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P53257

- TPC1_YEAST

UniProt

P53257 - TPC1_YEAST

Protein

Mitochondrial thiamine pyrophosphate carrier 1

Gene

TPC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria.3 Publications

    Kineticsi

    1. KM=0.51 mM for dATP uptake by unloaded proteolipososmes1 Publication

    Vmax=38 µmol/min/g enzyme with dATP as substrate uptaken by unloaded proteolipososmes1 Publication

    GO - Molecular functioni

    1. thiamine transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. thiamine pyrophosphate transport Source: SGD
    2. thiamine transmembrane transport Source: GOC

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30806-MONOMER.

    Protein family/group databases

    TCDBi2.A.29.28.1. the mitochondrial carrier (mc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial thiamine pyrophosphate carrier 1
    Gene namesi
    Name:TPC1
    Ordered Locus Names:YGR096W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR096w.
    SGDiS000003328. TPC1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of mitochondrial inner membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Mitochondrial thiamine pyrophosphate carrier 1PRO_0000090696Add
    BLAST

    Expressioni

    Inductioni

    Expression is regulated by carbon source, with very low expression with succinate, acetate, ethanol or pyruvate as carbon source.1 Publication

    Gene expression databases

    GenevestigatoriP53257.

    Interactioni

    Protein-protein interaction databases

    BioGridi33339. 15 interactions.
    IntActiP53257. 1 interaction.
    MINTiMINT-4487206.
    STRINGi4932.YGR096W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53257.
    SMRiP53257. Positions 12-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3017Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei84 – 10017Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei116 – 13621Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei170 – 18617Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei217 – 23317Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei285 – 30218Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati14 – 10390Solcar 1Add
    BLAST
    Repeati110 – 19586Solcar 2Add
    BLAST
    Repeati210 – 310101Solcar 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 Solcar repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG310116.
    GeneTreeiENSGT00750000118281.
    HOGENOMiHOG000165726.
    KOiK15108.
    OMAiYETIRIY.
    OrthoDBiEOG7N0CFV.

    Family and domain databases

    Gene3Di1.50.40.10. 1 hit.
    InterProiIPR002067. Mit_carrier.
    IPR018108. Mitochondrial_sb/sol_carrier.
    IPR023395. Mt_carrier_dom.
    [Graphical view]
    PfamiPF00153. Mito_carr. 3 hits.
    [Graphical view]
    PRINTSiPR00926. MITOCARRIER.
    SUPFAMiSSF103506. SSF103506. 1 hit.
    PROSITEiPS50920. SOLCAR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53257-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN    50
    GLKPFGSQVM EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF 100
    NRYLTPFGLE ARLHSLVVGA FAGITSSIVS YPFDVLRTRL VANNQMHSMS 150
    ITREVRDIWK LEGLPGFFKG SIASMTTITL TASIMFGTYE TIRIYCDENE 200
    KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM QFMNSKHLEK 250
    FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS 300
    FWGYETAIHY LRMY 314
    Length:314
    Mass (Da):35,137
    Last modified:October 1, 1996 - v1
    Checksum:i1ED829A655ED03F4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661M → T in AAT93128. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72881 Genomic DNA. Translation: CAA97099.1.
    AY693109 Genomic DNA. Translation: AAT93128.1.
    BK006941 Genomic DNA. Translation: DAA08189.1.
    PIRiS64401.
    RefSeqiNP_011610.3. NM_001181225.3.

    Genome annotation databases

    EnsemblFungiiYGR096W; YGR096W; YGR096W.
    GeneIDi852988.
    KEGGisce:YGR096W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72881 Genomic DNA. Translation: CAA97099.1 .
    AY693109 Genomic DNA. Translation: AAT93128.1 .
    BK006941 Genomic DNA. Translation: DAA08189.1 .
    PIRi S64401.
    RefSeqi NP_011610.3. NM_001181225.3.

    3D structure databases

    ProteinModelPortali P53257.
    SMRi P53257. Positions 12-285.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33339. 15 interactions.
    IntActi P53257. 1 interaction.
    MINTi MINT-4487206.
    STRINGi 4932.YGR096W.

    Protein family/group databases

    TCDBi 2.A.29.28.1. the mitochondrial carrier (mc) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR096W ; YGR096W ; YGR096W .
    GeneIDi 852988.
    KEGGi sce:YGR096W.

    Organism-specific databases

    CYGDi YGR096w.
    SGDi S000003328. TPC1.

    Phylogenomic databases

    eggNOGi NOG310116.
    GeneTreei ENSGT00750000118281.
    HOGENOMi HOG000165726.
    KOi K15108.
    OMAi YETIRIY.
    OrthoDBi EOG7N0CFV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30806-MONOMER.

    Miscellaneous databases

    NextBioi 972809.
    PROi P53257.

    Gene expression databases

    Genevestigatori P53257.

    Family and domain databases

    Gene3Di 1.50.40.10. 1 hit.
    InterProi IPR002067. Mit_carrier.
    IPR018108. Mitochondrial_sb/sol_carrier.
    IPR023395. Mt_carrier_dom.
    [Graphical view ]
    Pfami PF00153. Mito_carr. 3 hits.
    [Graphical view ]
    PRINTSi PR00926. MITOCARRIER.
    SUPFAMi SSF103506. SSF103506. 1 hit.
    PROSITEi PS50920. SOLCAR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae."
      el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E.
      Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins."
      Belenkiy R., Haefele A., Eisen M.B., Wohlrab H.
      Biochim. Biophys. Acta 1467:207-218(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Identification and reconstitution of the yeast mitochondrial transporter for thiamine pyrophosphate."
      Marobbio C.M.T., Vozza A., Harding M., Bisaccia F., Palmieri F., Walker J.E.
      EMBO J. 21:5653-5661(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTPC1_YEAST
    AccessioniPrimary (citable) accession number: P53257
    Secondary accession number(s): D6VUM8, Q6B1H1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3