Probable glycosidase CRH1 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53301 (CRH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable glycosidase CRH1
EC=3.2.-.-

Alternative name(s):
Congo red hypersensitive protein 1

Gene names

Name:	CRH1
Ordered Locus Names:	YGR189C
ORF Names:	G7553

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall. Ref.6 Ref.10
Subcellular location	Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Found at the incipient bud site, around the septum area in later stages of budding, and in ascospore envelopes. Redistributes uniformly over the cell cortex upon heat stress. Ref.4 Ref.6 Ref.7 Ref.8 Ref.10
Induction	Positively regulated by cell integrity signaling through MPK1 in response to cell wall perturbation. Induced by heat stress. Ref.5 Ref.7 Ref.10
Post-translational modification	The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
Miscellaneous	Present with 44000 wall-bound molecules/cell in log phase YPD medium. Ref.9
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. CRH1 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall chitin metabolic process Inferred from mutant phenotype PubMed 18694928. Source: SGD fungal-type cell wall organization Inferred from mutant phenotype PubMed 18694928. Source: SGD
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay Ref.6 Ref.8. Source: SGD incipient cellular bud site Inferred from direct assay Ref.6. Source: SGD
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from mutant phenotype PubMed 18694928. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
GET3	Q12154	1	EBI-23440,EBI-2989
PEX29	Q03370	1	EBI-23440,EBI-36170
YHR080C	P38800	1	EBI-23440,EBI-24597

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

Potential

Chain

23 – 482

460

Probable glycosidase CRH1

PRO_0000045430

Propeptide

483 – 507

Removed in mature form Potential

PRO_0000045431

Regions

Compositional bias

63 – 66

Poly-Ser

Compositional bias

301 – 481

181

Ser-rich

Compositional bias

301 – 310

Poly-Ser

Compositional bias

345 – 357

Poly-Ser

Compositional bias

387 – 391

Poly-Ser

Compositional bias

467 – 470

Poly-Ser

Sites

Active site

134

Nucleophile By similarity

Active site

138

Proton donor By similarity

Amino acid modifications

Lipidation

482

GPI-anchor amidated asparagine Potential

Glycosylation

117

N-linked (GlcNAc...) Potential

Glycosylation

177

N-linked (GlcNAc...) Potential

Glycosylation

201

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P53301 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7D7B61F57AEA942C
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FASTA

507

52,758

        10         20         30         40         50         60 
MKVLDLLTVL SASSLLSTFA AAESTATADS TTAASSTASC NPLKTTGCTP DTALATSFSE 

        70         80         90        100        110        120 
DFSSSSKWFT DLKHAGEIKY GSDGLSMTLA KRYDNPSLKS NFYIMYGKLE VILKAANGTG 

       130        140        150        160        170        180 
IVSSFYLQSD DLDEIDIEWV GGDNTQFQSN FFSKGDTTTY DRGEFHGVDT PTDKFHNYTL 

       190        200        210        220        230        240 
DWAMDKTTWY LDGESVRVLS NTSSEGYPQS PMYLMMGIWA GGDPDNAAGT IEWAGGETNY 

       250        260        270        280        290        300 
NDAPFTMYIE KVIVTDYSTG KKYTYGDQSG SWESIEADGG SIYGRYDQAQ EDFAVLANGG 

       310        320        330        340        350        360 
SISSSSTSSS TVSSSASSTV SSSVSSTVSS SASSTVSSSV SSTVSSSSSV SSSSSTSPSS 

       370        380        390        400        410        420 
STATSSKTLA SSSVTTSSSI SSFEKQSSSS SKKTVASSST SESIISSTKT PATVSSTTRS 

       430        440        450        460        470        480 
TVAPTTQQSS VSSDSPVQDK GGVATSSNDV TSSTTQISSK YTSTIQSSSS EASSTNSVQI 

       490        500 
SNGADLAQSL PREGKLFSVL VALLALL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII." Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C. Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae." Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K. Mol. Gen. Genet. 258:53-59(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway." Jung U.S., Levin D.E. Mol. Microbiol. 34:1049-1057(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"A novel family of cell wall-related proteins regulated differently during the yeast life cycle." Rodriguez-Pena J.M., Cid V.J., Arroyo J., Nombela C. Mol. Cell. Biol. 20:3245-3255(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae." Terashima H., Yabuki N., Arisawa M., Hamada K., Kitada K. Mol. Gen. Genet. 264:64-74(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, SUBCELLULAR LOCATION.
[8]	"Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages." Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G. J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, PROBABLE GPI-ANCHOR.
[9]	"Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae." Yin Q.Y., de Groot P.W.J., de Jong L., Klis F.M., de Koster C.G. FEMS Yeast Res. 7:887-896(2007) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION, MASS SPECTROMETRY.
[10]	"Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall." Cabib E., Blanco N., Grau C., Rodriguez-Pena J.M., Arroyo J. Mol. Microbiol. 63:921-935(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HEAT STRESS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72974 Genomic DNA. Translation: CAA97215.1. X99074 Genomic DNA. Translation: CAA67525.1. BK006941 Genomic DNA. Translation: DAA08283.1.
PIR	S64507.
RefSeq	NP_011705.1. NM_001181318.1.
3D structure databases
ProteinModelPortal	P53301.
SMR	P53301. Positions 57-287.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4360N.
IntAct	P53301. 1 interaction.
MINT	MINT-475521.
STRING	4932.YGR189C.
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Proteomic databases
PaxDb	P53301.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR189C; YGR189C; YGR189C.
GeneID	853102.
KEGG	sce:YGR189C.
Organism-specific databases
CYGD	YGR189c.
SGD	S000003421. CRH1.
Phylogenomic databases
eggNOG	COG2273.
GeneTree	ENSGT00610000086657.
HOGENOM	HOG000196187.
OMA	AGTIEWA.
OrthoDB	EOG4VT962.
Gene expression databases
Genevestigator	P53301.
GermOnline	YGR189C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR017168. Glyco_hydro_16_CRH1_prd. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PIRSF	PIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	973104.

Entry information

Entry name

CRH1_YEAST

Accession

Primary (citable) accession number: P53301
Secondary accession number(s): D6VUX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents