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Protein

Twinfilin-1

Gene

TWF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. Prevents actin filament assembly by forming a 1:1 complex with actin monomers, and inhibits the nucleotide exchange reaction of actin monomers (By similarity).By similarity

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • actin filament binding Source: SGD
  • actin monomer binding Source: SGD

GO - Biological processi

  • actin cortical patch organization Source: SGD
  • actin filament depolymerization Source: SGD
  • actin filament organization Source: SGD
  • actin filament severing Source: SGD
  • positive regulation of actin filament depolymerization Source: SGD
  • sequestering of actin monomers Source: SGD

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30792-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Twinfilin-1
Alternative name(s):
Twinfilin-A
Gene namesi
Name:TWF1
Ordered Locus Names:YGR080W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR080W
SGDiS000003312 TWF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002149531 – 332Twinfilin-1Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei167PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53250
PaxDbiP53250
PRIDEiP53250

PTM databases

iPTMnetiP53250

Interactioni

Subunit structurei

Interacts with G-actin; ADP-actin form.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • actin filament binding Source: SGD
  • actin monomer binding Source: SGD

Protein-protein interaction databases

BioGridi33322, 76 interactors
DIPiDIP-2202N
IntActiP53250, 11 interactors
MINTiP53250
STRINGi4932.YGR080W

Structurei

3D structure databases

ProteinModelPortaliP53250
SMRiP53250
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 132ADF-H 1PROSITE-ProRule annotationAdd BLAST128
Domaini173 – 300ADF-H 2PROSITE-ProRule annotationAdd BLAST128

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00530000063868
HOGENOMiHOG000248425
InParanoidiP53250
OMAiYDYLQFR
OrthoDBiEOG092C2D46

Family and domain databases

Gene3Di3.40.20.10, 2 hits
InterProiView protein in InterPro
IPR002108 ADF-H
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR028458 Twinfilin
PANTHERiPTHR13759 PTHR13759, 1 hit
PfamiView protein in Pfam
PF00241 Cofilin_ADF, 2 hits
SMARTiView protein in SMART
SM00102 ADF, 2 hits
PROSITEiView protein in PROSITE
PS51263 ADF_H, 2 hits

Sequencei

Sequence statusi: Complete.

P53250-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE
60 70 80 90 100
ELVQLASQER EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL
110 120 130 140 150
ARQVGSNSLS TEQPLITDAQ DLVDLKNFDS ARPAGQNKPL THDEEMQIEI
160 170 180 190 200
NKQQALLRKN TSVKLVSQDS ASPLSLTFRV NSEKPINEIL DSEGKNLIIF
210 220 230 240 250
QIDPSNETIQ IVQSDTCPSV DELYIDLPGP SYTIFRQGDS SFFIYSCPSG
260 270 280 290 300
SKVKDRMIYA SNKNGFINYL KNDQKIAFSK VVEIGDFVEL DKSLLMATNK
310 320 330
EDSLDHGSNP DLPNKSNLKF NKPKGPLRKR RT
Length:332
Mass (Da):37,071
Last modified:October 1, 1996 - v1
Checksum:iD9213BBB2D953DBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72865 Genomic DNA Translation: CAA97082.1
BK006941 Genomic DNA Translation: DAA08173.1
PIRiS64375
RefSeqiNP_011594.3, NM_001181209.3

Genome annotation databases

EnsemblFungiiYGR080W; YGR080W; YGR080W
GeneIDi852971
KEGGisce:YGR080W

Similar proteinsi

Entry informationi

Entry nameiTWF1_YEAST
AccessioniPrimary (citable) accession number: P53250
Secondary accession number(s): D6VUL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health