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P53220

- TIM21_YEAST

UniProt

P53220 - TIM21_YEAST

Protein

Mitochondrial import inner membrane translocase subunit TIM21

Gene

TIM21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. protein import into mitochondrial matrix Source: SGD

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30755-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM21
    Gene namesi
    Name:TIM21
    Ordered Locus Names:YGR033C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR033c.
    SGDiS000003265. TIM21.

    Subcellular locationi

    Mitochondrion inner membrane 2 Publications; Single-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Reactome
    3. mitochondrial inner membrane presequence translocase complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7070MitochondrionSequence AnalysisAdd
    BLAST
    Chaini71 – 239169Mitochondrial import inner membrane translocase subunit TIM21PRO_0000043166Add
    BLAST

    Proteomic databases

    MaxQBiP53220.
    PaxDbiP53220.

    Expressioni

    Gene expression databases

    GenevestigatoriP53220.

    Interactioni

    Subunit structurei

    Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TIM22Q123282EBI-23128,EBI-9132
    TIM23P3289714EBI-23128,EBI-9136
    TIM50Q0277610EBI-23128,EBI-30302
    TOM22P493342EBI-23128,EBI-12527

    Protein-protein interaction databases

    BioGridi33278. 35 interactions.
    DIPiDIP-3940N.
    IntActiP53220. 9 interactions.
    MINTiMINT-535661.
    STRINGi4932.YGR033C.

    Structurei

    Secondary structure

    1
    239
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi104 – 11815
    Helixi120 – 1256
    Beta strandi139 – 1424
    Beta strandi144 – 1463
    Beta strandi151 – 1533
    Beta strandi158 – 1625
    Beta strandi168 – 17710
    Beta strandi182 – 1909
    Beta strandi199 – 2068
    Beta strandi213 – 2164

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CIUX-ray1.60A103-225[»]
    ProteinModelPortaliP53220.
    SMRiP53220. Positions 103-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53220.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini71 – 755Mitochondrial matrixSequence Analysis
    Topological domaini97 – 239143Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei76 – 9621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TIM21 family.Curated

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG309002.
    HOGENOMiHOG000093236.
    KOiK17796.
    OMAiVRNRPVQ.
    OrthoDBiEOG7Z3FGD.

    Family and domain databases

    InterProiIPR013261. Tim21.
    [Graphical view]
    PANTHERiPTHR13032. PTHR13032. 1 hit.
    PfamiPF08294. TIM21. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P53220-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS    50
    GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF 100
    SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW 150
    TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF 200
    INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD 239
    Length:239
    Mass (Da):27,206
    Last modified:October 1, 1996 - v1
    Checksum:iFC140F3034E5B5BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti39 – 391T → A in AAZ22448. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115390 Genomic DNA. Translation: AAZ22448.1.
    Z72818 Genomic DNA. Translation: CAA97021.1.
    BK006941 Genomic DNA. Translation: DAA08130.1.
    PIRiS64324.
    RefSeqiNP_011547.3. NM_001181162.3.

    Genome annotation databases

    EnsemblFungiiYGR033C; YGR033C; YGR033C.
    GeneIDi852921.
    KEGGisce:YGR033C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ115390 Genomic DNA. Translation: AAZ22448.1 .
    Z72818 Genomic DNA. Translation: CAA97021.1 .
    BK006941 Genomic DNA. Translation: DAA08130.1 .
    PIRi S64324.
    RefSeqi NP_011547.3. NM_001181162.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CIU X-ray 1.60 A 103-225 [» ]
    ProteinModelPortali P53220.
    SMRi P53220. Positions 103-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33278. 35 interactions.
    DIPi DIP-3940N.
    IntActi P53220. 9 interactions.
    MINTi MINT-535661.
    STRINGi 4932.YGR033C.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P53220.
    PaxDbi P53220.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR033C ; YGR033C ; YGR033C .
    GeneIDi 852921.
    KEGGi sce:YGR033C.

    Organism-specific databases

    CYGDi YGR033c.
    SGDi S000003265. TIM21.

    Phylogenomic databases

    eggNOGi NOG309002.
    HOGENOMi HOG000093236.
    KOi K17796.
    OMAi VRNRPVQ.
    OrthoDBi EOG7Z3FGD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30755-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    EvolutionaryTracei P53220.
    NextBioi 972631.

    Gene expression databases

    Genevestigatori P53220.

    Family and domain databases

    InterProi IPR013261. Tim21.
    [Graphical view ]
    PANTHERi PTHR13032. PTHR13032. 1 hit.
    Pfami PF08294. TIM21. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular genetic dissection of a quantitative trait in yeast."
      Deutschbauer A.M., Davis R.W.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SK1.
    2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
      Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
      Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23 AND TOM22.
    8. "Role of Tim21 in mitochondrial translocation contact sites."
      Mokranjac D., Popov-Celeketic D., Hell K., Neupert W.
      J. Biol. Chem. 280:23437-23440(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION.

    Entry informationi

    Entry nameiTIM21_YEAST
    AccessioniPrimary (citable) accession number: P53220
    Secondary accession number(s): D6VUG9, Q45U51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3770 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3