Uncharacterized vacuolar membrane protein YGR125W - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P53273 (YG35_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Uncharacterized vacuolar membrane protein YGR125W

Gene names

Ordered Locus Names:	YGR125W
ORF Names:	G6362

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1036 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 1420 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 STAS domain.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sulfate transport Inferred from electronic annotation. Source: InterPro
Cellular_component	fungal-type vacuole Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sulfate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1036

1036

Uncharacterized vacuolar membrane protein YGR125W

PRO_0000202819

Regions

Topological domain

1 – 213

213

Vacuolar Potential

Transmembrane

214 – 234

Helical; Potential

Topological domain

235 – 236

Cytoplasmic Potential

Transmembrane

237 – 257

Helical; Potential

Topological domain

258 – 269

Vacuolar Potential

Transmembrane

270 – 290

Helical; Potential

Topological domain

291 – 300

Cytoplasmic Potential

Transmembrane

301 – 321

Helical; Potential

Topological domain

322 – 338

Vacuolar Potential

Transmembrane

339 – 359

Helical; Potential

Topological domain

360 – 375

Cytoplasmic Potential

Transmembrane

376 – 396

Helical; Potential

Topological domain

397 – 405

Vacuolar Potential

Transmembrane

406 – 426

Helical; Potential

Topological domain

427 – 473

Cytoplasmic Potential

Transmembrane

474 – 494

Helical; Potential

Topological domain

495 – 515

Vacuolar Potential

Transmembrane

516 – 536

Helical; Potential

Topological domain

537 – 546

Cytoplasmic Potential

Transmembrane

547 – 567

Helical; Potential

Topological domain

568

Vacuolar Potential

Transmembrane

569 – 589

Helical; Potential

Topological domain

590 – 604

Cytoplasmic Potential

Transmembrane

605 – 625

Helical; Potential

Topological domain

626 – 664

Vacuolar Potential

Transmembrane

665 – 685

Helical; Potential

Topological domain

686 – 1036

351

Cytoplasmic Potential

Domain

660 – 781

122

STAS

Amino acid modifications

Modified residue

Phosphoserine Ref.6 Ref.10

Modified residue

101

Phosphoserine Ref.10

Modified residue

139

Phosphotyrosine Ref.10

Modified residue

140

Phosphoserine Ref.6 Ref.8 Ref.10

Modified residue

144

Phosphoserine Ref.10

Modified residue

149

Phosphoserine Ref.8 Ref.10

Modified residue

152

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

153

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

842

Phosphoserine Ref.8 Ref.10

Modified residue

847

Phosphothreonine Ref.8 Ref.10

Modified residue

892

Phosphotyrosine Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P53273 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 91A50225CA7FFCB1
Show »

FASTA

1,036

116,971

        10         20         30         40         50         60 
MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM SGFFTGGNSP 

        70         80         90        100        110        120 
MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS SSVEENSPTI KDYMDIIGNG 

       130        140        150        160        170        180 
DRKDDQSMRT IEENIDEEYS DEYSRLLLSP ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ 

       190        200        210        220        230        240 
SLRPSHNLRF RPRNLWHMCT SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS 

       250        260        270        280        290        300 
HLGPTGISMF YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE 

       310        320        330        340        350        360 
IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF LIITGIEVTT 

       370        380        390        400        410        420 
RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR YFKNSLVLPS FYILTLVLFH 

       430        440        450        460        470        480 
FIVAIIPTLS LDALRQAGWI FPIANSDSKW YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF 

       490        500        510        520        530        540 
GILHVPINVP ALAMSLQMDK YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA 

       550        560        570        580        590        600 
DSPFAGFLLI ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY 

       610        620        630        640        650        660 
LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR STVYRDYVQT 

       670        680        690        700        710        720 
KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK DATKRRIKYL ILDFKNINAD 

       730        740        750        760        770        780 
NIDYSAAEGF NRIKRFTETK RIKLIISSIK ERDRIYNAFN NVGLLNDVEL FADLNSALEW 

       790        800        810        820        830        840 
CENEFLFQYK QLRKKAKERL EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL 

       850        860        870        880        890        900 
MSLPTNTPRN YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV 

       910        920        930        940        950        960 
QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL PQGTLYEIFS 

       970        980        990       1000       1010       1020 
NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN KLKEDNLALY VEVALMVMCI 

      1030 
KDTRFKELLG YTLVSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes." van Dyck L., Tettelin H., Purnelle B., Goffeau A. Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND SER-140, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-149; SER-152; SER-153; SER-842 AND THR-847, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152 AND SER-153, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-101; TYR-139; SER-140; SER-144; SER-149; SER-152; SER-153; SER-842; THR-847 AND TYR-892, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83099 Genomic DNA. Translation: CAA58161.1. Z72910 Genomic DNA. Translation: CAA97136.1. BK006941 Genomic DNA. Translation: DAA08218.1.
PIR	S55984.
RefSeq	NP_011641.1. NM_001181254.1.
3D structure databases
ProteinModelPortal	P53273.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7435N.
IntAct	P53273. 2 interactions.
MINT	MINT-1360603.
STRING	4932.YGR125W.
Proteomic databases
PaxDb	P53273.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR125W; YGR125W; YGR125W.
GeneID	853026.
KEGG	sce:YGR125W.
Organism-specific databases
CYGD	YGR125w.
SGD	S000003357. YGR125W.
Phylogenomic databases
eggNOG	COG0659.
GeneTree	ENSGT00670000099312.
HOGENOM	HOG000192103.
KO	K03321.
OMA	DYSAAEG.
OrthoDB	EOG4QNR4Q.
Gene expression databases
Genevestigator	P53273.
GermOnline	YGR125W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.60.120.10. 1 hit. 3.30.750.24. 1 hit.
InterPro	IPR018490. cNMP-bd-like. IPR000595. cNMP-bd_dom. IPR014710. RmlC-like_jellyroll. IPR002645. STAS_dom. IPR011547. Sulph_transpt. [Graphical view]
Pfam	PF00027. cNMP_binding. 1 hit. PF01740. STAS. 1 hit. PF00916. Sulfate_transp. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 1 hit. SSF52091. STAS. 1 hit.
PROSITE	PS50801. STAS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972905.

Entry information

Entry name

YG35_YEAST

Accession

Primary (citable) accession number: P53273
Secondary accession number(s): D6VUQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents