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P53257

- TPC1_YEAST

UniProt

P53257 - TPC1_YEAST

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Protein

Mitochondrial thiamine pyrophosphate carrier 1

Gene

TPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.3 Publications

Kineticsi

  1. KM=0.51 mM for dATP uptake by unloaded proteolipososmes1 Publication

Vmax=38 µmol/min/g enzyme with dATP as substrate uptaken by unloaded proteolipososmes1 Publication

GO - Molecular functioni

  1. thiamine transmembrane transporter activity Source: SGD

GO - Biological processi

  1. thiamine pyrophosphate transport Source: SGD
  2. thiamine transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30806-MONOMER.

Protein family/group databases

TCDBi2.A.29.28.1. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial thiamine pyrophosphate carrier 1
Gene namesi
Name:TPC1
Ordered Locus Names:YGR096W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR096w.
SGDiS000003328. TPC1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314Mitochondrial thiamine pyrophosphate carrier 1PRO_0000090696Add
BLAST

Expressioni

Inductioni

Expression is regulated by carbon source, with very low expression with succinate, acetate, ethanol or pyruvate as carbon source.1 Publication

Gene expression databases

GenevestigatoriP53257.

Interactioni

Protein-protein interaction databases

BioGridi33339. 15 interactions.
IntActiP53257. 1 interaction.
MINTiMINT-4487206.
STRINGi4932.YGR096W.

Structurei

3D structure databases

ProteinModelPortaliP53257.
SMRiP53257. Positions 12-285.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3017Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei84 – 10017Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei116 – 13621Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei170 – 18617Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei217 – 23317Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei285 – 30218Helical; Name=6Sequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 10390Solcar 1Add
BLAST
Repeati110 – 19586Solcar 2Add
BLAST
Repeati210 – 310101Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG310116.
GeneTreeiENSGT00550000074902.
HOGENOMiHOG000165726.
InParanoidiP53257.
KOiK15108.
OMAiYETIRIY.
OrthoDBiEOG7N0CFV.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53257-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN
60 70 80 90 100
GLKPFGSQVM EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF
110 120 130 140 150
NRYLTPFGLE ARLHSLVVGA FAGITSSIVS YPFDVLRTRL VANNQMHSMS
160 170 180 190 200
ITREVRDIWK LEGLPGFFKG SIASMTTITL TASIMFGTYE TIRIYCDENE
210 220 230 240 250
KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM QFMNSKHLEK
260 270 280 290 300
FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS
310
FWGYETAIHY LRMY
Length:314
Mass (Da):35,137
Last modified:October 1, 1996 - v1
Checksum:i1ED829A655ED03F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661M → T in AAT93128. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72881 Genomic DNA. Translation: CAA97099.1.
AY693109 Genomic DNA. Translation: AAT93128.1.
BK006941 Genomic DNA. Translation: DAA08189.1.
PIRiS64401.
RefSeqiNP_011610.3. NM_001181225.3.

Genome annotation databases

EnsemblFungiiYGR096W; YGR096W; YGR096W.
GeneIDi852988.
KEGGisce:YGR096W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72881 Genomic DNA. Translation: CAA97099.1 .
AY693109 Genomic DNA. Translation: AAT93128.1 .
BK006941 Genomic DNA. Translation: DAA08189.1 .
PIRi S64401.
RefSeqi NP_011610.3. NM_001181225.3.

3D structure databases

ProteinModelPortali P53257.
SMRi P53257. Positions 12-285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33339. 15 interactions.
IntActi P53257. 1 interaction.
MINTi MINT-4487206.
STRINGi 4932.YGR096W.

Protein family/group databases

TCDBi 2.A.29.28.1. the mitochondrial carrier (mc) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR096W ; YGR096W ; YGR096W .
GeneIDi 852988.
KEGGi sce:YGR096W.

Organism-specific databases

CYGDi YGR096w.
SGDi S000003328. TPC1.

Phylogenomic databases

eggNOGi NOG310116.
GeneTreei ENSGT00550000074902.
HOGENOMi HOG000165726.
InParanoidi P53257.
KOi K15108.
OMAi YETIRIY.
OrthoDBi EOG7N0CFV.

Enzyme and pathway databases

BioCyci YEAST:G3O-30806-MONOMER.

Miscellaneous databases

NextBioi 972809.
PROi P53257.

Gene expression databases

Genevestigatori P53257.

Family and domain databases

Gene3Di 1.50.40.10. 1 hit.
InterProi IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view ]
Pfami PF00153. Mito_carr. 3 hits.
[Graphical view ]
PRINTSi PR00926. MITOCARRIER.
SUPFAMi SSF103506. SSF103506. 1 hit.
PROSITEi PS50920. SOLCAR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae."
    el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E.
    Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins."
    Belenkiy R., Haefele A., Eisen M.B., Wohlrab H.
    Biochim. Biophys. Acta 1467:207-218(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Identification and reconstitution of the yeast mitochondrial transporter for thiamine pyrophosphate."
    Marobbio C.M.T., Vozza A., Harding M., Bisaccia F., Palmieri F., Walker J.E.
    EMBO J. 21:5653-5661(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPC1_YEAST
AccessioniPrimary (citable) accession number: P53257
Secondary accession number(s): D6VUM8, Q6B1H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3