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Protein

Mitochondrial import inner membrane translocase subunit TIM21

Gene

TIM21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30755-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM21
Gene namesi
Name:TIM21
Ordered Locus Names:YGR033C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR033C.
SGDiS000003265. TIM21.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini71 – 755Mitochondrial matrixSequence analysis
Transmembranei76 – 9621HelicalSequence analysisAdd
BLAST
Topological domaini97 – 239143Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7070MitochondrionSequence analysisAdd
BLAST
Chaini71 – 239169Mitochondrial import inner membrane translocase subunit TIM21PRO_0000043166Add
BLAST

Proteomic databases

MaxQBiP53220.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM22Q123282EBI-23128,EBI-9132
TIM23P3289714EBI-23128,EBI-9136
TIM50Q0277610EBI-23128,EBI-30302
TOM22P493342EBI-23128,EBI-12527

Protein-protein interaction databases

BioGridi33278. 36 interactions.
DIPiDIP-3940N.
IntActiP53220. 9 interactions.
MINTiMINT-535661.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi104 – 11815Combined sources
Helixi120 – 1256Combined sources
Beta strandi139 – 1424Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi151 – 1533Combined sources
Beta strandi158 – 1625Combined sources
Beta strandi168 – 17710Combined sources
Beta strandi182 – 1909Combined sources
Beta strandi199 – 2068Combined sources
Beta strandi213 – 2164Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CIUX-ray1.60A103-225[»]
2MF7NMR-A103-225[»]
ProteinModelPortaliP53220.
SMRiP53220. Positions 103-225.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53220.

Family & Domainsi

Sequence similaritiesi

Belongs to the TIM21 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000093236.
InParanoidiP53220.
KOiK17796.
OMAiVRNRPVQ.
OrthoDBiEOG092C4QRS.

Family and domain databases

InterProiIPR013261. Tim21.
[Graphical view]
PANTHERiPTHR13032. PTHR13032. 1 hit.
PfamiPF08294. TIM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS
60 70 80 90 100
GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF
110 120 130 140 150
SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW
160 170 180 190 200
TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF
210 220 230
INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD
Length:239
Mass (Da):27,206
Last modified:October 1, 1996 - v1
Checksum:iFC140F3034E5B5BB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391T → A in AAZ22448 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.
BK006941 Genomic DNA. Translation: DAA08130.1.
PIRiS64324.
RefSeqiNP_011547.3. NM_001181162.3.

Genome annotation databases

EnsemblFungiiYGR033C; YGR033C; YGR033C.
GeneIDi852921.
KEGGisce:YGR033C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.
BK006941 Genomic DNA. Translation: DAA08130.1.
PIRiS64324.
RefSeqiNP_011547.3. NM_001181162.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CIUX-ray1.60A103-225[»]
2MF7NMR-A103-225[»]
ProteinModelPortaliP53220.
SMRiP53220. Positions 103-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33278. 36 interactions.
DIPiDIP-3940N.
IntActiP53220. 9 interactions.
MINTiMINT-535661.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiP53220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR033C; YGR033C; YGR033C.
GeneIDi852921.
KEGGisce:YGR033C.

Organism-specific databases

EuPathDBiFungiDB:YGR033C.
SGDiS000003265. TIM21.

Phylogenomic databases

HOGENOMiHOG000093236.
InParanoidiP53220.
KOiK17796.
OMAiVRNRPVQ.
OrthoDBiEOG092C4QRS.

Enzyme and pathway databases

BioCyciYEAST:G3O-30755-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiP53220.
PROiP53220.

Family and domain databases

InterProiIPR013261. Tim21.
[Graphical view]
PANTHERiPTHR13032. PTHR13032. 1 hit.
PfamiPF08294. TIM21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIM21_YEAST
AccessioniPrimary (citable) accession number: P53220
Secondary accession number(s): D6VUG9, Q45U51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.