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P53276

- UTP8_YEAST

UniProt

P53276 - UTP8_YEAST

Protein

U3 small nucleolar RNA-associated protein 8

Gene

UTP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in nucleolar processing of pre-18S ribosomal RNA. Also has a role in nuclear tRNA export. It acts between the steps of tRNA maturation/aminoacylation and its subsequent translocation out of the nucleus. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. snoRNA binding Source: SGD
    3. tRNA binding Source: SGD

    GO - Biological processi

    1. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. positive regulation of transcription from RNA polymerase I promoter Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW
    4. tRNA export from nucleus Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing, Transcription

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30834-MONOMER.

    Protein family/group databases

    TCDBi9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U3 small nucleolar RNA-associated protein 8
    Short name:
    U3 snoRNA-associated protein 8
    Alternative name(s):
    U three protein 8
    U3 protein 8 required for transcription
    t-UTP8
    Gene namesi
    Name:UTP8
    Ordered Locus Names:YGR128C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR128c.
    SGDiS000003360. UTP8.

    Subcellular locationi

    Nucleusnucleolus 3 Publications
    Note: Associated with ribosomal chromatin, even in the absence of transcription.

    GO - Cellular componenti

    1. nucleolus Source: SGD
    2. rDNA heterochromatin Source: SGD
    3. small-subunit processome Source: SGD
    4. t-UTP complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 713713U3 small nucleolar RNA-associated protein 8PRO_0000065743Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei95 – 951Phosphothreonine1 Publication
    Modified residuei148 – 1481Phosphoserine1 Publication
    Modified residuei150 – 1501Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53276.
    PaxDbiP53276.
    PeptideAtlasiP53276.

    Expressioni

    Gene expression databases

    GenevestigatoriP53276.

    Interactioni

    Subunit structurei

    Interacts with snoRNA U3. Interacts with MPP10 and UTP25. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    UTP15Q043054EBI-23301,EBI-28183
    UTP4Q066793EBI-23301,EBI-35712
    UTP9P388823EBI-23301,EBI-24892

    Protein-protein interaction databases

    BioGridi33376. 67 interactions.
    DIPiDIP-6747N.
    IntActiP53276. 25 interactions.
    MINTiMINT-623908.
    STRINGi4932.YGR128C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG39799.
    HOGENOMiHOG000141862.
    KOiK14547.
    OMAiSECLGKS.
    OrthoDBiEOG7SV13X.

    Family and domain databases

    InterProiIPR018843. U3_sm_nucleolar_RNA_assoc_p8.
    [Graphical view]
    PfamiPF10395. Utp8. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53276-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS    50
    SISQYIINPT PKLTFDYPIP STNIITACNA EKGQANIDGN IEASTDDEAN 100
    NEKTINTQKK RNVEIWAFGL MVNKGNYTLN VITKALEDTT DTSNDHLSES 150
    DIDNKAYTGS DEFLSQYKIK AKAKVMSIKI DTKNSLVIAI LQNGLIEIFD 200
    FKLTLLHSFD ISYDNLKYAK WFTENGTEYV FVLCPLQDDK VCYKLLELTD 250
    CGSGESSPIK ELSSTIIEGF SFENSKLCYQ FGKLYKLNQG KIYIYSLPHC 300
    QLQQVIEFPM VDKLSPGDDL ISFQPVSVNR VLLTVNNVIY LLDLLHCSTL 350
    SQRELTHVKT FQLLKSAVIN SEKSHNSKTI AIGISTKNGP NPTSSLEIIN 400
    IDVGTNTLKD SLGKSFQVGN NDSSVILKPL FDDKDINDKR VKCNDVSGDS 450
    SVPVLHCNEV IEKLSALQDN DITSFDDIFF KELKIKEEHY TEKDRYISDP 500
    GFLNKVLDLI FGKFSGNDYP KTLTFLLTHP LFPLSRTRNL LSLLRDQPRL 550
    FKQAIVTCPN LPLNELLEEL FSIRNRELLL DISFRILQDF TRDSIKQEMK 600
    KLSKLDVQNF IEFITSGGED SSPECFNPSQ STQLFQLLSL VLDSIGLFSL 650
    EGALLENLTL YIDKQVEIAE RNTELWNLID TKGFQHGFAS STFDNGTSQK 700
    RALPTYTMEY LDI 713
    Length:713
    Mass (Da):80,191
    Last modified:October 1, 1996 - v1
    Checksum:i6F5AE1492F841F49
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72913 Genomic DNA. Translation: CAA97140.1.
    BK006941 Genomic DNA. Translation: DAA08221.1.
    PIRiS64437.
    RefSeqiNP_011644.3. NM_001181257.3.

    Genome annotation databases

    EnsemblFungiiYGR128C; YGR128C; YGR128C.
    GeneIDi853029.
    KEGGisce:YGR128C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72913 Genomic DNA. Translation: CAA97140.1 .
    BK006941 Genomic DNA. Translation: DAA08221.1 .
    PIRi S64437.
    RefSeqi NP_011644.3. NM_001181257.3.

    3D structure databases

    ProteinModelPortali P53276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33376. 67 interactions.
    DIPi DIP-6747N.
    IntActi P53276. 25 interactions.
    MINTi MINT-623908.
    STRINGi 4932.YGR128C.

    Protein family/group databases

    TCDBi 9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

    Proteomic databases

    MaxQBi P53276.
    PaxDbi P53276.
    PeptideAtlasi P53276.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR128C ; YGR128C ; YGR128C .
    GeneIDi 853029.
    KEGGi sce:YGR128C.

    Organism-specific databases

    CYGDi YGR128c.
    SGDi S000003360. UTP8.

    Phylogenomic databases

    eggNOGi NOG39799.
    HOGENOMi HOG000141862.
    KOi K14547.
    OMAi SECLGKS.
    OrthoDBi EOG7SV13X.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30834-MONOMER.

    Miscellaneous databases

    NextBioi 972914.

    Gene expression databases

    Genevestigatori P53276.

    Family and domain databases

    InterProi IPR018843. U3_sm_nucleolar_RNA_assoc_p8.
    [Graphical view ]
    Pfami PF10395. Utp8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    4. "Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae."
      Steiner-Mosonyi M., Leslie D.M., Dehghani H., Aitchison J.D., Mangroo D.
      J. Biol. Chem. 278:32236-32245(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components."
      Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J.
      Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-95 AND SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The DEAD-box RNA helicase-like Utp25 is an SSU processome component."
      Charette J.M., Baserga S.J.
      RNA 16:2156-2169(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UTP25.

    Entry informationi

    Entry nameiUTP8_YEAST
    AccessioniPrimary (citable) accession number: P53276
    Secondary accession number(s): D6VUR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 16800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3