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P53298

- OKP1_YEAST

UniProt

P53298 - OKP1_YEAST

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Protein

Central kinetochore subunit OKP1

Gene

OKP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

GO - Biological processi

  1. attachment of spindle microtubules to kinetochore Source: SGD
  2. chromosome segregation Source: SGD
  3. meiotic nuclear division Source: UniProtKB-KW
  4. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-30872-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit OKP1
Alternative name(s):
Outer kinetochore protein 1
Gene namesi
Name:OKP1
Ordered Locus Names:YGR179C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR179c.
SGDiS000003411. OKP1.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Associated with kinetochores.

GO - Cellular componenti

  1. COMA complex Source: SGD
  2. condensed nuclear chromosome kinetochore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Central kinetochore subunit OKP1PRO_0000058039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53298.
PaxDbiP53298.

Expressioni

Gene expression databases

GenevestigatoriP53298.

Interactioni

Subunit structurei

Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1. The COMA subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
DAM1P532672EBI-23429,EBI-23268

Protein-protein interaction databases

BioGridi33431. 53 interactions.
DIPiDIP-2382N.
IntActiP53298. 20 interactions.
MINTiMINT-479300.
STRINGi4932.YGR179C.

Structurei

3D structure databases

ProteinModelPortaliP53298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili239 – 28547Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG47499.
InParanoidiP53298.
KOiK11555.
OMAiNDSHELN.
OrthoDBiEOG7ZKSQ3.

Sequencei

Sequence statusi: Complete.

P53298-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAADRDNFLQ NIENDSINNG QAMDLSPNRS SSESDSSILM NVNDIKTLRL
60 70 80 90 100
DVAPEAKSTQ SKKSLFYENS DDAEEGEIEE RTNKEEGQYH HKGSKQLRFE
110 120 130 140 150
VGKESTGKLQ SHLSDGSATS GEGNVRPWEF RKVIQAEYRE RLPRNYELKH
160 170 180 190 200
WKKPSKIMIG SILRLLETNT VSALDSVFEK YEKEMNQMTH GDNNEVKRIY
210 220 230 240 250
SKKERLLEII LTKIKKKLRQ AKFPSRISER DLDIEYIYSK RQFIQNRYSQ
260 270 280 290 300
ELQNNERLEA ILSREQNLLE ETRKLCMNLK TNNKKRLTEK LIQKDLHPVL
310 320 330 340 350
NKAMEYTYGL ESTNGFMHPD GPVTFRNDSH ELNLMLNDPI KSTADVRLDK
360 370 380 390 400
EEVLSLLPSL KEYTKKSKEL KETMGQMISD SHEEEIKEVF VPHHESHQDK

TEEDIH
Length:406
Mass (Da):47,349
Last modified:October 1, 1996 - v1
Checksum:iF664346B37EEFFF5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72964 Genomic DNA. Translation: CAA97205.1.
AY557837 Genomic DNA. Translation: AAS56163.1.
BK006941 Genomic DNA. Translation: DAA08274.1.
PIRiS64493.
RefSeqiNP_011695.1. NM_001181308.1.

Genome annotation databases

EnsemblFungiiYGR179C; YGR179C; YGR179C.
GeneIDi853090.
KEGGisce:YGR179C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72964 Genomic DNA. Translation: CAA97205.1 .
AY557837 Genomic DNA. Translation: AAS56163.1 .
BK006941 Genomic DNA. Translation: DAA08274.1 .
PIRi S64493.
RefSeqi NP_011695.1. NM_001181308.1.

3D structure databases

ProteinModelPortali P53298.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33431. 53 interactions.
DIPi DIP-2382N.
IntActi P53298. 20 interactions.
MINTi MINT-479300.
STRINGi 4932.YGR179C.

Proteomic databases

MaxQBi P53298.
PaxDbi P53298.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR179C ; YGR179C ; YGR179C .
GeneIDi 853090.
KEGGi sce:YGR179C.

Organism-specific databases

CYGDi YGR179c.
SGDi S000003411. OKP1.

Phylogenomic databases

eggNOGi NOG47499.
InParanoidi P53298.
KOi K11555.
OMAi NDSHELN.
OrthoDBi EOG7ZKSQ3.

Enzyme and pathway databases

BioCyci YEAST:G3O-30872-MONOMER.

Miscellaneous databases

NextBioi 973073.

Gene expression databases

Genevestigatori P53298.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore."
    Ortiz J., Stemmann O., Rank S., Lechner J.
    Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CTF19 AND MCM21, SUBCELLULAR LOCATION.
  5. "Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
    Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
    Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
  6. "Hierarchical assembly of the budding yeast kinetochore from multiple subcomplexes."
    De Wulf P., McAinsh A.D., Sorger P.K.
    Genes Dev. 17:2902-2921(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF COMA COMPLEX.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOKP1_YEAST
AccessioniPrimary (citable) accession number: P53298
Secondary accession number(s): D6VUW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2688 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3