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Protein

GTPase-activating protein GYP2

Gene

MDR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates specifically the GTPase activity of SEC4, YPT6 and YPT36. Inactivates YPT6 during recycling between the endosome and the Golgi compartments.3 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • GTPase activator activity Source: SGD
  • Rab GTPase binding Source: GO_Central

GO - Biological processi

  • activation of GTPase activity Source: GO_Central
  • intracellular protein transport Source: SGD
  • regulation of vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-30810-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase-activating protein GYP2
Alternative name(s):
MAC1-dependent regulator
Protein MIC1
Gene namesi
Name:MDR1
Synonyms:GYP2, MIC1
Ordered Locus Names:YGR100W
ORF Names:G5717
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR100W.
SGDiS000003332. MDR1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • endomembrane system Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 950949GTPase-activating protein GYP2PRO_0000208011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei764 – 7641PhosphoserineCombined sources
Modified residuei871 – 8711PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP53258.

PTM databases

iPTMnetiP53258.

Interactioni

Subunit structurei

Interacts with MAC1.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33343. 38 interactions.
DIPiDIP-4578N.
IntActiP53258. 4 interactions.
MINTiMINT-558675.

Structurei

3D structure databases

ProteinModelPortaliP53258.
SMRiP53258. Positions 209-517, 569-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 11688GRAMAdd
BLAST
Domaini244 – 432189Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST
Domaini602 – 63736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini638 – 67336EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 GRAM domain.Curated
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119137.
HOGENOMiHOG000173111.
InParanoidiP53258.
KOiK19951.
OMAiTITYFFE.
OrthoDBiEOG092C0Q84.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR004182. GRAM.
IPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF02893. GRAM. 1 hit.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47923. SSF47923. 2 hits.
PROSITEiPS50222. EF_HAND_2. 2 hits.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFFDSLRQK APFLDKLADS FTPTLTRDEK FRLKYKLPAN ENILEDTNAE
60 70 80 90 100
VSFATSIKDG KGHSDRVNNK GRKTAYVYSG RLFLTPHFLV FRDAFDHSSC
110 120 130 140 150
VLILNISTIK RVERSPSESY EFALLVTLYT GAKVLIQFIG IRYRSEQFCD
160 170 180 190 200
KLKLNLKENI PNAKTLPAFL ETSYSEFLIA KNILGKKDIT VPRAGLGQHF
210 220 230 240 250
KYPGNPTMVK EKAKLRLWFD YFRENGRNLA VVQTPMFRKL IRIGVPNRMR
260 270 280 290 300
GEIWELCSGA MYMRYANSGE YERILNENAG KTSQAIDEIE KDLKRSLPEY
310 320 330 340 350
SAYQTEEGIQ RLRNVLTAYS WKNPDVGYCQ AMNIVVAGFL IFMSEEQAFW
360 370 380 390 400
CLCNLCDIYV PGYYSKTMYG TLLDQRVFES FVEDRMPVLW EYILQHDIQL
410 420 430 440 450
SVVSLPWFLS LFFTSMPLEY AVRIMDIFFM NGSITLFQVA LAVLKINADD
460 470 480 490 500
ILQADDDGMF IAIIKHYFQT LGQSAHPDSS DIKYRQITKF QELLVTAFKE
510 520 530 540 550
FSVISEEMAM HARHKYEKGI FQNIETFMKR TQLRHMPKTF NLSSDDLSNI
560 570 580 590 600
YDMFYQSIET YKISMGTGSS NMGFEVFIQF LSKFCDSCRP CEKDKDPAFR
610 620 630 640 650
KQKRNFLQRL FDNWDSAHIG ELTLNDVVTG LDKLVTVDLL QAINYFFSLY
660 670 680 690 700
DTDGDGELHR EEVLQLSEGL LLLTEPWKSG RYVDLLTKKR IEDDIAENII
710 720 730 740 750
KESGGEIATM NQIELPTGVT IDEEKYKVEQ AERYLKAASN FLQRSFEYAK
760 770 780 790 800
AVDLAEEVNL IDLSDDEGEE KRTVKQKQLE SIKANAALDP THPKVIDLPT
810 820 830 840 850
FRMIILADET YELFFSNTLR SSVHVDEHVN IDNKNKVLRS MFDGILADGK
860 870 880 890 900
RVAEQVRRRV DSVATRSSIA SVESTPTAAA SSITTKEEKY DDLDDFTSEH
910 920 930 940 950
QPENEELLQS SWFEIDDANE TSTKAIQERS FEPLSANSSE EKSNLIEFEA
Length:950
Mass (Da):109,259
Last modified:October 1, 1996 - v1
Checksum:i888BC930C1358657
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52667 Genomic DNA. Translation: AAB01977.1.
Z72885 Genomic DNA. Translation: CAA97103.1.
BK006941 Genomic DNA. Translation: DAA08193.1.
PIRiS64405.
RefSeqiNP_011614.1. NM_001181229.1.

Genome annotation databases

EnsemblFungiiYGR100W; YGR100W; YGR100W.
GeneIDi852992.
KEGGisce:YGR100W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52667 Genomic DNA. Translation: AAB01977.1.
Z72885 Genomic DNA. Translation: CAA97103.1.
BK006941 Genomic DNA. Translation: DAA08193.1.
PIRiS64405.
RefSeqiNP_011614.1. NM_001181229.1.

3D structure databases

ProteinModelPortaliP53258.
SMRiP53258. Positions 209-517, 569-670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33343. 38 interactions.
DIPiDIP-4578N.
IntActiP53258. 4 interactions.
MINTiMINT-558675.

PTM databases

iPTMnetiP53258.

Proteomic databases

MaxQBiP53258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR100W; YGR100W; YGR100W.
GeneIDi852992.
KEGGisce:YGR100W.

Organism-specific databases

EuPathDBiFungiDB:YGR100W.
SGDiS000003332. MDR1.

Phylogenomic databases

GeneTreeiENSGT00760000119137.
HOGENOMiHOG000173111.
InParanoidiP53258.
KOiK19951.
OMAiTITYFFE.
OrthoDBiEOG092C0Q84.

Enzyme and pathway databases

BioCyciYEAST:G3O-30810-MONOMER.

Miscellaneous databases

PROiP53258.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR004182. GRAM.
IPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF02893. GRAM. 1 hit.
PF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47923. SSF47923. 2 hits.
PROSITEiPS50222. EF_HAND_2. 2 hits.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGYP2_YEAST
AccessioniPrimary (citable) accession number: P53258
Secondary accession number(s): D6VUN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.