P53297 (PBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PAB1-binding protein 1

Gene names

Name:	PBP1
Synonyms:	MRS16
Ordered Locus Names:	YGR178C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	722 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Appears to promote proper polyadenylation. In the absence of PBP1, the 3'termini of pre-mRNAs are properly cleaved but lack full-length poly(A) tails. May act to repress the ability of PAB1 to negatively regulate polyadenylation. Negative regulator of poly(A) nuclease (PAN) activity. Ref.4 Ref.9
Subunit structure	Interacts with FIR1, IGO1, LSM12, PBP4 and PAB1. Ref.4 Ref.6 Ref.9 Ref.13
Subcellular location	Cytoplasm. Nucleus. Mitochondrion Ref.5 Ref.8.
Miscellaneous	Present with 2870 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ataxin-2 family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA polyadenylation Inferred from physical interaction Ref.4. Source: SGD positive regulation of translation Inferred from mutant phenotype PubMed 15082763. Source: SGD stress granule assembly Inferred from mutant phenotype PubMed 18981231. Source: SGD
Cellular_component	cytoplasmic stress granule Inferred from direct assay PubMed 18981231. Source: SGD mitochondrion Inferred from direct assay Ref.8PubMed 16823961. Source: SGD nucleus Inferred from direct assay PubMed 11914276Ref.5. Source: SGD polysome Inferred from direct assay PubMed 15082763. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSM12	P38828	6	EBI-12961,EBI-24700

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 722	722	PAB1-binding protein 1		PRO_0000058244
Amino acid modifications
	Modified residue	106	1	Phosphoserine Ref.10 Ref.12
	Modified residue	215	1	Phosphoserine Ref.12
	Modified residue	436	1	Phosphoserine Ref.10 Ref.12
	Modified residue	442	1	Phosphoserine Ref.12
	Modified residue	451	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P53297 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 92005F3A2346193E	FASTA	722	78,781
	10 20 30 40 50 60 MKGNFRKRDS STNSRKGGNS DSNYTNGGVP NQNNSSMFYE NPEITRNFDD RQDYLLANSI 70 80 90 100 110 120 GSDVTVTVTS GVKYTGLLVS CNLESTNGID VVLRFPRVAD SGVSDSVDDL AKTLGETLLI 130 140 150 160 170 180 HGEDVAELEL KNIDLSLDEK WENSKAQETT PARTNIEKER VNGESNEVTK FRTDVDISGS 190 200 210 220 230 240 GREIKERKLE KWTPEEGAEH FDINKGKALE DDSASWDQFA VNEKKFGVKS TFDEHLYTTK 250 260 270 280 290 300 INKDDPNYSK RLQEAERIAK EIESQGTSGN IHIAEDRGII IDDSGLDEED LYSGVDRRGD 310 320 330 340 350 360 ELLAALKSNS KPNSNKGNRY VPPTLRQQPH HMDPAIISSS NSNKNENAVS TDTSTPAAAG 370 380 390 400 410 420 APEGKPPQKT SKNKKSLSSK EAQIEELKKF SEKFKVPYDI PKDMLEVLKR SSSTLKSNSS 430 440 450 460 470 480 LPPKPISKTP SAKTVSPTTQ ISAGKSESRR SGSNISQGQS STGHTTRSST SLRRRNHGSF 490 500 510 520 530 540 FGAKNPHTND AKRVLFGKSF NMFIKSKEAH DEKKKGDDAS ENMEPFFIEK PYFTAPTWLN 550 560 570 580 590 600 TIEESYKTFF PDEDTAIQEA QTRFQQRQLN SMGNAVPGMN PAMGMNMGGM MGFPMGGPSA 610 620 630 640 650 660 SPNPMMNGFA AGSMGMYMPF QPQPMFYHPS MPQMMPVMGS NGAEEGGGNI SPHVPAGFMA 670 680 690 700 710 720 AGPGAPMGAF GYPGGIPFQG MMGSGPSGMP ANGSAMHSHG HSRNYHQTSH HGHHNSSTSG HK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and characterization of a suppressor of the pet- phenotype in a Saccharomyces cerevisiae strain without mitochondrial group II introns." Mecklenbrauker I. Thesis (1996), Vienna Biocentre, Austria Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44774 / DBY747.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation." Mangus D.A., Amrani N., Jacobson A. Mol. Cell. Biol. 18:7383-7396(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAB1.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LSM12 AND PBP4, IDENTIFICATION BY MASS SPECTROMETRY.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[9]	"Identification of factors regulating poly(A) tail synthesis and maturation." Mangus D.A., Smith M.M., McSweeney J.M., Jacobson A. Mol. Cell. Biol. 24:4196-4206(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FIR1 AND PBP4.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-436, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; SER-215; SER-436 AND SER-442, MASS SPECTROMETRY.
[13]	"Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway." Talarek N., Cameroni E., Jaquenoud M., Luo X., Bontron S., Lippman S., Devgan G., Snyder M., Broach J.R., De Virgilio C. Mol. Cell 38:345-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IGO1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46931 Genomic DNA. Translation: AAB94294.1. Z72963 Genomic DNA. Translation: CAA97204.1. BK006941 Genomic DNA. Translation: DAA08273.1.
PIR	S64492.
RefSeq	NP_011694.3. NM_001181307.3.
3D structure databases
ProteinModelPortal	P53297.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2464N.
IntAct	P53297. 20 interactions.
MINT	MINT-488669.
STRING	4932.YGR178C.
Proteomic databases
PaxDb	P53297.
PeptideAtlas	P53297.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR178C; YGR178C; YGR178C.
GeneID	853089.
KEGG	sce:YGR178C. sce:YGR185C.
Organism-specific databases
CYGD	YGR178c.
SGD	S000003410. PBP1.
Phylogenomic databases
eggNOG	COG5180.
HOGENOM	HOG000093792.
KO	K01866.
OMA	IEKPYFT.
OrthoDB	EOG4643MM.
Gene expression databases
Genevestigator	P53297.
GermOnline	YGR178C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009604. LsmAD_domain. IPR025852. SM_dom_ATX. [Graphical view]
Pfam	PF06741. LsmAD. 1 hit. PF14438. SM-ATX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973070.

Entry information

Entry name

PBP1_YEAST

Accession

Primary (citable) accession number: P53297
Secondary accession number(s): D6VUW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program