P53244 (ART5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arrestin-related trafficking adapter 5

Gene names

Name:	ART5
Ordered Locus Names:	YGR068C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli. Ref.6
Subunit structure	Interacts with RSP5. Ref.5 Ref.6
Post-translational modification	Ubiquitinated by RSP5. Ref.5 Ref.7
Miscellaneous	Present with 1970 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the arrestin family.

Ontologies

Keywords
Biological process	Endocytosis
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ubiquitin protein ligase binding Inferred from physical interaction Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	4	EBI-23201,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Arrestin-related trafficking adapter 5		PRO_0000202804
Regions
	Compositional bias	199 – 204	6	Poly-Ser
Amino acid modifications
	Modified residue	158	1	Phosphoserine Ref.4 Ref.8
	Modified residue	203	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P53244 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C85B0D42116C041A	FASTA	586	65,477
	10 20 30 40 50 60 MFSLSSLSSS GGHSEQKERE RISYFDIRIN SPYKDIILIQ GSPLELSSIP LSGNLVISVK 70 80 90 100 110 120 NEIVVKKISL RLVGRFKLEF LQVGRYKKNS SSLASLVKEK RKIFECYWDN LLVSSKGDVL 130 140 150 160 170 180 VGGENAENQH NSSSGRSTSN QDMDTSGNAI FLSKRSLSSP VFNKIIRRKT HSSHRKILEL 190 200 210 220 230 240 PENGVTGTPF EGLRENARSR SSSSNTLNNN SHSYSNRDGS GSSYLFLMKR GNYELPFNTM 250 260 270 280 290 300 LPPEVCETIE GLQSGSILYS FEAIIDGRQL WDTDLSVHTS PHGPIGSTST SGNGMRTKNK 310 320 330 340 350 360 IIIKKFKYLR ILRTLSMDNL AMQEEISVGN TWRDKLQYET SIPSRAVPIG STTPVKIKIF 370 380 390 400 410 420 PFEKNIRLDR IEMALIQYYA MKDSSAQIYD DEIAVMKITH LADFGPLTDK LDVDCPFTIP 430 440 450 460 470 480 DNLKQITQDC CLQDNLIRVM HKLQVRILLQ RQVDGEYKNL EIKAQLPMLL FISPHLPMKG 490 500 510 520 530 540 RLVLFDKHDG KIHFRPGELV PLFLTTYPAQ GLTPGVELNS TTTAHLALPQ PPPNYHESTN 550 560 570 580 DHLMPALQPL GADSVVLTVP SYEQAQAQAS ASSYVTGSVP AYCDDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158, MASS SPECTROMETRY. Strain: YAL6B.
[5]	"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast." Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D. Mol. Syst. Biol. 3:116-116(2007) [PubMed: 17551511] [Abstract] Cited for: INTERACTION WITH RSP5, UBIQUITINATION BY RSP5.
[6]	"Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface." Lin C.H., MacGurn J.A., Chu T., Stefan C.J., Emr S.D. Cell 135:714-725(2008) [PubMed: 18976803] [Abstract] Cited for: INTERACTION WITH RPS5, FUNCTION.
[7]	"Functional dissection of a HECT ubiquitin E3 ligase." Lu J., Lin Y., Qian J., Tao S., Zhu J., Pickart C., Zhu H. Mol. Cell. Proteomics 7:35-45(2008) [PubMed: 17951556] [Abstract] Cited for: UBIQUITINATION BY RSP5.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-158 AND SER-203, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72853 Genomic DNA. Translation: CAA97070.1. BK006941 Genomic DNA. Translation: DAA08163.1.
PIR	S64363.
RefSeq	NP_011582.1. NM_001181197.1.
3D structure databases
ProteinModelPortal	P53244.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1864N.
IntAct	P53244. 12 interactions.
MINT	MINT-396449.
STRING	P53244.
Proteomic databases
PeptideAtlas	P53244.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR068C; YGR068C; YGR068C.
GeneID	852960.
KEGG	sce:YGR068C.
NMPDR	fig|4932.3.peg.2698.
Organism-specific databases
CYGD	YGR068c.
SGD	S000003300. ART5.
Phylogenomic databases
eggNOG	fuNOG09027.
GeneTree	EFGT00050000005012.
HOGENOM	HBG204359.
OMA	GKIHFRP.
OrthoDB	EOG4SR15S.
Gene expression databases
ArrayExpress	P53244.
Genevestigator	P53244.
GermOnline	YGR068C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011022. Arrestin_C-like. [Graphical view]
Pfam	PF02752. Arrestin_C. 1 hit. [Graphical view]
SMART	SM01017. Arrestin_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972729.

Entry information

Entry name

ART5_YEAST

Accession

Primary (citable) accession number: P53244
Secondary accession number(s): D6VUK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program