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Protein

Alcohol O-acetyltransferase 2

Gene

ATF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can use acetyl-CoA to synthesize acetate esters from various alcohols, producing ethyl acetate, isoamyl acetate, isobutyl acetate, butyl acetate, hexyl acetate, heptyl acetate and octyl acetate (PubMed:12957907, PubMed:12937998, PubMed:16845703, PubMed:17891501). ATF2 seems to play only a minor role in the acetate ester synthesis, compared to ATF1 (PubMed:12957907). Plays an active role in the detoxification hydroxysteroids and possibly certain phytochemicals, in association with the efflux pumps PDR5 and SNQ2 (PubMed:10103065).5 Publications

Miscellaneous

Present with 3000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + an alcohol = CoA + an acetyl ester.1 Publication

Kineticsi

  1. KM=0.52 µM for pregnenolone1 Publication
  2. KM=1.1 µM for acetyl-CoA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei189Charge relay systemBy similarity1
    Active sitei193Charge relay systemBy similarity1

    GO - Molecular functioni

    • alcohol O-acetyltransferase activity Source: SGD

    GO - Biological processi

    • acetate ester metabolic process involved in fermentation Source: SGD
    • response to toxic substance Source: SGD
    • steroid metabolic process Source: SGD
    • sterol acetylation Source: SGD

    Keywordsi

    Molecular functionAcyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:YGR177C-MONOMER
    YEAST:YGR177C-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alcohol O-acetyltransferase 21 Publication (EC:2.3.1.841 Publication)
    Short name:
    AATase 21 Publication
    Alternative name(s):
    Acetyl-CoA:pregnenolone acetyltransferase1 Publication
    Short name:
    APAT1 Publication
    Gene namesi
    Name:ATF21 Publication
    Ordered Locus Names:YGR177C
    ORF Names:G7135
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGR177C
    SGDiS000003409 ATF2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum, Lipid droplet, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Results in an 18% decrease in isoamyl acetate formation and causes a 13% reduction of ethyl acetate formation (PubMed:12957907). Leads to the complete elimination of acetyl-CoA:pregnenolone acetyltransferase activity and consequently abolishes the esterification of pregnenolone and increases the toxicity of pregnenolone (PubMed:10103065).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000647211 – 535Alcohol O-acetyltransferase 2Add BLAST535

    Proteomic databases

    MaxQBiP53296
    PaxDbiP53296
    PRIDEiP53296

    Interactioni

    Protein-protein interaction databases

    BioGridi33429, 52 interactors
    DIPiDIP-5544N
    IntActiP53296, 6 interactors
    MINTiP53296
    STRINGi4932.YGR177C

    Structurei

    3D structure databases

    ProteinModelPortaliP53296
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni19 – 36Membrane association1 PublicationAdd BLAST18
    Regioni515 – 532Membrane association1 PublicationAdd BLAST18

    Domaini

    Segments of the N- and C-terminal domains (residues 19-36 and 515-532, respectively) are predicted to be amphipathic helices and are essential for endoplasmic reticulum membrane association (PubMed:25093817).1 Publication

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000000257
    HOGENOMiHOG000034125
    InParanoidiP53296
    KOiK00664
    OMAiRMGHLEN
    OrthoDBiEOG092C1I3C

    Family and domain databases

    InterProiView protein in InterPro
    IPR010828 Atf2/Sli1-like
    PfamiView protein in Pfam
    PF07247 AATase, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P53296-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEDIEGYEPH ITQELIDRGH ARRMGHLENY FAVLSRQKMY SNFTVYAELN
    60 70 80 90 100
    KGVNKRQLML VLKVLLQKYS TLAHTIIPKH YPHHEAYYSS EEYLSKPFPQ
    110 120 130 140 150
    HDFIKVISHL EFDDLIMNNQ PEYREVMEKI SEQFKKDDFK VTNRLIELIS
    160 170 180 190 200
    PVIIPLGNPK RPNWRLICLP GKDTDGFETW KNFVYVTNHC GSDGVSGSNF
    210 220 230 240 250
    FKDLALLFCK IEEKGFDYDE EFIEDQVIID YDRDYTEISK LPKPITDRID
    260 270 280 290 300
    YKPALTSLPK FFLTTFIYEH CNFKTSSEST LTARYSPSSN ANASYNYLLH
    310 320 330 340 350
    FSTKQVEQIR AQIKKNVHDG CTLTPFIQAC FLVALYRLDK LFTKSLLEYG
    360 370 380 390 400
    FDVAIPSNAR RFLPNDEELR DSYKYGSNVG GSHYAYLISS FDIPEGDNDK
    410 420 430 440 450
    FWSLVEYYYD RFLESYDNGD HLIGLGVLQL DFIVENKNID SLLANSYLHQ
    460 470 480 490 500
    QRGGAIISNT GLVSQDTTKP YYVRDLIFSQ SAGALRFAFG LNVCSTNVNG
    510 520 530
    MNMDMSVVQG TLRDRGEWES FCKLFYQTIG EFASL
    Length:535
    Mass (Da):61,898
    Last modified:October 1, 1996 - v1
    Checksum:iD615241A7DF1ECD5
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti179T → A in AAU09735 (PubMed:17322287).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z72961 Genomic DNA Translation: CAA97203.1
    AY723818 Genomic DNA Translation: AAU09735.1
    BK006941 Genomic DNA Translation: DAA08272.1
    PIRiS64491
    RefSeqiNP_011693.1, NM_001181306.1

    Genome annotation databases

    EnsemblFungiiYGR177C; YGR177C; YGR177C
    GeneIDi853088
    KEGGisce:YGR177C

    Similar proteinsi

    Entry informationi

    Entry nameiATF2_YEAST
    AccessioniPrimary (citable) accession number: P53296
    Secondary accession number(s): D6VUW1, E9P955
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: May 23, 2018
    This is version 121 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health