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P53298

- OKP1_YEAST

UniProt

P53298 - OKP1_YEAST

Protein

Central kinetochore subunit OKP1

Gene

OKP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore Source: SGD
    2. chromosome segregation Source: SGD
    3. meiotic nuclear division Source: UniProtKB-KW
    4. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30872-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Central kinetochore subunit OKP1
    Alternative name(s):
    Outer kinetochore protein 1
    Gene namesi
    Name:OKP1
    Ordered Locus Names:YGR179C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR179c.
    SGDiS000003411. OKP1.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. COMA complex Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Central kinetochore subunit OKP1PRO_0000058039Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei70 – 701Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53298.
    PaxDbiP53298.

    Expressioni

    Gene expression databases

    GenevestigatoriP53298.

    Interactioni

    Subunit structurei

    Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DAM1P532672EBI-23429,EBI-23268

    Protein-protein interaction databases

    BioGridi33431. 53 interactions.
    DIPiDIP-2382N.
    IntActiP53298. 20 interactions.
    MINTiMINT-479300.
    STRINGi4932.YGR179C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53298.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili239 – 28547Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG47499.
    KOiK11555.
    OMAiNDSHELN.
    OrthoDBiEOG7ZKSQ3.

    Sequencei

    Sequence statusi: Complete.

    P53298-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAADRDNFLQ NIENDSINNG QAMDLSPNRS SSESDSSILM NVNDIKTLRL    50
    DVAPEAKSTQ SKKSLFYENS DDAEEGEIEE RTNKEEGQYH HKGSKQLRFE 100
    VGKESTGKLQ SHLSDGSATS GEGNVRPWEF RKVIQAEYRE RLPRNYELKH 150
    WKKPSKIMIG SILRLLETNT VSALDSVFEK YEKEMNQMTH GDNNEVKRIY 200
    SKKERLLEII LTKIKKKLRQ AKFPSRISER DLDIEYIYSK RQFIQNRYSQ 250
    ELQNNERLEA ILSREQNLLE ETRKLCMNLK TNNKKRLTEK LIQKDLHPVL 300
    NKAMEYTYGL ESTNGFMHPD GPVTFRNDSH ELNLMLNDPI KSTADVRLDK 350
    EEVLSLLPSL KEYTKKSKEL KETMGQMISD SHEEEIKEVF VPHHESHQDK 400
    TEEDIH 406
    Length:406
    Mass (Da):47,349
    Last modified:October 1, 1996 - v1
    Checksum:iF664346B37EEFFF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72964 Genomic DNA. Translation: CAA97205.1.
    AY557837 Genomic DNA. Translation: AAS56163.1.
    BK006941 Genomic DNA. Translation: DAA08274.1.
    PIRiS64493.
    RefSeqiNP_011695.1. NM_001181308.1.

    Genome annotation databases

    EnsemblFungiiYGR179C; YGR179C; YGR179C.
    GeneIDi853090.
    KEGGisce:YGR179C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72964 Genomic DNA. Translation: CAA97205.1 .
    AY557837 Genomic DNA. Translation: AAS56163.1 .
    BK006941 Genomic DNA. Translation: DAA08274.1 .
    PIRi S64493.
    RefSeqi NP_011695.1. NM_001181308.1.

    3D structure databases

    ProteinModelPortali P53298.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33431. 53 interactions.
    DIPi DIP-2382N.
    IntActi P53298. 20 interactions.
    MINTi MINT-479300.
    STRINGi 4932.YGR179C.

    Proteomic databases

    MaxQBi P53298.
    PaxDbi P53298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR179C ; YGR179C ; YGR179C .
    GeneIDi 853090.
    KEGGi sce:YGR179C.

    Organism-specific databases

    CYGDi YGR179c.
    SGDi S000003411. OKP1.

    Phylogenomic databases

    eggNOGi NOG47499.
    KOi K11555.
    OMAi NDSHELN.
    OrthoDBi EOG7ZKSQ3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30872-MONOMER.

    Miscellaneous databases

    NextBioi 973073.

    Gene expression databases

    Genevestigatori P53298.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore."
      Ortiz J., Stemmann O., Rank S., Lechner J.
      Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CTF19 AND MCM21, SUBCELLULAR LOCATION.
    5. "Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
      Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
      Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
    6. "Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes."
      De Wulf P., McAinsh A.D., Sorger P.K.
      Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF COMA COMPLEX.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOKP1_YEAST
    AccessioniPrimary (citable) accession number: P53298
    Secondary accession number(s): D6VUW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2688 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3