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Protein

Mitochondrial thiamine pyrophosphate carrier 1

Gene

TPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.3 Publications

Kineticsi

  1. KM=0.51 mM for dATP uptake by unloaded proteolipososmes1 Publication
  1. Vmax=38 µmol/min/g enzyme with dATP as substrate uptaken by unloaded proteolipososmes1 Publication

GO - Molecular functioni

  • thiamine transmembrane transporter activity Source: SGD

GO - Biological processi

  • thiamine pyrophosphate transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30806-MONOMER.

Protein family/group databases

TCDBi2.A.29.28.1. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial thiamine pyrophosphate carrier 1
Gene namesi
Name:TPC1
Ordered Locus Names:YGR096W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR096W.
SGDiS000003328. TPC1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 30Helical; Name=1Sequence analysisAdd BLAST17
Transmembranei84 – 100Helical; Name=2Sequence analysisAdd BLAST17
Transmembranei116 – 136Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei170 – 186Helical; Name=4Sequence analysisAdd BLAST17
Transmembranei217 – 233Helical; Name=5Sequence analysisAdd BLAST17
Transmembranei285 – 302Helical; Name=6Sequence analysisAdd BLAST18

GO - Cellular componenti

  • integral component of mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000906961 – 314Mitochondrial thiamine pyrophosphate carrier 1Add BLAST314

Proteomic databases

PRIDEiP53257.

Expressioni

Inductioni

Expression is regulated by carbon source, with very low expression with succinate, acetate, ethanol or pyruvate as carbon source.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi33339. 15 interactors.
IntActiP53257. 1 interactor.
MINTiMINT-4487206.

Structurei

3D structure databases

ProteinModelPortaliP53257.
SMRiP53257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 103Solcar 1Add BLAST90
Repeati110 – 195Solcar 2Add BLAST86
Repeati210 – 310Solcar 3Add BLAST101

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074902.
HOGENOMiHOG000165726.
InParanoidiP53257.
KOiK15108.
OMAiTAPMDTI.
OrthoDBiEOG092C3KDM.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN
60 70 80 90 100
GLKPFGSQVM EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF
110 120 130 140 150
NRYLTPFGLE ARLHSLVVGA FAGITSSIVS YPFDVLRTRL VANNQMHSMS
160 170 180 190 200
ITREVRDIWK LEGLPGFFKG SIASMTTITL TASIMFGTYE TIRIYCDENE
210 220 230 240 250
KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM QFMNSKHLEK
260 270 280 290 300
FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS
310
FWGYETAIHY LRMY
Length:314
Mass (Da):35,137
Last modified:October 1, 1996 - v1
Checksum:i1ED829A655ED03F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66M → T in AAT93128 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72881 Genomic DNA. Translation: CAA97099.1.
AY693109 Genomic DNA. Translation: AAT93128.1.
BK006941 Genomic DNA. Translation: DAA08189.1.
PIRiS64401.
RefSeqiNP_011610.3. NM_001181225.3.

Genome annotation databases

EnsemblFungiiYGR096W; YGR096W; YGR096W.
GeneIDi852988.
KEGGisce:YGR096W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72881 Genomic DNA. Translation: CAA97099.1.
AY693109 Genomic DNA. Translation: AAT93128.1.
BK006941 Genomic DNA. Translation: DAA08189.1.
PIRiS64401.
RefSeqiNP_011610.3. NM_001181225.3.

3D structure databases

ProteinModelPortaliP53257.
SMRiP53257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33339. 15 interactors.
IntActiP53257. 1 interactor.
MINTiMINT-4487206.

Protein family/group databases

TCDBi2.A.29.28.1. the mitochondrial carrier (mc) family.

Proteomic databases

PRIDEiP53257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR096W; YGR096W; YGR096W.
GeneIDi852988.
KEGGisce:YGR096W.

Organism-specific databases

EuPathDBiFungiDB:YGR096W.
SGDiS000003328. TPC1.

Phylogenomic databases

GeneTreeiENSGT00550000074902.
HOGENOMiHOG000165726.
InParanoidiP53257.
KOiK15108.
OMAiTAPMDTI.
OrthoDBiEOG092C3KDM.

Enzyme and pathway databases

BioCyciYEAST:G3O-30806-MONOMER.

Miscellaneous databases

PROiP53257.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPC1_YEAST
AccessioniPrimary (citable) accession number: P53257
Secondary accession number(s): D6VUM8, Q6B1H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.