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P53301

- CRH1_YEAST

UniProt

P53301 - CRH1_YEAST

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Protein

Probable glycosidase CRH1

Gene

CRH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei134 – 1341NucleophileBy similarity
Active sitei138 – 1381Proton donorBy similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: SGD

GO - Biological processi

  1. cell wall chitin metabolic process Source: SGD
  2. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-30879-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycosidase CRH1 (EC:3.2.-.-)
Alternative name(s):
Congo red hypersensitive protein 1
Gene namesi
Name:CRH1
Ordered Locus Names:YGR189C
ORF Names:G7553
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR189c.
SGDiS000003421. CRH1.

Subcellular locationi

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Found at the incipient bud site, around the septum area in later stages of budding, and in ascospore envelopes. Redistributes uniformly over the cell cortex upon heat stress.

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
  4. incipient cellular bud site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 482460Probable glycosidase CRH1PRO_0000045430Add
BLAST
Propeptidei483 – 50725Removed in mature formSequence AnalysisPRO_0000045431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
Lipidationi482 – 4821GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP53301.
PaxDbiP53301.

Expressioni

Inductioni

Positively regulated by cell integrity signaling through MPK1 in response to cell wall perturbation. Induced by heat stress.3 Publications

Gene expression databases

GenevestigatoriP53301.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121541EBI-23440,EBI-2989
PEX29Q033701EBI-23440,EBI-36170
YHR080CP388001EBI-23440,EBI-24597

Protein-protein interaction databases

BioGridi33442. 12 interactions.
DIPiDIP-4360N.
IntActiP53301. 1 interaction.
MINTiMINT-475521.
STRINGi4932.YGR189C.

Structurei

3D structure databases

ProteinModelPortaliP53301.
SMRiP53301. Positions 55-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 664Poly-Ser
Compositional biasi301 – 481181Ser-richAdd
BLAST
Compositional biasi301 – 31010Poly-Ser
Compositional biasi345 – 35713Poly-SerAdd
BLAST
Compositional biasi387 – 3915Poly-Ser
Compositional biasi467 – 4704Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
GeneTreeiENSGT00610000086657.
HOGENOMiHOG000196187.
InParanoidiP53301.
OMAiLENFHTY.
OrthoDBiEOG7RNK9W.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR017168. Glyco_hydro_16_CRH1_prd.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53301-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLDLLTVL SASSLLSTFA AAESTATADS TTAASSTASC NPLKTTGCTP
60 70 80 90 100
DTALATSFSE DFSSSSKWFT DLKHAGEIKY GSDGLSMTLA KRYDNPSLKS
110 120 130 140 150
NFYIMYGKLE VILKAANGTG IVSSFYLQSD DLDEIDIEWV GGDNTQFQSN
160 170 180 190 200
FFSKGDTTTY DRGEFHGVDT PTDKFHNYTL DWAMDKTTWY LDGESVRVLS
210 220 230 240 250
NTSSEGYPQS PMYLMMGIWA GGDPDNAAGT IEWAGGETNY NDAPFTMYIE
260 270 280 290 300
KVIVTDYSTG KKYTYGDQSG SWESIEADGG SIYGRYDQAQ EDFAVLANGG
310 320 330 340 350
SISSSSTSSS TVSSSASSTV SSSVSSTVSS SASSTVSSSV SSTVSSSSSV
360 370 380 390 400
SSSSSTSPSS STATSSKTLA SSSVTTSSSI SSFEKQSSSS SKKTVASSST
410 420 430 440 450
SESIISSTKT PATVSSTTRS TVAPTTQQSS VSSDSPVQDK GGVATSSNDV
460 470 480 490 500
TSSTTQISSK YTSTIQSSSS EASSTNSVQI SNGADLAQSL PREGKLFSVL

VALLALL
Length:507
Mass (Da):52,758
Last modified:October 1, 1996 - v1
Checksum:i7D7B61F57AEA942C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72974 Genomic DNA. Translation: CAA97215.1.
X99074 Genomic DNA. Translation: CAA67525.1.
BK006941 Genomic DNA. Translation: DAA08283.1.
PIRiS64507.
RefSeqiNP_011705.1. NM_001181318.1.

Genome annotation databases

EnsemblFungiiYGR189C; YGR189C; YGR189C.
GeneIDi853102.
KEGGisce:YGR189C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72974 Genomic DNA. Translation: CAA97215.1 .
X99074 Genomic DNA. Translation: CAA67525.1 .
BK006941 Genomic DNA. Translation: DAA08283.1 .
PIRi S64507.
RefSeqi NP_011705.1. NM_001181318.1.

3D structure databases

ProteinModelPortali P53301.
SMRi P53301. Positions 55-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33442. 12 interactions.
DIPi DIP-4360N.
IntActi P53301. 1 interaction.
MINTi MINT-475521.
STRINGi 4932.YGR189C.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

MaxQBi P53301.
PaxDbi P53301.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR189C ; YGR189C ; YGR189C .
GeneIDi 853102.
KEGGi sce:YGR189C.

Organism-specific databases

CYGDi YGR189c.
SGDi S000003421. CRH1.

Phylogenomic databases

eggNOGi COG2273.
GeneTreei ENSGT00610000086657.
HOGENOMi HOG000196187.
InParanoidi P53301.
OMAi LENFHTY.
OrthoDBi EOG7RNK9W.

Enzyme and pathway databases

BioCyci YEAST:G3O-30879-MONOMER.

Miscellaneous databases

NextBioi 973104.

Gene expression databases

Genevestigatori P53301.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR017168. Glyco_hydro_16_CRH1_prd.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PIRSFi PIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
    Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
    Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."
    Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.
    Mol. Gen. Genet. 258:53-59(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway."
    Jung U.S., Levin D.E.
    Mol. Microbiol. 34:1049-1057(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "A novel family of cell wall-related proteins regulated differently during the yeast life cycle."
    Rodriguez-Pena J.M., Cid V.J., Arroyo J., Nombela C.
    Mol. Cell. Biol. 20:3245-3255(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae."
    Terashima H., Yabuki N., Arisawa M., Hamada K., Kitada K.
    Mol. Gen. Genet. 264:64-74(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION.
  8. "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
    Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
    J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, GPI-ANCHOR.
  9. "Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae."
    Yin Q.Y., de Groot P.W.J., de Jong L., Klis F.M., de Koster C.G.
    FEMS Yeast Res. 7:887-896(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: LEVEL OF PROTEIN EXPRESSION, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall."
    Cabib E., Blanco N., Grau C., Rodriguez-Pena J.M., Arroyo J.
    Mol. Microbiol. 63:921-935(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HEAT STRESS.

Entry informationi

Entry nameiCRH1_YEAST
AccessioniPrimary (citable) accession number: P53301
Secondary accession number(s): D6VUX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 44000 wall-bound molecules/cell in log phase YPD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3