |
Entry information
| Entry name | AP18B_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53309 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |

Clusters with


