P53250 (TWF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Twinfilin-1

Alternative name(s):
Twinfilin-A

Gene names

Name:	TWF1
Ordered Locus Names:	YGR080W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. Prevents actin filament assembly by forming a 1:1 complex with actin monomers, and inhibits the nucleotide exchange reaction of actin monomers By similarity.
Subunit structure	Interacts with G-actin; ADP-actin form By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Miscellaneous	Present with 1470 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. Contains 2 ADF-H domains.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin polymerization or depolymerization Traceable author statement PubMed 10652251. Source: SGD bipolar cellular bud site selection Traceable author statement PubMed 10652251. Source: SGD sequestering of actin monomers Inferred from direct assay Ref.3. Source: SGD
Cellular_component	actin cortical patch Traceable author statement PubMed 10652251. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD
Molecular_function	actin monomer binding Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Twinfilin-1		PRO_0000214953
Regions
	Domain	5 – 132	128	ADF-H 1
	Domain	173 – 300	128	ADF-H 2
Amino acid modifications
	Modified residue	167	1	Phosphoserine Ref.5
	Modified residue	172	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53250 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D9213BBB2D953DBD	FASTA	332	37,071
	10 20 30 40 50 60 MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE ELVQLASQER 70 80 90 100 110 120 EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL ARQVGSNSLS TEQPLITDAQ 130 140 150 160 170 180 DLVDLKNFDS ARPAGQNKPL THDEEMQIEI NKQQALLRKN TSVKLVSQDS ASPLSLTFRV 190 200 210 220 230 240 NSEKPINEIL DSEGKNLIIF QIDPSNETIQ IVQSDTCPSV DELYIDLPGP SYTIFRQGDS 250 260 270 280 290 300 SFFIYSCPSG SKVKDRMIYA SNKNGFINYL KNDQKIAFSK VVEIGDFVEL DKSLLMATNK 310 320 330 EDSLDHGSNP DLPNKSNLKF NKPKGPLRKR RT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer-sequestering protein." Goode B.L., Drubin D.G., Lappalainen P. J. Cell Biol. 142:723-733(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167 AND SER-172, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72865 Genomic DNA. Translation: CAA97082.1. BK006941 Genomic DNA. Translation: DAA08173.1.
PIR	S64375.
RefSeq	NP_011594.3. NM_001181209.3.
3D structure databases
ProteinModelPortal	P53250.
SMR	P53250. Positions 28-112, 172-300.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2202N.
IntAct	P53250. 7 interactions.
MINT	MINT-557613.
STRING	4932.YGR080W.
Proteomic databases
PaxDb	P53250.
PeptideAtlas	P53250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR080W; YGR080W; YGR080W.
GeneID	852971.
KEGG	sce:YGR080W. sce:YGR086C.
Organism-specific databases
CYGD	YGR080w.
SGD	S000003312. TWF1.
Phylogenomic databases
eggNOG	NOG263290.
GeneTree	ENSGT00530000063868.
HOGENOM	HOG000248425.
OMA	AFIYSCP.
OrthoDB	EOG49PF77.
Gene expression databases
Genevestigator	P53250.
GermOnline	YGR080W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 2 hits. [Graphical view]
SMART	SM00102. ADF. 2 hits. [Graphical view]
PROSITE	PS51263. ADF_H. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	972759.

Entry information

Entry name

TWF1_YEAST

Accession

Primary (citable) accession number: P53250
Secondary accession number(s): D6VUL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program