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P53276

- UTP8_YEAST

UniProt

P53276 - UTP8_YEAST

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Protein

U3 small nucleolar RNA-associated protein 8

Gene
UTP8, YGR128C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in nucleolar processing of pre-18S ribosomal RNA. Also has a role in nuclear tRNA export. It acts between the steps of tRNA maturation/aminoacylation and its subsequent translocation out of the nucleus. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs).3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. snoRNA binding Source: SGD
  3. tRNA binding Source: SGD

GO - Biological processi

  1. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. positive regulation of transcription from RNA polymerase I promoter Source: SGD
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. tRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-30834-MONOMER.

Protein family/group databases

TCDBi9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

Names & Taxonomyi

Protein namesi
Recommended name:
U3 small nucleolar RNA-associated protein 8
Short name:
U3 snoRNA-associated protein 8
Alternative name(s):
U three protein 8
U3 protein 8 required for transcription
t-UTP8
Gene namesi
Name:UTP8
Ordered Locus Names:YGR128C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR128c.
SGDiS000003360. UTP8.

Subcellular locationi

Nucleusnucleolus
Note: Associated with ribosomal chromatin, even in the absence of transcription.3 Publications

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. rDNA heterochromatin Source: SGD
  3. small-subunit processome Source: SGD
  4. t-UTP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 713713U3 small nucleolar RNA-associated protein 8PRO_0000065743Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951Phosphothreonine1 Publication
Modified residuei148 – 1481Phosphoserine1 Publication
Modified residuei150 – 1501Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53276.
PaxDbiP53276.
PeptideAtlasiP53276.

Expressioni

Gene expression databases

GenevestigatoriP53276.

Interactioni

Subunit structurei

Interacts with snoRNA U3. Interacts with MPP10 and UTP25. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UTP15Q043054EBI-23301,EBI-28183
UTP4Q066793EBI-23301,EBI-35712
UTP9P388823EBI-23301,EBI-24892

Protein-protein interaction databases

BioGridi33376. 67 interactions.
DIPiDIP-6747N.
IntActiP53276. 25 interactions.
MINTiMINT-623908.
STRINGi4932.YGR128C.

Structurei

3D structure databases

ProteinModelPortaliP53276.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG39799.
HOGENOMiHOG000141862.
KOiK14547.
OMAiSECLGKS.
OrthoDBiEOG7SV13X.

Family and domain databases

InterProiIPR018843. U3_sm_nucleolar_RNA_assoc_p8.
[Graphical view]
PfamiPF10395. Utp8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53276-1 [UniParc]FASTAAdd to Basket

« Hide

MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS    50
SISQYIINPT PKLTFDYPIP STNIITACNA EKGQANIDGN IEASTDDEAN 100
NEKTINTQKK RNVEIWAFGL MVNKGNYTLN VITKALEDTT DTSNDHLSES 150
DIDNKAYTGS DEFLSQYKIK AKAKVMSIKI DTKNSLVIAI LQNGLIEIFD 200
FKLTLLHSFD ISYDNLKYAK WFTENGTEYV FVLCPLQDDK VCYKLLELTD 250
CGSGESSPIK ELSSTIIEGF SFENSKLCYQ FGKLYKLNQG KIYIYSLPHC 300
QLQQVIEFPM VDKLSPGDDL ISFQPVSVNR VLLTVNNVIY LLDLLHCSTL 350
SQRELTHVKT FQLLKSAVIN SEKSHNSKTI AIGISTKNGP NPTSSLEIIN 400
IDVGTNTLKD SLGKSFQVGN NDSSVILKPL FDDKDINDKR VKCNDVSGDS 450
SVPVLHCNEV IEKLSALQDN DITSFDDIFF KELKIKEEHY TEKDRYISDP 500
GFLNKVLDLI FGKFSGNDYP KTLTFLLTHP LFPLSRTRNL LSLLRDQPRL 550
FKQAIVTCPN LPLNELLEEL FSIRNRELLL DISFRILQDF TRDSIKQEMK 600
KLSKLDVQNF IEFITSGGED SSPECFNPSQ STQLFQLLSL VLDSIGLFSL 650
EGALLENLTL YIDKQVEIAE RNTELWNLID TKGFQHGFAS STFDNGTSQK 700
RALPTYTMEY LDI 713
Length:713
Mass (Da):80,191
Last modified:October 1, 1996 - v1
Checksum:i6F5AE1492F841F49
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72913 Genomic DNA. Translation: CAA97140.1.
BK006941 Genomic DNA. Translation: DAA08221.1.
PIRiS64437.
RefSeqiNP_011644.3. NM_001181257.3.

Genome annotation databases

EnsemblFungiiYGR128C; YGR128C; YGR128C.
GeneIDi853029.
KEGGisce:YGR128C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72913 Genomic DNA. Translation: CAA97140.1 .
BK006941 Genomic DNA. Translation: DAA08221.1 .
PIRi S64437.
RefSeqi NP_011644.3. NM_001181257.3.

3D structure databases

ProteinModelPortali P53276.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33376. 67 interactions.
DIPi DIP-6747N.
IntActi P53276. 25 interactions.
MINTi MINT-623908.
STRINGi 4932.YGR128C.

Protein family/group databases

TCDBi 9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

Proteomic databases

MaxQBi P53276.
PaxDbi P53276.
PeptideAtlasi P53276.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR128C ; YGR128C ; YGR128C .
GeneIDi 853029.
KEGGi sce:YGR128C.

Organism-specific databases

CYGDi YGR128c.
SGDi S000003360. UTP8.

Phylogenomic databases

eggNOGi NOG39799.
HOGENOMi HOG000141862.
KOi K14547.
OMAi SECLGKS.
OrthoDBi EOG7SV13X.

Enzyme and pathway databases

BioCyci YEAST:G3O-30834-MONOMER.

Miscellaneous databases

NextBioi 972914.

Gene expression databases

Genevestigatori P53276.

Family and domain databases

InterProi IPR018843. U3_sm_nucleolar_RNA_assoc_p8.
[Graphical view ]
Pfami PF10395. Utp8. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  4. "Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae."
    Steiner-Mosonyi M., Leslie D.M., Dehghani H., Aitchison J.D., Mangroo D.
    J. Biol. Chem. 278:32236-32245(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components."
    Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J.
    Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-95 AND SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The DEAD-box RNA helicase-like Utp25 is an SSU processome component."
    Charette J.M., Baserga S.J.
    RNA 16:2156-2169(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UTP25.

Entry informationi

Entry nameiUTP8_YEAST
AccessioniPrimary (citable) accession number: P53276
Secondary accession number(s): D6VUR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16800 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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