Reviewed, UniProtKB/Swiss-Prot P53238 (CPNSH_YEAST)

Names and origin

Protein names

Recommended name:
    Calpain small subunit homolog protein YGR058W

Gene names

Ordered Locus Names:

YGR058W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm. Nucleus. Ref.2
Miscellaneous	Present with 1630 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Contains 3 EF-hand domains.
Caution	It is unsure if this protein binds calcium as none of the 3 EF-hand domains seem to contain a canonical calcium-binding site.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	axial cellular bud site selection Inferred from mutant phenotype. Source: SGD bipolar cellular bud site selection Inferred from mutant phenotype. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD cytoplasm Ref.2 Inferred from direct assay. Source: SGD nucleus Ref.2 Inferred from direct assay. Source: SGD
Molecular function	calcium ion binding Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-23185,EBI-23185
ACK1	Q07622	1	EBI-23185,EBI-38674
LSB1	P53281	1	EBI-23185,EBI-23329

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Calpain small subunit homolog protein YGR058W		PRO_0000073720
Regions
	Domain	157 – 192	36	EF-hand 1
	Domain	224 – 259	36	EF-hand 2
	Domain	295 – 332	38	EF-hand 3

Sequences

	Sequence		Length	Mass (Da)
	P53238-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A653D285D3DA6BD7	FASTA	335	38,390
	10 20 30 40 50 60 MCAKKLKYAA GDDFVRYATP KEAMEETRRE FEKEKQRQQQ IKVTQAQTPN TRVHSAPIPL 70 80 90 100 110 120 QTQYNKNRAE NGHHSYGSPQ SYSPRHTKTP VDPRYNVIAQ KPAGRPIPPA PTHYNNLNTS 130 140 150 160 170 180 AQRIASSPPP LIHNQAVPAQ LLKKVAPASF DSREDVRDMQ VATQLFHNHD VKGKNRLTAE 190 200 210 220 230 240 ELQNLLQNDD NSHFCISSVD ALINLFGASR FGTVNQAEFI ALYKRVKSWR KVYVDNDING 250 260 270 280 290 300 SLTISVSEFH NSLQELGYLI PFEVSEKTFD QYAEFINRNG TGKELKFDKF VEALVWLMRL 310 320 330 TKLFRKFDTN QEGIATIQYK DFIYATLYLG RFLPH

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72843 Genomic DNA. Translation: CAA97059.1.
PIR	S64352.
RefSeq	NP_011572.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1862N.
IntAct	P53238. 8 interactions.
Genome annotation databases
Ensembl	YGR058W. Saccharomyces cerevisiae. [Contig view]
GeneID	852949.
GenomeReviews	Gene locus YGR058W in contig Y13135_GR.
KEGG	sce:YGR058W.
NMPDR	fig|4932.3.peg.2688.
Organism-specific databases
CYGD	YGR058w.
SGD	S000003290. PEF1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53238.
OMA	P53238. FVEALVW.
Gene expression databases
ArrayExpress	P53238.
GermOnline	YGR058W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018249. EF_HAND_2. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PROSITE	PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972702.

Entry information

Entry name

CPNSH_YEAST

Accession

Primary (citable) accession number: P53238

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)