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Protein

Pescadillo homolog

Gene

NOP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation4 Publications

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: GO_Central

GO - Biological processi

  • cell cycle DNA replication Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • regulation of rRNA processing Source: EnsemblPlants
  • ribosomal large subunit biogenesis Source: SGD

Keywordsi

Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30813-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pescadillo homolog1 PublicationUniRule annotation
Alternative name(s):
Nucleolar protein 71 Publication
Ribosomal RNA-processing protein 13
Gene namesi
Name:NOP71 PublicationUniRule annotation
Synonyms:RRP13, YPH11 Publication
Ordered Locus Names:YGR103WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR103W.
SGDiS000003335. NOP7.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: SGD
  • PeBoW complex Source: SGD
  • preribosome, large subunit precursor Source: SGD

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi380I → R: Temperature sensitive mutant. 1 Publication1
Mutagenesisi431W → R: Temperature sensitive mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001861921 – 605Pescadillo homologAdd BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei288PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53261.
PRIDEiP53261.

PTM databases

iPTMnetiP53261.

Expressioni

Inductioni

Expression is down-regulated prior to the diauxic shift.1 Publication

Interactioni

Subunit structurei

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. The complex is held together by ERB1, which interacts with NOP7 via its N-terminal domain and with YTM1 via a high-affinity interaction between the seven-bladed beta-propeller domains of the 2 proteins. The NOP7 complex associates with the 66S pre-ribosome (PubMed:16287855). Also interacts with NOG1 (PubMed:16888624). May also associate with the origin recognition complex (ORC complex) (PubMed:12110181).UniRule annotation3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33346. 224 interactors.
DIPiDIP-6326N.
IntActiP53261. 81 interactors.
MINTiMINT-8285233.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08n1-605[»]
ProteinModelPortaliP53261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini355 – 449BRCTUniRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 484Sufficient for interaction with ERB11 PublicationAdd BLAST434

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili294 – 342UniRule annotationAdd BLAST49
Coiled coili530 – 605UniRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the pescadillo family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
InParanoidiP53261.
KOiK14843.
OMAiHDVDFRI.
OrthoDBiEOG092C1M1S.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo. 1 hit.
InterProiView protein in InterPro
IPR001357. BRCT_dom.
IPR010613. PES.
PANTHERiPTHR12221. PTHR12221. 1 hit.
PfamiView protein in Pfam
PF16589. BRCT_2. 1 hit.
PF06732. Pescadillo_N. 1 hit.
SMARTiView protein in SMART
SM00292. BRCT. 1 hit.
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiView protein in PROSITE
PS50172. BRCT. 1 hit.

Sequencei

Sequence statusi: Complete.

P53261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK
60 70 80 90 100
KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS
110 120 130 140 150
SAKRLEENRD SYTLDHIIKE RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ
160 170 180 190 200
VSSKIINDAQ KICNQWLAYV AKERLVRKVF VSIKGVYYQA NIKGEEVRWL
210 220 230 240 250
VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS GLIYPPKLDL
260 270 280 290 300
KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA
310 320 330 340 350
DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS
360 370 380 390 400
KYDSPVASLF SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK
410 420 430 440 450
DIDMSKVTHQ IVDRPVLKNK VAGRTYIQPQ WIFDCINKGE LVPANKYLPG
460 470 480 490 500
EALPPHLSPW GDAIGYDPTA PVEEGEEEES ESESESEDQV EEEDQEVVAG
510 520 530 540 550
EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN KKRKVDEEEE
560 570 580 590 600
EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK

LDSKK
Length:605
Mass (Da):69,877
Last modified:October 1, 1996 - v1
Checksum:i131001C956787BE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72888 Genomic DNA. Translation: CAA97106.1.
AY693015 Genomic DNA. Translation: AAT93034.1.
BK006941 Genomic DNA. Translation: DAA08196.1.
PIRiS64410.
RefSeqiNP_011617.1. NM_001181232.1.

Genome annotation databases

EnsemblFungiiYGR103W; YGR103W; YGR103W.
GeneIDi852995.
KEGGisce:YGR103W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72888 Genomic DNA. Translation: CAA97106.1.
AY693015 Genomic DNA. Translation: AAT93034.1.
BK006941 Genomic DNA. Translation: DAA08196.1.
PIRiS64410.
RefSeqiNP_011617.1. NM_001181232.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08n1-605[»]
ProteinModelPortaliP53261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33346. 224 interactors.
DIPiDIP-6326N.
IntActiP53261. 81 interactors.
MINTiMINT-8285233.

PTM databases

iPTMnetiP53261.

Proteomic databases

MaxQBiP53261.
PRIDEiP53261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR103W; YGR103W; YGR103W.
GeneIDi852995.
KEGGisce:YGR103W.

Organism-specific databases

EuPathDBiFungiDB:YGR103W.
SGDiS000003335. NOP7.

Phylogenomic databases

GeneTreeiENSGT00390000002626.
HOGENOMiHOG000006022.
InParanoidiP53261.
KOiK14843.
OMAiHDVDFRI.
OrthoDBiEOG092C1M1S.

Enzyme and pathway databases

BioCyciYEAST:G3O-30813-MONOMER.

Miscellaneous databases

PROiP53261.

Family and domain databases

CDDicd00027. BRCT. 1 hit.
Gene3Di3.40.50.10190. 1 hit.
HAMAPiMF_03028. Pescadillo. 1 hit.
InterProiView protein in InterPro
IPR001357. BRCT_dom.
IPR010613. PES.
PANTHERiPTHR12221. PTHR12221. 1 hit.
PfamiView protein in Pfam
PF16589. BRCT_2. 1 hit.
PF06732. Pescadillo_N. 1 hit.
SMARTiView protein in SMART
SM00292. BRCT. 1 hit.
SUPFAMiSSF52113. SSF52113. 1 hit.
PROSITEiView protein in PROSITE
PS50172. BRCT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPESC_YEAST
AccessioniPrimary (citable) accession number: P53261
Secondary accession number(s): D6VUN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 15, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4530 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.