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Protein

Bud site selection protein 9

Gene

BUD9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in positioning the proximal bud pole signal.1 Publication

GO - Biological processi

  • cellular bud site selection Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-30761-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 9
Gene namesi
Name:BUD9
Ordered Locus Names:YGR041W
ORF Names:G4152
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR041W.
SGDiS000003273. BUD9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 460460ExtracellularSequence analysisAdd
BLAST
Transmembranei461 – 48121HelicalSequence analysisAdd
BLAST
Topological domaini482 – 52140CytoplasmicSequence analysisAdd
BLAST
Transmembranei522 – 54221HelicalSequence analysisAdd
BLAST
Topological domaini543 – 5475ExtracellularSequence analysis

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 547547Bud site selection protein 9PRO_0000065021Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence analysis
Modified residuei112 – 1121PhosphoserineCombined sources
Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis
Modified residuei189 – 1891PhosphoserineCombined sources
Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence analysis
Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence analysis
Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence analysis
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N- and O-glycosylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP53226.

PTM databases

iPTMnetiP53226.

Interactioni

Protein-protein interaction databases

BioGridi33288. 16 interactions.
DIPiDIP-5513N.
IntActiP53226. 3 interactions.
MINTiMINT-503624.

Structurei

3D structure databases

ProteinModelPortaliP53226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To yeast BUD8.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000066766.
InParanoidiP53226.
OMAiYESNIEA.
OrthoDBiEOG7H4F3H.

Sequencei

Sequence statusi: Complete.

P53226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKITRDVSI TTENSKSTSG SATASSASLP ENDHPIFHQP RARIRSGSLF
60 70 80 90 100
IEGSDSFPSS EVKSYNVYID DSKYSEILKG DTNSSSTDGK QVFEDARDDN
110 120 130 140 150
FHQESHRDLE DSILDLVRRD PEVAAFPLPP PNSNERNRNS SNGSSAETNL
160 170 180 190 200
NGHSSSGTIS TSVLLNMGSA EKHAGTTRGD HMESSSMKSF EKLGTRPSSL
210 220 230 240 250
FYPPPEGTAP YQGPRATVSG NKSTRQTQGT YSFPSMRYGV DLVSPVEGAV
260 270 280 290 300
DVAKSRVPNS TLNGTFPDKA FIPHEFQIPK KAWNRIPANK STSLKTPRNH
310 320 330 340 350
SLLIDILKPF EAADLANDQR SSSAVLKNTV HSNGQYNPTN ETSGTRMQDQ
360 370 380 390 400
RQKNTNEIDL EKIPNPQVPL GIAMDTMRSP NQLHEKEYES NIEAGLASGV
410 420 430 440 450
GKGDNSIKQH QYKKIPQEID RDQQLSFQME TMPIQRIDSS SIRSFDSRIY
460 470 480 490 500
GFSEIYSIPR VITTLCICLF VPPLFFFFSI NGNNGVSNYR LMRMIMNYEH
510 520 530 540
RIGLLKGFEW DIDVQWFRTL CFVLGCIEML AIFASIGIGF GVGIIRE
Length:547
Mass (Da):60,751
Last modified:October 1, 1996 - v1
Checksum:i913D622DAD52C103
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161K → T in AAG17454 (PubMed:11514631).Curated
Sequence conflicti27 – 271A → T in AAG17454 (PubMed:11514631).Curated
Sequence conflicti132 – 1321N → S in AAG17454 (PubMed:11514631).Curated
Sequence conflicti249 – 2491A → T in AAG17454 (PubMed:11514631).Curated
Sequence conflicti260 – 2601S → N in AAG17454 (PubMed:11514631).Curated
Sequence conflicti298 – 2981R → K in AAG17454 (PubMed:11514631).Curated
Sequence conflicti321 – 3211S → N in AAG17454 (PubMed:11514631).Curated
Sequence conflicti345 – 3451T → N in AAG17454 (PubMed:11514631).Curated
Sequence conflicti406 – 4061S → G in AAG17454 (PubMed:11514631).Curated
Sequence conflicti451 – 4511G → D in AAG17454 (PubMed:11514631).Curated
Sequence conflicti480 – 4801I → V in AAG17454 (PubMed:11514631).Curated
Sequence conflicti545 – 5451I → T in AAG17454 (PubMed:11514631).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302239 Genomic DNA. Translation: AAG17454.1.
Z72826 Genomic DNA. Translation: CAA97039.1.
Z72827 Genomic DNA. Translation: CAA97041.1.
BK006941 Genomic DNA. Translation: DAA08140.1.
PIRiS64332.
RefSeqiNP_011555.3. NM_001181170.3.

Genome annotation databases

EnsemblFungiiYGR041W; YGR041W; YGR041W.
GeneIDi852932.
KEGGisce:YGR041W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302239 Genomic DNA. Translation: AAG17454.1.
Z72826 Genomic DNA. Translation: CAA97039.1.
Z72827 Genomic DNA. Translation: CAA97041.1.
BK006941 Genomic DNA. Translation: DAA08140.1.
PIRiS64332.
RefSeqiNP_011555.3. NM_001181170.3.

3D structure databases

ProteinModelPortaliP53226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33288. 16 interactions.
DIPiDIP-5513N.
IntActiP53226. 3 interactions.
MINTiMINT-503624.

PTM databases

iPTMnetiP53226.

Proteomic databases

MaxQBiP53226.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR041W; YGR041W; YGR041W.
GeneIDi852932.
KEGGisce:YGR041W.

Organism-specific databases

EuPathDBiFungiDB:YGR041W.
SGDiS000003273. BUD9.

Phylogenomic databases

GeneTreeiENSGT00530000066766.
InParanoidiP53226.
OMAiYESNIEA.
OrthoDBiEOG7H4F3H.

Enzyme and pathway databases

BioCyciYEAST:G3O-30761-MONOMER.

Miscellaneous databases

PROiP53226.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bud8p and Bud9p, proteins that may mark the sites for bipolar budding in yeast."
    Harkins H.A., Page N., Schenkman L.R., De Virgilio C., Shaw S., Bussey H., Pringle J.R.
    Mol. Biol. Cell 12:2497-2518(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae."
    Zahner J.E., Harkins H.A., Pringle J.R.
    Mol. Cell. Biol. 16:1857-1870(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112 AND SER-189, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBUD9_YEAST
AccessioniPrimary (citable) accession number: P53226
Secondary accession number(s): D6VUH9, Q9HFS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.