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P53280 (CF130_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein CAF130
Alternative name(s):
130 kDa CCR4-associated factor
Gene names
Name:CAF130
Ordered Locus Names:YGR134W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1122 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover.

Subunit structure

Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.4

Subcellular location

Cytoplasm Probable. Nucleus Probable.

Miscellaneous

Present with 1320 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BEM1P293663EBI-23322,EBI-3508
CDC39P256556EBI-23322,EBI-12139

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11221122Protein CAF130
PRO_0000202824

Amino acid modifications

Modified residue10421Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P53280 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BF3542816CC78490

FASTA1,122128,780
        10         20         30         40         50         60 
MTKKKAATNY AERQNLASED SSGDSVHFKD FIPLQELLKD KNYVPSVENL EKILYNETMF 

        70         80         90        100        110        120 
NDQKICSNLL LEALIITLFT TISGKSALRL IQTSSLKERK SWAQSFENNS SSYASIVLSW 

       130        140        150        160        170        180 
KDNDILLLKF LRFLLANKTA PLQINRYNLP EYKLPLSFLI VSKITIPSIL LNETYNLLKD 

       190        200        210        220        230        240 
YLYSITGRIE SLISCSSTFD KPALVVRKIL KDYNRMIECR NFYFWYSFNA ENRVNLTFSD 

       250        260        270        280        290        300 
NISLLMENDE GNAGSGLDDS RFDHQKQPRE AIMGRTINDQ EQIYSFELNQ DGTLEIPNVM 

       310        320        330        340        350        360 
EHSLLRHELL FKILNLTTVL TPLLELQFST LCGLVDPLMQ PTPNDKHIIS IDFLFQLFLG 

       370        380        390        400        410        420 
LMSQSIKTSQ EHNDHYDWKF YMCFNMQKII DATMLRLNCF DFDILNSVNN TDNAVHWKTQ 

       430        440        450        460        470        480 
LHRWLPHGLN TQDLELLYMI DILAVYTIYK LYEKIPIQLN PFLFSLISLW KNLSCVILLA 

       490        500        510        520        530        540 
LEIDRIEEEN GTYETPLMVR ATIRGAAALR SVIATVLNGL VKNNDHDFKH ESLNTFMSPY 

       550        560        570        580        590        600 
GRKLCHGALY ADLRSHTASL LALGASIEDV TDLFADLQSG DRFDEDIRYM FDYECEDYDE 

       610        620        630        640        650        660 
SFSESDHGGL DESVVNPTEK IASGSNNVFF RRRCNCIFND DKLVAEDGAN EAFGSTNSEN 

       670        680        690        700        710        720 
VEGAMHNNRN AVHNATTATS DHVVTSPNPL SVRSRSTFEF DYSGEDWRDV PRDFNMYYSP 

       730        740        750        760        770        780 
SYSFIHEPKL DVIFSLTLRG ATEKLNKEES ILLVRSVASC VRNEQDQMIL ADLESNFSAS 

       790        800        810        820        830        840 
INGDVEGEGN TKMSKIDNED LRRTTPDDIY EIWSEESAFE RMLNVNHDVA WRLMDEMLMC 

       850        860        870        880        890        900 
TGYRRILIWF LTHLELKHSL IYYVFELIMG LRGKPFSGEA SDQDKKDDMI YEILKKKQKN 

       910        920        930        940        950        960 
EDASGLPFSR QGPIVLSDIE TKMLLQEFFM NAAIFLSSKN NEEENEDGEK ISLYSLGLVR 

       970        980        990       1000       1010       1020 
LICYMVQTLI ANDKFFFTKS ECTFELQTLL MTWIGILPEA KDLFFKIKTR LAMEEEDSAD 

      1030       1040       1050       1060       1070       1080 
TMQHEGRKNS DIEKKLNAKP ASELNLKLLN LFPSKPANKD DSSPINTLRS FIADYSFDTQ 

      1090       1100       1110       1120 
VNPPGRRVVF YDGKILPLPK ADKPIPLHEY ITLAELDVGD SE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, INTERACTION WITH NOT1.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1042, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72919 Genomic DNA. Translation: CAA97147.1.
AY558112 Genomic DNA. Translation: AAS56438.1.
BK006941 Genomic DNA. Translation: DAA08227.1.
PIRS64443.
RefSeqNP_011650.3. NM_001181263.3.

3D structure databases

ProteinModelPortalP53280.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33382. 61 interactions.
DIPDIP-2683N.
IntActP53280. 9 interactions.
MINTMINT-543779.
STRING4932.YGR134W.

Proteomic databases

MaxQBP53280.
PaxDbP53280.
PeptideAtlasP53280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR134W; YGR134W; YGR134W.
GeneID853035.
KEGGsce:YGR134W.

Organism-specific databases

CYGDYGR134w.
SGDS000003366. CAF130.

Phylogenomic databases

eggNOGNOG39522.
HOGENOMHOG000111568.
OMALWKCHTN.
OrthoDBEOG7NSB9P.

Enzyme and pathway databases

BioCycYEAST:G3O-30840-MONOMER.

Gene expression databases

GenevestigatorP53280.

Family and domain databases

ProtoNetSearch...

Other

NextBio972932.

Entry information

Entry nameCF130_YEAST
AccessionPrimary (citable) accession number: P53280
Secondary accession number(s): D6VUR6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD