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P53295 (RBG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-interacting GTPase 2
Alternative name(s):
GTP-binding protein RBG2
Gene names
Name:RBG2
Synonyms:GIR1
Ordered Locus Names:YGR173W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in ribosomal function. Ref.6

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 6200 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.

Contains 1 OBG-type G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcytoplasmic translation

Inferred from genetic interaction PubMed 21076151. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from sequence or structural similarity PubMed 10760581. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GIR2Q037685EBI-23421,EBI-7618

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368Ribosome-interacting GTPase 2
PRO_0000205450

Regions

Domain64 – 292229OBG-type G
Nucleotide binding70 – 778GTP By similarity
Nucleotide binding116 – 1205GTP By similarity
Nucleotide binding250 – 2534GTP By similarity

Amino acid modifications

Modified residue3641Phosphoserine Ref.5 Ref.7

Sequences

Sequence LengthMass (Da)Tools
P53295 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D144569C9C5D777C

FASTA36841,006
        10         20         30         40         50         60 
MGIIDKIKAI EEEMARTQKN KATEHHLGLL KGKLARYRQQ LLADEAGSGG GGGSGFEVAK 

        70         80         90        100        110        120 
SGDARVVLIG YPSVGKSSLL GKITTTKSEI AHYAFTTLTS VPGVLKYQGA EIQIVDLPGI 

       130        140        150        160        170        180 
IYGASQGKGR GRQVVATART ADLVLMVLDA TKSEHQRASL EKELENVGIR LNKEKPNIYY 

       190        200        210        220        230        240 
KKKETGGVKV TFTSPPKTNL TEQAIKMILR DYRIHNAEVL VRDDQCTIDD FIDVINEQHR 

       250        260        270        280        290        300 
NYVKCLYVYN KIDAVSLEEV DKLAREPNTV VMSCEMDLGL QDVVEEIWYQ LNLSRVYTKK 

       310        320        330        340        350        360 
RGVRPVFDDP LVVRNNSTIG DLCHGIHRDF KDKFKYALVW GSSAKHSPQK CGLNHRIDDE 


DVVSLFAK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact on polyribosomes with Gcn1."
Wout P.K., Sattlegger E., Sullivan S.M., Maddock J.R.
Eukaryot. Cell 8:1061-1071(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-364, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72958 Genomic DNA. Translation: CAA97199.1.
BK006941 Genomic DNA. Translation: DAA08268.1.
PIRS64487.
RefSeqNP_011689.1. NM_001181302.1.

3D structure databases

ProteinModelPortalP53295.
SMRP53295. Positions 2-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33425. 28 interactions.
DIPDIP-2827N.
IntActP53295. 16 interactions.
MINTMINT-405787.
STRING4932.YGR173W.

Proteomic databases

PaxDbP53295.
PeptideAtlasP53295.
PRIDEP53295.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR173W; YGR173W; YGR173W.
GeneID853083.
KEGGsce:YGR173W.

Organism-specific databases

CYGDYGR173w.
SGDS000003405. RBG2.

Phylogenomic databases

eggNOGCOG1163.
GeneTreeENSGT00550000075059.
KOK06944.
OMAKVMEAKT.
OrthoDBEOG70S7GB.

Enzyme and pathway databases

BioCycYEAST:G3O-30869-MONOMER.

Gene expression databases

GenevestigatorP53295.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR006074. GTP1-OBG_CS.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamPF01926. MMR_HSR1. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
PRINTSPR00326. GTP1OBG.
SUPFAMSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973056.
PROP53295.

Entry information

Entry nameRBG2_YEAST
AccessionPrimary (citable) accession number: P53295
Secondary accession number(s): D6VUV7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families