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Protein

Pre-mRNA-splicing factor SYF2

Gene

SYF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing and cell cycle control. As a component of the NTC complex (or PRP19-associated complex), associates to the spliceosome to mediate conformational rearrangement or to stabilize the structure of the spliceosome after U4 snRNA dissociation, which leads to spliceosome maturation. The cell cycle arrest of SYF2 defective cells may be due to the inefficient splicing of TUB1.3 Publications

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30835-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor SYF2
Alternative name(s):
PRP19 complex protein 31
Synthetic lethal with CDC40 protein 2
Gene namesi
Name:SYF2
Synonyms:NTC31
Ordered Locus Names:YGR129W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR129W
SGDiS000003361 SYF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000723811 – 215Pre-mRNA-splicing factor SYF2Add BLAST215

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45PhosphoserineCombined sources1
Modified residuei124PhosphoserineCombined sources1
Modified residuei125PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53277
PaxDbiP53277
PRIDEiP53277

PTM databases

iPTMnetiP53277

Interactioni

Subunit structurei

Belongs to the NTC complex (or PRP19-associated complex), composed of at least CEF1, CLF1, ISY1, NTC20, SNT309, SYF1, SYF2, and PRP19. The NTC complex associates with the spliceosome after the release of the U1 and U4 snRNAs and forms the CWC spliceosome subcomplex (or CEF1-associated complex) reminiscent of a late-stage spliceosome composed also of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, LEA1, MSL1, PRP8, PRP9, PRP11, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNU114, SPP2, RSE1 and YJU2. Interacts with CEF1, CLF1 and SYF1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33377110 interactors.
DIPiDIP-1684N
IntActiP53277 20 interactors.
MINTiP53277
STRINGi4932.YGR129W

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi97 – 122Combined sources26
Beta strandi147 – 149Combined sources3
Helixi161 – 189Combined sources29
Helixi198 – 210Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50f1-215[»]
5GMKelectron microscopy3.40I1-215[»]
5MPSelectron microscopy3.85y1-215[»]
5MQ0electron microscopy4.17y1-215[»]
5WSGelectron microscopy4.00I1-215[»]
6BK8electron microscopy3.30R1-215[»]
6EXNelectron microscopy3.70y1-215[»]
ProteinModelPortaliP53277
SMRiP53277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SYF2 family.Curated

Phylogenomic databases

HOGENOMiHOG000000933
InParanoidiP53277
KOiK12868
OMAiKPRVYSM
OrthoDBiEOG092C4OA3

Family and domain databases

InterProiView protein in InterPro
IPR013260 mRNA_splic_SYF2
PfamiView protein in Pfam
PF08231 SYF2, 1 hit

Sequencei

Sequence statusi: Complete.

P53277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN
60 70 80 90 100
DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQS QRGGISYDQL
110 120 130 140 150
AKLSYEKTLR NLATQTQNSS KQDSSADEED NKNVPKKGRI GKVQKDTKTG
160 170 180 190 200
KITIADDDKL VNKLAVSLQS ESKKRYEARK RQMQNAKTLY GVESFINDKN
210
KQFNEKLSRE SKGSE
Length:215
Mass (Da):24,803
Last modified:October 1, 1996 - v1
Checksum:iC80B872FCD808E1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72914 Genomic DNA Translation: CAA97142.1
AY558326 Genomic DNA Translation: AAS56652.1
BK006941 Genomic DNA Translation: DAA08222.1
PIRiS64438
RefSeqiNP_011645.3, NM_001181258.3

Genome annotation databases

EnsemblFungiiYGR129W; YGR129W; YGR129W
GeneIDi853030
KEGGisce:YGR129W

Similar proteinsi

Entry informationi

Entry nameiSYF2_YEAST
AccessioniPrimary (citable) accession number: P53277
Secondary accession number(s): D6VUR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome