Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vitamin H transporter

Gene

VHT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in uptake of biotin with the concomitant entry of protons.

GO - Molecular functioni

GO - Biological processi

  • biotin transport Source: SGD
  • transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Keywords - Ligandi

Biotin

Enzyme and pathway databases

BioCyciYEAST:G3O-30779-MONOMER.

Protein family/group databases

TCDBi2.A.1.14.12. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin H transporter
Alternative name(s):
H(+)/biotin symporter
Gene namesi
Name:VHT1
Ordered Locus Names:YGR065C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR065C.
SGDiS000003297. VHT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 121121ExtracellularSequence analysisAdd
BLAST
Transmembranei122 – 14221HelicalSequence analysisAdd
BLAST
Topological domaini143 – 16624CytoplasmicSequence analysisAdd
BLAST
Transmembranei167 – 18721HelicalSequence analysisAdd
BLAST
Topological domaini188 – 1903ExtracellularSequence analysis
Transmembranei191 – 21121HelicalSequence analysisAdd
BLAST
Topological domaini212 – 22413CytoplasmicSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Topological domaini246 – 29146ExtracellularSequence analysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence analysisAdd
BLAST
Topological domaini313 – 36149CytoplasmicSequence analysisAdd
BLAST
Transmembranei362 – 38221HelicalSequence analysisAdd
BLAST
Topological domaini383 – 40826ExtracellularSequence analysisAdd
BLAST
Transmembranei409 – 42921HelicalSequence analysisAdd
BLAST
Topological domaini430 – 4323CytoplasmicSequence analysis
Transmembranei433 – 45321HelicalSequence analysisAdd
BLAST
Topological domaini454 – 4607ExtracellularSequence analysis
Transmembranei461 – 48121HelicalSequence analysisAdd
BLAST
Topological domaini482 – 49211CytoplasmicSequence analysisAdd
BLAST
Transmembranei493 – 51321HelicalSequence analysisAdd
BLAST
Topological domaini514 – 52613ExtracellularSequence analysisAdd
BLAST
Transmembranei527 – 54721HelicalSequence analysisAdd
BLAST
Topological domaini548 – 59346CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Vitamin H transporterPRO_0000121372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei32 – 321PhosphoserineCombined sources
Modified residuei33 – 331PhosphoserineCombined sources
Modified residuei43 – 431PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53241.

PTM databases

iPTMnetiP53241.

Interactioni

Protein-protein interaction databases

BioGridi33309. 19 interactions.
DIPiDIP-5519N.
IntActiP53241. 10 interactions.
MINTiMINT-533395.

Structurei

3D structure databases

ProteinModelPortaliP53241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088258.
HOGENOMiHOG000179304.
InParanoidiP53241.
OMAiDTCSWNN.
OrthoDBiEOG7QZGKW.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

P53241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISNKSWRS YFPHLRKLPE DDQYLYSDDT NSSIIAEEEL HHSVDKSSKT
60 70 80 90 100
DVTAETTAVE PHPHNLRHDL PYEVRDEAGR KWWKYFDEFE YRVNKEYKKS
110 120 130 140 150
RKWYEFLYPN HTTQTKAERR LLYKLDIIIA LYFFMLCWSK SVDLNNYTNA
160 170 180 190 200
YVSNMKEDLN MKGNDYVYTS TIANVGAIVF QLPFMYLLPR FPSHIILPVM
210 220 230 240 250
DLGWTWFTFA CYRANSLAEL RAYRFILSAF GAAYYPVSQY ILGCWYAPDE
260 270 280 290 300
INSRVCLFFC GQQLGSVTSG LLQSRIFKSL NGVHGLAGWR WMFLIDAIAI
310 320 330 340 350
SLPTAIIGFF VIPGVPSKCY SLFLTDEEIR IARARNKRNQ IKDGVDKSKL
360 370 380 390 400
APLWSRKLWK KVFCTPAFWV LVVFDTCSWN NMTAYSGSYT LWLKSNTKYS
410 420 430 440 450
IAQVNNLSVI PACLGFAYVI FCAFGADLFR CKWIFMVFAA IMNTVSCALL
460 470 480 490 500
IKWDIPSKAK WYAFFTTYFS VAASPCLWSF INDFLRFDPQ VKAITWIAIY
510 520 530 540 550
SFSQSTYAWI PTLAWPTVES PRFKTGYTVS LIFGAIYGLW TFVVLFFYKR
560 570 580 590
NEKKHALGNG IILYDSNKGE ELPEFVKKNM EERDGYYYLK RSS
Length:593
Mass (Da):69,083
Last modified:October 1, 1996 - v1
Checksum:i8B91D6247867386D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72850 Genomic DNA. Translation: CAA97067.1.
BK006941 Genomic DNA. Translation: DAA08160.1.
PIRiS64360.
RefSeqiNP_011579.1. NM_001181194.1.

Genome annotation databases

EnsemblFungiiYGR065C; YGR065C; YGR065C.
GeneIDi852956.
KEGGisce:YGR065C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72850 Genomic DNA. Translation: CAA97067.1.
BK006941 Genomic DNA. Translation: DAA08160.1.
PIRiS64360.
RefSeqiNP_011579.1. NM_001181194.1.

3D structure databases

ProteinModelPortaliP53241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33309. 19 interactions.
DIPiDIP-5519N.
IntActiP53241. 10 interactions.
MINTiMINT-533395.

Protein family/group databases

TCDBi2.A.1.14.12. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiP53241.

Proteomic databases

MaxQBiP53241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR065C; YGR065C; YGR065C.
GeneIDi852956.
KEGGisce:YGR065C.

Organism-specific databases

EuPathDBiFungiDB:YGR065C.
SGDiS000003297. VHT1.

Phylogenomic databases

GeneTreeiENSGT00620000088258.
HOGENOMiHOG000179304.
InParanoidiP53241.
OMAiDTCSWNN.
OrthoDBiEOG7QZGKW.

Enzyme and pathway databases

BioCyciYEAST:G3O-30779-MONOMER.

Miscellaneous databases

PROiP53241.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of the plasma membrane H+-biotin symporter of Saccharomyces cerevisiae by rescue of a fatty acid-auxotrophic mutant."
    Stolz J., Hoja U., Meier S., Sauer N., Schweizer E.
    J. Biol. Chem. 274:18741-18746(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-33 AND SER-43, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVHT1_YEAST
AccessioniPrimary (citable) accession number: P53241
Secondary accession number(s): D6VUJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.