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Protein

Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1

Gene

GPI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1-phosphatidyl-1D-myo-inositol = UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol.

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-30898-MONOMER.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1 (EC:2.4.1.198)
Gene namesi
Name:GPI1
Ordered Locus Names:YGR216C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR216C.
SGDiS000003448. GPI1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 186ExtracellularSequence analysisAdd BLAST186
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Topological domaini208 – 280CytoplasmicSequence analysisAdd BLAST73
Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
Topological domaini302 – 380ExtracellularSequence analysisAdd BLAST79
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 451CytoplasmicSequence analysisAdd BLAST50
Transmembranei452 – 472HelicalSequence analysisAdd BLAST21
Topological domaini473 – 485ExtracellularSequence analysisAdd BLAST13
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Topological domaini507 – 609CytoplasmicSequence analysisAdd BLAST103

GO - Cellular componenti

  • glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Source: SGD
  • integral component of membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002156661 – 609Phosphatidylinositol N-acetylglucosaminyltransferase subunit GPI1Add BLAST609

Proteomic databases

MaxQBiP53306.
PRIDEiP53306.

Interactioni

Subunit structurei

Component of a complex with GPI1, GPI2, GPI3, GPI15 and ERI1.Curated

Protein-protein interaction databases

BioGridi33469. 74 interactors.
DIPiDIP-7773N.
IntActiP53306. 2 interactors.
MINTiMINT-1365234.

Structurei

3D structure databases

ProteinModelPortaliP53306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGQ family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004994.
HOGENOMiHOG000000940.
InParanoidiP53306.
KOiK03860.
OMAiALINHFP.
OrthoDBiEOG092C5W2N.

Family and domain databases

InterProiIPR007720. GlcNAc_Gpi1.
[Graphical view]
PfamiPF05024. Gpi1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNYIFWPYE SLFENSAAQG PQVALAISFE KTHFVVLGVC EPQYLEEVSI
60 70 80 90 100
RPPYSVVATK NNGAEGWNYK VADPCNVHFR IPKLKFMQFY SSDPISLIIP
110 120 130 140 150
EKEVGLHSSV GETLNYSKLE QHPRYKRDNK KLSETLNIIN LFPAYCKALN
160 170 180 190 200
ELYPFIQTSQ ENLRGTMLNS VAAWCSSTCI YKMVAKIGFY LTFVICSIAS
210 220 230 240 250
LVSSLLNYSH FQLVNYSAFV QQIDLRCQQI CYFPVQYERI NKKDNIQNVG
260 270 280 290 300
SMVEKDNSNS QFSHSYMPSK FYPDYILLYN TIWLIINDIS FGLILGAILI
310 320 330 340 350
ENRDFLVSAS HRVLKFFLYD SLKTITETLA NNPLGIKLNA ELANFLSELF
360 370 380 390 400
LWVIEFSYTT FIKRLIDPKT LSSLLTLTIY MMFLVGFSFA VSLAIDFFAI
410 420 430 440 450
LSFPIYVFYR ISSKLYHCQL NIMASLFNLF CGKKRNVLRN RIDHNYFQLD
460 470 480 490 500
QLLLGTLLFI ILVFLTPTVM AFYMSYTVLR MLTITIEIFS EAVIALINHF
510 520 530 540 550
PLFALLLRLK DPKRLPGGIS IELKTTVSNK HTTLELQNNP IKFKSMFRPY
560 570 580 590 600
NLLLSQMRTN YFSFATVRKI VRGESIMVNR NKLYYVLYSS LPSKPLSVKD

LYKRLTIQA
Length:609
Mass (Da):70,354
Last modified:October 1, 1996 - v1
Checksum:i24BE9884EEC0E478
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66864 Genomic DNA. Translation: AAB17870.1.
Z73001 Genomic DNA. Translation: CAA97243.1.
BK006941 Genomic DNA. Translation: DAA08310.1.
PIRiS64539.
RefSeqiNP_011732.1. NM_001181345.1.

Genome annotation databases

EnsemblFungiiYGR216C; YGR216C; YGR216C.
GeneIDi853130.
KEGGisce:YGR216C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66864 Genomic DNA. Translation: AAB17870.1.
Z73001 Genomic DNA. Translation: CAA97243.1.
BK006941 Genomic DNA. Translation: DAA08310.1.
PIRiS64539.
RefSeqiNP_011732.1. NM_001181345.1.

3D structure databases

ProteinModelPortaliP53306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33469. 74 interactors.
DIPiDIP-7773N.
IntActiP53306. 2 interactors.
MINTiMINT-1365234.

Proteomic databases

MaxQBiP53306.
PRIDEiP53306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR216C; YGR216C; YGR216C.
GeneIDi853130.
KEGGisce:YGR216C.

Organism-specific databases

EuPathDBiFungiDB:YGR216C.
SGDiS000003448. GPI1.

Phylogenomic databases

GeneTreeiENSGT00390000004994.
HOGENOMiHOG000000940.
InParanoidiP53306.
KOiK03860.
OMAiALINHFP.
OrthoDBiEOG092C5W2N.

Enzyme and pathway databases

UniPathwayiUPA00196.
BioCyciYEAST:G3O-30898-MONOMER.

Miscellaneous databases

PROiP53306.

Family and domain databases

InterProiIPR007720. GlcNAc_Gpi1.
[Graphical view]
PfamiPF05024. Gpi1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPI1_YEAST
AccessioniPrimary (citable) accession number: P53306
Secondary accession number(s): D6VUZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.