P53261 (PESC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pescadillo homolog

Alternative name(s):
Nucleolar protein 7
Ribosomal RNA-processing protein 13

Gene names

Name:	NOP7
Synonyms:	RRP13, YPH1
Ordered Locus Names:	YGR103W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Ref.4 Ref.5 Ref.6 Ref.12
Subunit structure	Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1. May also associate with the origin recognition complex (ORC complex). Ref.10
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm. Note: Accumulates in the nucleolus in response to rapamycin treatment. Ref.4 Ref.5 Ref.6 Ref.10 Ref.12
Induction	Expression is down-regulated prior to the diauxic shift. Ref.4
Disruption phenotype	Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes. Ref.5
Miscellaneous	Present with 4530 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the pescadillo family. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from mutant phenotype Ref.4. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD ribosomal large subunit biogenesis Inferred from direct assay Ref.5Ref.10. Source: SGD
Cellular component	PeBoW complex Inferred from direct assay Ref.9. Source: SGD nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell preribosome, large subunit precursor Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CIC1	P38779	4	EBI-13145,EBI-24538
NOC2	P39744	3	EBI-13145,EBI-29259

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Pescadillo homolog		PRO_0000186192
Regions
	Domain	355 – 449	95	BRCT
	Region	51 – 484	434	Sufficient for interaction with ERB1
	Coiled coil	294 – 342	49	Potential
Amino acid modifications
	Modified residue	261	1	Phosphoserine Ref.13
	Modified residue	266	1	Phosphoserine Ref.13
	Modified residue	288	1	Phosphoserine Ref.13
	Modified residue	305	1	Phosphothreonine Ref.13
	Modified residue	308	1	Phosphothreonine Ref.11 Ref.13
	Modified residue	334	1	Phosphothreonine Ref.13
	Modified residue	529	1	Phosphoserine Ref.11 Ref.13
Experimental info
	Mutagenesis	380	1	I → R: Temperature sensitive mutant. Ref.4
	Mutagenesis	431	1	W → R: Temperature sensitive mutant. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P53261 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 131001C956787BE5	FASTA	605	69,877
	10 20 30 40 50 60 MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT 70 80 90 100 110 120 TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS SAKRLEENRD SYTLDHIIKE 130 140 150 160 170 180 RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ VSSKIINDAQ KICNQWLAYV AKERLVRKVF 190 200 210 220 230 240 VSIKGVYYQA NIKGEEVRWL VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS 250 260 270 280 290 300 GLIYPPKLDL KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA 310 320 330 340 350 360 DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS KYDSPVASLF 370 380 390 400 410 420 SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK DIDMSKVTHQ IVDRPVLKNK 430 440 450 460 470 480 VAGRTYIQPQ WIFDCINKGE LVPANKYLPG EALPPHLSPW GDAIGYDPTA PVEEGEEEES 490 500 510 520 530 540 ESESESEDQV EEEDQEVVAG EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN 550 560 570 580 590 600 KKRKVDEEEE EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK LDSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis." Du Y.-C.N., Stillman B. Cell 109:835-848(2002) [PubMed: 12110181] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION WITH THE ORC COMPLEX, SUBCELLULAR LOCATION, DELETION PHENOTYPE, INDUCTION, MUTAGENESIS OF ILE-380 AND TRP-431.
[5]	"Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits." Adams C.C., Jakovljevic J., Roman J., Harnpicharnchai P., Woolford J.L. Jr. RNA 8:150-165(2002) [PubMed: 11911362] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]	"Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis." Oeffinger M., Leung A., Lamond A., Tollervey D. RNA 8:626-636(2002) [PubMed: 12022229] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	Erratum Oeffinger M., Leung A., Lamond A., Tollervey D. RNA 8:851-851(2002)
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes." Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr. Mol. Cell. Biol. 25:10419-10432(2005) [PubMed: 16287855] [Abstract] Cited for: IDENTIFICATION IN THE NOP7 COMPLEX WITH ERB1 AND YTM1, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
[10]	"TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients." Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T. EMBO J. 25:3832-3842(2006) [PubMed: 16888624] [Abstract] Cited for: INTERACTION WITH NOG1, SUBCELLULAR LOCATION.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-308 AND SER-529, MASS SPECTROMETRY.
[12]	"Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes." Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr. Mol. Biol. Cell 19:2844-2856(2008) [PubMed: 18448671] [Abstract] Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261; SER-266; SER-288; THR-305; THR-308; THR-334 AND SER-529, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72888 Genomic DNA. Translation: CAA97106.1. AY693015 Genomic DNA. Translation: AAT93034.1. BK006941 Genomic DNA. Translation: DAA08196.1.
PIR	S64410.
RefSeq	NP_011617.1. NM_001181232.1.
3D structure databases
ProteinModelPortal	P53261.
SMR	P53261. Positions 351-448.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6326N.
IntAct	P53261. 93 interactions.
MINT	MINT-633106.
STRING	P53261.
Proteomic databases
PeptideAtlas	P53261.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR103W; YGR103W; YGR103W.
GeneID	852995.
KEGG	sce:YGR103W.
NMPDR	fig|4932.3.peg.2734.
Organism-specific databases
CYGD	YGR103w.
SGD	S000003335. NOP7.
Phylogenomic databases
eggNOG	fuNOG04161.
GeneTree	EFGT00050000001564.
HOGENOM	HBG410102.
OMA	SIKGVYY.
OrthoDB	EOG49S9FS.
Gene expression databases
ArrayExpress	P53261.
Genevestigator	P53261.
GermOnline	YGR103W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001357. BRCT. IPR010613. Pescadillo. [Graphical view]
KO	K14843.
Pfam	PF00533. BRCT. 1 hit. PF06732. Pescadillo_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972830.

Entry information

Entry name

PESC_YEAST

Accession

Primary (citable) accession number: P53261
Secondary accession number(s): D6VUN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program