Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53301

- CRH1_YEAST

UniProt

P53301 - CRH1_YEAST

Protein

Probable glycosidase CRH1

Gene

CRH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei134 – 1341NucleophileBy similarity
    Active sitei138 – 1381Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: SGD

    GO - Biological processi

    1. cell wall chitin metabolic process Source: SGD
    2. fungal-type cell wall organization Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30879-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glycosidase CRH1 (EC:3.2.-.-)
    Alternative name(s):
    Congo red hypersensitive protein 1
    Gene namesi
    Name:CRH1
    Ordered Locus Names:YGR189C
    ORF Names:G7553
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR189c.
    SGDiS000003421. CRH1.

    Subcellular locationi

    Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Found at the incipient bud site, around the septum area in later stages of budding, and in ascospore envelopes. Redistributes uniformly over the cell cortex upon heat stress.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-KW
    3. fungal-type cell wall Source: SGD
    4. incipient cellular bud site Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 482460Probable glycosidase CRH1PRO_0000045430Add
    BLAST
    Propeptidei483 – 50725Removed in mature formSequence AnalysisPRO_0000045431Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
    Lipidationi482 – 4821GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP53301.
    PaxDbiP53301.

    Expressioni

    Inductioni

    Positively regulated by cell integrity signaling through MPK1 in response to cell wall perturbation. Induced by heat stress.3 Publications

    Gene expression databases

    GenevestigatoriP53301.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GET3Q121541EBI-23440,EBI-2989
    PEX29Q033701EBI-23440,EBI-36170
    YHR080CP388001EBI-23440,EBI-24597

    Protein-protein interaction databases

    BioGridi33442. 12 interactions.
    DIPiDIP-4360N.
    IntActiP53301. 1 interaction.
    MINTiMINT-475521.
    STRINGi4932.YGR189C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53301.
    SMRiP53301. Positions 57-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi63 – 664Poly-Ser
    Compositional biasi301 – 481181Ser-richAdd
    BLAST
    Compositional biasi301 – 31010Poly-Ser
    Compositional biasi345 – 35713Poly-SerAdd
    BLAST
    Compositional biasi387 – 3915Poly-Ser
    Compositional biasi467 – 4704Poly-Ser

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    GeneTreeiENSGT00610000086657.
    HOGENOMiHOG000196187.
    OMAiLENFHTY.
    OrthoDBiEOG7RNK9W.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR017168. Glyco_hydro_16_CRH1_prd.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53301-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVLDLLTVL SASSLLSTFA AAESTATADS TTAASSTASC NPLKTTGCTP    50
    DTALATSFSE DFSSSSKWFT DLKHAGEIKY GSDGLSMTLA KRYDNPSLKS 100
    NFYIMYGKLE VILKAANGTG IVSSFYLQSD DLDEIDIEWV GGDNTQFQSN 150
    FFSKGDTTTY DRGEFHGVDT PTDKFHNYTL DWAMDKTTWY LDGESVRVLS 200
    NTSSEGYPQS PMYLMMGIWA GGDPDNAAGT IEWAGGETNY NDAPFTMYIE 250
    KVIVTDYSTG KKYTYGDQSG SWESIEADGG SIYGRYDQAQ EDFAVLANGG 300
    SISSSSTSSS TVSSSASSTV SSSVSSTVSS SASSTVSSSV SSTVSSSSSV 350
    SSSSSTSPSS STATSSKTLA SSSVTTSSSI SSFEKQSSSS SKKTVASSST 400
    SESIISSTKT PATVSSTTRS TVAPTTQQSS VSSDSPVQDK GGVATSSNDV 450
    TSSTTQISSK YTSTIQSSSS EASSTNSVQI SNGADLAQSL PREGKLFSVL 500
    VALLALL 507
    Length:507
    Mass (Da):52,758
    Last modified:October 1, 1996 - v1
    Checksum:i7D7B61F57AEA942C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72974 Genomic DNA. Translation: CAA97215.1.
    X99074 Genomic DNA. Translation: CAA67525.1.
    BK006941 Genomic DNA. Translation: DAA08283.1.
    PIRiS64507.
    RefSeqiNP_011705.1. NM_001181318.1.

    Genome annotation databases

    EnsemblFungiiYGR189C; YGR189C; YGR189C.
    GeneIDi853102.
    KEGGisce:YGR189C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72974 Genomic DNA. Translation: CAA97215.1 .
    X99074 Genomic DNA. Translation: CAA67525.1 .
    BK006941 Genomic DNA. Translation: DAA08283.1 .
    PIRi S64507.
    RefSeqi NP_011705.1. NM_001181318.1.

    3D structure databases

    ProteinModelPortali P53301.
    SMRi P53301. Positions 57-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33442. 12 interactions.
    DIPi DIP-4360N.
    IntActi P53301. 1 interaction.
    MINTi MINT-475521.
    STRINGi 4932.YGR189C.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    MaxQBi P53301.
    PaxDbi P53301.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR189C ; YGR189C ; YGR189C .
    GeneIDi 853102.
    KEGGi sce:YGR189C.

    Organism-specific databases

    CYGDi YGR189c.
    SGDi S000003421. CRH1.

    Phylogenomic databases

    eggNOGi COG2273.
    GeneTreei ENSGT00610000086657.
    HOGENOMi HOG000196187.
    OMAi LENFHTY.
    OrthoDBi EOG7RNK9W.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30879-MONOMER.

    Miscellaneous databases

    NextBioi 973104.

    Gene expression databases

    Genevestigatori P53301.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR017168. Glyco_hydro_16_CRH1_prd.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037299. Glycosidase_CRH1_prd. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
      Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
      Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."
      Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.
      Mol. Gen. Genet. 258:53-59(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway."
      Jung U.S., Levin D.E.
      Mol. Microbiol. 34:1049-1057(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "A novel family of cell wall-related proteins regulated differently during the yeast life cycle."
      Rodriguez-Pena J.M., Cid V.J., Arroyo J., Nombela C.
      Mol. Cell. Biol. 20:3245-3255(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae."
      Terashima H., Yabuki N., Arisawa M., Hamada K., Kitada K.
      Mol. Gen. Genet. 264:64-74(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, SUBCELLULAR LOCATION.
    8. "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
      Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
      J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, GPI-ANCHOR.
    9. "Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae."
      Yin Q.Y., de Groot P.W.J., de Jong L., Klis F.M., de Koster C.G.
      FEMS Yeast Res. 7:887-896(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: LEVEL OF PROTEIN EXPRESSION, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall."
      Cabib E., Blanco N., Grau C., Rodriguez-Pena J.M., Arroyo J.
      Mol. Microbiol. 63:921-935(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HEAT STRESS.

    Entry informationi

    Entry nameiCRH1_YEAST
    AccessioniPrimary (citable) accession number: P53301
    Secondary accession number(s): D6VUX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 44000 wall-bound molecules/cell in log phase YPD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3