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P53273

- YG35_YEAST

UniProt

P53273 - YG35_YEAST

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Protein

Uncharacterized vacuolar membrane protein YGR125W

Gene

YGR125W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. sulfate transmembrane transporter activity Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30831-MONOMER.

Protein family/group databases

TCDBi2.A.53.3.10. the sulfate permease (sulp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized vacuolar membrane protein YGR125W
Gene namesi
Ordered Locus Names:YGR125W
ORF Names:G6362
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR125w.
SGDiS000003357. YGR125W.

Subcellular locationi

Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 213213VacuolarSequence AnalysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence AnalysisAdd
BLAST
Topological domaini235 – 2362CytoplasmicSequence Analysis
Transmembranei237 – 25721HelicalSequence AnalysisAdd
BLAST
Topological domaini258 – 26912VacuolarSequence AnalysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence AnalysisAdd
BLAST
Topological domaini291 – 30010CytoplasmicSequence Analysis
Transmembranei301 – 32121HelicalSequence AnalysisAdd
BLAST
Topological domaini322 – 33817VacuolarSequence AnalysisAdd
BLAST
Transmembranei339 – 35921HelicalSequence AnalysisAdd
BLAST
Topological domaini360 – 37516CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence AnalysisAdd
BLAST
Topological domaini397 – 4059VacuolarSequence Analysis
Transmembranei406 – 42621HelicalSequence AnalysisAdd
BLAST
Topological domaini427 – 47347CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei474 – 49421HelicalSequence AnalysisAdd
BLAST
Topological domaini495 – 51521VacuolarSequence AnalysisAdd
BLAST
Transmembranei516 – 53621HelicalSequence AnalysisAdd
BLAST
Topological domaini537 – 54610CytoplasmicSequence Analysis
Transmembranei547 – 56721HelicalSequence AnalysisAdd
BLAST
Topological domaini568 – 5681VacuolarSequence Analysis
Transmembranei569 – 58921HelicalSequence AnalysisAdd
BLAST
Topological domaini590 – 60415CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei605 – 62521HelicalSequence AnalysisAdd
BLAST
Topological domaini626 – 66439VacuolarSequence AnalysisAdd
BLAST
Transmembranei665 – 68521HelicalSequence AnalysisAdd
BLAST
Topological domaini686 – 1036351CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Uncharacterized vacuolar membrane protein YGR125WPRO_0000202819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421Phosphoserine3 Publications
Modified residuei127 – 1271Phosphoserine1 Publication
Modified residuei130 – 1301Phosphothreonine1 Publication
Modified residuei140 – 1401Phosphoserine2 Publications
Modified residuei144 – 1441Phosphoserine1 Publication
Modified residuei149 – 1491Phosphoserine2 Publications
Modified residuei152 – 1521Phosphoserine1 Publication
Modified residuei153 – 1531Phosphoserine1 Publication
Modified residuei842 – 8421Phosphoserine3 Publications
Modified residuei847 – 8471Phosphothreonine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53273.
PaxDbiP53273.

Expressioni

Gene expression databases

GenevestigatoriP53273.

Interactioni

Protein-protein interaction databases

BioGridi33373. 102 interactions.
DIPiDIP-7435N.
IntActiP53273. 3 interactions.
MINTiMINT-1360603.
STRINGi4932.YGR125W.

Structurei

3D structure databases

ProteinModelPortaliP53273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini660 – 781122STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0659.
HOGENOMiHOG000192103.
InParanoidiP53273.
KOiK03321.
OMAiDYSAAEG.
OrthoDBiEOG75J0WF.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
IPR011547. Sulph_transpt.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
PROSITEiPS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53273-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM
60 70 80 90 100
SGFFTGGNSP MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS
110 120 130 140 150
SSVEENSPTI KDYMDIIGNG DRKDDQSMRT IEENIDEEYS DEYSRLLLSP
160 170 180 190 200
ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ SLRPSHNLRF RPRNLWHMCT
210 220 230 240 250
SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS HLGPTGISMF
260 270 280 290 300
YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE
310 320 330 340 350
IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF
360 370 380 390 400
LIITGIEVTT RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR
410 420 430 440 450
YFKNSLVLPS FYILTLVLFH FIVAIIPTLS LDALRQAGWI FPIANSDSKW
460 470 480 490 500
YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF GILHVPINVP ALAMSLQMDK
510 520 530 540 550
YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA DSPFAGFLLI
560 570 580 590 600
ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY
610 620 630 640 650
LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR
660 670 680 690 700
STVYRDYVQT KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK
710 720 730 740 750
DATKRRIKYL ILDFKNINAD NIDYSAAEGF NRIKRFTETK RIKLIISSIK
760 770 780 790 800
ERDRIYNAFN NVGLLNDVEL FADLNSALEW CENEFLFQYK QLRKKAKERL
810 820 830 840 850
EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL MSLPTNTPRN
860 870 880 890 900
YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV
910 920 930 940 950
QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL
960 970 980 990 1000
PQGTLYEIFS NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN
1010 1020 1030
KLKEDNLALY VEVALMVMCI KDTRFKELLG YTLVSA
Length:1,036
Mass (Da):116,971
Last modified:October 1, 1996 - v1
Checksum:i91A50225CA7FFCB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83099 Genomic DNA. Translation: CAA58161.1.
Z72910 Genomic DNA. Translation: CAA97136.1.
BK006941 Genomic DNA. Translation: DAA08218.1.
PIRiS55984.
RefSeqiNP_011641.1. NM_001181254.1.

Genome annotation databases

EnsemblFungiiYGR125W; YGR125W; YGR125W.
GeneIDi853026.
KEGGisce:YGR125W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83099 Genomic DNA. Translation: CAA58161.1 .
Z72910 Genomic DNA. Translation: CAA97136.1 .
BK006941 Genomic DNA. Translation: DAA08218.1 .
PIRi S55984.
RefSeqi NP_011641.1. NM_001181254.1.

3D structure databases

ProteinModelPortali P53273.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33373. 102 interactions.
DIPi DIP-7435N.
IntActi P53273. 3 interactions.
MINTi MINT-1360603.
STRINGi 4932.YGR125W.

Protein family/group databases

TCDBi 2.A.53.3.10. the sulfate permease (sulp) family.

Proteomic databases

MaxQBi P53273.
PaxDbi P53273.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR125W ; YGR125W ; YGR125W .
GeneIDi 853026.
KEGGi sce:YGR125W.

Organism-specific databases

CYGDi YGR125w.
SGDi S000003357. YGR125W.

Phylogenomic databases

eggNOGi COG0659.
HOGENOMi HOG000192103.
InParanoidi P53273.
KOi K03321.
OMAi DYSAAEG.
OrthoDBi EOG75J0WF.

Enzyme and pathway databases

BioCyci YEAST:G3O-30831-MONOMER.

Miscellaneous databases

NextBioi 972905.

Gene expression databases

Genevestigatori P53273.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
IPR011547. Sulph_transpt.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
PROSITEi PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes."
    van Dyck L., Tettelin H., Purnelle B., Goffeau A.
    Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-149; SER-152; SER-153; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-127; THR-130; SER-140; SER-144; SER-149; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYG35_YEAST
AccessioniPrimary (citable) accession number: P53273
Secondary accession number(s): D6VUQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3