P53295 (RBG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-interacting GTPase 2

Alternative name(s):
GTP-binding protein RBG2

Gene names

Name:	RBG2
Synonyms:	GIR1
Ordered Locus Names:	YGR173W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in ribosomal function. Ref.6
Subcellular location	Cytoplasm Ref.3.
Miscellaneous	Present with 6200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from genetic interaction PubMed 21076151. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.3. Source: SGD
Molecular_function	GTP binding Inferred from sequence or structural similarity PubMed 10760581. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GIR2	Q03768	6	EBI-23421,EBI-7618

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Ribosome-interacting GTPase 2		PRO_0000205450
Regions
	Domain	75 – 173	99	G
	Nucleotide binding	70 – 77	8	GTP By similarity
	Nucleotide binding	116 – 120	5	GTP By similarity
	Nucleotide binding	250 – 253	4	GTP By similarity
Amino acid modifications
	Modified residue	364	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53295 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D144569C9C5D777C	FASTA	368	41,006
	10 20 30 40 50 60 MGIIDKIKAI EEEMARTQKN KATEHHLGLL KGKLARYRQQ LLADEAGSGG GGGSGFEVAK 70 80 90 100 110 120 SGDARVVLIG YPSVGKSSLL GKITTTKSEI AHYAFTTLTS VPGVLKYQGA EIQIVDLPGI 130 140 150 160 170 180 IYGASQGKGR GRQVVATART ADLVLMVLDA TKSEHQRASL EKELENVGIR LNKEKPNIYY 190 200 210 220 230 240 KKKETGGVKV TFTSPPKTNL TEQAIKMILR DYRIHNAEVL VRDDQCTIDD FIDVINEQHR 250 260 270 280 290 300 NYVKCLYVYN KIDAVSLEEV DKLAREPNTV VMSCEMDLGL QDVVEEIWYQ LNLSRVYTKK 310 320 330 340 350 360 RGVRPVFDDP LVVRNNSTIG DLCHGIHRDF KDKFKYALVW GSSAKHSPQK CGLNHRIDDE DVVSLFAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, MASS SPECTROMETRY.
[6]	"Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1." Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R. Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72958 Genomic DNA. Translation: CAA97199.1. BK006941 Genomic DNA. Translation: DAA08268.1.
PIR	S64487.
RefSeq	NP_011689.1. NM_001181302.1.
3D structure databases
ProteinModelPortal	P53295.
SMR	P53295. Positions 64-229, 291-368.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2827N.
IntAct	P53295. 17 interactions.
MINT	MINT-405787.
STRING	4932.YGR173W.
Proteomic databases
PaxDb	P53295.
PeptideAtlas	P53295.
PRIDE	P53295.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR173W; YGR173W; YGR173W.
GeneID	853083.
KEGG	sce:YGR173W.
Organism-specific databases
CYGD	YGR173w.
SGD	S000003405. RBG2.
Phylogenomic databases
eggNOG	COG1163.
GeneTree	ENSGT00550000075059.
KO	K06944.
OMA	LFREDCT.
OrthoDB	EOG4W6S4M.
Gene expression databases
Genevestigator	P53295.
GermOnline	YGR173W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006074. GTP1-OBG_CS. IPR006073. GTP_binding_domain. IPR005225. Small_GTP-bd_dom. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
Pfam	PF01926. MMR_HSR1. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
PRINTS	PR00326. GTP1OBG.
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS00905. GTP1_OBG. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973056.

Entry information

Entry name

RBG2_YEAST

Accession

Primary (citable) accession number: P53295
Secondary accession number(s): D6VUV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program