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Protein

Pescadillo homolog

Gene

NOP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation4 Publications

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: GO_Central
  • ribonucleoprotein complex binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30813-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pescadillo homologUniRule annotation
Alternative name(s):
Nucleolar protein 7
Ribosomal RNA-processing protein 13
Gene namesi
Name:NOP7UniRule annotation
Synonyms:RRP13, YPH1
Ordered Locus Names:YGR103W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR103w.
EuPathDBiFungiDB:YGR103W.
SGDiS000003335. NOP7.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • PeBoW complex Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi380 – 3801I → R: Temperature sensitive mutant. 1 Publication
Mutagenesisi431 – 4311W → R: Temperature sensitive mutant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Pescadillo homologPRO_0000186192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei288 – 2881Phosphoserine1 Publication
Modified residuei308 – 3081Phosphothreonine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53261.
PaxDbiP53261.
PeptideAtlasiP53261.

Expressioni

Inductioni

Expression is down-regulated prior to the diauxic shift.1 Publication

Interactioni

Subunit structurei

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1. May also associate with the origin recognition complex (ORC complex).UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIC1P387794EBI-13145,EBI-24538
ERB1Q046609EBI-13145,EBI-28098
NOC2P397443EBI-13145,EBI-29259
NOG1Q028924EBI-13145,EBI-12105

Protein-protein interaction databases

BioGridi33346. 146 interactions.
DIPiDIP-6326N.
IntActiP53261. 79 interactions.
MINTiMINT-8285233.

Structurei

3D structure databases

ProteinModelPortaliP53261.
SMRiP53261. Positions 359-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini355 – 44995BRCTUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 484434Sufficient for interaction with ERB1Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili294 – 34249UniRule annotationAdd
BLAST
Coiled coili530 – 60576UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pescadillo family.UniRule annotation
Contains 1 BRCT domain.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5163.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
InParanoidiP53261.
KOiK14843.
OMAiIEAMQIS.
OrthoDBiEOG7HHX2C.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK
60 70 80 90 100
KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS
110 120 130 140 150
SAKRLEENRD SYTLDHIIKE RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ
160 170 180 190 200
VSSKIINDAQ KICNQWLAYV AKERLVRKVF VSIKGVYYQA NIKGEEVRWL
210 220 230 240 250
VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS GLIYPPKLDL
260 270 280 290 300
KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA
310 320 330 340 350
DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS
360 370 380 390 400
KYDSPVASLF SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK
410 420 430 440 450
DIDMSKVTHQ IVDRPVLKNK VAGRTYIQPQ WIFDCINKGE LVPANKYLPG
460 470 480 490 500
EALPPHLSPW GDAIGYDPTA PVEEGEEEES ESESESEDQV EEEDQEVVAG
510 520 530 540 550
EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN KKRKVDEEEE
560 570 580 590 600
EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK

LDSKK
Length:605
Mass (Da):69,877
Last modified:October 1, 1996 - v1
Checksum:i131001C956787BE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72888 Genomic DNA. Translation: CAA97106.1.
AY693015 Genomic DNA. Translation: AAT93034.1.
BK006941 Genomic DNA. Translation: DAA08196.1.
PIRiS64410.
RefSeqiNP_011617.1. NM_001181232.1.

Genome annotation databases

EnsemblFungiiYGR103W; YGR103W; YGR103W.
GeneIDi852995.
KEGGisce:YGR103W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72888 Genomic DNA. Translation: CAA97106.1.
AY693015 Genomic DNA. Translation: AAT93034.1.
BK006941 Genomic DNA. Translation: DAA08196.1.
PIRiS64410.
RefSeqiNP_011617.1. NM_001181232.1.

3D structure databases

ProteinModelPortaliP53261.
SMRiP53261. Positions 359-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33346. 146 interactions.
DIPiDIP-6326N.
IntActiP53261. 79 interactions.
MINTiMINT-8285233.

Proteomic databases

MaxQBiP53261.
PaxDbiP53261.
PeptideAtlasiP53261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR103W; YGR103W; YGR103W.
GeneIDi852995.
KEGGisce:YGR103W.

Organism-specific databases

CYGDiYGR103w.
EuPathDBiFungiDB:YGR103W.
SGDiS000003335. NOP7.

Phylogenomic databases

eggNOGiCOG5163.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
InParanoidiP53261.
KOiK14843.
OMAiIEAMQIS.
OrthoDBiEOG7HHX2C.

Enzyme and pathway databases

BioCyciYEAST:G3O-30813-MONOMER.

Miscellaneous databases

NextBioi972830.
PROiP53261.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis."
    Du Y.-C.N., Stillman B.
    Cell 109:835-848(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION WITH THE ORC COMPLEX, SUBCELLULAR LOCATION, DELETION PHENOTYPE, INDUCTION, MUTAGENESIS OF ILE-380 AND TRP-431.
  5. "Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits."
    Adams C.C., Jakovljevic J., Roman J., Harnpicharnchai P., Woolford J.L. Jr.
    RNA 8:150-165(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  6. "Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis."
    Oeffinger M., Leung A., Lamond A., Tollervey D.
    RNA 8:626-636(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Erratum
    Oeffinger M., Leung A., Lamond A., Tollervey D.
    RNA 8:851-851(2002)
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes."
    Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr.
    Mol. Cell. Biol. 25:10419-10432(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NOP7 COMPLEX WITH ERB1 AND YTM1, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
  10. "TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients."
    Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T.
    EMBO J. 25:3832-3842(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOG1, SUBCELLULAR LOCATION.
  11. "Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes."
    Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr.
    Mol. Biol. Cell 19:2844-2856(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288 AND THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPESC_YEAST
AccessioniPrimary (citable) accession number: P53261
Secondary accession number(s): D6VUN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4530 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.