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P53273

- YG35_YEAST

UniProt

P53273 - YG35_YEAST

Protein

Uncharacterized vacuolar membrane protein YGR125W

Gene

YGR125W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. sulfate transmembrane transporter activity Source: InterPro

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30831-MONOMER.

    Protein family/group databases

    TCDBi2.A.53.3.10. the sulfate permease (sulp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uncharacterized vacuolar membrane protein YGR125W
    Gene namesi
    Ordered Locus Names:YGR125W
    ORF Names:G6362
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR125w.
    SGDiS000003357. YGR125W.

    Subcellular locationi

    Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Uncharacterized vacuolar membrane protein YGR125WPRO_0000202819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421Phosphoserine3 Publications
    Modified residuei127 – 1271Phosphoserine1 Publication
    Modified residuei130 – 1301Phosphothreonine1 Publication
    Modified residuei140 – 1401Phosphoserine2 Publications
    Modified residuei144 – 1441Phosphoserine1 Publication
    Modified residuei149 – 1491Phosphoserine2 Publications
    Modified residuei152 – 1521Phosphoserine1 Publication
    Modified residuei153 – 1531Phosphoserine1 Publication
    Modified residuei842 – 8421Phosphoserine3 Publications
    Modified residuei847 – 8471Phosphothreonine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53273.
    PaxDbiP53273.

    Expressioni

    Gene expression databases

    GenevestigatoriP53273.

    Interactioni

    Protein-protein interaction databases

    BioGridi33373. 102 interactions.
    DIPiDIP-7435N.
    IntActiP53273. 3 interactions.
    MINTiMINT-1360603.
    STRINGi4932.YGR125W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53273.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 213213VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini235 – 2362CytoplasmicSequence Analysis
    Topological domaini258 – 26912VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini291 – 30010CytoplasmicSequence Analysis
    Topological domaini322 – 33817VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini360 – 37516CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini397 – 4059VacuolarSequence Analysis
    Topological domaini427 – 47347CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini495 – 51521VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini537 – 54610CytoplasmicSequence Analysis
    Topological domaini568 – 5681VacuolarSequence Analysis
    Topological domaini590 – 60415CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini626 – 66439VacuolarSequence AnalysisAdd
    BLAST
    Topological domaini686 – 1036351CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei214 – 23421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei270 – 29021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei301 – 32121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei339 – 35921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei376 – 39621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei406 – 42621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei474 – 49421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei516 – 53621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei547 – 56721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei569 – 58921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei605 – 62521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei665 – 68521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini660 – 781122STASPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 STAS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0659.
    HOGENOMiHOG000192103.
    KOiK03321.
    OMAiDYSAAEG.
    OrthoDBiEOG75J0WF.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    3.30.750.24. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR014710. RmlC-like_jellyroll.
    IPR002645. STAS_dom.
    IPR011547. Sulph_transpt.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    SSF52091. SSF52091. 1 hit.
    PROSITEiPS50801. STAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53273-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM     50
    SGFFTGGNSP MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS 100
    SSVEENSPTI KDYMDIIGNG DRKDDQSMRT IEENIDEEYS DEYSRLLLSP 150
    ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ SLRPSHNLRF RPRNLWHMCT 200
    SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS HLGPTGISMF 250
    YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE 300
    IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF 350
    LIITGIEVTT RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR 400
    YFKNSLVLPS FYILTLVLFH FIVAIIPTLS LDALRQAGWI FPIANSDSKW 450
    YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF GILHVPINVP ALAMSLQMDK 500
    YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA DSPFAGFLLI 550
    ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY 600
    LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR 650
    STVYRDYVQT KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK 700
    DATKRRIKYL ILDFKNINAD NIDYSAAEGF NRIKRFTETK RIKLIISSIK 750
    ERDRIYNAFN NVGLLNDVEL FADLNSALEW CENEFLFQYK QLRKKAKERL 800
    EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL MSLPTNTPRN 850
    YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV 900
    QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL 950
    PQGTLYEIFS NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN 1000
    KLKEDNLALY VEVALMVMCI KDTRFKELLG YTLVSA 1036
    Length:1,036
    Mass (Da):116,971
    Last modified:October 1, 1996 - v1
    Checksum:i91A50225CA7FFCB1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83099 Genomic DNA. Translation: CAA58161.1.
    Z72910 Genomic DNA. Translation: CAA97136.1.
    BK006941 Genomic DNA. Translation: DAA08218.1.
    PIRiS55984.
    RefSeqiNP_011641.1. NM_001181254.1.

    Genome annotation databases

    EnsemblFungiiYGR125W; YGR125W; YGR125W.
    GeneIDi853026.
    KEGGisce:YGR125W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83099 Genomic DNA. Translation: CAA58161.1 .
    Z72910 Genomic DNA. Translation: CAA97136.1 .
    BK006941 Genomic DNA. Translation: DAA08218.1 .
    PIRi S55984.
    RefSeqi NP_011641.1. NM_001181254.1.

    3D structure databases

    ProteinModelPortali P53273.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33373. 102 interactions.
    DIPi DIP-7435N.
    IntActi P53273. 3 interactions.
    MINTi MINT-1360603.
    STRINGi 4932.YGR125W.

    Protein family/group databases

    TCDBi 2.A.53.3.10. the sulfate permease (sulp) family.

    Proteomic databases

    MaxQBi P53273.
    PaxDbi P53273.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR125W ; YGR125W ; YGR125W .
    GeneIDi 853026.
    KEGGi sce:YGR125W.

    Organism-specific databases

    CYGDi YGR125w.
    SGDi S000003357. YGR125W.

    Phylogenomic databases

    eggNOGi COG0659.
    HOGENOMi HOG000192103.
    KOi K03321.
    OMAi DYSAAEG.
    OrthoDBi EOG75J0WF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30831-MONOMER.

    Miscellaneous databases

    NextBioi 972905.

    Gene expression databases

    Genevestigatori P53273.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    3.30.750.24. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR014710. RmlC-like_jellyroll.
    IPR002645. STAS_dom.
    IPR011547. Sulph_transpt.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF01740. STAS. 1 hit.
    PF00916. Sulfate_transp. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    SSF52091. SSF52091. 1 hit.
    PROSITEi PS50801. STAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes."
      van Dyck L., Tettelin H., Purnelle B., Goffeau A.
      Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-149; SER-152; SER-153; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-127; THR-130; SER-140; SER-144; SER-149; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYG35_YEAST
    AccessioniPrimary (citable) accession number: P53273
    Secondary accession number(s): D6VUQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1420 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3