Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53261

- PESC_YEAST

UniProt

P53261 - PESC_YEAST

Protein

Pescadillo homolog

Gene

NOP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.4 PublicationsUniRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ribonucleoprotein complex binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell cycle DNA replication Source: SGD
    2. maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
    3. maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: UniProtKB-HAMAP
    4. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    5. ribosomal large subunit biogenesis Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30813-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pescadillo homologUniRule annotation
    Alternative name(s):
    Nucleolar protein 7
    Ribosomal RNA-processing protein 13
    Gene namesi
    Name:NOP7UniRule annotation
    Synonyms:RRP13, YPH1
    Ordered Locus Names:YGR103W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR103w.
    SGDiS000003335. NOP7.

    Subcellular locationi

    Nucleusnucleolus. Nucleusnucleoplasm
    Note: Accumulates in the nucleolus in response to rapamycin treatment.

    GO - Cellular componenti

    1. nucleolus Source: SGD
    2. nucleoplasm Source: UniProtKB-SubCell
    3. nucleus Source: SGD
    4. PeBoW complex Source: SGD
    5. preribosome, large subunit precursor Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi380 – 3801I → R: Temperature sensitive mutant. 1 Publication
    Mutagenesisi431 – 4311W → R: Temperature sensitive mutant. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 605605Pescadillo homologPRO_0000186192Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei288 – 2881Phosphoserine1 Publication
    Modified residuei308 – 3081Phosphothreonine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53261.
    PaxDbiP53261.
    PeptideAtlasiP53261.

    Expressioni

    Inductioni

    Expression is down-regulated prior to the diauxic shift.1 Publication

    Gene expression databases

    GenevestigatoriP53261.

    Interactioni

    Subunit structurei

    Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome. Also interacts with NOG1. May also associate with the origin recognition complex (ORC complex).3 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CIC1P387794EBI-13145,EBI-24538
    NOC2P397443EBI-13145,EBI-29259
    NOG1Q028924EBI-13145,EBI-12105

    Protein-protein interaction databases

    BioGridi33346. 148 interactions.
    DIPiDIP-6326N.
    IntActiP53261. 79 interactions.
    MINTiMINT-8285233.
    STRINGi4932.YGR103W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53261.
    SMRiP53261. Positions 359-448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini355 – 44995BRCTUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 484434Sufficient for interaction with ERB1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili294 – 34249UniRule annotationAdd
    BLAST
    Coiled coili530 – 60576UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the pescadillo family.UniRule annotation
    Contains 1 BRCT domain.UniRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5163.
    GeneTreeiENSGT00390000002626.
    HOGENOMiHOG000006022.
    KOiK14843.
    OMAiADEANTD.
    OrthoDBiEOG7HHX2C.

    Family and domain databases

    Gene3Di3.40.50.10190. 1 hit.
    HAMAPiMF_03028. Pescadillo.
    InterProiIPR001357. BRCT_dom.
    IPR010613. PES.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF06732. Pescadillo_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 1 hit.
    [Graphical view]
    SUPFAMiSSF52113. SSF52113. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53261-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK    50
    KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS 100
    SAKRLEENRD SYTLDHIIKE RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ 150
    VSSKIINDAQ KICNQWLAYV AKERLVRKVF VSIKGVYYQA NIKGEEVRWL 200
    VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS GLIYPPKLDL 250
    KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA 300
    DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS 350
    KYDSPVASLF SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK 400
    DIDMSKVTHQ IVDRPVLKNK VAGRTYIQPQ WIFDCINKGE LVPANKYLPG 450
    EALPPHLSPW GDAIGYDPTA PVEEGEEEES ESESESEDQV EEEDQEVVAG 500
    EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN KKRKVDEEEE 550
    EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK 600
    LDSKK 605
    Length:605
    Mass (Da):69,877
    Last modified:October 1, 1996 - v1
    Checksum:i131001C956787BE5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72888 Genomic DNA. Translation: CAA97106.1.
    AY693015 Genomic DNA. Translation: AAT93034.1.
    BK006941 Genomic DNA. Translation: DAA08196.1.
    PIRiS64410.
    RefSeqiNP_011617.1. NM_001181232.1.

    Genome annotation databases

    EnsemblFungiiYGR103W; YGR103W; YGR103W.
    GeneIDi852995.
    KEGGisce:YGR103W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72888 Genomic DNA. Translation: CAA97106.1 .
    AY693015 Genomic DNA. Translation: AAT93034.1 .
    BK006941 Genomic DNA. Translation: DAA08196.1 .
    PIRi S64410.
    RefSeqi NP_011617.1. NM_001181232.1.

    3D structure databases

    ProteinModelPortali P53261.
    SMRi P53261. Positions 359-448.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33346. 148 interactions.
    DIPi DIP-6326N.
    IntActi P53261. 79 interactions.
    MINTi MINT-8285233.
    STRINGi 4932.YGR103W.

    Proteomic databases

    MaxQBi P53261.
    PaxDbi P53261.
    PeptideAtlasi P53261.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR103W ; YGR103W ; YGR103W .
    GeneIDi 852995.
    KEGGi sce:YGR103W.

    Organism-specific databases

    CYGDi YGR103w.
    SGDi S000003335. NOP7.

    Phylogenomic databases

    eggNOGi COG5163.
    GeneTreei ENSGT00390000002626.
    HOGENOMi HOG000006022.
    KOi K14843.
    OMAi ADEANTD.
    OrthoDBi EOG7HHX2C.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30813-MONOMER.

    Miscellaneous databases

    NextBioi 972830.
    PROi P53261.

    Gene expression databases

    Genevestigatori P53261.

    Family and domain databases

    Gene3Di 3.40.50.10190. 1 hit.
    HAMAPi MF_03028. Pescadillo.
    InterProi IPR001357. BRCT_dom.
    IPR010613. PES.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF06732. Pescadillo_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52113. SSF52113. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis."
      Du Y.-C.N., Stillman B.
      Cell 109:835-848(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NOP7 COMPLEX, ASSOCIATION WITH THE ORC COMPLEX, SUBCELLULAR LOCATION, DELETION PHENOTYPE, INDUCTION, MUTAGENESIS OF ILE-380 AND TRP-431.
    5. "Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits."
      Adams C.C., Jakovljevic J., Roman J., Harnpicharnchai P., Woolford J.L. Jr.
      RNA 8:150-165(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    6. "Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis."
      Oeffinger M., Leung A., Lamond A., Tollervey D.
      RNA 8:626-636(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. Erratum
      Oeffinger M., Leung A., Lamond A., Tollervey D.
      RNA 8:851-851(2002)
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes."
      Miles T.D., Jakovljevic J., Horsey E.W., Harnpicharnchai P., Tang L., Woolford J.L. Jr.
      Mol. Cell. Biol. 25:10419-10432(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NOP7 COMPLEX WITH ERB1 AND YTM1, ASSOCIATION OF THE NOP7 COMPLEX WITH 66S PRE-RIBOSOMES.
    10. "TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients."
      Honma Y., Kitamura A., Shioda R., Maruyama H., Ozaki K., Oda Y., Mini T., Jenoe P., Maki Y., Yonezawa K., Hurt E., Ueno M., Uritani M., Hall M.N., Ushimaru T.
      EMBO J. 25:3832-3842(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOG1, SUBCELLULAR LOCATION.
    11. "Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes."
      Tang L., Sahasranaman A., Jakovljevic J., Schleifman E., Woolford J.L. Jr.
      Mol. Biol. Cell 19:2844-2856(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CHARACTERIZATION OF THE NOP7 COMPLEX, SUBCELLULAR LOCATION.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288 AND THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPESC_YEAST
    AccessioniPrimary (citable) accession number: P53261
    Secondary accession number(s): D6VUN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4530 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3