P53257 (TPC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial thiamine pyrophosphate carrier 1

Gene names

Name:	TPC1
Ordered Locus Names:	YGR096W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria. Ref.4 Ref.5 Ref.6
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.7.
Induction	Expression is regulated by carbon source, with very low expression with succinate, acetate, ethanol or pyruvate as carbon source. Ref.6
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.
Biophysicochemical properties	Kinetic parameters: KM=0.51 mM for dATP uptake by unloaded proteolipososmes Ref.6 Vmax=38 µmol/min/g enzyme with dATP as substrate uptaken by unloaded proteolipososmes

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	thiamine pyrophosphate transport Inferred from direct assay Ref.6. Source: SGD
Cellular_component	integral to mitochondrial inner membrane Inferred from direct assay Ref.6. Source: SGD
Molecular_function	thiamine transmembrane transporter activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Mitochondrial thiamine pyrophosphate carrier 1		PRO_0000090696
Regions
	Transmembrane	14 – 30	17	Helical; Name=1; Potential
	Transmembrane	84 – 100	17	Helical; Name=2; Potential
	Transmembrane	116 – 136	21	Helical; Name=3; Potential
	Transmembrane	170 – 186	17	Helical; Name=4; Potential
	Transmembrane	217 – 233	17	Helical; Name=5; Potential
	Transmembrane	285 – 302	18	Helical; Name=6; Potential
	Repeat	14 – 103	90	Solcar 1
	Repeat	110 – 195	86	Solcar 2
	Repeat	210 – 310	101	Solcar 3
Experimental info
	Sequence conflict	66	1	M → T in AAT93128. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P53257 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1ED829A655ED03F4	FASTA	314	35,137
	10 20 30 40 50 60 MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN GLKPFGSQVM 70 80 90 100 110 120 EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF NRYLTPFGLE ARLHSLVVGA 130 140 150 160 170 180 FAGITSSIVS YPFDVLRTRL VANNQMHSMS ITREVRDIWK LEGLPGFFKG SIASMTTITL 190 200 210 220 230 240 TASIMFGTYE TIRIYCDENE KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM 250 260 270 280 290 300 QFMNSKHLEK FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS 310 FWGYETAIHY LRMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae." el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E. Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins." Belenkiy R., Haefele A., Eisen M.B., Wohlrab H. Biochim. Biophys. Acta 1467:207-218(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Identification and reconstitution of the yeast mitochondrial transporter for thiamine pyrophosphate." Marobbio C.M.T., Vozza A., Harding M., Bisaccia F., Palmieri F., Walker J.E. EMBO J. 21:5653-5661(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72881 Genomic DNA. Translation: CAA97099.1. AY693109 Genomic DNA. Translation: AAT93128.1. BK006941 Genomic DNA. Translation: DAA08189.1.
PIR	S64401.
RefSeq	NP_011610.3. NM_001181225.3.
3D structure databases
ProteinModelPortal	P53257.
SMR	P53257. Positions 12-305.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53257. 1 interaction.
MINT	MINT-4487206.
STRING	4932.YGR096W.
Protein family/group databases
TCDB	2.A.29.28.1. mitochondrial carrier (MC) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR096W; YGR096W; YGR096W.
GeneID	852988.
KEGG	sce:YGR096W. sce:YGR102C.
Organism-specific databases
CYGD	YGR096w.
SGD	S000003328. TPC1.
Phylogenomic databases
eggNOG	NOG310116.
GeneTree	ENSGT00550000074902.
HOGENOM	HOG000165726.
KO	K15108.
OMA	YETIRIY.
OrthoDB	EOG4S1XH1.
Gene expression databases
Genevestigator	P53257.
GermOnline	YGR096W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.50.40.10. 1 hit.
InterPro	IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	972809.

Entry information

Entry name

TPC1_YEAST

Accession

Primary (citable) accession number: P53257
Secondary accession number(s): D6VUM8, Q6B1H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program