Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53299

- TIM13_YEAST

UniProt

P53299 - TIM13_YEAST

Protein

Mitochondrial import inner membrane translocase subunit TIM13

Gene

TIM13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins under precise conditions while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins. Strictly required for import of TIM23 in some conditions, when a low membrane potential exists in the mitochondria.5 Publications

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein transporter activity Source: SGD

    GO - Biological processi

    1. protein import into mitochondrial inner membrane Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30873-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM13
    Gene namesi
    Name:TIM13
    Ordered Locus Names:YGR181W
    ORF Names:G7157
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR181w.
    SGDiS000003413. TIM13.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. mitochondrial intermembrane space Source: SGD
    3. mitochondrial intermembrane space protein transporter complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi57 – 571C → S: Abolishes import into mitochondrion; when associated with S-61. 1 Publication
    Mutagenesisi61 – 611C → S: Abolishes import into mitochondrion; when associated with S-57. 1 Publication
    Mutagenesisi73 – 731C → S: Abolishes import into mitochondrion; when associated with S-77. 1 Publication
    Mutagenesisi77 – 771C → S: Abolishes import into mitochondrion; when associated with S-73. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 105105Mitochondrial import inner membrane translocase subunit TIM13PRO_0000193632Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi57 ↔ 77Curated
    Disulfide bondi61 ↔ 73Curated

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP53299.
    PaxDbiP53299.
    PeptideAtlasiP53299.

    Expressioni

    Gene expression databases

    GenevestigatoriP53299.

    Interactioni

    Subunit structurei

    Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TIM8P577444EBI-9121,EBI-9013892

    Protein-protein interaction databases

    BioGridi33434. 91 interactions.
    DIPiDIP-5547N.
    IntActiP53299. 6 interactions.
    MINTiMINT-563654.
    STRINGi4932.YGR181W.

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi47 – 6115
    Helixi71 – 9323

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3CJHX-ray2.60A/C/E/G/I/K42-105[»]
    ProteinModelPortaliP53299.
    SMRiP53299. Positions 46-99.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53299.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 7721Twin CX3C motifAdd
    BLAST

    Domaini

    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.1 Publication

    Sequence similaritiesi

    Belongs to the small Tim family.Curated

    Phylogenomic databases

    eggNOGiNOG246901.
    GeneTreeiENSGT00390000014000.
    HOGENOMiHOG000115759.
    KOiK17781.
    OMAiRTYIARI.
    OrthoDBiEOG751NV3.

    Family and domain databases

    Gene3Di1.10.287.810. 1 hit.
    InterProiIPR004217. Tim10/DDP_fam_Znf.
    [Graphical view]
    PfamiPF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view]
    SUPFAMiSSF144122. SSF144122. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53299-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLSSIFGGG APSQQKEAAT TAKTTPNPIA KELKNQIAQE LAVANATELV    50
    NKISENCFEK CLTSPYATRN DACIDQCLAK YMRSWNVISK AYISRIQNAS 100
    ASGEI 105
    Length:105
    Mass (Da):11,286
    Last modified:October 1, 1996 - v1
    Checksum:iB1DDFE87AFDC52A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99074 Genomic DNA. Translation: CAA67526.1.
    Z72966 Genomic DNA. Translation: CAA97207.1.
    AY558508 Genomic DNA. Translation: AAS56834.1.
    BK006941 Genomic DNA. Translation: DAA08276.1.
    PIRiS64495.
    RefSeqiNP_011697.3. NM_001181310.3.

    Genome annotation databases

    EnsemblFungiiYGR181W; YGR181W; YGR181W.
    GeneIDi853093.
    KEGGisce:YGR181W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99074 Genomic DNA. Translation: CAA67526.1 .
    Z72966 Genomic DNA. Translation: CAA97207.1 .
    AY558508 Genomic DNA. Translation: AAS56834.1 .
    BK006941 Genomic DNA. Translation: DAA08276.1 .
    PIRi S64495.
    RefSeqi NP_011697.3. NM_001181310.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3CJH X-ray 2.60 A/C/E/G/I/K 42-105 [» ]
    ProteinModelPortali P53299.
    SMRi P53299. Positions 46-99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33434. 91 interactions.
    DIPi DIP-5547N.
    IntActi P53299. 6 interactions.
    MINTi MINT-563654.
    STRINGi 4932.YGR181W.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P53299.
    PaxDbi P53299.
    PeptideAtlasi P53299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR181W ; YGR181W ; YGR181W .
    GeneIDi 853093.
    KEGGi sce:YGR181W.

    Organism-specific databases

    CYGDi YGR181w.
    SGDi S000003413. TIM13.

    Phylogenomic databases

    eggNOGi NOG246901.
    GeneTreei ENSGT00390000014000.
    HOGENOMi HOG000115759.
    KOi K17781.
    OMAi RTYIARI.
    OrthoDBi EOG751NV3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30873-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    EvolutionaryTracei P53299.
    NextBioi 973081.
    PROi P53299.

    Gene expression databases

    Genevestigatori P53299.

    Family and domain databases

    Gene3Di 1.10.287.810. 1 hit.
    InterProi IPR004217. Tim10/DDP_fam_Znf.
    [Graphical view ]
    Pfami PF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF144122. SSF144122. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
      Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
      Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Different import pathways through the mitochondrial intermembrane space for inner membrane proteins."
      Leuenberger D., Bally N.A., Schatz G., Koehler C.M.
      EMBO J. 18:4816-4822(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THE TIM22 COMPLEX.
    6. "The role of the TIM8-13 complex in the import of Tim23 into mitochondria."
      Paschen S.A., Rothbauer U., Kaldi K., Bauer M.F., Neupert W., Brunner M.
      EMBO J. 19:6392-6400(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria."
      Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E.
      J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex."
      Murphy M.P., Leuenberger D., Curran S.P., Oppliger W., Koehler C.M.
      Mol. Cell. Biol. 21:6132-6138(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins."
      Curran S.P., Leuenberger D., Schmidt E., Koehler C.M.
      J. Cell Biol. 158:1017-1027(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, PROBABLE DISULFIDE BONDS, LACK OF ZINC-BINDING WHEN PRESENT IN THE MITOCHONDRIAL INTERMEMBRANE SPACE.
    10. "Import of small Tim proteins into the mitochondrial intermembrane space."
      Lutz T., Neupert W., Herrmann J.M.
      EMBO J. 22:4400-4408(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ZINC-BINDING WHEN PRESENT IN THE CYTOSOL, DOMAIN, MUTAGENESIS OF CYS-57; CYS-61; CYS-73 AND CYS-77.

    Entry informationi

    Entry nameiTIM13_YEAST
    AccessioniPrimary (citable) accession number: P53299
    Secondary accession number(s): D6VUW5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3