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Protein

Twinfilin-1

Gene

TWF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. Prevents actin filament assembly by forming a 1:1 complex with actin monomers, and inhibits the nucleotide exchange reaction of actin monomers (By similarity).By similarity

GO - Molecular functioni

  1. actin filament binding Source: SGD
  2. actin monomer binding Source: SGD

GO - Biological processi

  1. actin cortical patch organization Source: SGD
  2. actin filament depolymerization Source: SGD
  3. actin filament organization Source: SGD
  4. actin filament severing Source: SGD
  5. sequestering of actin monomers Source: SGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30792-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Twinfilin-1
Alternative name(s):
Twinfilin-A
Gene namesi
Name:TWF1
Ordered Locus Names:YGR080W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR080w.
SGDiS000003312. TWF1.

Subcellular locationi

Cytoplasmcytoskeleton By similarity

GO - Cellular componenti

  1. actin cortical patch Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Twinfilin-1PRO_0000214953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671Phosphoserine1 Publication
Modified residuei172 – 1721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53250.
PaxDbiP53250.
PeptideAtlasiP53250.

Expressioni

Gene expression databases

GenevestigatoriP53250.

Interactioni

Subunit structurei

Interacts with G-actin; ADP-actin form.By similarity

Protein-protein interaction databases

BioGridi33322. 38 interactions.
DIPiDIP-2202N.
IntActiP53250. 7 interactions.
MINTiMINT-557613.
STRINGi4932.YGR080W.

Structurei

3D structure databases

ProteinModelPortaliP53250.
SMRiP53250. Positions 172-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 132128ADF-H 1PROSITE-ProRule annotationAdd
BLAST
Domaini173 – 300128ADF-H 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ADF-H domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG263290.
GeneTreeiENSGT00530000063868.
HOGENOMiHOG000248425.
InParanoidiP53250.
OMAiKFAWTEL.
OrthoDBiEOG7NPG3K.

Family and domain databases

Gene3Di3.40.20.10. 2 hits.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view]
PANTHERiPTHR13759. PTHR13759. 1 hit.
PfamiPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTiSM00102. ADF. 2 hits.
[Graphical view]
PROSITEiPS51263. ADF_H. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53250-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTQSGIVAE QALLHSLNEN LSADGIVIII AKISPDSTSV HQTQVARSFE
60 70 80 90 100
ELVQLASQER EPLYIFYKPE GLDKYFFVSF IPDGSPVRSR MLYASTKNTL
110 120 130 140 150
ARQVGSNSLS TEQPLITDAQ DLVDLKNFDS ARPAGQNKPL THDEEMQIEI
160 170 180 190 200
NKQQALLRKN TSVKLVSQDS ASPLSLTFRV NSEKPINEIL DSEGKNLIIF
210 220 230 240 250
QIDPSNETIQ IVQSDTCPSV DELYIDLPGP SYTIFRQGDS SFFIYSCPSG
260 270 280 290 300
SKVKDRMIYA SNKNGFINYL KNDQKIAFSK VVEIGDFVEL DKSLLMATNK
310 320 330
EDSLDHGSNP DLPNKSNLKF NKPKGPLRKR RT
Length:332
Mass (Da):37,071
Last modified:October 1, 1996 - v1
Checksum:iD9213BBB2D953DBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72865 Genomic DNA. Translation: CAA97082.1.
BK006941 Genomic DNA. Translation: DAA08173.1.
PIRiS64375.
RefSeqiNP_011594.3. NM_001181209.3.

Genome annotation databases

EnsemblFungiiYGR080W; YGR080W; YGR080W.
GeneIDi852971.
KEGGisce:YGR080W.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72865 Genomic DNA. Translation: CAA97082.1.
BK006941 Genomic DNA. Translation: DAA08173.1.
PIRiS64375.
RefSeqiNP_011594.3. NM_001181209.3.

3D structure databases

ProteinModelPortaliP53250.
SMRiP53250. Positions 172-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33322. 38 interactions.
DIPiDIP-2202N.
IntActiP53250. 7 interactions.
MINTiMINT-557613.
STRINGi4932.YGR080W.

Proteomic databases

MaxQBiP53250.
PaxDbiP53250.
PeptideAtlasiP53250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR080W; YGR080W; YGR080W.
GeneIDi852971.
KEGGisce:YGR080W.

Organism-specific databases

CYGDiYGR080w.
SGDiS000003312. TWF1.

Phylogenomic databases

eggNOGiNOG263290.
GeneTreeiENSGT00530000063868.
HOGENOMiHOG000248425.
InParanoidiP53250.
OMAiKFAWTEL.
OrthoDBiEOG7NPG3K.

Enzyme and pathway databases

BioCyciYEAST:G3O-30792-MONOMER.

Miscellaneous databases

NextBioi972759.

Gene expression databases

GenevestigatoriP53250.

Family and domain databases

Gene3Di3.40.20.10. 2 hits.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view]
PANTHERiPTHR13759. PTHR13759. 1 hit.
PfamiPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTiSM00102. ADF. 2 hits.
[Graphical view]
PROSITEiPS51263. ADF_H. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Regulation of the cortical actin cytoskeleton in budding yeast by twinfilin, a ubiquitous actin monomer-sequestering protein."
    Goode B.L., Drubin D.G., Lappalainen P.
    J. Cell Biol. 142:723-733(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167 AND SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTWF1_YEAST
AccessioniPrimary (citable) accession number: P53250
Secondary accession number(s): D6VUL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.