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P53273 (YG35_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized vacuolar membrane protein YGR125W
Gene names
Ordered Locus Names:YGR125W
ORF Names:G6362
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1036 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Vacuole membrane; Multi-pass membrane protein Ref.4.

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 STAS domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10361036Uncharacterized vacuolar membrane protein YGR125W
PRO_0000202819

Regions

Topological domain1 – 213213Vacuolar Potential
Transmembrane214 – 23421Helical; Potential
Topological domain235 – 2362Cytoplasmic Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 26912Vacuolar Potential
Transmembrane270 – 29021Helical; Potential
Topological domain291 – 30010Cytoplasmic Potential
Transmembrane301 – 32121Helical; Potential
Topological domain322 – 33817Vacuolar Potential
Transmembrane339 – 35921Helical; Potential
Topological domain360 – 37516Cytoplasmic Potential
Transmembrane376 – 39621Helical; Potential
Topological domain397 – 4059Vacuolar Potential
Transmembrane406 – 42621Helical; Potential
Topological domain427 – 47347Cytoplasmic Potential
Transmembrane474 – 49421Helical; Potential
Topological domain495 – 51521Vacuolar Potential
Transmembrane516 – 53621Helical; Potential
Topological domain537 – 54610Cytoplasmic Potential
Transmembrane547 – 56721Helical; Potential
Topological domain5681Vacuolar Potential
Transmembrane569 – 58921Helical; Potential
Topological domain590 – 60415Cytoplasmic Potential
Transmembrane605 – 62521Helical; Potential
Topological domain626 – 66439Vacuolar Potential
Transmembrane665 – 68521Helical; Potential
Topological domain686 – 1036351Cytoplasmic Potential
Domain660 – 781122STAS

Amino acid modifications

Modified residue421Phosphoserine Ref.6 Ref.9 Ref.10
Modified residue1271Phosphoserine Ref.10
Modified residue1301Phosphothreonine Ref.10
Modified residue1401Phosphoserine Ref.8 Ref.10
Modified residue1441Phosphoserine Ref.10
Modified residue1491Phosphoserine Ref.8 Ref.10
Modified residue1521Phosphoserine Ref.8
Modified residue1531Phosphoserine Ref.8
Modified residue8421Phosphoserine Ref.8 Ref.9 Ref.10
Modified residue8471Phosphothreonine Ref.8 Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P53273 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 91A50225CA7FFCB1

FASTA1,036116,971
        10         20         30         40         50         60 
MGRTIRRRRS NSSLSEAISV SLGINQDSSV NKMHRASVSA MSPPLCRSYM SGFFTGGNSP 

        70         80         90        100        110        120 
MINNLSDSKL PISNKQHPKV IHGSENLHRQ TAQLSNEFCS SSVEENSPTI KDYMDIIGNG 

       130        140        150        160        170        180 
DRKDDQSMRT IEENIDEEYS DEYSRLLLSP ASSNVDDDRN RGLQNSSLPE LEDGYAGGYQ 

       190        200        210        220        230        240 
SLRPSHNLRF RPRNLWHMCT SFPSKFAHYL PAAVLGLLLN ILDALSYGMI IFPITEPVFS 

       250        260        270        280        290        300 
HLGPTGISMF YISTIISQAV YSGGWSSFPS GIGSEMIEIT PFYHTMALAI KEALAGNDDE 

       310        320        330        340        350        360 
IITTTIFCYV ISSMLTGVVF YALGKLRLGK IVGFFPRHIL IGCIGGVGYF LIITGIEVTT 

       370        380        390        400        410        420 
RVAKFEYSWP FFSGLFTDYD TLAKWLLPVL LTVVLIGTQR YFKNSLVLPS FYILTLVLFH 

       430        440        450        460        470        480 
FIVAIIPTLS LDALRQAGWI FPIANSDSKW YDHYRLFNVH KVHWSLVLQQ IPTMMALTFF 

       490        500        510        520        530        540 
GILHVPINVP ALAMSLQMDK YDVDRELIAH GYSNFFSGLL GSVQNYLVYT NSVLFIRAGA 

       550        560        570        580        590        600 
DSPFAGFLLI ALTICIMIIG PVIISFIPIC IVGSLIFLLG YELLVEALVD TWNKLNRFEY 

       610        620        630        640        650        660 
LTVVIIVFTM GIFDFVLGII VGILIACFSF LVDSTKLQTI NGEYNGNVAR STVYRDYVQT 

       670        680        690        700        710        720 
KFLDGIGEQI YVLKLQNLLF FGTIISIEEK IERLLQISNK DATKRRIKYL ILDFKNINAD 

       730        740        750        760        770        780 
NIDYSAAEGF NRIKRFTETK RIKLIISSIK ERDRIYNAFN NVGLLNDVEL FADLNSALEW 

       790        800        810        820        830        840 
CENEFLFQYK QLRKKAKERL EEGKQNNVVS AVIAATKNKK IDTIGNGLNR GSNGDTARNL 

       850        860        870        880        890        900 
MSLPTNTPRN YQILSVAQNV FVNDEQAVKN FKKEYKDDEP VLPILLFALK QYRPDIISEV 

       910        920        930        940        950        960 
QKVREKEIKF WAQLCPYFTR RRLASQSHLL HADNIFFLVE TGMLKATYEL PQGTLYEIFS 

       970        980        990       1000       1010       1020 
NGTCFGKIIA PGNAMPREQK LTIETETDSV LWVIDSSSLN KLKEDNLALY VEVALMVMCI 

      1030 
KDTRFKELLG YTLVSA 

« Hide

References

« Hide 'large scale' references
[1]"An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes."
van Dyck L., Tettelin H., Purnelle B., Goffeau A.
Yeast 13:171-176(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-149; SER-152; SER-153; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42; SER-127; THR-130; SER-140; SER-144; SER-149; SER-842 AND THR-847, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83099 Genomic DNA. Translation: CAA58161.1.
Z72910 Genomic DNA. Translation: CAA97136.1.
BK006941 Genomic DNA. Translation: DAA08218.1.
PIRS55984.
RefSeqNP_011641.1. NM_001181254.1.

3D structure databases

ProteinModelPortalP53273.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33373. 102 interactions.
DIPDIP-7435N.
IntActP53273. 3 interactions.
MINTMINT-1360603.
STRING4932.YGR125W.

Protein family/group databases

TCDB2.A.53.3.10. the sulfate permease (sulp) family.

Proteomic databases

MaxQBP53273.
PaxDbP53273.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR125W; YGR125W; YGR125W.
GeneID853026.
KEGGsce:YGR125W.

Organism-specific databases

CYGDYGR125w.
SGDS000003357. YGR125W.

Phylogenomic databases

eggNOGCOG0659.
HOGENOMHOG000192103.
KOK03321.
OMADYSAAEG.
OrthoDBEOG75J0WF.

Enzyme and pathway databases

BioCycYEAST:G3O-30831-MONOMER.

Gene expression databases

GenevestigatorP53273.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
IPR011547. Sulph_transpt.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
PROSITEPS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972905.

Entry information

Entry nameYG35_YEAST
AccessionPrimary (citable) accession number: P53273
Secondary accession number(s): D6VUQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families