P53262 (VOA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V0 assembly protein 1

Gene names

Name:	VOA1
Ordered Locus Names:	YGR106C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions with VMA21 in assembly of the integral membrane sector (also called V0 complex) of the V-ATPase in the endoplasmic reticulum. Ref.6
Subunit structure	Interacts with VMA21. Associates with the assembling V0 complex. Ref.6
Subcellular location	Vacuole membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.4 Ref.6.
Miscellaneous	Present with 12764 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the VOA1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Vacuole
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein complex assembly Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	endoplasmic reticulum membrane Inferred from mutant phenotype. Source: SGD fungal-type vacuole membrane Inferred from direct assay Ref.4. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 265	241	V0 assembly protein 1		PRO_0000042985
Regions
	Topological domain	25 – 223	199	Vacuolar Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Topological domain	245 – 265	21	Cytoplasmic Potential
	Motif	262 – 265	4	ER retention motif
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53262 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 225292CA9DB35F3D	FASTA	265	29,678
	10 20 30 40 50 60 MVFGQLYALF IFTLSCCISK TVQADSSKES SSFISFDKES NWDTISTISS TADVISSVDS 70 80 90 100 110 120 AIAVFEFDNF SLLDNLMIDE EYPFFNRFFA NDVSLTVHDD SPLNISQSLS PIMEQFTVDE 130 140 150 160 170 180 LPESASDLLY EYSLDDKSIV LFKFTSDAYD LKKLDEFIDS CLSFLEDKSG DNLTVVINSL 190 200 210 220 230 240 GWAFEDEDGD DEYATEETLS HHDNNKGKEG DDDILSSIWT EGLLMCLIVS ALLLFILIVA 250 260 LSWISNLDIT YGALEKSTNP IKKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	Bienvenut W.V., Peters C. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 29-38 AND 144-166, MASS SPECTROMETRY.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Voa1p functions in V-ATPase assembly in the yeast endoplasmic reticulum." Ryan M., Graham L.A., Stevens T.H. Mol. Biol. Cell 19:5131-5142(2008) [PubMed: 18799613] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH VMA21, ASSOCIATION WITH THE V0 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72891 Genomic DNA. Translation: CAA97110.1. BK006941 Genomic DNA. Translation: DAA08199.1.
PIR	S64413.
RefSeq	NP_011620.1. NM_001181235.1.
3D structure databases
ProteinModelPortal	P53262.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53262. 6 interactions.
MINT	MINT-4487272.
STRING	P53262.
Proteomic databases
PeptideAtlas	P53262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR106C; YGR106C; YGR106C.
GeneID	852998.
KEGG	sce:YGR106C.
NMPDR	fig|4932.3.peg.2737.
Organism-specific databases
CYGD	YGR106c.
SGD	S000003338. VOA1.
Phylogenomic databases
eggNOG	fuNOG12741.
OMA	PENSHED.
OrthoDB	EOG47H90T.
Gene expression databases
ArrayExpress	P53262.
Genevestigator	P53262.
GermOnline	YGR106C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	972839.

Entry information

Entry name

VOA1_YEAST

Accession

Primary (citable) accession number: P53262
Secondary accession number(s): D6VUN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program