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Protein

LAS seventeen-binding protein 1

Gene

LSB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in resistance to EDTA.1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

BioCyciYEAST:G3O-30842-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
LAS seventeen-binding protein 1
Short name:
LAS17-binding protein 1
Gene namesi
Name:LSB1
Ordered Locus Names:YGR136W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR136W.
SGDiS000003368. LSB1.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
  • ruffle membrane Source: GO_Central

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90W → S: Abolishes interaction with LAS17, but not with SUP35. Blocks colocalization with actin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002028252 – 241LAS seventeen-binding protein 1Add BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Cross-linki41Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources2 Publications
Modified residuei48PhosphoserineCombined sources1
Cross-linki79Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources2 Publications
Modified residuei114PhosphoserineCombined sources1
Modified residuei116PhosphoserineCombined sources1
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

Ubiquitinated by RSP5.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP53281.
PRIDEiP53281.

PTM databases

iPTMnetiP53281.

Interactioni

Subunit structurei

Interacts with LAS17, RSP5 and SUP35.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33384. 43 interactors.
DIPiDIP-1863N.
IntActiP53281. 29 interactors.
MINTiMINT-375073.
STRINGi4932.YGR136W.

Structurei

3D structure databases

ProteinModelPortaliP53281.
SMRiP53281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 112SH3PROSITE-ProRule annotationAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi135 – 138PY motif4

Domaini

The PY motif is recognized directly by the WW domains of RSP5.By similarity

Sequence similaritiesi

Belongs to the LSB1 family.Curated

Keywords - Domaini

SH3 domain

Phylogenomic databases

GeneTreeiENSGT00510000054224.
HOGENOMiHOG000195703.
InParanoidiP53281.
KOiK20522.
OMAiIQIVEYV.
OrthoDBiEOG092C454D.

Family and domain databases

InterProiView protein in InterPro
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF00018. SH3_1. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
SMARTiView protein in SMART
SM00326. SH3. 1 hit.
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS50002. SH3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53281-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASLVNRSL KNIRNELEFL KESNVISGDI FELINSKLPE KWDGNQRSPQ
60 70 80 90 100
NADTEEYVEA LYDFEAQQDG DLSLKTGDKI QVLEKISPDW YRGKSNNKIG
110 120 130 140 150
IFPANYVKPA FTRSASPKSA EAASSSTVSR PSVPPPSYEP AASQYPSQQV
160 170 180 190 200
SAPYAPPAGY MQAPPPQQQQ APLPYPPPFT NYYQQPQQQY APPSQQAPVE
210 220 230 240
AQPQQSSGAS SAFKSFGSKL GNAAIFGAGS AIGSDIVNSI F
Length:241
Mass (Da):26,139
Last modified:October 1, 1996 - v1
Checksum:i5F0B1361AF84AA79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72921 Genomic DNA. Translation: CAA97149.1.
BK006941 Genomic DNA. Translation: DAA08229.1.
PIRiS64445.
RefSeqiNP_011652.1. NM_001181265.1.

Genome annotation databases

EnsemblFungiiYGR136W; YGR136W; YGR136W.
GeneIDi853037.
KEGGisce:YGR136W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLSB1_YEAST
AccessioniPrimary (citable) accession number: P53281
Secondary accession number(s): D6VUR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names