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Protein

U3 small nucleolar RNA-associated protein 8

Gene

UTP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in nucleolar processing of pre-18S ribosomal RNA. Also has a role in nuclear tRNA export. It acts between the steps of tRNA maturation/aminoacylation and its subsequent translocation out of the nucleus. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).3 Publications

Miscellaneous

Present with 16800 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • snoRNA binding Source: SGD
  • tRNA binding Source: SGD

GO - Biological processi

  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • positive regulation of transcription by RNA polymerase I Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA export from nucleus Source: SGD

Keywordsi

Molecular functionRibonucleoprotein
Biological processRibosome biogenesis, rRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-30834-MONOMER

Protein family/group databases

TCDBi9.A.50.1.1 the nuclear t-rna exporter (t-exporter) family

Names & Taxonomyi

Protein namesi
Recommended name:
U3 small nucleolar RNA-associated protein 8
Short name:
U3 snoRNA-associated protein 8
Alternative name(s):
U three protein 8
U3 protein 8 required for transcription
t-UTP8
Gene namesi
Name:UTP8
Ordered Locus Names:YGR128C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR128C
SGDiS000003360 UTP8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657431 – 713U3 small nucleolar RNA-associated protein 8Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphothreonineCombined sources1
Modified residuei148PhosphoserineCombined sources1
Modified residuei150PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53276
PaxDbiP53276
PRIDEiP53276

PTM databases

iPTMnetiP53276

Interactioni

Subunit structurei

Interacts with snoRNA U3. Interacts with MPP10 and UTP25. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33376351 interactors.
DIPiDIP-6747N
IntActiP53276 45 interactors.
MINTiP53276
STRINGi4932.YGR128C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WLCelectron microscopy3.80LI549-713[»]
ProteinModelPortaliP53276
SMRiP53276
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000141862
InParanoidiP53276
KOiK14547
OMAiTIVECMD
OrthoDBiEOG092C2WJ4

Family and domain databases

InterProiView protein in InterPro
IPR018843 Utp8
PfamiView protein in Pfam
PF10395 Utp8, 1 hit

Sequencei

Sequence statusi: Complete.

P53276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS
60 70 80 90 100
SISQYIINPT PKLTFDYPIP STNIITACNA EKGQANIDGN IEASTDDEAN
110 120 130 140 150
NEKTINTQKK RNVEIWAFGL MVNKGNYTLN VITKALEDTT DTSNDHLSES
160 170 180 190 200
DIDNKAYTGS DEFLSQYKIK AKAKVMSIKI DTKNSLVIAI LQNGLIEIFD
210 220 230 240 250
FKLTLLHSFD ISYDNLKYAK WFTENGTEYV FVLCPLQDDK VCYKLLELTD
260 270 280 290 300
CGSGESSPIK ELSSTIIEGF SFENSKLCYQ FGKLYKLNQG KIYIYSLPHC
310 320 330 340 350
QLQQVIEFPM VDKLSPGDDL ISFQPVSVNR VLLTVNNVIY LLDLLHCSTL
360 370 380 390 400
SQRELTHVKT FQLLKSAVIN SEKSHNSKTI AIGISTKNGP NPTSSLEIIN
410 420 430 440 450
IDVGTNTLKD SLGKSFQVGN NDSSVILKPL FDDKDINDKR VKCNDVSGDS
460 470 480 490 500
SVPVLHCNEV IEKLSALQDN DITSFDDIFF KELKIKEEHY TEKDRYISDP
510 520 530 540 550
GFLNKVLDLI FGKFSGNDYP KTLTFLLTHP LFPLSRTRNL LSLLRDQPRL
560 570 580 590 600
FKQAIVTCPN LPLNELLEEL FSIRNRELLL DISFRILQDF TRDSIKQEMK
610 620 630 640 650
KLSKLDVQNF IEFITSGGED SSPECFNPSQ STQLFQLLSL VLDSIGLFSL
660 670 680 690 700
EGALLENLTL YIDKQVEIAE RNTELWNLID TKGFQHGFAS STFDNGTSQK
710
RALPTYTMEY LDI
Length:713
Mass (Da):80,191
Last modified:October 1, 1996 - v1
Checksum:i6F5AE1492F841F49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72913 Genomic DNA Translation: CAA97140.1
BK006941 Genomic DNA Translation: DAA08221.1
PIRiS64437
RefSeqiNP_011644.3, NM_001181257.3

Genome annotation databases

EnsemblFungiiYGR128C; YGR128C; YGR128C
GeneIDi853029
KEGGisce:YGR128C

Similar proteinsi

Entry informationi

Entry nameiUTP8_YEAST
AccessioniPrimary (citable) accession number: P53276
Secondary accession number(s): D6VUR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome