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Protein

Pescadillo homolog

Gene

NOP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.UniRule annotation4 Publications

Miscellaneous

Present with 4530 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • large ribosomal subunit rRNA binding Source: GO_Central

GO - Biological processi

  • cell cycle DNA replication Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • regulation of rRNA processing Source: EnsemblPlants
  • ribosomal large subunit biogenesis Source: SGD

Keywordsi

Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30813-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pescadillo homolog1 PublicationUniRule annotation
Alternative name(s):
Nucleolar protein 71 Publication
Ribosomal RNA-processing protein 13
Gene namesi
Name:NOP71 PublicationUniRule annotation
Synonyms:RRP13, YPH11 Publication
Ordered Locus Names:YGR103WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR103W
SGDiS000003335 NOP7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Essential gene. Reduced assembly of 60S ribosomes and accumulation of halfmer polyribosomes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi380I → R: Temperature sensitive mutant. 1 Publication1
Mutagenesisi431W → R: Temperature sensitive mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001861921 – 605Pescadillo homologAdd BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei288PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53261
PaxDbiP53261
PRIDEiP53261

PTM databases

iPTMnetiP53261

Expressioni

Inductioni

Expression is down-regulated prior to the diauxic shift.1 Publication

Interactioni

Subunit structurei

Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. The complex is held together by ERB1, which interacts with NOP7 via its N-terminal domain and with YTM1 via a high-affinity interaction between the seven-bladed beta-propeller domains of the 2 proteins. The NOP7 complex associates with the 66S pre-ribosome (PubMed:16287855). Also interacts with NOG1 (PubMed:16888624). May also associate with the origin recognition complex (ORC complex) (PubMed:12110181).UniRule annotation3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi33346, 228 interactors
DIPiDIP-6326N
IntActiP53261, 129 interactors
MINTiP53261
STRINGi4932.YGR103W

Structurei

Secondary structure

1605
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 25Combined sources8
Helixi29 – 39Combined sources11
Helixi65 – 71Combined sources7
Helixi75 – 96Combined sources22
Helixi99 – 107Combined sources9
Helixi115 – 117Combined sources3
Turni118 – 121Combined sources4
Helixi125 – 128Combined sources4
Turni129 – 131Combined sources3
Helixi132 – 143Combined sources12
Beta strandi149 – 151Combined sources3
Helixi153 – 172Combined sources20
Beta strandi178 – 181Combined sources4
Beta strandi186 – 190Combined sources5
Beta strandi192 – 195Combined sources4
Helixi217 – 238Combined sources22
Helixi250 – 255Combined sources6
Beta strandi258 – 262Combined sources5
Beta strandi354 – 356Combined sources3
Turni357 – 362Combined sources6
Beta strandi369 – 371Combined sources3
Turni374 – 376Combined sources3
Helixi377 – 383Combined sources7
Beta strandi409 – 411Combined sources3
Beta strandi422 – 426Combined sources5
Helixi430 – 436Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JCTelectron microscopy3.08n1-605[»]
6C0Felectron microscopy3.70n1-605[»]
6ELZelectron microscopy3.30n1-605[»]
6EM1electron microscopy3.60n1-605[»]
6EM3electron microscopy3.20n1-605[»]
6EM4electron microscopy4.10n1-605[»]
6EM5electron microscopy4.30n1-605[»]
ProteinModelPortaliP53261
SMRiP53261
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini355 – 449BRCTUniRule annotationAdd BLAST95

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 484Sufficient for interaction with ERB11 PublicationAdd BLAST434

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili294 – 342UniRule annotationAdd BLAST49
Coiled coili530 – 605UniRule annotationAdd BLAST76

Sequence similaritiesi

Belongs to the pescadillo family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000002626
HOGENOMiHOG000006022
InParanoidiP53261
KOiK14843
OMAiQWVFDCV
OrthoDBiEOG092C1M1S

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_03028 Pescadillo, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR010613 PES
PANTHERiPTHR12221 PTHR12221, 1 hit
PfamiView protein in Pfam
PF16589 BRCT_2, 1 hit
PF06732 Pescadillo_N, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SUPFAMiSSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit

Sequencei

Sequence statusi: Complete.

P53261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK
60 70 80 90 100
KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS
110 120 130 140 150
SAKRLEENRD SYTLDHIIKE RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ
160 170 180 190 200
VSSKIINDAQ KICNQWLAYV AKERLVRKVF VSIKGVYYQA NIKGEEVRWL
210 220 230 240 250
VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS GLIYPPKLDL
260 270 280 290 300
KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA
310 320 330 340 350
DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS
360 370 380 390 400
KYDSPVASLF SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK
410 420 430 440 450
DIDMSKVTHQ IVDRPVLKNK VAGRTYIQPQ WIFDCINKGE LVPANKYLPG
460 470 480 490 500
EALPPHLSPW GDAIGYDPTA PVEEGEEEES ESESESEDQV EEEDQEVVAG
510 520 530 540 550
EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN KKRKVDEEEE
560 570 580 590 600
EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK

LDSKK
Length:605
Mass (Da):69,877
Last modified:October 1, 1996 - v1
Checksum:i131001C956787BE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72888 Genomic DNA Translation: CAA97106.1
AY693015 Genomic DNA Translation: AAT93034.1
BK006941 Genomic DNA Translation: DAA08196.1
PIRiS64410
RefSeqiNP_011617.1, NM_001181232.1

Genome annotation databases

EnsemblFungiiYGR103W; YGR103W; YGR103W
GeneIDi852995
KEGGisce:YGR103W

Similar proteinsi

Entry informationi

Entry nameiPESC_YEAST
AccessioniPrimary (citable) accession number: P53261
Secondary accession number(s): D6VUN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health