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Protein

Mitochondrial import inner membrane translocase subunit TIM13

Gene

TIM13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins under precise conditions while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins. Strictly required for import of TIM23 in some conditions, when a low membrane potential exists in the mitochondria.5 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein transporter activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Translocation, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30873-MONOMER

Protein family/group databases

TCDBi3.A.8.1.1 the mitochondrial protein translocase (mpt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM13
Gene namesi
Name:TIM13
Ordered Locus Names:YGR181W
ORF Names:G7157
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR181W
SGDiS000003413 TIM13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57C → S: Abolishes import into mitochondrion; when associated with S-61. 1 Publication1
Mutagenesisi61C → S: Abolishes import into mitochondrion; when associated with S-57. 1 Publication1
Mutagenesisi73C → S: Abolishes import into mitochondrion; when associated with S-77. 1 Publication1
Mutagenesisi77C → S: Abolishes import into mitochondrion; when associated with S-73. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936321 – 105Mitochondrial import inner membrane translocase subunit TIM13Add BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 77Curated
Disulfide bondi61 ↔ 73Curated

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP53299
PaxDbiP53299
PRIDEiP53299

PTM databases

iPTMnetiP53299

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM8P577444EBI-9121,EBI-9013892

Protein-protein interaction databases

BioGridi33434168 interactors.
DIPiDIP-5547N
IntActiP53299 6 interactors.
MINTiP53299
STRINGi4932.YGR181W

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 61Combined sources15
Helixi71 – 93Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CJHX-ray2.60A/C/E/G/I/K42-105[»]
ProteinModelPortaliP53299
SMRiP53299
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53299

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 77Twin CX3C motifAdd BLAST21

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane.1 Publication

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014000
HOGENOMiHOG000115759
InParanoidiP53299
KOiK17781
OMAiSYNLVAR
OrthoDBiEOG092C5RBL

Family and domain databases

Gene3Di1.10.287.8101 hit
InterProiView protein in InterPro
IPR004217 Tim10-like
IPR035427 Tim10-like_dom_sf
PfamiView protein in Pfam
PF02953 zf-Tim10_DDP, 1 hit
SUPFAMiSSF144122 SSF144122, 1 hit

Sequencei

Sequence statusi: Complete.

P53299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSSIFGGG APSQQKEAAT TAKTTPNPIA KELKNQIAQE LAVANATELV
60 70 80 90 100
NKISENCFEK CLTSPYATRN DACIDQCLAK YMRSWNVISK AYISRIQNAS

ASGEI
Length:105
Mass (Da):11,286
Last modified:October 1, 1996 - v1
Checksum:iB1DDFE87AFDC52A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99074 Genomic DNA Translation: CAA67526.1
Z72966 Genomic DNA Translation: CAA97207.1
AY558508 Genomic DNA Translation: AAS56834.1
BK006941 Genomic DNA Translation: DAA08276.1
PIRiS64495
RefSeqiNP_011697.3, NM_001181310.3

Genome annotation databases

EnsemblFungiiYGR181W; YGR181W; YGR181W
GeneIDi853093
KEGGisce:YGR181W

Similar proteinsi

Entry informationi

Entry nameiTIM13_YEAST
AccessioniPrimary (citable) accession number: P53299
Secondary accession number(s): D6VUW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 28, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome