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P53297 (PBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PAB1-binding protein 1
Gene names
Name:PBP1
Synonyms:MRS16
Ordered Locus Names:YGR178C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to promote proper polyadenylation. In the absence of PBP1, the 3'termini of pre-mRNAs are properly cleaved but lack full-length poly(A) tails. May act to repress the ability of PAB1 to negatively regulate polyadenylation. Negative regulator of poly(A) nuclease (PAN) activity. Ref.4 Ref.9

Subunit structure

Interacts with FIR1, IGO1, LSM12, PBP4 and PAB1. Ref.4 Ref.6 Ref.9 Ref.13

Subcellular location

Cytoplasm. Nucleus. Mitochondrion Ref.5 Ref.8.

Miscellaneous

Present with 2870 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ataxin-2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LSM12P388286EBI-12961,EBI-24700

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 722722PAB1-binding protein 1
PRO_0000058244

Amino acid modifications

Modified residue1061Phosphoserine Ref.10 Ref.11 Ref.12
Modified residue1931Phosphothreonine Ref.12
Modified residue2151Phosphoserine Ref.11
Modified residue4361Phosphoserine Ref.10 Ref.11

Sequences

Sequence LengthMass (Da)Tools
P53297 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 92005F3A2346193E

FASTA72278,781
        10         20         30         40         50         60 
MKGNFRKRDS STNSRKGGNS DSNYTNGGVP NQNNSSMFYE NPEITRNFDD RQDYLLANSI 

        70         80         90        100        110        120 
GSDVTVTVTS GVKYTGLLVS CNLESTNGID VVLRFPRVAD SGVSDSVDDL AKTLGETLLI 

       130        140        150        160        170        180 
HGEDVAELEL KNIDLSLDEK WENSKAQETT PARTNIEKER VNGESNEVTK FRTDVDISGS 

       190        200        210        220        230        240 
GREIKERKLE KWTPEEGAEH FDINKGKALE DDSASWDQFA VNEKKFGVKS TFDEHLYTTK 

       250        260        270        280        290        300 
INKDDPNYSK RLQEAERIAK EIESQGTSGN IHIAEDRGII IDDSGLDEED LYSGVDRRGD 

       310        320        330        340        350        360 
ELLAALKSNS KPNSNKGNRY VPPTLRQQPH HMDPAIISSS NSNKNENAVS TDTSTPAAAG 

       370        380        390        400        410        420 
APEGKPPQKT SKNKKSLSSK EAQIEELKKF SEKFKVPYDI PKDMLEVLKR SSSTLKSNSS 

       430        440        450        460        470        480 
LPPKPISKTP SAKTVSPTTQ ISAGKSESRR SGSNISQGQS STGHTTRSST SLRRRNHGSF 

       490        500        510        520        530        540 
FGAKNPHTND AKRVLFGKSF NMFIKSKEAH DEKKKGDDAS ENMEPFFIEK PYFTAPTWLN 

       550        560        570        580        590        600 
TIEESYKTFF PDEDTAIQEA QTRFQQRQLN SMGNAVPGMN PAMGMNMGGM MGFPMGGPSA 

       610        620        630        640        650        660 
SPNPMMNGFA AGSMGMYMPF QPQPMFYHPS MPQMMPVMGS NGAEEGGGNI SPHVPAGFMA 

       670        680        690        700        710        720 
AGPGAPMGAF GYPGGIPFQG MMGSGPSGMP ANGSAMHSHG HSRNYHQTSH HGHHNSSTSG 


HK 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and characterization of a suppressor of the pet- phenotype in a Saccharomyces cerevisiae strain without mitochondrial group II introns."
Mecklenbrauker I.
Thesis (1996), Vienna Biocentre, Austria
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 44774 / DBY747.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation."
Mangus D.A., Amrani N., Jacobson A.
Mol. Cell. Biol. 18:7383-7396(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PAB1.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes."
Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J.
Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LSM12 AND PBP4, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[9]"Identification of factors regulating poly(A) tail synthesis and maturation."
Mangus D.A., Smith M.M., McSweeney J.M., Jacobson A.
Mol. Cell. Biol. 24:4196-4206(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FIR1 AND PBP4.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-436, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106; SER-215 AND SER-436, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND THR-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway."
Talarek N., Cameroni E., Jaquenoud M., Luo X., Bontron S., Lippman S., Devgan G., Snyder M., Broach J.R., De Virgilio C.
Mol. Cell 38:345-355(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IGO1.
[14]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46931 Genomic DNA. Translation: AAB94294.1.
Z72963 Genomic DNA. Translation: CAA97204.1.
BK006941 Genomic DNA. Translation: DAA08273.1.
PIRS64492.
RefSeqNP_011694.3. NM_001181307.3.

3D structure databases

ProteinModelPortalP53297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33430. 126 interactions.
DIPDIP-2464N.
IntActP53297. 23 interactions.
MINTMINT-488669.
STRING4932.YGR178C.

Proteomic databases

MaxQBP53297.
PaxDbP53297.
PeptideAtlasP53297.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR178C; YGR178C; YGR178C.
GeneID853089.
KEGGsce:YGR178C.

Organism-specific databases

CYGDYGR178c.
SGDS000003410. PBP1.

Phylogenomic databases

eggNOGCOG5180.
GeneTreeENSGT00530000063565.
HOGENOMHOG000093792.
OMAIEKPYFT.
OrthoDBEOG7TF7K9.

Enzyme and pathway databases

BioCycYEAST:G3O-30871-MONOMER.

Gene expression databases

GenevestigatorP53297.

Family and domain databases

InterProIPR009604. LsmAD_domain.
IPR025852. SM_dom_ATX.
[Graphical view]
PfamPF06741. LsmAD. 1 hit.
PF14438. SM-ATX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973070.

Entry information

Entry namePBP1_YEAST
AccessionPrimary (citable) accession number: P53297
Secondary accession number(s): D6VUW2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families