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P53244 (ART5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arrestin-related trafficking adapter 5
Gene names
Name:ART5
Ordered Locus Names:YGR068C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length586 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May regulate endocytosis by recruiting RSP5 ubiquitin ligase activity to specific plasma membrane proteins in response to extracellular stimuli. Ref.5

Subunit structure

Interacts with RSP5. Ref.4 Ref.5

Post-translational modification

Ubiquitinated by RSP5. Ref.4 Ref.7

Miscellaneous

Present with 1970 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the arrestin family.

Ontologies

Keywords
   Biological processEndocytosis
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent endocytosis

Inferred from sequence alignment Ref.5. Source: SGD

   Molecular_functionubiquitin protein ligase binding

Inferred from physical interaction Ref.5. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RSP5P399404EBI-23201,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 586586Arrestin-related trafficking adapter 5
PRO_0000202804

Regions

Compositional bias199 – 2046Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
P53244 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: C85B0D42116C041A

FASTA58665,477
        10         20         30         40         50         60 
MFSLSSLSSS GGHSEQKERE RISYFDIRIN SPYKDIILIQ GSPLELSSIP LSGNLVISVK 

        70         80         90        100        110        120 
NEIVVKKISL RLVGRFKLEF LQVGRYKKNS SSLASLVKEK RKIFECYWDN LLVSSKGDVL 

       130        140        150        160        170        180 
VGGENAENQH NSSSGRSTSN QDMDTSGNAI FLSKRSLSSP VFNKIIRRKT HSSHRKILEL 

       190        200        210        220        230        240 
PENGVTGTPF EGLRENARSR SSSSNTLNNN SHSYSNRDGS GSSYLFLMKR GNYELPFNTM 

       250        260        270        280        290        300 
LPPEVCETIE GLQSGSILYS FEAIIDGRQL WDTDLSVHTS PHGPIGSTST SGNGMRTKNK 

       310        320        330        340        350        360 
IIIKKFKYLR ILRTLSMDNL AMQEEISVGN TWRDKLQYET SIPSRAVPIG STTPVKIKIF 

       370        380        390        400        410        420 
PFEKNIRLDR IEMALIQYYA MKDSSAQIYD DEIAVMKITH LADFGPLTDK LDVDCPFTIP 

       430        440        450        460        470        480 
DNLKQITQDC CLQDNLIRVM HKLQVRILLQ RQVDGEYKNL EIKAQLPMLL FISPHLPMKG 

       490        500        510        520        530        540 
RLVLFDKHDG KIHFRPGELV PLFLTTYPAQ GLTPGVELNS TTTAHLALPQ PPPNYHESTN 

       550        560        570        580 
DHLMPALQPL GADSVVLTVP SYEQAQAQAS ASSYVTGSVP AYCDDD 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R., Kus B., Fladd C., Wasmuth J., Tonikian R., Sidhu S., Krogan N.J., Parkinson J., Rotin D.
Mol. Syst. Biol. 3:116-116(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RSP5, UBIQUITINATION BY RSP5.
[5]"Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface."
Lin C.H., MacGurn J.A., Chu T., Stefan C.J., Emr S.D.
Cell 135:714-725(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RPS5, FUNCTION.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Functional dissection of a HECT ubiquitin E3 ligase."
Lu J., Lin Y., Qian J., Tao S., Zhu J., Pickart C., Zhu H.
Mol. Cell. Proteomics 7:35-45(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION BY RSP5.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72853 Genomic DNA. Translation: CAA97070.1.
BK006941 Genomic DNA. Translation: DAA08163.1.
PIRS64363.
RefSeqNP_011582.1. NM_001181197.1.

3D structure databases

ProteinModelPortalP53244.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33312. 21 interactions.
DIPDIP-1864N.
IntActP53244. 13 interactions.
MINTMINT-396449.
STRING4932.YGR068C.

Proteomic databases

PaxDbP53244.
PeptideAtlasP53244.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR068C; YGR068C; YGR068C.
GeneID852960.
KEGGsce:YGR068C.

Organism-specific databases

CYGDYGR068c.
SGDS000003300. ART5.

Phylogenomic databases

eggNOGNOG316809.
GeneTreeENSGT00730000112717.
OMAGKIHFRP.
OrthoDBEOG78WM17.

Enzyme and pathway databases

BioCycYEAST:G3O-30782-MONOMER.

Gene expression databases

GenevestigatorP53244.

Family and domain databases

InterProIPR011022. Arrestin_C-like.
[Graphical view]
PfamPF02752. Arrestin_C. 1 hit.
[Graphical view]
SMARTSM01017. Arrestin_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972729.

Entry information

Entry nameART5_YEAST
AccessionPrimary (citable) accession number: P53244
Secondary accession number(s): D6VUK2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families