ID   CF130_YEAST             Reviewed;        1122 AA.
AC   P53280; D6VUR6;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 165.
DE   RecName: Full=Protein CAF130;
DE   AltName: Full=130 kDa CCR4-associated factor;
GN   Name=CAF130; OrderedLocusNames=YGR134W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, AND INTERACTION WITH NOT1.
RX   PubMed=11733989; DOI=10.1006/jmbi.2001.5162;
RA   Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.;
RT   "Purification and characterization of the 1.0 MDa CCR4-NOT complex
RT   identifies two novel components of the complex.";
RL   J. Mol. Biol. 314:683-694(2001).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1042, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC   -!- FUNCTION: Acts as a component of the CCR4-NOT core complex, which in
CC       the nucleus seems to be a general transcription factor, and in the
CC       cytoplasm the major mRNA deadenylase involved in mRNA turnover.
CC   -!- SUBUNIT: Subunit of the 1.0 MDa CCR4-NOT core complex that contains
CC       CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the
CC       complex interacts with NOT1. The core complex probably is part of a
CC       less characterized 1.9 MDa CCR4-NOT complex.
CC       {ECO:0000269|PubMed:11733989}.
CC   -!- INTERACTION:
CC       P53280; P29366: BEM1; NbExp=3; IntAct=EBI-23322, EBI-3508;
CC       P53280; P25655: CDC39; NbExp=6; IntAct=EBI-23322, EBI-12139;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Nucleus {ECO:0000305}.
CC   -!- MISCELLANEOUS: Present with 1320 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z72919; CAA97147.1; -; Genomic_DNA.
DR   EMBL; AY558112; AAS56438.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08227.1; -; Genomic_DNA.
DR   PIR; S64443; S64443.
DR   RefSeq; NP_011650.3; NM_001181263.3.
DR   AlphaFoldDB; P53280; -.
DR   BioGRID; 33382; 165.
DR   ComplexPortal; CPX-1800; CCR4-NOT mRNA deadenylase complex.
DR   DIP; DIP-2683N; -.
DR   IntAct; P53280; 9.
DR   MINT; P53280; -.
DR   STRING; 4932.YGR134W; -.
DR   iPTMnet; P53280; -.
DR   MaxQB; P53280; -.
DR   PaxDb; 4932-YGR134W; -.
DR   PeptideAtlas; P53280; -.
DR   TopDownProteomics; P53280; -.
DR   ABCD; P53280; 7 sequenced antibodies.
DR   EnsemblFungi; YGR134W_mRNA; YGR134W; YGR134W.
DR   GeneID; 853035; -.
DR   KEGG; sce:YGR134W; -.
DR   AGR; SGD:S000003366; -.
DR   SGD; S000003366; CAF130.
DR   VEuPathDB; FungiDB:YGR134W; -.
DR   eggNOG; ENOG502QT7N; Eukaryota.
DR   HOGENOM; CLU_280757_0_0_1; -.
DR   InParanoid; P53280; -.
DR   OMA; EMLMCSG; -.
DR   OrthoDB; 1977706at2759; -.
DR   BioCyc; YEAST:G3O-30840-MONOMER; -.
DR   BioGRID-ORCS; 853035; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P53280; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53280; Protein.
DR   GO; GO:0030015; C:CCR4-NOT core complex; IPI:SGD.
DR   GO; GO:0005829; C:cytosol; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; NAS:ComplexPortal.
DR   GO; GO:0032968; P:positive regulation of transcription elongation by RNA polymerase II; IDA:SGD.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..1122
FT                   /note="Protein CAF130"
FT                   /id="PRO_0000202824"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        7..22
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1042
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
SQ   SEQUENCE   1122 AA;  128780 MW;  BF3542816CC78490 CRC64;
     MTKKKAATNY AERQNLASED SSGDSVHFKD FIPLQELLKD KNYVPSVENL EKILYNETMF
     NDQKICSNLL LEALIITLFT TISGKSALRL IQTSSLKERK SWAQSFENNS SSYASIVLSW
     KDNDILLLKF LRFLLANKTA PLQINRYNLP EYKLPLSFLI VSKITIPSIL LNETYNLLKD
     YLYSITGRIE SLISCSSTFD KPALVVRKIL KDYNRMIECR NFYFWYSFNA ENRVNLTFSD
     NISLLMENDE GNAGSGLDDS RFDHQKQPRE AIMGRTINDQ EQIYSFELNQ DGTLEIPNVM
     EHSLLRHELL FKILNLTTVL TPLLELQFST LCGLVDPLMQ PTPNDKHIIS IDFLFQLFLG
     LMSQSIKTSQ EHNDHYDWKF YMCFNMQKII DATMLRLNCF DFDILNSVNN TDNAVHWKTQ
     LHRWLPHGLN TQDLELLYMI DILAVYTIYK LYEKIPIQLN PFLFSLISLW KNLSCVILLA
     LEIDRIEEEN GTYETPLMVR ATIRGAAALR SVIATVLNGL VKNNDHDFKH ESLNTFMSPY
     GRKLCHGALY ADLRSHTASL LALGASIEDV TDLFADLQSG DRFDEDIRYM FDYECEDYDE
     SFSESDHGGL DESVVNPTEK IASGSNNVFF RRRCNCIFND DKLVAEDGAN EAFGSTNSEN
     VEGAMHNNRN AVHNATTATS DHVVTSPNPL SVRSRSTFEF DYSGEDWRDV PRDFNMYYSP
     SYSFIHEPKL DVIFSLTLRG ATEKLNKEES ILLVRSVASC VRNEQDQMIL ADLESNFSAS
     INGDVEGEGN TKMSKIDNED LRRTTPDDIY EIWSEESAFE RMLNVNHDVA WRLMDEMLMC
     TGYRRILIWF LTHLELKHSL IYYVFELIMG LRGKPFSGEA SDQDKKDDMI YEILKKKQKN
     EDASGLPFSR QGPIVLSDIE TKMLLQEFFM NAAIFLSSKN NEEENEDGEK ISLYSLGLVR
     LICYMVQTLI ANDKFFFTKS ECTFELQTLL MTWIGILPEA KDLFFKIKTR LAMEEEDSAD
     TMQHEGRKNS DIEKKLNAKP ASELNLKLLN LFPSKPANKD DSSPINTLRS FIADYSFDTQ
     VNPPGRRVVF YDGKILPLPK ADKPIPLHEY ITLAELDVGD SE
//