P53276 (UTP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 8
Short name=U3 snoRNA-associated protein 8

Alternative name(s):
U three protein 8
U3 protein 8 required for transcription
t-UTP8

Gene names

Name:	UTP8
Ordered Locus Names:	YGR128C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Also has a role in nuclear tRNA export. It acts between the steps of tRNA maturation/aminoacylation and its subsequent translocation out of the nucleus. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Ref.3 Ref.4 Ref.6
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10 and UTP25. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs. Ref.3 Ref.6 Ref.9
Subcellular location	Nucleus › nucleolus. Note: Associated with ribosomal chromatin, even in the absence of transcription. Ref.3 Ref.4 Ref.6
Miscellaneous	Present with 16800 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transcription rRNA processing
Cellular component	Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.3. Source: SGD positive regulation of transcription from RNA polymerase I promoter Inferred from mutant phenotype Ref.6. Source: SGD tRNA export from nucleus Inferred from mutant phenotype Ref.4. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	rDNA heterochromatin Inferred from direct assay Ref.6. Source: SGD small-subunit processome Inferred from direct assay Ref.3. Source: SGD t-UTP complex Inferred from direct assay PubMed 17515605. Source: SGD
Molecular_function	snoRNA binding Inferred from physical interaction Ref.3. Source: SGD tRNA binding Inferred from direct assay PubMed 19370060. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UTP15	Q04305	4	EBI-23301,EBI-28183
UTP4	Q06679	3	EBI-23301,EBI-35712
UTP9	P38882	3	EBI-23301,EBI-24892

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	U3 small nucleolar RNA-associated protein 8		PRO_0000065743
Amino acid modifications
	Modified residue	94	1	Phosphoserine Ref.8
	Modified residue	148	1	Phosphoserine Ref.8
	Modified residue	150	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P53276 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6F5AE1492F841F49	FASTA	713	80,191
	10 20 30 40 50 60 MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS SISQYIINPT 70 80 90 100 110 120 PKLTFDYPIP STNIITACNA EKGQANIDGN IEASTDDEAN NEKTINTQKK RNVEIWAFGL 130 140 150 160 170 180 MVNKGNYTLN VITKALEDTT DTSNDHLSES DIDNKAYTGS DEFLSQYKIK AKAKVMSIKI 190 200 210 220 230 240 DTKNSLVIAI LQNGLIEIFD FKLTLLHSFD ISYDNLKYAK WFTENGTEYV FVLCPLQDDK 250 260 270 280 290 300 VCYKLLELTD CGSGESSPIK ELSSTIIEGF SFENSKLCYQ FGKLYKLNQG KIYIYSLPHC 310 320 330 340 350 360 QLQQVIEFPM VDKLSPGDDL ISFQPVSVNR VLLTVNNVIY LLDLLHCSTL SQRELTHVKT 370 380 390 400 410 420 FQLLKSAVIN SEKSHNSKTI AIGISTKNGP NPTSSLEIIN IDVGTNTLKD SLGKSFQVGN 430 440 450 460 470 480 NDSSVILKPL FDDKDINDKR VKCNDVSGDS SVPVLHCNEV IEKLSALQDN DITSFDDIFF 490 500 510 520 530 540 KELKIKEEHY TEKDRYISDP GFLNKVLDLI FGKFSGNDYP KTLTFLLTHP LFPLSRTRNL 550 560 570 580 590 600 LSLLRDQPRL FKQAIVTCPN LPLNELLEEL FSIRNRELLL DISFRILQDF TRDSIKQEMK 610 620 630 640 650 660 KLSKLDVQNF IEFITSGGED SSPECFNPSQ STQLFQLLSL VLDSIGLFSL EGALLENLTL 670 680 690 700 710 YIDKQVEIAE RNTELWNLID TKGFQHGFAS STFDNGTSQK RALPTYTMEY LDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[4]	"Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae." Steiner-Mosonyi M., Leslie D.M., Dehghani H., Aitchison J.D., Mangroo D. J. Biol. Chem. 278:32236-32245(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components." Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J. Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94 AND SER-148, MASS SPECTROMETRY.
[9]	"The DEAD-box RNA helicase-like Utp25 is an SSU processome component." Charette J.M., Baserga S.J. RNA 16:2156-2169(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UTP25.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72913 Genomic DNA. Translation: CAA97140.1. BK006941 Genomic DNA. Translation: DAA08221.1.
PIR	S64437.
RefSeq	NP_011644.3. NM_001181257.3.
3D structure databases
ProteinModelPortal	P53276.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6747N.
IntAct	P53276. 24 interactions.
MINT	MINT-623908.
STRING	4932.YGR128C.
Protein family/group databases
TCDB	9.A.50.1.1. nuclear t-RNA exporter (t-Exporter) family.
Proteomic databases
PaxDb	P53276.
PeptideAtlas	P53276.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR128C; YGR128C; YGR128C.
GeneID	853029.
KEGG	sce:YGR128C. sce:YGR134W.
Organism-specific databases
CYGD	YGR128c.
SGD	S000003360. UTP8.
Phylogenomic databases
eggNOG	NOG39799.
HOGENOM	HOG000141862.
KO	K14547.
OMA	SECLGKS.
OrthoDB	EOG49KK02.
Gene expression databases
Genevestigator	P53276.
GermOnline	YGR128C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018843. U3_sm_nucleolar_RNA_assoc_p8. [Graphical view]
Pfam	PF10395. Utp8. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972914.

Entry information

Entry name

UTP8_YEAST

Accession

Primary (citable) accession number: P53276
Secondary accession number(s): D6VUR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program