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Protein

Mitochondrial import inner membrane translocase subunit TIM21

Gene

TIM21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.2 Publications

Miscellaneous

Present with 3770 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30755-MONOMER.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM21
Gene namesi
Name:TIM21
Ordered Locus Names:YGR033C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR033C.
SGDiS000003265. TIM21.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini71 – 75Mitochondrial matrixSequence analysis5
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Topological domaini97 – 239Mitochondrial intermembraneSequence analysisAdd BLAST143

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 70MitochondrionSequence analysisAdd BLAST70
ChainiPRO_000004316671 – 239Mitochondrial import inner membrane translocase subunit TIM21Add BLAST169

Proteomic databases

MaxQBiP53220.
PRIDEiP53220.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33278. 173 interactors.
DIPiDIP-3940N.
IntActiP53220. 12 interactors.
MINTiMINT-535661.
STRINGi4932.YGR033C.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi104 – 118Combined sources15
Helixi120 – 125Combined sources6
Beta strandi139 – 142Combined sources4
Beta strandi144 – 146Combined sources3
Beta strandi151 – 153Combined sources3
Beta strandi158 – 162Combined sources5
Beta strandi168 – 177Combined sources10
Beta strandi182 – 190Combined sources9
Beta strandi199 – 206Combined sources8
Beta strandi213 – 216Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CIUX-ray1.60A103-225[»]
2MF7NMR-A103-225[»]
ProteinModelPortaliP53220.
SMRiP53220.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53220.

Family & Domainsi

Sequence similaritiesi

Belongs to the TIM21 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000093236.
InParanoidiP53220.
KOiK17796.
OMAiVRNRPVQ.
OrthoDBiEOG092C4QRS.

Family and domain databases

InterProiView protein in InterPro
IPR013261. Tim21.
PANTHERiPTHR13032. PTHR13032. 1 hit.
PfamiView protein in Pfam
PF08294. TIM21. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS
60 70 80 90 100
GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF
110 120 130 140 150
SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW
160 170 180 190 200
TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF
210 220 230
INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD
Length:239
Mass (Da):27,206
Last modified:October 1, 1996 - v1
Checksum:iFC140F3034E5B5BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39T → A in AAZ22448 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.
BK006941 Genomic DNA. Translation: DAA08130.1.
PIRiS64324.
RefSeqiNP_011547.3. NM_001181162.3.

Genome annotation databases

EnsemblFungiiYGR033C; YGR033C; YGR033C.
GeneIDi852921.
KEGGisce:YGR033C.

Similar proteinsi

Entry informationi

Entry nameiTIM21_YEAST
AccessioniPrimary (citable) accession number: P53220
Secondary accession number(s): D6VUG9, Q45U51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names