P53280 (CF130_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein CAF130

Alternative name(s):
130 kDa CCR4-associated factor

Gene names

Name:	CAF130
Ordered Locus Names:	YGR134W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1122 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover.
Subunit structure	Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.4
Subcellular location	Cytoplasm Probable. Nucleus Probable.
Miscellaneous	Present with 1320 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription elongation from RNA polymerase II promoter Inferred from direct assay PubMed 21406554. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	CCR4-NOT core complex Inferred from physical interaction Ref.4. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BEM1	P29366	3	EBI-23322,EBI-3508
CDC39	P25655	6	EBI-23322,EBI-12139

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1122	1122	Protein CAF130		PRO_0000202824
Amino acid modifications
	Modified residue	623	1	Phosphoserine Ref.6
	Modified residue	625	1	Phosphoserine Ref.6
	Modified residue	685	1	Phosphothreonine Ref.6
	Modified residue	686	1	Phosphoserine Ref.6
	Modified residue	1042	1	Phosphoserine Ref.6
	Modified residue	1063	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P53280 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BF3542816CC78490	FASTA	1,122	128,780
	10 20 30 40 50 60 MTKKKAATNY AERQNLASED SSGDSVHFKD FIPLQELLKD KNYVPSVENL EKILYNETMF 70 80 90 100 110 120 NDQKICSNLL LEALIITLFT TISGKSALRL IQTSSLKERK SWAQSFENNS SSYASIVLSW 130 140 150 160 170 180 KDNDILLLKF LRFLLANKTA PLQINRYNLP EYKLPLSFLI VSKITIPSIL LNETYNLLKD 190 200 210 220 230 240 YLYSITGRIE SLISCSSTFD KPALVVRKIL KDYNRMIECR NFYFWYSFNA ENRVNLTFSD 250 260 270 280 290 300 NISLLMENDE GNAGSGLDDS RFDHQKQPRE AIMGRTINDQ EQIYSFELNQ DGTLEIPNVM 310 320 330 340 350 360 EHSLLRHELL FKILNLTTVL TPLLELQFST LCGLVDPLMQ PTPNDKHIIS IDFLFQLFLG 370 380 390 400 410 420 LMSQSIKTSQ EHNDHYDWKF YMCFNMQKII DATMLRLNCF DFDILNSVNN TDNAVHWKTQ 430 440 450 460 470 480 LHRWLPHGLN TQDLELLYMI DILAVYTIYK LYEKIPIQLN PFLFSLISLW KNLSCVILLA 490 500 510 520 530 540 LEIDRIEEEN GTYETPLMVR ATIRGAAALR SVIATVLNGL VKNNDHDFKH ESLNTFMSPY 550 560 570 580 590 600 GRKLCHGALY ADLRSHTASL LALGASIEDV TDLFADLQSG DRFDEDIRYM FDYECEDYDE 610 620 630 640 650 660 SFSESDHGGL DESVVNPTEK IASGSNNVFF RRRCNCIFND DKLVAEDGAN EAFGSTNSEN 670 680 690 700 710 720 VEGAMHNNRN AVHNATTATS DHVVTSPNPL SVRSRSTFEF DYSGEDWRDV PRDFNMYYSP 730 740 750 760 770 780 SYSFIHEPKL DVIFSLTLRG ATEKLNKEES ILLVRSVASC VRNEQDQMIL ADLESNFSAS 790 800 810 820 830 840 INGDVEGEGN TKMSKIDNED LRRTTPDDIY EIWSEESAFE RMLNVNHDVA WRLMDEMLMC 850 860 870 880 890 900 TGYRRILIWF LTHLELKHSL IYYVFELIMG LRGKPFSGEA SDQDKKDDMI YEILKKKQKN 910 920 930 940 950 960 EDASGLPFSR QGPIVLSDIE TKMLLQEFFM NAAIFLSSKN NEEENEDGEK ISLYSLGLVR 970 980 990 1000 1010 1020 LICYMVQTLI ANDKFFFTKS ECTFELQTLL MTWIGILPEA KDLFFKIKTR LAMEEEDSAD 1030 1040 1050 1060 1070 1080 TMQHEGRKNS DIEKKLNAKP ASELNLKLLN LFPSKPANKD DSSPINTLRS FIADYSFDTQ 1090 1100 1110 1120 VNPPGRRVVF YDGKILPLPK ADKPIPLHEY ITLAELDVGD SE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex." Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L. J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, INTERACTION WITH NOT1.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623; SER-625; THR-685; SER-686; SER-1042 AND SER-1063, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72919 Genomic DNA. Translation: CAA97147.1. AY558112 Genomic DNA. Translation: AAS56438.1. BK006941 Genomic DNA. Translation: DAA08227.1.
PIR	S64443.
RefSeq	NP_011650.3. NM_001181263.3.
3D structure databases
ProteinModelPortal	P53280.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2683N.
IntAct	P53280. 9 interactions.
MINT	MINT-543779.
STRING	4932.YGR134W.
Proteomic databases
PaxDb	P53280.
PeptideAtlas	P53280.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR134W; YGR134W; YGR134W.
GeneID	853035.
KEGG	sce:YGR134W.
Organism-specific databases
CYGD	YGR134w.
SGD	S000003366. CAF130.
Phylogenomic databases
eggNOG	NOG39522.
HOGENOM	HOG000111568.
OMA	FDEDIRY.
OrthoDB	EOG4X6GHJ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30840-MONOMER.
Gene expression databases
Genevestigator	P53280.
GermOnline	YGR134W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	972932.

Entry information

Entry name

CF130_YEAST

Accession

Primary (citable) accession number: P53280
Secondary accession number(s): D6VUR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program