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Protein

Pre-mRNA-splicing factor SYF2

Gene

SYF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing and cell cycle control. As a component of the NTC complex (or PRP19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. The cell cycle arrest of SYF2 defective cells may be due to the inefficient splicing of TUB1.3 Publications

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30835-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SYF2
Alternative name(s):
PRP19 complex protein 31
Synthetic lethal with CDC40 protein 2
Gene namesi
Name:SYF2
Synonyms:NTC31
Ordered Locus Names:YGR129W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR129W.
SGDiS000003361. SYF2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • Prp19 complex Source: SGD
  • U2-type catalytic step 1 spliceosome Source: SGD
  • U2-type catalytic step 2 spliceosome Source: SGD
  • U2-type post-mRNA release spliceosomal complex Source: SGD
  • U2-type prespliceosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000723811 – 215Pre-mRNA-splicing factor SYF2Add BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphoserineCombined sources1
Modified residuei124PhosphoserineCombined sources1
Modified residuei125PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53277.
PRIDEiP53277.

PTM databases

iPTMnetiP53277.

Interactioni

Subunit structurei

Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CEF1, CLF1 and SYF1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CEF1Q036543EBI-23308,EBI-476
CLF1Q123092EBI-23308,EBI-484
NTC20P383022EBI-23308,EBI-20921
PRP19P325234EBI-23308,EBI-493
SYF1Q040484EBI-23308,EBI-540

Protein-protein interaction databases

BioGridi33377. 55 interactors.
DIPiDIP-1684N.
IntActiP53277. 14 interactors.
MINTiMINT-402831.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50f1-215[»]
5GMKelectron microscopy3.40I1-215[»]
ProteinModelPortaliP53277.
SMRiP53277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SYF2 family.Curated

Phylogenomic databases

HOGENOMiHOG000000933.
InParanoidiP53277.
KOiK12868.
OMAiVERDIAM.
OrthoDBiEOG092C4OA3.

Family and domain databases

InterProiIPR013260. mRNA_splic_SYF2.
[Graphical view]
PfamiPF08231. SYF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN
60 70 80 90 100
DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQS QRGGISYDQL
110 120 130 140 150
AKLSYEKTLR NLATQTQNSS KQDSSADEED NKNVPKKGRI GKVQKDTKTG
160 170 180 190 200
KITIADDDKL VNKLAVSLQS ESKKRYEARK RQMQNAKTLY GVESFINDKN
210
KQFNEKLSRE SKGSE
Length:215
Mass (Da):24,803
Last modified:October 1, 1996 - v1
Checksum:iC80B872FCD808E1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72914 Genomic DNA. Translation: CAA97142.1.
AY558326 Genomic DNA. Translation: AAS56652.1.
BK006941 Genomic DNA. Translation: DAA08222.1.
PIRiS64438.
RefSeqiNP_011645.3. NM_001181258.3.

Genome annotation databases

EnsemblFungiiYGR129W; YGR129W; YGR129W.
GeneIDi853030.
KEGGisce:YGR129W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72914 Genomic DNA. Translation: CAA97142.1.
AY558326 Genomic DNA. Translation: AAS56652.1.
BK006941 Genomic DNA. Translation: DAA08222.1.
PIRiS64438.
RefSeqiNP_011645.3. NM_001181258.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50f1-215[»]
5GMKelectron microscopy3.40I1-215[»]
ProteinModelPortaliP53277.
SMRiP53277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33377. 55 interactors.
DIPiDIP-1684N.
IntActiP53277. 14 interactors.
MINTiMINT-402831.

PTM databases

iPTMnetiP53277.

Proteomic databases

MaxQBiP53277.
PRIDEiP53277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR129W; YGR129W; YGR129W.
GeneIDi853030.
KEGGisce:YGR129W.

Organism-specific databases

EuPathDBiFungiDB:YGR129W.
SGDiS000003361. SYF2.

Phylogenomic databases

HOGENOMiHOG000000933.
InParanoidiP53277.
KOiK12868.
OMAiVERDIAM.
OrthoDBiEOG092C4OA3.

Enzyme and pathway databases

BioCyciYEAST:G3O-30835-MONOMER.

Miscellaneous databases

PROiP53277.

Family and domain databases

InterProiIPR013260. mRNA_splic_SYF2.
[Graphical view]
PfamiPF08231. SYF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYF2_YEAST
AccessioniPrimary (citable) accession number: P53277
Secondary accession number(s): D6VUR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.