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P53277

- SYF2_YEAST

UniProt

P53277 - SYF2_YEAST

Protein

Pre-mRNA-splicing factor SYF2

Gene

SYF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing and cell cycle control. As a component of the NTC complex (or PRP19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. The cell cycle arrest of SYF2 defective cells may be due to the inefficient splicing of TUB1.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30835-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA-splicing factor SYF2
    Alternative name(s):
    PRP19 complex protein 31
    Synthetic lethal with CDC40 protein 2
    Gene namesi
    Name:SYF2
    Synonyms:NTC31
    Ordered Locus Names:YGR129W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR129w.
    SGDiS000003361. SYF2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleus Source: SGD
    3. Prp19 complex Source: SGD
    4. U2-type catalytic step 1 spliceosome Source: SGD
    5. U2-type catalytic step 2 spliceosome Source: SGD
    6. U2-type post-mRNA release spliceosomal complex Source: SGD
    7. U2-type prespliceosome Source: SGD

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215Pre-mRNA-splicing factor SYF2PRO_0000072381Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451Phosphoserine1 Publication
    Modified residuei124 – 1241Phosphoserine3 Publications
    Modified residuei125 – 1251Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53277.
    PaxDbiP53277.

    Expressioni

    Gene expression databases

    GenevestigatoriP53277.

    Interactioni

    Subunit structurei

    Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CEF1, CLF1 and SYF1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CEF1Q036543EBI-23308,EBI-476
    CLF1Q123092EBI-23308,EBI-484
    NTC20P383022EBI-23308,EBI-20921
    PRP19P325234EBI-23308,EBI-493
    SYF1Q040484EBI-23308,EBI-540

    Protein-protein interaction databases

    BioGridi33377. 54 interactions.
    DIPiDIP-1684N.
    IntActiP53277. 13 interactions.
    MINTiMINT-402831.
    STRINGi4932.YGR129W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53277.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SYF2 family.Curated

    Phylogenomic databases

    eggNOGiNOG265019.
    HOGENOMiHOG000000933.
    KOiK12868.
    OMAiHAISIHR.
    OrthoDBiEOG7SV16F.

    Family and domain databases

    InterProiIPR013260. mRNA_splic_SYF2.
    [Graphical view]
    PfamiPF08231. SYF2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53277-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN    50
    DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQS QRGGISYDQL 100
    AKLSYEKTLR NLATQTQNSS KQDSSADEED NKNVPKKGRI GKVQKDTKTG 150
    KITIADDDKL VNKLAVSLQS ESKKRYEARK RQMQNAKTLY GVESFINDKN 200
    KQFNEKLSRE SKGSE 215
    Length:215
    Mass (Da):24,803
    Last modified:October 1, 1996 - v1
    Checksum:iC80B872FCD808E1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72914 Genomic DNA. Translation: CAA97142.1.
    AY558326 Genomic DNA. Translation: AAS56652.1.
    BK006941 Genomic DNA. Translation: DAA08222.1.
    PIRiS64438.
    RefSeqiNP_011645.3. NM_001181258.3.

    Genome annotation databases

    EnsemblFungiiYGR129W; YGR129W; YGR129W.
    GeneIDi853030.
    KEGGisce:YGR129W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72914 Genomic DNA. Translation: CAA97142.1 .
    AY558326 Genomic DNA. Translation: AAS56652.1 .
    BK006941 Genomic DNA. Translation: DAA08222.1 .
    PIRi S64438.
    RefSeqi NP_011645.3. NM_001181258.3.

    3D structure databases

    ProteinModelPortali P53277.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33377. 54 interactions.
    DIPi DIP-1684N.
    IntActi P53277. 13 interactions.
    MINTi MINT-402831.
    STRINGi 4932.YGR129W.

    Proteomic databases

    MaxQBi P53277.
    PaxDbi P53277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR129W ; YGR129W ; YGR129W .
    GeneIDi 853030.
    KEGGi sce:YGR129W.

    Organism-specific databases

    CYGDi YGR129w.
    SGDi S000003361. SYF2.

    Phylogenomic databases

    eggNOGi NOG265019.
    HOGENOMi HOG000000933.
    KOi K12868.
    OMAi HAISIHR.
    OrthoDBi EOG7SV16F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30835-MONOMER.

    Miscellaneous databases

    NextBioi 972917.

    Gene expression databases

    Genevestigatori P53277.

    Family and domain databases

    InterProi IPR013260. mRNA_splic_SYF2.
    [Graphical view ]
    Pfami PF08231. SYF2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae."
      Ben-Yehuda S., Dix I., Russell C.S., McGarvey M., Beggs J.D., Kupiec M.
      Genetics 156:1503-1517(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CEF1; CLF1 AND SYF1.
    5. "Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae."
      Russell C.S., Ben-Yehuda S., Dix I., Kupiec M., Beggs J.D.
      RNA 6:1565-1572(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE SPLICEOSOME.
    6. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
      Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
      Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Functional and physical interactions between components of the Prp19p-associated complex."
      Chen C.-H., Yu W.-C., Tsao T.Y., Wang L.-Y., Chen H.-R., Lin J.-Y., Tsai W.-Y., Cheng S.-C.
      Nucleic Acids Res. 30:1029-1037(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PRP19-ASSOCIATED COMPLEX.
    8. "Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest through activation of the spindle checkpoint."
      Dahan O., Kupiec M.
      Nucleic Acids Res. 30:4361-4370(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, GENETIC INTERACTION WITH ISY1.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-125, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-124 AND SER-125, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124 AND SER-125, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYF2_YEAST
    AccessioniPrimary (citable) accession number: P53277
    Secondary accession number(s): D6VUR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1670 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3