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Protein

tRNA pseudouridine(31) synthase

Gene

PUS6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of pseudouridine at position 31 in the psi GC loop of tRNAS.1 Publication

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

tRNA uridine(31) = tRNA pseudouridine(31).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1681

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: GO_Central
  • tRNA pseudouridine synthesis Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:YGR169C-MONOMER
YEAST:YGR169C-MONOMER
BRENDAi5.4.99.42 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(31) synthase (EC:5.4.99.42)
Alternative name(s):
Pseudouridine synthase 6
Pseudouridylate synthase 6
Uracil hydrolyase
tRNA pseudouridine 31 synthase
Short name:
PSI31 synthase
Gene namesi
Name:PUS6
Ordered Locus Names:YGR169C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR169C
SGDiS000003401 PUS6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168D → A: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001627571 – 404tRNA pseudouridine(31) synthaseAdd BLAST404

Proteomic databases

MaxQBiP53294
PaxDbiP53294
PRIDEiP53294

PTM databases

iPTMnetiP53294

Interactioni

Protein-protein interaction databases

BioGridi33421, 32 interactors
DIPiDIP-5429N
STRINGi4932.YGR169C

Structurei

3D structure databases

ProteinModelPortaliP53294
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase RluA family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000048339
HOGENOMiHOG000167783
InParanoidiP53294
KOiK15453
OMAiPCYRLDK
OrthoDBiEOG092C23UC

Family and domain databases

InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR006224 PsdUridine_synth_RluC/D_CS
IPR006145 PsdUridine_synth_RsuA/RluD
PfamiView protein in Pfam
PF00849 PseudoU_synth_2, 1 hit
SUPFAMiSSF55120 SSF55120, 2 hits
PROSITEiView protein in PROSITE
PS01129 PSI_RLU, 1 hit

Sequencei

Sequence statusi: Complete.

P53294-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIKVIEVY TQNGLRKVRP YYNRRSAFVK GRWLGKLLID VLVSEFKLRP
60 70 80 90 100
RAYYLDQIRK GTYRLIRDGV PLVPDHLMTT IIKNHDVLET TTHKHEPPVK
110 120 130 140 150
QWCSQEVEAE DLPGRIAGFN IVFEDESILV IDKPSGIPVH PTGQFYQNTI
160 170 180 190 200
TELLKLHGVD ALPCYRLDKI TSGLLILAKN SQSAGEIQKS IRSRDMIKIY
210 220 230 240 250
LARVKGRFPH SELILDNENA AETTFEDTSK VTVEMTPIYS IDPKRQFPVG
260 270 280 290 300
LSTSKDAITK FYPIRYFSHA DETVVACKPI TGRTHQIRIH LARLGHPIVN
310 320 330 340 350
DSVYCSHITK YPERLKFITQ FPRWENQQDL DAEELKVRFQ KFVDETKNNC
360 370 380 390 400
RTMETFCPEC HTVDLKDPVL SDLELWLHAW KYEEINGKFK FKTDLPKWAQ

LDNS
Length:404
Mass (Da):46,816
Last modified:October 1, 1996 - v1
Checksum:iA654F170BA4E33A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72954 Genomic DNA Translation: CAA97195.1
BK006941 Genomic DNA Translation: DAA08263.1
PIRiS64480
RefSeqiNP_011685.3, NM_001181298.3

Genome annotation databases

EnsemblFungiiYGR169C; YGR169C; YGR169C
GeneIDi853079
KEGGisce:YGR169C

Similar proteinsi

Entry informationi

Entry nameiPUS6_YEAST
AccessioniPrimary (citable) accession number: P53294
Secondary accession number(s): D6VUV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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