Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyamine transporter 2

Gene

TPO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine.2 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30843-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.67. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 2
Gene namesi
Name:TPO2
Ordered Locus Names:YGR138C
ORF Names:G6417
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR138C.
SGDiS000003370. TPO2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 173CytoplasmicSequence analysisAdd BLAST173
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 206ExtracellularSequence analysisAdd BLAST12
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 236CytoplasmicSequence analysis9
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 266ExtracellularSequence analysis9
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 297CytoplasmicSequence analysis10
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 326ExtracellularSequence analysis8
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Topological domaini348 – 407CytoplasmicSequence analysisAdd BLAST60
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Topological domaini429 – 437ExtracellularSequence analysis9
Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
Topological domaini459 – 478CytoplasmicSequence analysisAdd BLAST20
Transmembranei479 – 499HelicalSequence analysisAdd BLAST21
Topological domaini500 – 503ExtracellularSequence analysis4
Transmembranei504 – 524HelicalSequence analysisAdd BLAST21
Topological domaini525 – 541CytoplasmicSequence analysisAdd BLAST17
Transmembranei542 – 562HelicalSequence analysisAdd BLAST21
Topological domaini563 – 574ExtracellularSequence analysisAdd BLAST12
Transmembranei575 – 595HelicalSequence analysisAdd BLAST21
Topological domaini596 – 614CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001734391 – 614Polyamine transporter 2Add BLAST614

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53283.
PRIDEiP53283.

PTM databases

iPTMnetiP53283.

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi33385. 42 interactors.
DIPiDIP-7902N.
IntActiP53283. 24 interactors.
MINTiMINT-4487428.

Structurei

3D structure databases

ProteinModelPortaliP53283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiP53283.
OMAiDETICTE.
OrthoDBiEOG092C2079.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQESVVSF NSQNTSMVDV EGQQPQQYVP SKTNSRANQL KLTKTETVKS
60 70 80 90 100
LQDLGVTSAA PVPDINAPQT AKNNIFPEEY TMETPSGLVP VATLQSMGRT
110 120 130 140 150
ASALSRTRTK QLNRTATNSS STGKEEMEEE ETEEREDQSG ENELDPEIEF
160 170 180 190 200
VTFVTGDPEN PHNWPSWVRW SYTVLLSILV ICVAYGSACI SGGLGTVEKK
210 220 230 240 250
YHVGMEAAIL SCSLMVIGFS LGPLIWSPVS DLYGRRVAYF VSMGLYVIFN
260 270 280 290 300
IPCALAPNLG CLLACRFLCG VWSSSGLCLV GGSIADMFPS ETRGKAIAFF
310 320 330 340 350
AFAPYVGPVV GPLVNGFISV STGRMDLIFW VNMAFAGVMW IISSAIPETY
360 370 380 390 400
APVILKRKAA RLRKETGNPK IMTEQEAQGV SMSEMMRACL LRPLYFAVTE
410 420 430 440 450
PVLVATCFYV CLIYSLLYAF FFAFPVIFGE LYGYKDNLVG LMFIPIVIGA
460 470 480 490 500
LWALATTFYC ENKYLQIVKQ RKPTPEDRLL GAKIGAPFAA IALWILGATA
510 520 530 540 550
YKHIIWVGPA SAGLAFGFGM VLIYYSLNNY IIDCYVQYAS SALATKVFLR
560 570 580 590 600
SAGGAAFPLF TIQMYHKLNL HWGSWLLAFI STAMIALPFA FSYWGKGLRH
610
KLSKKDYSID SVEM
Length:614
Mass (Da):67,589
Last modified:October 1, 1996 - v1
Checksum:iC6B9D48720D4F2D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72923 Genomic DNA. Translation: CAA97151.1.
BK006941 Genomic DNA. Translation: DAA08230.1.
PIRiS64447.
RefSeqiNP_011654.1. NM_001181267.1.

Genome annotation databases

EnsemblFungiiYGR138C; YGR138C; YGR138C.
GeneIDi853039.
KEGGisce:YGR138C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72923 Genomic DNA. Translation: CAA97151.1.
BK006941 Genomic DNA. Translation: DAA08230.1.
PIRiS64447.
RefSeqiNP_011654.1. NM_001181267.1.

3D structure databases

ProteinModelPortaliP53283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33385. 42 interactors.
DIPiDIP-7902N.
IntActiP53283. 24 interactors.
MINTiMINT-4487428.

Protein family/group databases

TCDBi2.A.1.2.67. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP53283.

Proteomic databases

MaxQBiP53283.
PRIDEiP53283.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR138C; YGR138C; YGR138C.
GeneIDi853039.
KEGGisce:YGR138C.

Organism-specific databases

EuPathDBiFungiDB:YGR138C.
SGDiS000003370. TPO2.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiP53283.
OMAiDETICTE.
OrthoDBiEOG092C2079.

Enzyme and pathway databases

BioCyciYEAST:G3O-30843-MONOMER.

Miscellaneous databases

PROiP53283.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPO2_YEAST
AccessioniPrimary (citable) accession number: P53283
Secondary accession number(s): D6VUR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.