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P53276 (UTP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U3 small nucleolar RNA-associated protein 8

Short name=U3 snoRNA-associated protein 8
Alternative name(s):
U three protein 8
U3 protein 8 required for transcription
t-UTP8
Gene names
Name:UTP8
Ordered Locus Names:YGR128C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length713 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in nucleolar processing of pre-18S ribosomal RNA. Also has a role in nuclear tRNA export. It acts between the steps of tRNA maturation/aminoacylation and its subsequent translocation out of the nucleus. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Ref.3 Ref.4 Ref.6

Subunit structure

Interacts with snoRNA U3. Interacts with MPP10 and UTP25. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs. Ref.3 Ref.6 Ref.10

Subcellular location

Nucleusnucleolus. Note: Associated with ribosomal chromatin, even in the absence of transcription. Ref.3 Ref.4 Ref.6

Miscellaneous

Present with 16800 molecules/cell in log phase SD medium.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 713713U3 small nucleolar RNA-associated protein 8
PRO_0000065743

Amino acid modifications

Modified residue951Phosphothreonine Ref.9
Modified residue1481Phosphoserine Ref.8
Modified residue1501Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P53276 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 6F5AE1492F841F49

FASTA71380,191
        10         20         30         40         50         60 
MPSLSQPFRL ATLPKIASLS NFSLQADYVQ VADGTFNEST NNITLGISGS SISQYIINPT 

        70         80         90        100        110        120 
PKLTFDYPIP STNIITACNA EKGQANIDGN IEASTDDEAN NEKTINTQKK RNVEIWAFGL 

       130        140        150        160        170        180 
MVNKGNYTLN VITKALEDTT DTSNDHLSES DIDNKAYTGS DEFLSQYKIK AKAKVMSIKI 

       190        200        210        220        230        240 
DTKNSLVIAI LQNGLIEIFD FKLTLLHSFD ISYDNLKYAK WFTENGTEYV FVLCPLQDDK 

       250        260        270        280        290        300 
VCYKLLELTD CGSGESSPIK ELSSTIIEGF SFENSKLCYQ FGKLYKLNQG KIYIYSLPHC 

       310        320        330        340        350        360 
QLQQVIEFPM VDKLSPGDDL ISFQPVSVNR VLLTVNNVIY LLDLLHCSTL SQRELTHVKT 

       370        380        390        400        410        420 
FQLLKSAVIN SEKSHNSKTI AIGISTKNGP NPTSSLEIIN IDVGTNTLKD SLGKSFQVGN 

       430        440        450        460        470        480 
NDSSVILKPL FDDKDINDKR VKCNDVSGDS SVPVLHCNEV IEKLSALQDN DITSFDDIFF 

       490        500        510        520        530        540 
KELKIKEEHY TEKDRYISDP GFLNKVLDLI FGKFSGNDYP KTLTFLLTHP LFPLSRTRNL 

       550        560        570        580        590        600 
LSLLRDQPRL FKQAIVTCPN LPLNELLEEL FSIRNRELLL DISFRILQDF TRDSIKQEMK 

       610        620        630        640        650        660 
KLSKLDVQNF IEFITSGGED SSPECFNPSQ STQLFQLLSL VLDSIGLFSL EGALLENLTL 

       670        680        690        700        710 
YIDKQVEIAE RNTELWNLID TKGFQHGFAS STFDNGTSQK RALPTYTMEY LDI 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis."
Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.
Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[4]"Utp8p is an essential intranuclear component of the nuclear tRNA export machinery of Saccharomyces cerevisiae."
Steiner-Mosonyi M., Leslie D.M., Dehghani H., Aitchison J.D., Mangroo D.
J. Biol. Chem. 278:32236-32245(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components."
Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J.
Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-95 AND SER-150, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The DEAD-box RNA helicase-like Utp25 is an SSU processome component."
Charette J.M., Baserga S.J.
RNA 16:2156-2169(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UTP25.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72913 Genomic DNA. Translation: CAA97140.1.
BK006941 Genomic DNA. Translation: DAA08221.1.
PIRS64437.
RefSeqNP_011644.3. NM_001181257.3.

3D structure databases

ProteinModelPortalP53276.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33376. 67 interactions.
DIPDIP-6747N.
IntActP53276. 25 interactions.
MINTMINT-623908.
STRING4932.YGR128C.

Protein family/group databases

TCDB9.A.50.1.1. the nuclear t-rna exporter (t-exporter) family.

Proteomic databases

PaxDbP53276.
PeptideAtlasP53276.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR128C; YGR128C; YGR128C.
GeneID853029.
KEGGsce:YGR128C.

Organism-specific databases

CYGDYGR128c.
SGDS000003360. UTP8.

Phylogenomic databases

eggNOGNOG39799.
HOGENOMHOG000141862.
KOK14547.
OMASECLGKS.
OrthoDBEOG7SV13X.

Enzyme and pathway databases

BioCycYEAST:G3O-30834-MONOMER.

Gene expression databases

GenevestigatorP53276.

Family and domain databases

InterProIPR018843. U3_sm_nucleolar_RNA_assoc_p8.
[Graphical view]
PfamPF10395. Utp8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972914.

Entry information

Entry nameUTP8_YEAST
AccessionPrimary (citable) accession number: P53276
Secondary accession number(s): D6VUR0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD