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P53257 (TPC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial thiamine pyrophosphate carrier 1
Gene names
Name:TPC1
Ordered Locus Names:YGR096W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial transporter that mediates uptake of thiamine pyrophosphate (ThPP) into mitochondria. Ref.4 Ref.5 Ref.6

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.7.

Induction

Expression is regulated by carbon source, with very low expression with succinate, acetate, ethanol or pyruvate as carbon source. Ref.6

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Biophysicochemical properties

Kinetic parameters:

KM=0.51 mM for dATP uptake by unloaded proteolipososmes Ref.6

Vmax=38 µmol/min/g enzyme with dATP as substrate uptaken by unloaded proteolipososmes

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Mitochondrial thiamine pyrophosphate carrier 1
PRO_0000090696

Regions

Transmembrane14 – 3017Helical; Name=1; Potential
Transmembrane84 – 10017Helical; Name=2; Potential
Transmembrane116 – 13621Helical; Name=3; Potential
Transmembrane170 – 18617Helical; Name=4; Potential
Transmembrane217 – 23317Helical; Name=5; Potential
Transmembrane285 – 30218Helical; Name=6; Potential
Repeat14 – 10390Solcar 1
Repeat110 – 19586Solcar 2
Repeat210 – 310101Solcar 3

Experimental info

Sequence conflict661M → T in AAT93128. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P53257 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 1ED829A655ED03F4

FASTA31435,137
        10         20         30         40         50         60 
MFKEEDSLRK GQNVAAWKTL LAGAVSGLLA RSITAPMDTI KIRLQLTPAN GLKPFGSQVM 

        70         80         90        100        110        120 
EVARSMIKNE GIRSFWKGNI PGSLLYVTYG SAQFSSYSLF NRYLTPFGLE ARLHSLVVGA 

       130        140        150        160        170        180 
FAGITSSIVS YPFDVLRTRL VANNQMHSMS ITREVRDIWK LEGLPGFFKG SIASMTTITL 

       190        200        210        220        230        240 
TASIMFGTYE TIRIYCDENE KTTAAHKKWE LATLNHSAGT IGGVIAKIIT FPLETIRRRM 

       250        260        270        280        290        300 
QFMNSKHLEK FSRHSSVYGS YKGYGFARIG LQILKQEGVS SLYRGILVAL SKTIPTTFVS 

       310 
FWGYETAIHY LRMY 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae."
el Moualij B., Duyckaerts C., Lamotte-Brasseur J., Sluse F.E.
Yeast 13:573-581(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins."
Belenkiy R., Haefele A., Eisen M.B., Wohlrab H.
Biochim. Biophys. Acta 1467:207-218(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Identification and reconstitution of the yeast mitochondrial transporter for thiamine pyrophosphate."
Marobbio C.M.T., Vozza A., Harding M., Bisaccia F., Palmieri F., Walker J.E.
EMBO J. 21:5653-5661(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72881 Genomic DNA. Translation: CAA97099.1.
AY693109 Genomic DNA. Translation: AAT93128.1.
BK006941 Genomic DNA. Translation: DAA08189.1.
PIRS64401.
RefSeqNP_011610.3. NM_001181225.3.

3D structure databases

ProteinModelPortalP53257.
SMRP53257. Positions 12-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33339. 15 interactions.
IntActP53257. 1 interaction.
MINTMINT-4487206.
STRING4932.YGR096W.

Protein family/group databases

TCDB2.A.29.28.1. the mitochondrial carrier (mc) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR096W; YGR096W; YGR096W.
GeneID852988.
KEGGsce:YGR096W.

Organism-specific databases

CYGDYGR096w.
SGDS000003328. TPC1.

Phylogenomic databases

eggNOGNOG310116.
GeneTreeENSGT00750000118281.
HOGENOMHOG000165726.
KOK15108.
OMAYETIRIY.
OrthoDBEOG7N0CFV.

Enzyme and pathway databases

BioCycYEAST:G3O-30806-MONOMER.

Gene expression databases

GenevestigatorP53257.

Family and domain databases

Gene3D1.50.40.10. 1 hit.
InterProIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSPR00926. MITOCARRIER.
SUPFAMSSF103506. SSF103506. 1 hit.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio972809.
PROP53257.

Entry information

Entry nameTPC1_YEAST
AccessionPrimary (citable) accession number: P53257
Secondary accession number(s): D6VUM8, Q6B1H1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families