P53298 (OKP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Central kinetochore subunit OKP1

Alternative name(s):
Outer kinetochore protein 1

Gene names

Name:	OKP1
Ordered Locus Names:	YGR179C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.
Subunit structure	Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Associated with kinetochores. Ref.4 Ref.7
Miscellaneous	Present with 2688 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	attachment of spindle microtubules to kinetochore Inferred from mutant phenotype PubMed 19597337. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	COMA complex Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAM1	P53267	2	EBI-23429,EBI-23268

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Central kinetochore subunit OKP1		PRO_0000058039
Regions
	Coiled coil	239 – 285	47	Potential
Amino acid modifications
	Modified residue	70	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P53298 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F664346B37EEFFF5	FASTA	406	47,349
	10 20 30 40 50 60 MAADRDNFLQ NIENDSINNG QAMDLSPNRS SSESDSSILM NVNDIKTLRL DVAPEAKSTQ 70 80 90 100 110 120 SKKSLFYENS DDAEEGEIEE RTNKEEGQYH HKGSKQLRFE VGKESTGKLQ SHLSDGSATS 130 140 150 160 170 180 GEGNVRPWEF RKVIQAEYRE RLPRNYELKH WKKPSKIMIG SILRLLETNT VSALDSVFEK 190 200 210 220 230 240 YEKEMNQMTH GDNNEVKRIY SKKERLLEII LTKIKKKLRQ AKFPSRISER DLDIEYIYSK 250 260 270 280 290 300 RQFIQNRYSQ ELQNNERLEA ILSREQNLLE ETRKLCMNLK TNNKKRLTEK LIQKDLHPVL 310 320 330 340 350 360 NKAMEYTYGL ESTNGFMHPD GPVTFRNDSH ELNLMLNDPI KSTADVRLDK EEVLSLLPSL 370 380 390 400 KEYTKKSKEL KETMGQMISD SHEEEIKEVF VPHHESHQDK TEEDIH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore." Ortiz J., Stemmann O., Rank S., Lechner J. Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CTF19 AND MCM21, SUBCELLULAR LOCATION.
[5]	"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p." Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G. Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
[6]	"Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes." De Wulf P., McAinsh A.D., Sorger P.K. Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF COMA COMPLEX.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72964 Genomic DNA. Translation: CAA97205.1. AY557837 Genomic DNA. Translation: AAS56163.1. BK006941 Genomic DNA. Translation: DAA08274.1.
PIR	S64493.
RefSeq	NP_011695.1. NM_001181308.1.
3D structure databases
ProteinModelPortal	P53298.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2382N.
IntAct	P53298. 20 interactions.
MINT	MINT-479300.
STRING	4932.YGR179C.
Proteomic databases
PaxDb	P53298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR179C; YGR179C; YGR179C.
GeneID	853090.
KEGG	sce:YGR179C.
Organism-specific databases
CYGD	YGR179c.
SGD	S000003411. OKP1.
Phylogenomic databases
eggNOG	NOG47499.
KO	K11555.
OrthoDB	EOG4HTD2W.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30872-MONOMER.
Gene expression databases
Genevestigator	P53298.
GermOnline	YGR179C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	973073.

Entry information

Entry name

OKP1_YEAST

Accession

Primary (citable) accession number: P53298
Secondary accession number(s): D6VUW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program