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P53283

- TPO2_YEAST

UniProt

P53283 - TPO2_YEAST

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Protein
Polyamine transporter 2
Gene
TPO2, YGR138C, G6417
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine.2 Publications

GO - Molecular functioni

  1. antiporter activity Source: UniProtKB-KW
  2. spermine transmembrane transporter activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30843-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.67. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 2
Gene namesi
Name:TPO2
Ordered Locus Names:YGR138C
ORF Names:G6417
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR138c.
SGDiS000003370. TPO2.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 173173Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei174 – 19421Helical; Reviewed prediction
Add
BLAST
Topological domaini195 – 20612Extracellular Reviewed prediction
Add
BLAST
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Topological domaini228 – 2369Cytoplasmic Reviewed prediction
Transmembranei237 – 25721Helical; Reviewed prediction
Add
BLAST
Topological domaini258 – 2669Extracellular Reviewed prediction
Transmembranei267 – 28721Helical; Reviewed prediction
Add
BLAST
Topological domaini288 – 29710Cytoplasmic Reviewed prediction
Transmembranei298 – 31821Helical; Reviewed prediction
Add
BLAST
Topological domaini319 – 3268Extracellular Reviewed prediction
Transmembranei327 – 34721Helical; Reviewed prediction
Add
BLAST
Topological domaini348 – 40760Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei408 – 42821Helical; Reviewed prediction
Add
BLAST
Topological domaini429 – 4379Extracellular Reviewed prediction
Transmembranei438 – 45821Helical; Reviewed prediction
Add
BLAST
Topological domaini459 – 47820Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei479 – 49921Helical; Reviewed prediction
Add
BLAST
Topological domaini500 – 5034Extracellular Reviewed prediction
Transmembranei504 – 52421Helical; Reviewed prediction
Add
BLAST
Topological domaini525 – 54117Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei542 – 56221Helical; Reviewed prediction
Add
BLAST
Topological domaini563 – 57412Extracellular Reviewed prediction
Add
BLAST
Transmembranei575 – 59521Helical; Reviewed prediction
Add
BLAST
Topological domaini596 – 61419Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Polyamine transporter 2
PRO_0000173439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53283.
PaxDbiP53283.

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Gene expression databases

GenevestigatoriP53283.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-2044753,EBI-2357
DIP5P533881EBI-2044753,EBI-5904
DOP1Q039211EBI-2044753,EBI-34442
FMP45Q076511EBI-2044753,EBI-2051056
GAP1P191451EBI-2044753,EBI-7314
GPA2P108231EBI-2044753,EBI-7382
HNM1P198071EBI-2044753,EBI-8409
HSP30P256191EBI-2044753,EBI-8563
HXT1P324651EBI-2044753,EBI-8759
HXT3P324661EBI-2044753,EBI-8770
HXT5P386951EBI-2044753,EBI-8778
HXT7P390041EBI-2044753,EBI-8790
IST2P382501EBI-2044753,EBI-21520
MID2P360271EBI-2044753,EBI-10901
MUP1P502761EBI-2044753,EBI-11624
PDR12Q027851EBI-2044753,EBI-13065
PDR5P333021EBI-2044753,EBI-13038
PHO88P382641EBI-2044753,EBI-13350
PMP2P409751EBI-2044753,EBI-2043041
PMP3P872841EBI-2044753,EBI-13555
RFS1P382341EBI-2044753,EBI-21445
RIM1P324451EBI-2044753,EBI-15206
SFK1P357351EBI-2044753,EBI-26678
SLM1P404851EBI-2044753,EBI-25172
SNA3P143591EBI-2044753,EBI-26122
SNQ2P325681EBI-2044753,EBI-17590
SPF1P399861EBI-2044753,EBI-3128
TCB3Q036401EBI-2044753,EBI-27872
TPO1Q078241EBI-2044753,EBI-38106
TPO3Q064511EBI-2044753,EBI-34275
YHR146WP388451EBI-2044753,EBI-24770
YLR326WQ061701EBI-2044753,EBI-35994
YLR413WQ066891EBI-2044753,EBI-38659

Protein-protein interaction databases

BioGridi33385. 43 interactions.
DIPiDIP-7902N.
IntActiP53283. 24 interactions.
MINTiMINT-4487428.
STRINGi4932.YGR138C.

Structurei

3D structure databases

ProteinModelPortaliP53283.
SMRiP53283. Positions 307-334.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
OMAiSANNYLI.
OrthoDBiEOG77M8XK.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53283-1 [UniParc]FASTAAdd to Basket

« Hide

MSDQESVVSF NSQNTSMVDV EGQQPQQYVP SKTNSRANQL KLTKTETVKS    50
LQDLGVTSAA PVPDINAPQT AKNNIFPEEY TMETPSGLVP VATLQSMGRT 100
ASALSRTRTK QLNRTATNSS STGKEEMEEE ETEEREDQSG ENELDPEIEF 150
VTFVTGDPEN PHNWPSWVRW SYTVLLSILV ICVAYGSACI SGGLGTVEKK 200
YHVGMEAAIL SCSLMVIGFS LGPLIWSPVS DLYGRRVAYF VSMGLYVIFN 250
IPCALAPNLG CLLACRFLCG VWSSSGLCLV GGSIADMFPS ETRGKAIAFF 300
AFAPYVGPVV GPLVNGFISV STGRMDLIFW VNMAFAGVMW IISSAIPETY 350
APVILKRKAA RLRKETGNPK IMTEQEAQGV SMSEMMRACL LRPLYFAVTE 400
PVLVATCFYV CLIYSLLYAF FFAFPVIFGE LYGYKDNLVG LMFIPIVIGA 450
LWALATTFYC ENKYLQIVKQ RKPTPEDRLL GAKIGAPFAA IALWILGATA 500
YKHIIWVGPA SAGLAFGFGM VLIYYSLNNY IIDCYVQYAS SALATKVFLR 550
SAGGAAFPLF TIQMYHKLNL HWGSWLLAFI STAMIALPFA FSYWGKGLRH 600
KLSKKDYSID SVEM 614
Length:614
Mass (Da):67,589
Last modified:October 1, 1996 - v1
Checksum:iC6B9D48720D4F2D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72923 Genomic DNA. Translation: CAA97151.1.
BK006941 Genomic DNA. Translation: DAA08230.1.
PIRiS64447.
RefSeqiNP_011654.1. NM_001181267.1.

Genome annotation databases

EnsemblFungiiYGR138C; YGR138C; YGR138C.
GeneIDi853039.
KEGGisce:YGR138C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72923 Genomic DNA. Translation: CAA97151.1 .
BK006941 Genomic DNA. Translation: DAA08230.1 .
PIRi S64447.
RefSeqi NP_011654.1. NM_001181267.1.

3D structure databases

ProteinModelPortali P53283.
SMRi P53283. Positions 307-334.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33385. 43 interactions.
DIPi DIP-7902N.
IntActi P53283. 24 interactions.
MINTi MINT-4487428.
STRINGi 4932.YGR138C.

Protein family/group databases

TCDBi 2.A.1.2.67. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBi P53283.
PaxDbi P53283.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR138C ; YGR138C ; YGR138C .
GeneIDi 853039.
KEGGi sce:YGR138C.

Organism-specific databases

CYGDi YGR138c.
SGDi S000003370. TPO2.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00620000088227.
HOGENOMi HOG000160689.
OMAi SANNYLI.
OrthoDBi EOG77M8XK.

Enzyme and pathway databases

BioCyci YEAST:G3O-30843-MONOMER.

Miscellaneous databases

NextBioi 972941.

Gene expression databases

Genevestigatori P53283.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Multiple polyamine transport systems on the vacuolar membrane in yeast."
    Tomitori H., Kashiwagi K., Asakawa T., Kakinuma Y., Michael A.J., Igarashi K.
    Biochem. J. 353:681-688(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Localization and function of the yeast multidrug transporter Tpo1p."
    Albertsen M., Bellahn I., Kraemer R., Waffenschmidt S.
    J. Biol. Chem. 278:12820-12825(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. "Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively."
    Aranda A., del Olmo M.
    Appl. Environ. Microbiol. 70:1913-1922(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPO2_YEAST
AccessioniPrimary (citable) accession number: P53283
Secondary accession number(s): D6VUR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi