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P53220 (TIM21_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM21
Gene names
Name:TIM21
Ordered Locus Names:YGR033C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex. Ref.7 Ref.8

Subunit structure

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix. Ref.7 Ref.8

Subcellular location

Mitochondrion inner membrane; Single-pass membrane protein Ref.5 Ref.7.

Miscellaneous

Present with 3770 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TIM21 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7070Mitochondrion Potential
Chain71 – 239169Mitochondrial import inner membrane translocase subunit TIM21
PRO_0000043166

Regions

Topological domain71 – 755Mitochondrial matrix Potential
Transmembrane76 – 9621Helical; Potential
Topological domain97 – 239143Mitochondrial intermembrane Potential

Experimental info

Sequence conflict391T → A in AAZ22448. Ref.1

Secondary structure

..................... 239
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53220 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: FC140F3034E5B5BB

FASTA23927,206
        10         20         30         40         50         60 
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS GKKDDKTRNK 

        70         80         90        100        110        120 
PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF SPSGDTQLFN RAVSMVEKNK 

       130        140        150        160        170        180 
DIRSLLQCDD GITGKERLKA YGELITNDKW TRNRPIVSTK KLDKEGRTHH YMRFHVESKK 

       190        200        210        220        230 
KIALVHLEAK ESKQNYQPDF INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD 

« Hide

References

« Hide 'large scale' references
[1]"Molecular genetic dissection of a quantitative trait in yeast."
Deutschbauer A.M., Davis R.W.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SK1.
[2]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23 AND TOM22.
[8]"Role of Tim21 in mitochondrial translocation contact sites."
Mokranjac D., Popov-Celeketic D., Hell K., Neupert W.
J. Biol. Chem. 280:23437-23440(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.
BK006941 Genomic DNA. Translation: DAA08130.1.
PIRS64324.
RefSeqNP_011547.3. NM_001181162.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CIUX-ray1.60A103-225[»]
ProteinModelPortalP53220.
SMRP53220. Positions 103-225.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33278. 35 interactions.
DIPDIP-3940N.
IntActP53220. 9 interactions.
MINTMINT-535661.
STRING4932.YGR033C.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBP53220.
PaxDbP53220.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR033C; YGR033C; YGR033C.
GeneID852921.
KEGGsce:YGR033C.

Organism-specific databases

CYGDYGR033c.
SGDS000003265. TIM21.

Phylogenomic databases

eggNOGNOG309002.
HOGENOMHOG000093236.
KOK17796.
OMAVRNRPVQ.
OrthoDBEOG7Z3FGD.

Enzyme and pathway databases

BioCycYEAST:G3O-30755-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).

Gene expression databases

GenevestigatorP53220.

Family and domain databases

InterProIPR013261. Tim21.
[Graphical view]
PANTHERPTHR13032. PTHR13032. 1 hit.
PfamPF08294. TIM21. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP53220.
NextBio972631.

Entry information

Entry nameTIM21_YEAST
AccessionPrimary (citable) accession number: P53220
Secondary accession number(s): D6VUG9, Q45U51
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references