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Protein

tRNA pseudouridine(31) synthase

Gene

PUS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of pseudouridine at position 31 in the psi GC loop of tRNAS.1 Publication

Catalytic activityi

tRNA uridine(31) = tRNA pseudouridine(31).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • tRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16704.
YEAST:YGR169C-MONOMER.
BRENDAi5.4.99.42. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(31) synthase (EC:5.4.99.42)
Alternative name(s):
Pseudouridine synthase 6
Pseudouridylate synthase 6
Uracil hydrolyase
tRNA pseudouridine 31 synthase
Short name:
PSI31 synthase
Gene namesi
Name:PUS6
Ordered Locus Names:YGR169C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR169C.
SGDiS000003401. PUS6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi168 – 1681D → A: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404tRNA pseudouridine(31) synthasePRO_0000162757Add
BLAST

Proteomic databases

MaxQBiP53294.

PTM databases

iPTMnetiP53294.

Interactioni

Protein-protein interaction databases

BioGridi33421. 8 interactions.
DIPiDIP-5429N.
MINTiMINT-544439.

Structurei

3D structure databases

ProteinModelPortaliP53294.
SMRiP53294. Positions 121-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000048339.
HOGENOMiHOG000167783.
InParanoidiP53294.
KOiK15453.
OMAiCYRLDKI.
OrthoDBiEOG7M0P19.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
PROSITEiPS01129. PSI_RLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIKVIEVY TQNGLRKVRP YYNRRSAFVK GRWLGKLLID VLVSEFKLRP
60 70 80 90 100
RAYYLDQIRK GTYRLIRDGV PLVPDHLMTT IIKNHDVLET TTHKHEPPVK
110 120 130 140 150
QWCSQEVEAE DLPGRIAGFN IVFEDESILV IDKPSGIPVH PTGQFYQNTI
160 170 180 190 200
TELLKLHGVD ALPCYRLDKI TSGLLILAKN SQSAGEIQKS IRSRDMIKIY
210 220 230 240 250
LARVKGRFPH SELILDNENA AETTFEDTSK VTVEMTPIYS IDPKRQFPVG
260 270 280 290 300
LSTSKDAITK FYPIRYFSHA DETVVACKPI TGRTHQIRIH LARLGHPIVN
310 320 330 340 350
DSVYCSHITK YPERLKFITQ FPRWENQQDL DAEELKVRFQ KFVDETKNNC
360 370 380 390 400
RTMETFCPEC HTVDLKDPVL SDLELWLHAW KYEEINGKFK FKTDLPKWAQ

LDNS
Length:404
Mass (Da):46,816
Last modified:October 1, 1996 - v1
Checksum:iA654F170BA4E33A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72954 Genomic DNA. Translation: CAA97195.1.
BK006941 Genomic DNA. Translation: DAA08263.1.
PIRiS64480.
RefSeqiNP_011685.3. NM_001181298.3.

Genome annotation databases

EnsemblFungiiYGR169C; YGR169C; YGR169C.
GeneIDi853079.
KEGGisce:YGR169C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72954 Genomic DNA. Translation: CAA97195.1.
BK006941 Genomic DNA. Translation: DAA08263.1.
PIRiS64480.
RefSeqiNP_011685.3. NM_001181298.3.

3D structure databases

ProteinModelPortaliP53294.
SMRiP53294. Positions 121-304.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33421. 8 interactions.
DIPiDIP-5429N.
MINTiMINT-544439.

PTM databases

iPTMnetiP53294.

Proteomic databases

MaxQBiP53294.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR169C; YGR169C; YGR169C.
GeneIDi853079.
KEGGisce:YGR169C.

Organism-specific databases

EuPathDBiFungiDB:YGR169C.
SGDiS000003401. PUS6.

Phylogenomic databases

GeneTreeiENSGT00510000048339.
HOGENOMiHOG000167783.
InParanoidiP53294.
KOiK15453.
OMAiCYRLDKI.
OrthoDBiEOG7M0P19.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16704.
YEAST:YGR169C-MONOMER.
BRENDAi5.4.99.42. 984.

Miscellaneous databases

PROiP53294.

Family and domain databases

InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR006224. PsdUridine_synth_RluC/D_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
PROSITEiPS01129. PSI_RLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification and characterization of the tRNA:Psi 31-synthase (Pus6p) of Saccharomyces cerevisiae."
    Ansmant I., Motorin Y., Massenet S., Grosjean H., Branlant C.
    J. Biol. Chem. 276:34934-34940(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, CHARACTERIZATION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-168.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUS6_YEAST
AccessioniPrimary (citable) accession number: P53294
Secondary accession number(s): D6VUV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.