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Protein

Dolichyldiphosphatase

Gene

CAX4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-essential protein which is required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate.2 Publications

Catalytic activityi

Dolichyl diphosphate + H2O = dolichyl phosphate + phosphate.

pH dependencei

Optimum pH is 6-7.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyldiphosphatase activity Source: SGD

GO - Biological processi

  • lipid biosynthetic process Source: SGD
  • protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YGR036C-MONOMER.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyldiphosphatase (EC:3.6.1.43)
Alternative name(s):
Dolichyl pyrophosphate phosphatase
Gene namesi
Name:CAX4
Synonyms:CWH8
Ordered Locus Names:YGR036C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR036C.
SGDiS000003268. CAX4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34LumenalSequence analysisAdd BLAST34
Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
Topological domaini56 – 131CytoplasmicSequence analysisAdd BLAST76
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Topological domaini153 – 164LumenalSequence analysisAdd BLAST12
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 239CytoplasmicSequence analysisAdd BLAST54

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002156291 – 239DolichyldiphosphataseAdd BLAST239

Proteomic databases

MaxQBiP53223.
PRIDEiP53223.

Interactioni

Protein-protein interaction databases

BioGridi33281. 44 interactors.
DIPiDIP-5237N.
IntActiP53223. 1 interactor.
MINTiMINT-563193.

Structurei

3D structure databases

ProteinModelPortaliP53223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the dolichyldiphosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000013112.
HOGENOMiHOG000201506.
InParanoidiP53223.
KOiK07252.
OMAiATTHARD.
OrthoDBiEOG092C2RUC.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequencei

Sequence statusi: Complete.

P53223-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTAAAINP NPNVIPFDDT YILYDSHDFL SFLSAYFSLM PILVLAFYLS
60 70 80 90 100
WFIITRELEA CIVAFGQLMN EIFNNVIKNI IKQPRPVSFG ASFQNDTIRS
110 120 130 140 150
GYGMPSAHSQ FMGFCFTYNS LKIYTSWKNL NFLEKCIFSG ALALLSFCVC
160 170 180 190 200
FSRVYLHYHN LDQVIVGFSV GALTGSLYFF IVGIIRELGL INWFLKLRIV
210 220 230
RLFYMTDSYN LAPLTLKENY EAYWKRINQR SFNDKSKRD
Length:239
Mass (Da):27,649
Last modified:October 1, 1996 - v1
Checksum:iC8EC49CBFC08F8F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22452.1.
Z72821 Genomic DNA. Translation: CAA97024.1.
AY557771 Genomic DNA. Translation: AAS56097.1.
BK006941 Genomic DNA. Translation: DAA08134.1.
PIRiS64327.
RefSeqiNP_011550.3. NM_001181165.3.

Genome annotation databases

EnsemblFungiiYGR036C; YGR036C; YGR036C.
GeneIDi852924.
KEGGisce:YGR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22452.1.
Z72821 Genomic DNA. Translation: CAA97024.1.
AY557771 Genomic DNA. Translation: AAS56097.1.
BK006941 Genomic DNA. Translation: DAA08134.1.
PIRiS64327.
RefSeqiNP_011550.3. NM_001181165.3.

3D structure databases

ProteinModelPortaliP53223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33281. 44 interactors.
DIPiDIP-5237N.
IntActiP53223. 1 interactor.
MINTiMINT-563193.

Proteomic databases

MaxQBiP53223.
PRIDEiP53223.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR036C; YGR036C; YGR036C.
GeneIDi852924.
KEGGisce:YGR036C.

Organism-specific databases

EuPathDBiFungiDB:YGR036C.
SGDiS000003268. CAX4.

Phylogenomic databases

GeneTreeiENSGT00390000013112.
HOGENOMiHOG000201506.
InParanoidiP53223.
KOiK07252.
OMAiATTHARD.
OrthoDBiEOG092C2RUC.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YGR036C-MONOMER.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.

Miscellaneous databases

PROiP53223.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAX4_YEAST
AccessioniPrimary (citable) accession number: P53223
Secondary accession number(s): D6VUH3, Q45U47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.