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Protein

Transaldolase NQM1

Gene

NQM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.

Catalytic activityi

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate.PROSITE-ProRule annotation1 Publication

Pathwayi: pentose phosphate pathway

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Transaldolase (TAL1), Transaldolase NQM1 (NQM1)
  3. no protein annotated in this organism
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei144Schiff-base intermediate with substratePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cellular response to oxidative stress Source: SGD
  • chronological cell aging Source: SGD
  • pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pentose shunt

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciYEAST:YGR043C-MONOMER.
BRENDAi2.2.1.2. 984.
ReactomeiR-SCE-163754. Insulin effects increased synthesis of Xylulose-5-Phosphate.
R-SCE-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00414.

Names & Taxonomyi

Protein namesi
Recommended name:
Transaldolase NQM1 (EC:2.2.1.2)
Alternative name(s):
Non-quiescent mutant protein 1
Gene namesi
Name:NQM1
Ordered Locus Names:YGR043C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR043C.
SGDiS000003275. NQM1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001735752 – 333Transaldolase NQM1Add BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53228.
PRIDEiP53228.

PTM databases

iPTMnetiP53228.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi33290. 26 interactors.
MINTiMINT-2491894.

Structurei

Secondary structure

1333
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 21Combined sources7
Beta strandi25 – 29Combined sources5
Helixi33 – 35Combined sources3
Helixi37 – 39Combined sources3
Beta strandi42 – 45Combined sources4
Helixi48 – 55Combined sources8
Helixi58 – 60Combined sources3
Helixi61 – 74Combined sources14
Helixi78 – 98Combined sources21
Beta strandi105 – 108Combined sources4
Helixi111 – 113Combined sources3
Helixi117 – 133Combined sources17
Helixi138 – 140Combined sources3
Beta strandi141 – 146Combined sources6
Helixi149 – 162Combined sources14
Beta strandi166 – 171Combined sources6
Helixi174 – 182Combined sources9
Beta strandi186 – 192Combined sources7
Helixi193 – 201Combined sources9
Turni209 – 211Combined sources3
Helixi213 – 228Combined sources16
Beta strandi233 – 237Combined sources5
Helixi242 – 248Combined sources7
Beta strandi251 – 257Combined sources7
Helixi258 – 266Combined sources9
Helixi277 – 280Combined sources4
Helixi281 – 283Combined sources3
Helixi294 – 303Combined sources10
Helixi305 – 333Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CQ0X-ray1.90A/B1-333[»]
ProteinModelPortaliP53228.
SMRiP53228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53228.

Family & Domainsi

Sequence similaritiesi

Belongs to the transaldolase family. Type 1 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00390000017361.
HOGENOMiHOG000281234.
InParanoidiP53228.
KOiK00616.
OMAiGRIMDFY.
OrthoDBiEOG092C3F92.

Family and domain databases

CDDicd00957. Transaldolase_TalAB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001585. TAL/FSA.
IPR004730. Transaldolase_1.
IPR018225. Transaldolase_AS.
[Graphical view]
PANTHERiPTHR10683. PTHR10683. 1 hit.
PfamiPF00923. TAL_FSA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00874. talAB. 1 hit.
PROSITEiPS01054. TRANSALDOLASE_1. 1 hit.
PS00958. TRANSALDOLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPSEKKQK VATSSLEQLK KAGTHVVADS GDFEAISKYE PQDSTTNPSL
60 70 80 90 100
ILAASKLEKY ARFIDAAVEY GRKHGKTDHE KIENAMDKIL VEFGTQILKV
110 120 130 140 150
VPGRVSTEVD ARLSFDKKAT VKKALHIIKL YKDAGVPKER VLIKIASTWE
160 170 180 190 200
GIQAARELEV KHGIHCNMTL LFSFTQAVAC AEANVTLISP FVGRIMDFYK
210 220 230 240 250
ALSGKDYTAE TDPGVLSVKK IYSYYKRHGY ATEVMAASFR NLDELKALAG
260 270 280 290 300
IDNMTLPLNL LEQLYESTDP IENKLNSESA KEEGVEKVSF INDEPHFRYV
310 320 330
LNEDQMATEK LSDGIRKFSA DIEALYKLVE EKM
Length:333
Mass (Da):37,254
Last modified:October 1, 1996 - v1
Checksum:i6492030A7DA3D343
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti290F → S in AAS56158 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72828 Genomic DNA. Translation: CAA97042.1.
AY557832 Genomic DNA. Translation: AAS56158.1.
BK006941 Genomic DNA. Translation: DAA08142.1.
PIRiS64337.
RefSeqiNP_011557.1. NM_001181172.1.

Genome annotation databases

EnsemblFungiiYGR043C; YGR043C; YGR043C.
GeneIDi852934.
KEGGisce:YGR043C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72828 Genomic DNA. Translation: CAA97042.1.
AY557832 Genomic DNA. Translation: AAS56158.1.
BK006941 Genomic DNA. Translation: DAA08142.1.
PIRiS64337.
RefSeqiNP_011557.1. NM_001181172.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CQ0X-ray1.90A/B1-333[»]
ProteinModelPortaliP53228.
SMRiP53228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33290. 26 interactors.
MINTiMINT-2491894.

PTM databases

iPTMnetiP53228.

Proteomic databases

MaxQBiP53228.
PRIDEiP53228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR043C; YGR043C; YGR043C.
GeneIDi852934.
KEGGisce:YGR043C.

Organism-specific databases

EuPathDBiFungiDB:YGR043C.
SGDiS000003275. NQM1.

Phylogenomic databases

GeneTreeiENSGT00390000017361.
HOGENOMiHOG000281234.
InParanoidiP53228.
KOiK00616.
OMAiGRIMDFY.
OrthoDBiEOG092C3F92.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00414.
BioCyciYEAST:YGR043C-MONOMER.
BRENDAi2.2.1.2. 984.
ReactomeiR-SCE-163754. Insulin effects increased synthesis of Xylulose-5-Phosphate.
R-SCE-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

EvolutionaryTraceiP53228.
PROiP53228.

Family and domain databases

CDDicd00957. Transaldolase_TalAB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001585. TAL/FSA.
IPR004730. Transaldolase_1.
IPR018225. Transaldolase_AS.
[Graphical view]
PANTHERiPTHR10683. PTHR10683. 1 hit.
PfamiPF00923. TAL_FSA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00874. talAB. 1 hit.
PROSITEiPS01054. TRANSALDOLASE_1. 1 hit.
PS00958. TRANSALDOLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAL2_YEAST
AccessioniPrimary (citable) accession number: P53228
Secondary accession number(s): D6VUI1, Q6Q5P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1920 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.