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P53250

- TWF1_YEAST

UniProt

P53250 - TWF1_YEAST

Protein

Twinfilin-1

Gene

TWF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. Prevents actin filament assembly by forming a 1:1 complex with actin monomers, and inhibits the nucleotide exchange reaction of actin monomers By similarity.By similarity

    GO - Molecular functioni

    1. actin filament binding Source: SGD
    2. actin monomer binding Source: SGD

    GO - Biological processi

    1. actin cortical patch organization Source: SGD
    2. actin filament depolymerization Source: SGD
    3. actin filament organization Source: SGD
    4. actin filament severing Source: SGD
    5. sequestering of actin monomers Source: SGD

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30792-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Twinfilin-1
    Alternative name(s):
    Twinfilin-A
    Gene namesi
    Name:TWF1
    Ordered Locus Names:YGR080W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR080w.
    SGDiS000003312. TWF1.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Twinfilin-1PRO_0000214953Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei167 – 1671Phosphoserine1 Publication
    Modified residuei172 – 1721Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53250.
    PaxDbiP53250.
    PeptideAtlasiP53250.

    Expressioni

    Gene expression databases

    GenevestigatoriP53250.

    Interactioni

    Subunit structurei

    Interacts with G-actin; ADP-actin form.By similarity

    Protein-protein interaction databases

    BioGridi33322. 37 interactions.
    DIPiDIP-2202N.
    IntActiP53250. 7 interactions.
    MINTiMINT-557613.
    STRINGi4932.YGR080W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53250.
    SMRiP53250. Positions 172-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 132128ADF-H 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini173 – 300128ADF-H 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ADF-H domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG263290.
    GeneTreeiENSGT00530000063868.
    HOGENOMiHOG000248425.
    OMAiATTINEC.
    OrthoDBiEOG7NPG3K.

    Family and domain databases

    Gene3Di3.40.20.10. 2 hits.
    InterProiIPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR028458. Twinfilin.
    [Graphical view]
    PANTHERiPTHR13759. PTHR13759. 1 hit.
    PfamiPF00241. Cofilin_ADF. 2 hits.
    [Graphical view]
    SMARTiSM00102. ADF. 2 hits.
    [Graphical view]
    PROSITEiPS51263. ADF_H. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53250-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE    50
    ELVQLASQER EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL 100
    ARQVGSNSLS TEQPLITDAQ DLVDLKNFDS ARPAGQNKPL THDEEMQIEI 150
    NKQQALLRKN TSVKLVSQDS ASPLSLTFRV NSEKPINEIL DSEGKNLIIF 200
    QIDPSNETIQ IVQSDTCPSV DELYIDLPGP SYTIFRQGDS SFFIYSCPSG 250
    SKVKDRMIYA SNKNGFINYL KNDQKIAFSK VVEIGDFVEL DKSLLMATNK 300
    EDSLDHGSNP DLPNKSNLKF NKPKGPLRKR RT 332
    Length:332
    Mass (Da):37,071
    Last modified:October 1, 1996 - v1
    Checksum:iD9213BBB2D953DBD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72865 Genomic DNA. Translation: CAA97082.1.
    BK006941 Genomic DNA. Translation: DAA08173.1.
    PIRiS64375.
    RefSeqiNP_011594.3. NM_001181209.3.

    Genome annotation databases

    EnsemblFungiiYGR080W; YGR080W; YGR080W.
    GeneIDi852971.
    KEGGisce:YGR080W.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Molecular embrace - Issue 73 of August 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72865 Genomic DNA. Translation: CAA97082.1 .
    BK006941 Genomic DNA. Translation: DAA08173.1 .
    PIRi S64375.
    RefSeqi NP_011594.3. NM_001181209.3.

    3D structure databases

    ProteinModelPortali P53250.
    SMRi P53250. Positions 172-300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33322. 37 interactions.
    DIPi DIP-2202N.
    IntActi P53250. 7 interactions.
    MINTi MINT-557613.
    STRINGi 4932.YGR080W.

    Proteomic databases

    MaxQBi P53250.
    PaxDbi P53250.
    PeptideAtlasi P53250.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR080W ; YGR080W ; YGR080W .
    GeneIDi 852971.
    KEGGi sce:YGR080W.

    Organism-specific databases

    CYGDi YGR080w.
    SGDi S000003312. TWF1.

    Phylogenomic databases

    eggNOGi NOG263290.
    GeneTreei ENSGT00530000063868.
    HOGENOMi HOG000248425.
    OMAi ATTINEC.
    OrthoDBi EOG7NPG3K.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30792-MONOMER.

    Miscellaneous databases

    NextBioi 972759.

    Gene expression databases

    Genevestigatori P53250.

    Family and domain databases

    Gene3Di 3.40.20.10. 2 hits.
    InterProi IPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR028458. Twinfilin.
    [Graphical view ]
    PANTHERi PTHR13759. PTHR13759. 1 hit.
    Pfami PF00241. Cofilin_ADF. 2 hits.
    [Graphical view ]
    SMARTi SM00102. ADF. 2 hits.
    [Graphical view ]
    PROSITEi PS51263. ADF_H. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer-sequestering protein."
      Goode B.L., Drubin D.G., Lappalainen P.
      J. Cell Biol. 142:723-733(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167 AND SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTWF1_YEAST
    AccessioniPrimary (citable) accession number: P53250
    Secondary accession number(s): D6VUL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1470 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3