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Protein

Eukaryotic translation initiation factor 2A

Gene

YGR054W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. Specifically associates with both 40S subunits and 80S ribosomes.2 Publications

GO - Molecular functioni

  • ribosome binding Source: GO_Central
  • translation initiation factor activity Source: SGD
  • tRNA binding Source: GO_Central

GO - Biological processi

  • regulation of translation Source: UniProtKB-KW
  • translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30771-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Gene namesi
Ordered Locus Names:YGR054W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR054W
SGDiS000003286 YGR054W

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002028001 – 642Eukaryotic translation initiation factor 2AAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei564PhosphoserineCombined sources1
Modified residuei567PhosphoserineCombined sources1
Modified residuei572PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated, probably leading to its degradation. May explain why it has a short half-life of 17 minutes.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP53235
PaxDbiP53235
PRIDEiP53235

PTM databases

iPTMnetiP53235

Interactioni

Protein-protein interaction databases

BioGridi33299, 121 interactors
DIPiDIP-5518N
IntActiP53235, 20 interactors
MINTiP53235
STRINGi4932.YGR054W

Structurei

3D structure databases

ProteinModelPortaliP53235
SMRiP53235
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati69 – 115WD 1Add BLAST47
Repeati186 – 224WD 2Add BLAST39
Repeati289 – 331WD 3Add BLAST43
Repeati374 – 419WD 4Add BLAST46

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00730000111053
HOGENOMiHOG000193775
InParanoidiP53235
KOiK15026
OMAiWQPYTTS
OrthoDBiEOG092C2TXG

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR011387 TIF2A
IPR013979 TIF_beta_prop-like
IPR015943 WD40/YVTN_repeat-like_dom_sf
PfamiView protein in Pfam
PF08662 eIF2A, 1 hit
PIRSFiPIRSF017222 eIF2A, 1 hit

Sequencei

Sequence statusi: Complete.

P53235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQFFLKTS QDIELFQSYP TFEQSNTNSK DFPVISSVLS PCGRFLALST
60 70 80 90 100
KENVKVFTGP CLDNVLLTMK LSDVYDLHFS PAGNYLSTWE RASIQDPNHK
110 120 130 140 150
NVKVWYLNKP FKKDCVSEDI VPAYEYQAKS QSGWFLQFSK LDNYGLRLFK
160 170 180 190 200
HDLKIVKLSS ANADNFDFQS PFAVLSDDET SQHFTTYLIS PAEHPTICTF
210 220 230 240 250
TPEKGGKPAQ LIIWALSEGK ITKKIASKTF FKADSCQLKW NPLGNAILCL
260 270 280 290 300
AITDFDSSNK SYYGENTLYL LSFQGVNGTL GGNSVRVSLT TGPVHDFTWS
310 320 330 340 350
PTSRQFGVIA GYMPATISFF DLRGNVVHSL PQQAKNTMLF SPSGHYILIA
360 370 380 390 400
GFGNLQGSVE ILDRLDKFKC VSKFDATNTS VCKWSPGGEF IMTATTSPRL
410 420 430 440 450
RVDNGVKIWH VSGSLVFVKE FKELLKVDWR SPCNYKTLEN KDEAFFENHI
460 470 480 490 500
INNWEPLPDS TTSSLDPKIS NKSELQIHSS VQEYISQHPS REASSNGNGS
510 520 530 540 550
KAKAGGAYKP PHARRTGGGR IVPGVPPGAA KKTIPGLVPG MSANKDANTK
560 570 580 590 600
NRRRRANKKS SETSPDSTPA PSAPASTNAP TNNKETSPEE KKIRSLLKKL
610 620 630 640
RAIETLKERQ AVGDKLEDTQ VLKIQTEEKV LKDLEKLGWK DE
Length:642
Mass (Da):71,305
Last modified:October 1, 1996 - v1
Checksum:iF3C2E78504D83418
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti145G → A in strain: SK1. 1 Publication1
Natural varianti165N → K in strain: SK1. 1 Publication1
Natural varianti569P → S in strain: SK1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115391 Genomic DNA Translation: AAZ22467.1
Z72839 Genomic DNA Translation: CAA97054.1
Z72840 Genomic DNA Translation: CAA97056.1
BK006941 Genomic DNA Translation: DAA08150.1
PIRiS64348
RefSeqiNP_011568.3, NM_001181183.4

Genome annotation databases

EnsemblFungiiYGR054W; YGR054W; YGR054W
GeneIDi852945
KEGGisce:YGR054W

Entry informationi

Entry nameiEIF2A_YEAST
AccessioniPrimary (citable) accession number: P53235
Secondary accession number(s): D6VUI9, Q45U32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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