Mitochondrial import inner membrane translocase subunit TIM13 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53299 (TIM13_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM13

Gene names

Name:	TIM13
Ordered Locus Names:	YGR181W
ORF Names:	G7157

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins under precise conditions while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins. Strictly required for import of TIM23 in some conditions, when a low membrane potential exists in the mitochondria. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit. Ref.5 Ref.9
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side Ref.5.
Domain	The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane. Ref.10
Sequence similarities	Belongs to the small Tim family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial inner membrane Inferred from direct assay PubMed 10051608 Ref.5. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space protein transporter complex Inferred from direct assay Ref.9. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein transporter activity Inferred from direct assay PubMed 10051608 Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 105

105

Mitochondrial import inner membrane translocase subunit TIM13

PRO_0000193632

Regions

Motif

57 – 77

Twin CX3C motif

Amino acid modifications

Disulfide bond

57 ↔ 77

Probable

Disulfide bond

61 ↔ 73

Probable

Experimental info

Mutagenesis

C → S: Abolishes import into mitochondrion; when associated with S-61. Ref.10

Mutagenesis

C → S: Abolishes import into mitochondrion; when associated with S-57. Ref.10

Mutagenesis

C → S: Abolishes import into mitochondrion; when associated with S-77. Ref.10

Mutagenesis

C → S: Abolishes import into mitochondrion; when associated with S-73. Ref.10

Secondary structure

105

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P53299 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B1DDFE87AFDC52A3
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FASTA

105

11,286

        10         20         30         40         50         60 
MGLSSIFGGG APSQQKEAAT TAKTTPNPIA KELKNQIAQE LAVANATELV NKISENCFEK 

        70         80         90        100 
CLTSPYATRN DACIDQCLAK YMRSWNVISK AYISRIQNAS ASGEI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of a 23,002 bp DNA fragment of the right arm of Saccharomyces cerevisiae chromosome VII." Arroyo J., Garcia-Gonzalez M., Garcia-Saez M.I., Sanchez-Perez M., Nombela C. Yeast 13:357-363(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Different import pathways through the mitochondrial intermembrane space for inner membrane proteins." Leuenberger D., Bally N.A., Schatz G., Koehler C.M. EMBO J. 18:4816-4822(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THE TIM22 COMPLEX.
[6]	"The role of the TIM8-13 complex in the import of Tim23 into mitochondria." Paschen S.A., Rothbauer U., Kaldi K., Bauer M.F., Neupert W., Brunner M. EMBO J. 19:6392-6400(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria." Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E. J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex." Murphy M.P., Leuenberger D., Curran S.P., Oppliger W., Koehler C.M. Mol. Cell. Biol. 21:6132-6138(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins." Curran S.P., Leuenberger D., Schmidt E., Koehler C.M. J. Cell Biol. 158:1017-1027(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, PROBABLE DISULFIDE BONDS, LACK OF ZINC-BINDING WHEN PRESENT IN THE MITOCHONDRIAL INTERMEMBRANE SPACE.
[10]	"Import of small Tim proteins into the mitochondrial intermembrane space." Lutz T., Neupert W., Herrmann J.M. EMBO J. 22:4400-4408(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ZINC-BINDING WHEN PRESENT IN THE CYTOSOL, DOMAIN, MUTAGENESIS OF CYS-57; CYS-61; CYS-73 AND CYS-77.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X99074 Genomic DNA. Translation: CAA67526.1.
Z72966 Genomic DNA. Translation: CAA97207.1.
AY558508 Genomic DNA. Translation: AAS56834.1.
BK006941 Genomic DNA. Translation: DAA08276.1.

PIR

S64495.

RefSeq

NP_011697.3. NM_001181310.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CJH	X-ray	2.60	A/C/E/G/I/K	42-105	[»]

ProteinModelPortal

P53299.

SMR

P53299. Positions 46-99.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5547N.

IntAct

P53299. 3 interactions.

MINT

MINT-563654.

STRING

4932.YGR181W.

Protein family/group databases

TCDB

3.A.8.1.1. mitochondrial protein translocase (MPT) family.

Proteomic databases

PaxDb

P53299.

PeptideAtlas

P53299.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR181W; YGR181W; YGR181W.

GeneID

853093.

KEGG

sce:YGR181W.
sce:YGR188C.

Organism-specific databases

CYGD

YGR181w.

SGD

S000003413. TIM13.

Phylogenomic databases

eggNOG

NOG246901.

GeneTree

ENSGT00390000014000.

HOGENOM

HOG000115759.

K02178.

OMA

CLAKYMR.

OrthoDB

EOG4T1MX6.

Enzyme and pathway databases

Reactome

REACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

Genevestigator

P53299.

GermOnline

YGR181W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.287.810. 1 hit.

InterPro

IPR004217. Tim10/DDP_fam_Znf.
[Graphical view]

Pfam

PF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]

SUPFAM

SSF144122. SSF144122. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P53299.

NextBio

973081.

Entry information

Entry name

TIM13_YEAST

Accession

Primary (citable) accession number: P53299
Secondary accession number(s): D6VUW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents