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Protein

Mitochondrial import inner membrane translocase subunit TIM21

Gene

TIM21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.2 Publications

GO - Biological processi

  1. protein import into mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30755-MONOMER.
ReactomeiREACT_334110. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM21
Gene namesi
Name:TIM21
Ordered Locus Names:YGR033C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR033c.
EuPathDBiFungiDB:YGR033C.
SGDiS000003265. TIM21.

Subcellular locationi

  1. Mitochondrion inner membrane 2 Publications; Single-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini71 – 755Mitochondrial matrixSequence Analysis
Transmembranei76 – 9621HelicalSequence AnalysisAdd
BLAST
Topological domaini97 – 239143Mitochondrial intermembraneSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: Reactome
  3. mitochondrial inner membrane presequence translocase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7070MitochondrionSequence AnalysisAdd
BLAST
Chaini71 – 239169Mitochondrial import inner membrane translocase subunit TIM21PRO_0000043166Add
BLAST

Proteomic databases

MaxQBiP53220.
PaxDbiP53220.

Expressioni

Gene expression databases

GenevestigatoriP53220.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM22Q123282EBI-23128,EBI-9132
TIM23P3289714EBI-23128,EBI-9136
TIM50Q0277610EBI-23128,EBI-30302
TOM22P493342EBI-23128,EBI-12527

Protein-protein interaction databases

BioGridi33278. 35 interactions.
DIPiDIP-3940N.
IntActiP53220. 9 interactions.
MINTiMINT-535661.
STRINGi4932.YGR033C.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi104 – 11815Combined sources
Helixi120 – 1256Combined sources
Beta strandi139 – 1424Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi151 – 1533Combined sources
Beta strandi158 – 1625Combined sources
Beta strandi168 – 17710Combined sources
Beta strandi182 – 1909Combined sources
Beta strandi199 – 2068Combined sources
Beta strandi213 – 2164Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CIUX-ray1.60A103-225[»]
2MF7NMR-A103-225[»]
SMRiP53220. Positions 103-225.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53220.

Family & Domainsi

Sequence similaritiesi

Belongs to the TIM21 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309002.
HOGENOMiHOG000093236.
InParanoidiP53220.
KOiK17796.
OMAiWVRNRPV.
OrthoDBiEOG7Z3FGD.

Family and domain databases

InterProiIPR013261. Tim21.
[Graphical view]
PANTHERiPTHR13032. PTHR13032. 1 hit.
PfamiPF08294. TIM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS
60 70 80 90 100
GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF
110 120 130 140 150
SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW
160 170 180 190 200
TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF
210 220 230
INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD
Length:239
Mass (Da):27,206
Last modified:October 1, 1996 - v1
Checksum:iFC140F3034E5B5BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391T → A in AAZ22448 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.
BK006941 Genomic DNA. Translation: DAA08130.1.
PIRiS64324.
RefSeqiNP_011547.3. NM_001181162.3.

Genome annotation databases

EnsemblFungiiYGR033C; YGR033C; YGR033C.
GeneIDi852921.
KEGGisce:YGR033C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.
BK006941 Genomic DNA. Translation: DAA08130.1.
PIRiS64324.
RefSeqiNP_011547.3. NM_001181162.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CIUX-ray1.60A103-225[»]
2MF7NMR-A103-225[»]
SMRiP53220. Positions 103-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33278. 35 interactions.
DIPiDIP-3940N.
IntActiP53220. 9 interactions.
MINTiMINT-535661.
STRINGi4932.YGR033C.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiP53220.
PaxDbiP53220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR033C; YGR033C; YGR033C.
GeneIDi852921.
KEGGisce:YGR033C.

Organism-specific databases

CYGDiYGR033c.
EuPathDBiFungiDB:YGR033C.
SGDiS000003265. TIM21.

Phylogenomic databases

eggNOGiNOG309002.
HOGENOMiHOG000093236.
InParanoidiP53220.
KOiK17796.
OMAiWVRNRPV.
OrthoDBiEOG7Z3FGD.

Enzyme and pathway databases

BioCyciYEAST:G3O-30755-MONOMER.
ReactomeiREACT_334110. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiP53220.
NextBioi972631.
PROiP53220.

Gene expression databases

GenevestigatoriP53220.

Family and domain databases

InterProiIPR013261. Tim21.
[Graphical view]
PANTHERiPTHR13032. PTHR13032. 1 hit.
PfamiPF08294. TIM21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular genetic dissection of a quantitative trait in yeast."
    Deutschbauer A.M., Davis R.W.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
    Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
    Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23 AND TOM22.
  8. "Role of Tim21 in mitochondrial translocation contact sites."
    Mokranjac D., Popov-Celeketic D., Hell K., Neupert W.
    J. Biol. Chem. 280:23437-23440(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION.

Entry informationi

Entry nameiTIM21_YEAST
AccessioniPrimary (citable) accession number: P53220
Secondary accession number(s): D6VUG9, Q45U51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.