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P53299 (TIM13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM13
Gene names
Name:TIM13
Ordered Locus Names:YGR181W
ORF Names:G7157
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length105 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins under precise conditions while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins. Strictly required for import of TIM23 in some conditions, when a low membrane potential exists in the mitochondria. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure

Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit. Ref.5 Ref.9

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side Ref.5.

Domain

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane. Ref.10

Sequence similarities

Belongs to the small Tim family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TIM8P577444EBI-9121,EBI-9013892

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 105105Mitochondrial import inner membrane translocase subunit TIM13
PRO_0000193632

Regions

Motif57 – 7721Twin CX3C motif

Amino acid modifications

Disulfide bond57 ↔ 77 Probable
Disulfide bond61 ↔ 73 Probable

Experimental info

Mutagenesis571C → S: Abolishes import into mitochondrion; when associated with S-61. Ref.10
Mutagenesis611C → S: Abolishes import into mitochondrion; when associated with S-57. Ref.10
Mutagenesis731C → S: Abolishes import into mitochondrion; when associated with S-77. Ref.10
Mutagenesis771C → S: Abolishes import into mitochondrion; when associated with S-73. Ref.10

Secondary structure

..... 105
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53299 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B1DDFE87AFDC52A3

FASTA10511,286
        10         20         30         40         50         60 
MGLSSIFGGG APSQQKEAAT TAKTTPNPIA KELKNQIAQE LAVANATELV NKISENCFEK 

        70         80         90        100 
CLTSPYATRN DACIDQCLAK YMRSWNVISK AYISRIQNAS ASGEI 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII."
Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C.
Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Different import pathways through the mitochondrial intermembrane space for inner membrane proteins."
Leuenberger D., Bally N.A., Schatz G., Koehler C.M.
EMBO J. 18:4816-4822(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THE TIM22 COMPLEX.
[6]"The role of the TIM8-13 complex in the import of Tim23 into mitochondria."
Paschen S.A., Rothbauer U., Kaldi K., Bauer M.F., Neupert W., Brunner M.
EMBO J. 19:6392-6400(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria."
Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E.
J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex."
Murphy M.P., Leuenberger D., Curran S.P., Oppliger W., Koehler C.M.
Mol. Cell. Biol. 21:6132-6138(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins."
Curran S.P., Leuenberger D., Schmidt E., Koehler C.M.
J. Cell Biol. 158:1017-1027(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, PROBABLE DISULFIDE BONDS, LACK OF ZINC-BINDING WHEN PRESENT IN THE MITOCHONDRIAL INTERMEMBRANE SPACE.
[10]"Import of small Tim proteins into the mitochondrial intermembrane space."
Lutz T., Neupert W., Herrmann J.M.
EMBO J. 22:4400-4408(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ZINC-BINDING WHEN PRESENT IN THE CYTOSOL, DOMAIN, MUTAGENESIS OF CYS-57; CYS-61; CYS-73 AND CYS-77.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99074 Genomic DNA. Translation: CAA67526.1.
Z72966 Genomic DNA. Translation: CAA97207.1.
AY558508 Genomic DNA. Translation: AAS56834.1.
BK006941 Genomic DNA. Translation: DAA08276.1.
PIRS64495.
RefSeqNP_011697.3. NM_001181310.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CJHX-ray2.60A/C/E/G/I/K42-105[»]
ProteinModelPortalP53299.
SMRP53299. Positions 46-99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33434. 91 interactions.
DIPDIP-5547N.
IntActP53299. 6 interactions.
MINTMINT-563654.
STRING4932.YGR181W.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBP53299.
PaxDbP53299.
PeptideAtlasP53299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR181W; YGR181W; YGR181W.
GeneID853093.
KEGGsce:YGR181W.

Organism-specific databases

CYGDYGR181w.
SGDS000003413. TIM13.

Phylogenomic databases

eggNOGNOG246901.
GeneTreeENSGT00390000014000.
HOGENOMHOG000115759.
KOK17781.
OMARTYIARI.
OrthoDBEOG751NV3.

Enzyme and pathway databases

BioCycYEAST:G3O-30873-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).

Gene expression databases

GenevestigatorP53299.

Family and domain databases

Gene3D1.10.287.810. 1 hit.
InterProIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMSSF144122. SSF144122. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP53299.
NextBio973081.
PROP53299.

Entry information

Entry nameTIM13_YEAST
AccessionPrimary (citable) accession number: P53299
Secondary accession number(s): D6VUW5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references