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P53261

- PESC_YEAST

UniProt

P53261 - PESC_YEAST

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Protein

Pescadillo homolog

Gene
NOP7, RRP13, YPH1, YGR103W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.4 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. ribonucleoprotein complex binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle DNA replication Source: SGD
  2. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
  3. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
  4. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  5. ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30813-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pescadillo homolog
Alternative name(s):
Nucleolar protein 7
Ribosomal RNA-processing protein 13
Gene namesi
Name:NOP7
Synonyms:RRP13, YPH1
Ordered Locus Names:YGR103W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR103w.
SGDiS000003335. NOP7.

Subcellular locationi

Nucleusnucleolus. Nucleusnucleoplasm
Note: Accumulates in the nucleolus in response to rapamycin treatment.5 Publications

GO - Cellular componenti

  1. nucleolus Source: SGD
  2. nucleoplasm Source: UniProtKB-SubCell
  3. nucleus Source: SGD
  4. PeBoW complex Source: SGD
  5. preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi380 – 3801I → R: Temperature sensitive mutant. 1 Publication
Mutagenesisi431 – 4311W → R: Temperature sensitive mutant. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Pescadillo homologUniRule annotationPRO_0000186192Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei288 – 2881Phosphoserine1 Publication
Modified residuei308 – 3081Phosphothreonine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53261.
PaxDbiP53261.
PeptideAtlasiP53261.

Expressioni

Inductioni

Expression is down-regulated prior to the diauxic shift.1 Publication

Gene expression databases

GenevestigatoriP53261.

Interactioni

Subunit structurei

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1. May also associate with the origin recognition complex (ORC complex).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CIC1P387794EBI-13145,EBI-24538
NOC2P397443EBI-13145,EBI-29259
NOG1Q028924EBI-13145,EBI-12105

Protein-protein interaction databases

BioGridi33346. 148 interactions.
DIPiDIP-6326N.
IntActiP53261. 79 interactions.
MINTiMINT-8285233.
STRINGi4932.YGR103W.

Structurei

3D structure databases

ProteinModelPortaliP53261.
SMRiP53261. Positions 359-448.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini355 – 44995BRCTAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 484434Sufficient for interaction with ERB1UniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili294 – 34249 Reviewed predictionAdd
BLAST
Coiled coili530 – 60576 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the pescadillo family.
Contains 1 BRCT domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5163.
GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
KOiK14843.
OMAiADEANTD.
OrthoDBiEOG7HHX2C.

Family and domain databases

Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo.
InterProiIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53261-1 [UniParc]FASTAAdd to Basket

« Hide

MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK    50
KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS 100
SAKRLEENRD SYTLDHIIKE RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ 150
VSSKIINDAQ KICNQWLAYV AKERLVRKVF VSIKGVYYQA NIKGEEVRWL 200
VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS GLIYPPKLDL 250
KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA 300
DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS 350
KYDSPVASLF SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK 400
DIDMSKVTHQ IVDRPVLKNK VAGRTYIQPQ WIFDCINKGE LVPANKYLPG 450
EALPPHLSPW GDAIGYDPTA PVEEGEEEES ESESESEDQV EEEDQEVVAG 500
EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN KKRKVDEEEE 550
EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK 600
LDSKK 605
Length:605
Mass (Da):69,877
Last modified:October 1, 1996 - v1
Checksum:i131001C956787BE5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72888 Genomic DNA. Translation: CAA97106.1.
AY693015 Genomic DNA. Translation: AAT93034.1.
BK006941 Genomic DNA. Translation: DAA08196.1.
PIRiS64410.
RefSeqiNP_011617.1. NM_001181232.1.

Genome annotation databases

EnsemblFungiiYGR103W; YGR103W; YGR103W.
GeneIDi852995.
KEGGisce:YGR103W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72888 Genomic DNA. Translation: CAA97106.1 .
AY693015 Genomic DNA. Translation: AAT93034.1 .
BK006941 Genomic DNA. Translation: DAA08196.1 .
PIRi S64410.
RefSeqi NP_011617.1. NM_001181232.1.

3D structure databases

ProteinModelPortali P53261.
SMRi P53261. Positions 359-448.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33346. 148 interactions.
DIPi DIP-6326N.
IntActi P53261. 79 interactions.
MINTi MINT-8285233.
STRINGi 4932.YGR103W.

Proteomic databases

MaxQBi P53261.
PaxDbi P53261.
PeptideAtlasi P53261.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR103W ; YGR103W ; YGR103W .
GeneIDi 852995.
KEGGi sce:YGR103W.

Organism-specific databases

CYGDi YGR103w.
SGDi S000003335. NOP7.

Phylogenomic databases

eggNOGi COG5163.
GeneTreei ENSGT00390000002626.
HOGENOMi HOG000006022.
KOi K14843.
OMAi ADEANTD.
OrthoDBi EOG7HHX2C.

Enzyme and pathway databases

BioCyci YEAST:G3O-30813-MONOMER.

Miscellaneous databases

NextBioi 972830.
PROi P53261.

Gene expression databases

Genevestigatori P53261.

Family and domain databases

Gene3Di 3.40.50.10190. 1 hit.
HAMAPi MF_03028. Pescadillo.
InterProi IPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 1 hit.
[Graphical view ]
SUPFAMi SSF52113. SSF52113. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis."
    Du Y.-C.N., Stillman B.
    Cell 109:835-848(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION WITH THE ORC COMPLEX, SUBCELLULAR LOCATION, DELETION PHENOTYPE, INDUCTION, MUTAGENESIS OF ILE-380 AND TRP-431.
  5. "Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits."
    Adams C.C., Jakovljevic J., Roman J., Harnpicharnchai P., Woolford J.L. Jr.
    RNA 8:150-165(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  6. "Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis."
    Oeffinger M., Leung A., Lamond A., Tollervey D.
    RNA 8:626-636(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Erratum
    Oeffinger M., Leung A., Lamond A., Tollervey D.
    RNA 8:851-851(2002)
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes."
    Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr.
    Mol. Cell. Biol. 25:10419-10432(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NOP7 COMPLEX WITH ERB1 AND YTM1, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
  10. "TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients."
    Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T.
    EMBO J. 25:3832-3842(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOG1, SUBCELLULAR LOCATION.
  11. "Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes."
    Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr.
    Mol. Biol. Cell 19:2844-2856(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288 AND THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPESC_YEAST
AccessioniPrimary (citable) accession number: P53261
Secondary accession number(s): D6VUN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4530 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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