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P53289 (RTS3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase type 2A regulatory subunit RTS3
Gene names
Name:RTS3
Ordered Locus Names:YGR161C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be a component of a protein phosphatase type 2A (PP2A) complex. Negatively regulates SIT4 phosphatase, a modulators of caffeine sensitivity. Ref.7 Ref.8 Ref.11

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 2100 molecules/cell in log phase SD medium.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from direct assay Ref.5. Source: SGD

nucleus

Inferred from direct assay Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Protein phosphatase type 2A regulatory subunit RTS3
PRO_0000202835

Amino acid modifications

Modified residue471Phosphoserine Ref.10
Modified residue491Phosphoserine Ref.10
Modified residue501Phosphoserine Ref.10
Modified residue511Phosphoserine Ref.10
Modified residue1101Phosphoserine Ref.9
Modified residue1111Phosphoserine Ref.10
Modified residue1181Phosphoserine Ref.10
Modified residue1521Phosphothreonine Ref.9 Ref.10
Modified residue1721Phosphoserine Ref.10
Modified residue1921Phosphoserine Ref.9 Ref.10
Modified residue2161Phosphoserine Ref.9 Ref.10
Modified residue2191Phosphoserine Ref.9 Ref.10
Modified residue2201Phosphoserine Ref.9
Modified residue2381Phosphoserine Ref.9 Ref.10
Modified residue2411Phosphoserine Ref.10

Natural variations

Natural variant201V → I. Ref.1
Natural variant2011P → S. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53289 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BA31D32B9ADF02AB

FASTA26329,263
        10         20         30         40         50         60 
MIATSRAVNM NKESKHKKAV AKPCRERQTS VTRAMRPAVA RDPRRLSTSS SPSSSPMSAQ 

        70         80         90        100        110        120 
RRLSREEIIN EMEKEQDAIV VRLLREIETL KEENSRLKNQ LHHPVPARRS SPFFEGESAI 

       130        140        150        160        170        180 
LDDDDCNYGY TLDTPKLKLT DGASRHTVLP LTPKDSMTHI SHSARRSSRN ASISNGTSIS 

       190        200        210        220        230        240 
DTIFPIETKI HSAPTTNRNL PSADLPHHTL LPRSLSGISS SDLTESGALL HDRRRRSSNY 

       250        260 
SLDGSNSLKA DLMAKRFQTG SLK

« Hide

References

« Hide 'large scale' references
[1]"Polymorphism detected in the yeast ORF YGR161C."
Reis V.C.B., Torres F.A.G.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-20 AND SER-201.
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Predicting protein functions from redundancies in large-scale protein interaction networks."
Samanta M.P., Liang S.
Proc. Natl. Acad. Sci. U.S.A. 100:12579-12583(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PREDICTION OF FUNCTION.
[8]"Downregulation of PP2A(Cdc55) phosphatase by separase initiates mitotic exit in budding yeast."
Queralt E., Lehane C., Novak B., Uhlmann F.
Cell 125:719-732(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; THR-152; SER-192; SER-216; SER-219; SER-220 AND SER-238, MASS SPECTROMETRY.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-47; SER-49; SER-50; SER-51; SER-111; SER-118; THR-152; SER-172; SER-192; SER-216; SER-219; SER-238 AND SER-241, MASS SPECTROMETRY.
[11]"Identification of phosphatase 2A-like Sit4-mediated signalling and ubiquitin-dependent protein sorting as modulators of caffeine sensitivity in S. cerevisiae."
Hood-Degrenier J.K.
Yeast 28:189-204(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY301067 Genomic DNA. Translation: AAP48998.1.
Z72946 Genomic DNA. Translation: CAA97175.1.
BK006941 Genomic DNA. Translation: DAA08251.1.
PIRS64472.
RefSeqNP_011677.3. NM_001181290.3.

3D structure databases

ProteinModelPortalP53289.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6319N.
IntActP53289. 13 interactions.
MINTMINT-657378.
STRING4932.YGR161C.

Proteomic databases

PaxDbP53289.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR161C; YGR161C; YGR161C.
GeneID853065.
KEGGsce:YGR161C.
sce:YGR162W.

Organism-specific databases

CYGDYGR161c.
SGDS000003393. RTS3.

Phylogenomic databases

eggNOGNOG85313.
KOK03260.
OrthoDBEOG4MGWJB.

Gene expression databases

GenevestigatorP53289.
GermOnlineYGR161C. Saccharomyces cerevisiae.

Family and domain databases

ProtoNetSearch...

Other

NextBio973007.

Entry information

Entry nameRTS3_YEAST
AccessionPrimary (citable) accession number: P53289
Secondary accession number(s): D6VUU0, Q7ZA12
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents