Pre-mRNA-splicing factor SYF2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53277 (SYF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor SYF2

Alternative name(s):
PRP19 complex protein 31
Synthetic lethal with CDC40 protein 2

Gene names

Name:	SYF2
Synonyms:	NTC31
Ordered Locus Names:	YGR129W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing and cell cycle control. As a component of the NTC complex (or PRP19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. The cell cycle arrest of SYF2 defective cells may be due to the inefficient splicing of TUB1. Ref.4 Ref.5 Ref.8
Subunit structure	Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CEF1, CLF1 and SYF1. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus Ref.9.
Miscellaneous	Present with 1670 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SYF2 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	Prp19 complex Inferred from direct assay Ref.7. Source: SGD U2-type catalytic step 1 spliceosome Inferred from direct assay Ref.5 Ref.7. Source: SGD U2-type catalytic step 2 spliceosome Inferred from direct assay Ref.5. Source: SGD U2-type post-mRNA release spliceosomal complex Inferred from direct assay Ref.5. Source: SGD U2-type prespliceosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CEF1	Q03654	3	EBI-23308,EBI-476
CLF1	Q12309	2	EBI-23308,EBI-484
NTC20	P38302	2	EBI-23308,EBI-20921
PRP19	P32523	4	EBI-23308,EBI-493
SYF1	Q04048	4	EBI-23308,EBI-540

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 215

215

Pre-mRNA-splicing factor SYF2

PRO_0000072381

Amino acid modifications

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphoserine Ref.13

Modified residue

124

Phosphoserine Ref.11 Ref.12 Ref.13

Modified residue

125

Phosphoserine Ref.11 Ref.12 Ref.13

Sequences

Sequence

Length

Mass (Da)

Tools

P53277 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: C80B872FCD808E1D
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FASTA

215

24,803

        10         20         30         40         50         60 
MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN DADESVGDTE 

        70         80         90        100        110        120 
SPEKEKAFHY TVQEYDAWER RHPQGKTGQS QRGGISYDQL AKLSYEKTLR NLATQTQNSS 

       130        140        150        160        170        180 
KQDSSADEED NKNVPKKGRI GKVQKDTKTG KITIADDDKL VNKLAVSLQS ESKKRYEARK 

       190        200        210 
RQMQNAKTLY GVESFINDKN KQFNEKLSRE SKGSE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae." Ben-Yehuda S., Dix I., Russell C.S., McGarvey M., Beggs J.D., Kupiec M. Genetics 156:1503-1517(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CEF1; CLF1 AND SYF1.
[5]	"Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae." Russell C.S., Ben-Yehuda S., Dix I., Kupiec M., Beggs J.D. RNA 6:1565-1572(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SPLICEOSOME.
[6]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[7]	"Functional and physical interactions between components of the Prp19p-associated complex." Chen C.-H., Yu W.-C., Tsao T.Y., Wang L.-Y., Chen H.-R., Lin J.-Y., Tsai W.-Y., Cheng S.-C. Nucleic Acids Res. 30:1029-1037(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
[8]	"Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest through activation of the spindle checkpoint." Dahan O., Kupiec M. Nucleic Acids Res. 30:4361-4370(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, GENETIC INTERACTION WITH ISY1.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-125, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-125, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-61; SER-124 AND SER-125, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72914 Genomic DNA. Translation: CAA97142.1. AY558326 Genomic DNA. Translation: AAS56652.1. BK006941 Genomic DNA. Translation: DAA08222.1.
PIR	S64438.
RefSeq	NP_011645.3. NM_001181258.3.
3D structure databases
ProteinModelPortal	P53277.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1684N.
IntAct	P53277. 13 interactions.
MINT	MINT-402831.
STRING	4932.YGR129W.
Proteomic databases
PaxDb	P53277.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR129W; YGR129W; YGR129W.
GeneID	853030.
KEGG	sce:YGR129W.
Organism-specific databases
CYGD	YGR129w.
SGD	S000003361. SYF2.
Phylogenomic databases
eggNOG	NOG265019.
HOGENOM	HOG000000933.
KO	K12868.
OMA	KPRVYSM.
OrthoDB	EOG4ZGSP5.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30835-MONOMER.
Gene expression databases
Genevestigator	P53277.
GermOnline	YGR129W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013260. mRNA_splic_SYF2. [Graphical view]
Pfam	PF08231. SYF2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972917.

Entry information

Entry name

SYF2_YEAST

Accession

Primary (citable) accession number: P53277
Secondary accession number(s): D6VUR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents