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P53261 (PESC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pescadillo homolog
Alternative name(s):
Nucleolar protein 7
Ribosomal RNA-processing protein 13
Gene names
Name:NOP7
Synonyms:RRP13, YPH1
Ordered Locus Names:YGR103W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.4 Ref.5 Ref.6 Ref.11

Subunit structure

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1. May also associate with the origin recognition complex (ORC complex). Ref.4 Ref.9 Ref.10

Subcellular location

Nucleusnucleolus. Nucleusnucleoplasm. Note: Accumulates in the nucleolus in response to rapamycin treatment. Ref.4 Ref.5 Ref.6 Ref.10 Ref.11

Induction

Expression is down-regulated prior to the diauxic shift. Ref.4

Disruption phenotype

Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes. Ref.5

Miscellaneous

Present with 4530 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the pescadillo family.

Contains 1 BRCT domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Pescadillo homolog HAMAP-Rule MF_03028
PRO_0000186192

Regions

Domain355 – 44995BRCT
Region51 – 484434Sufficient for interaction with ERB1 HAMAP-Rule MF_03028
Coiled coil294 – 34249 Potential
Coiled coil530 – 60576 Potential

Amino acid modifications

Modified residue2881Phosphoserine Ref.12
Modified residue3081Phosphothreonine Ref.12 Ref.13

Experimental info

Mutagenesis3801I → R: Temperature sensitive mutant. Ref.4
Mutagenesis4311W → R: Temperature sensitive mutant. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P53261 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 131001C956787BE5

FASTA60569,877
        10         20         30         40         50         60 
MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT 

        70         80         90        100        110        120 
TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS SAKRLEENRD SYTLDHIIKE 

       130        140        150        160        170        180 
RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ VSSKIINDAQ KICNQWLAYV AKERLVRKVF 

       190        200        210        220        230        240 
VSIKGVYYQA NIKGEEVRWL VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS 

       250        260        270        280        290        300 
GLIYPPKLDL KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA 

       310        320        330        340        350        360 
DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS KYDSPVASLF 

       370        380        390        400        410        420 
SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK DIDMSKVTHQ IVDRPVLKNK 

       430        440        450        460        470        480 
VAGRTYIQPQ WIFDCINKGE LVPANKYLPG EALPPHLSPW GDAIGYDPTA PVEEGEEEES 

       490        500        510        520        530        540 
ESESESEDQV EEEDQEVVAG EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN 

       550        560        570        580        590        600 
KKRKVDEEEE EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK 


LDSKK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis."
Du Y.-C.N., Stillman B.
Cell 109:835-848(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION WITH THE ORC COMPLEX, SUBCELLULAR LOCATION, DELETION PHENOTYPE, INDUCTION, MUTAGENESIS OF ILE-380 AND TRP-431.
[5]"Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits."
Adams C.C., Jakovljevic J., Roman J., Harnpicharnchai P., Woolford J.L. Jr.
RNA 8:150-165(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]"Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis."
Oeffinger M., Leung A., Lamond A., Tollervey D.
RNA 8:626-636(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]Erratum
Oeffinger M., Leung A., Lamond A., Tollervey D.
RNA 8:851-851(2002)
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes."
Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr.
Mol. Cell. Biol. 25:10419-10432(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE NOP7 COMPLEX WITH ERB1 AND YTM1, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
[10]"TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients."
Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T.
EMBO J. 25:3832-3842(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NOG1, SUBCELLULAR LOCATION.
[11]"Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes."
Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr.
Mol. Biol. Cell 19:2844-2856(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288 AND THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72888 Genomic DNA. Translation: CAA97106.1.
AY693015 Genomic DNA. Translation: AAT93034.1.
BK006941 Genomic DNA. Translation: DAA08196.1.
PIRS64410.
RefSeqNP_011617.1. NM_001181232.1.

3D structure databases

ProteinModelPortalP53261.
SMRP53261. Positions 359-448.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33346. 148 interactions.
DIPDIP-6326N.
IntActP53261. 79 interactions.
MINTMINT-8285233.
STRING4932.YGR103W.

Proteomic databases

PaxDbP53261.
PeptideAtlasP53261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR103W; YGR103W; YGR103W.
GeneID852995.
KEGGsce:YGR103W.

Organism-specific databases

CYGDYGR103w.
SGDS000003335. NOP7.

Phylogenomic databases

eggNOGCOG5163.
GeneTreeENSGT00390000002626.
HOGENOMHOG000006022.
KOK14843.
OMAEEQRMEG.
OrthoDBEOG7HHX2C.

Enzyme and pathway databases

BioCycYEAST:G3O-30813-MONOMER.

Gene expression databases

GenevestigatorP53261.

Family and domain databases

Gene3D3.40.50.10190. 1 hit.
HAMAPMF_03028. Pescadillo.
InterProIPR001357. BRCT_dom.
IPR010613. PES.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF06732. Pescadillo_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 1 hit.
[Graphical view]
SUPFAMSSF52113. SSF52113. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972830.
PROP53261.

Entry information

Entry namePESC_YEAST
AccessionPrimary (citable) accession number: P53261
Secondary accession number(s): D6VUN5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families