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P53283

- TPO2_YEAST

UniProt

P53283 - TPO2_YEAST

Protein

Polyamine transporter 2

Gene

TPO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine.2 Publications

    GO - Molecular functioni

    1. antiporter activity Source: UniProtKB-KW
    2. spermine transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. spermine transport Source: SGD

    Keywords - Biological processi

    Antiport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30843-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.2.67. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyamine transporter 2
    Gene namesi
    Name:TPO2
    Ordered Locus Names:YGR138C
    ORF Names:G6417
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR138c.
    SGDiS000003370. TPO2.

    Subcellular locationi

    Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. fungal-type vacuole membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 614614Polyamine transporter 2PRO_0000173439Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53283.
    PaxDbiP53283.

    Expressioni

    Inductioni

    By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

    Gene expression databases

    GenevestigatoriP53283.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-2044753,EBI-2357
    DIP5P533881EBI-2044753,EBI-5904
    DOP1Q039211EBI-2044753,EBI-34442
    FMP45Q076511EBI-2044753,EBI-2051056
    GAP1P191451EBI-2044753,EBI-7314
    GPA2P108231EBI-2044753,EBI-7382
    HNM1P198071EBI-2044753,EBI-8409
    HSP30P256191EBI-2044753,EBI-8563
    HXT1P324651EBI-2044753,EBI-8759
    HXT3P324661EBI-2044753,EBI-8770
    HXT5P386951EBI-2044753,EBI-8778
    HXT7P390041EBI-2044753,EBI-8790
    IST2P382501EBI-2044753,EBI-21520
    MID2P360271EBI-2044753,EBI-10901
    MUP1P502761EBI-2044753,EBI-11624
    PDR12Q027851EBI-2044753,EBI-13065
    PDR5P333021EBI-2044753,EBI-13038
    PHO88P382641EBI-2044753,EBI-13350
    PMP2P409751EBI-2044753,EBI-2043041
    PMP3P872841EBI-2044753,EBI-13555
    RFS1P382341EBI-2044753,EBI-21445
    RIM1P324451EBI-2044753,EBI-15206
    SFK1P357351EBI-2044753,EBI-26678
    SLM1P404851EBI-2044753,EBI-25172
    SNA3P143591EBI-2044753,EBI-26122
    SNQ2P325681EBI-2044753,EBI-17590
    SPF1P399861EBI-2044753,EBI-3128
    TCB3Q036401EBI-2044753,EBI-27872
    TPO1Q078241EBI-2044753,EBI-38106
    TPO3Q064511EBI-2044753,EBI-34275
    YHR146WP388451EBI-2044753,EBI-24770
    YLR326WQ061701EBI-2044753,EBI-35994
    YLR413WQ066891EBI-2044753,EBI-38659

    Protein-protein interaction databases

    BioGridi33385. 43 interactions.
    DIPiDIP-7902N.
    IntActiP53283. 24 interactions.
    MINTiMINT-4487428.
    STRINGi4932.YGR138C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53283.
    SMRiP53283. Positions 307-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 173173CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini195 – 20612ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini228 – 2369CytoplasmicSequence Analysis
    Topological domaini258 – 2669ExtracellularSequence Analysis
    Topological domaini288 – 29710CytoplasmicSequence Analysis
    Topological domaini319 – 3268ExtracellularSequence Analysis
    Topological domaini348 – 40760CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini429 – 4379ExtracellularSequence Analysis
    Topological domaini459 – 47820CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini500 – 5034ExtracellularSequence Analysis
    Topological domaini525 – 54117CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini563 – 57412ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini596 – 61419CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei174 – 19421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei298 – 31821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei327 – 34721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei408 – 42821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei438 – 45821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei479 – 49921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei504 – 52421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei542 – 56221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei575 – 59521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00620000088227.
    HOGENOMiHOG000160689.
    OMAiSANNYLI.
    OrthoDBiEOG77M8XK.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53283-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDQESVVSF NSQNTSMVDV EGQQPQQYVP SKTNSRANQL KLTKTETVKS    50
    LQDLGVTSAA PVPDINAPQT AKNNIFPEEY TMETPSGLVP VATLQSMGRT 100
    ASALSRTRTK QLNRTATNSS STGKEEMEEE ETEEREDQSG ENELDPEIEF 150
    VTFVTGDPEN PHNWPSWVRW SYTVLLSILV ICVAYGSACI SGGLGTVEKK 200
    YHVGMEAAIL SCSLMVIGFS LGPLIWSPVS DLYGRRVAYF VSMGLYVIFN 250
    IPCALAPNLG CLLACRFLCG VWSSSGLCLV GGSIADMFPS ETRGKAIAFF 300
    AFAPYVGPVV GPLVNGFISV STGRMDLIFW VNMAFAGVMW IISSAIPETY 350
    APVILKRKAA RLRKETGNPK IMTEQEAQGV SMSEMMRACL LRPLYFAVTE 400
    PVLVATCFYV CLIYSLLYAF FFAFPVIFGE LYGYKDNLVG LMFIPIVIGA 450
    LWALATTFYC ENKYLQIVKQ RKPTPEDRLL GAKIGAPFAA IALWILGATA 500
    YKHIIWVGPA SAGLAFGFGM VLIYYSLNNY IIDCYVQYAS SALATKVFLR 550
    SAGGAAFPLF TIQMYHKLNL HWGSWLLAFI STAMIALPFA FSYWGKGLRH 600
    KLSKKDYSID SVEM 614
    Length:614
    Mass (Da):67,589
    Last modified:October 1, 1996 - v1
    Checksum:iC6B9D48720D4F2D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72923 Genomic DNA. Translation: CAA97151.1.
    BK006941 Genomic DNA. Translation: DAA08230.1.
    PIRiS64447.
    RefSeqiNP_011654.1. NM_001181267.1.

    Genome annotation databases

    EnsemblFungiiYGR138C; YGR138C; YGR138C.
    GeneIDi853039.
    KEGGisce:YGR138C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72923 Genomic DNA. Translation: CAA97151.1 .
    BK006941 Genomic DNA. Translation: DAA08230.1 .
    PIRi S64447.
    RefSeqi NP_011654.1. NM_001181267.1.

    3D structure databases

    ProteinModelPortali P53283.
    SMRi P53283. Positions 307-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33385. 43 interactions.
    DIPi DIP-7902N.
    IntActi P53283. 24 interactions.
    MINTi MINT-4487428.
    STRINGi 4932.YGR138C.

    Protein family/group databases

    TCDBi 2.A.1.2.67. the major facilitator superfamily (mfs).

    Proteomic databases

    MaxQBi P53283.
    PaxDbi P53283.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR138C ; YGR138C ; YGR138C .
    GeneIDi 853039.
    KEGGi sce:YGR138C.

    Organism-specific databases

    CYGDi YGR138c.
    SGDi S000003370. TPO2.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00620000088227.
    HOGENOMi HOG000160689.
    OMAi SANNYLI.
    OrthoDBi EOG77M8XK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30843-MONOMER.

    Miscellaneous databases

    NextBioi 972941.

    Gene expression databases

    Genevestigatori P53283.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Multiple polyamine transport systems on the vacuolar membrane in yeast."
      Tomitori H., Kashiwagi K., Asakawa T., Kakinuma Y., Michael A.J., Igarashi K.
      Biochem. J. 353:681-688(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Localization and function of the yeast multidrug transporter Tpo1p."
      Albertsen M., Bellahn I., Kraemer R., Waffenschmidt S.
      J. Biol. Chem. 278:12820-12825(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. "Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively."
      Aranda A., del Olmo M.
      Appl. Environ. Microbiol. 70:1913-1922(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTPO2_YEAST
    AccessioniPrimary (citable) accession number: P53283
    Secondary accession number(s): D6VUR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3