Reviewed, UniProtKB/Swiss-Prot P53220 (TIM21_YEAST)

Names and origin

Protein names

Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM21

Gene names

Name:	TIM21
Ordered Locus Names:	YGR033C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex. Ref.6 Ref.7
Subunit structure	Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix. Ref.6
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein. Ref.6 Ref.4
Miscellaneous	Present with 3770 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the TIM21 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial matrix Ref.6 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane presequence translocase complex Ref.6 Ref.7 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TIM23	P32897	2	EBI-23128,EBI-9136
TOM22	P49334	1	EBI-23128,EBI-12527

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 70

70

Mitochondrion Potential

Chain

71 – 239

169

Mitochondrial import inner membrane translocase subunit TIM21

PRO_0000043166

Regions

Topological domain

71 – 75

5

Mitochondrial matrix Potential

Transmembrane

76 – 96

21

Potential

Topological domain

97 – 239

143

Mitochondrial intermembrane Potential

Experimental info

Sequence conflict

39

1

T → A in AAZ22448. Ref.1

Secondary structure

1

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239

Helix Strand Turn

Details...

Helix

104 – 118

15

Helix

120 – 125

6

Beta strand

139 – 142

4

Beta strand

144 – 146

3

Beta strand

151 – 153

3

Beta strand

158 – 162

5

Beta strand

168 – 177

10

Beta strand

182 – 190

9

Beta strand

199 – 206

8

Beta strand

213 – 216

4

Sequences

	Sequence		Length	Mass (Da)
	P53220-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FC140F3034E5B5BB	FASTA	239	27,206
	10 20 30 40 50 60 MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS GKKDDKTRNK 70 80 90 100 110 120 PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF SPSGDTQLFN RAVSMVEKNK 130 140 150 160 170 180 DIRSLLQCDD GITGKERLKA YGELITNDKW TRNRPIVSTK KLDKEGRTHH YMRFHVESKK 190 200 210 220 230 KIALVHLEAK ESKQNYQPDF INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetic dissection of a quantitative trait in yeast." Deutschbauer A.M., Davis R.W. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SK1.
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17." Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P. Cell 120:817-829(2005) [PubMed: 15797382] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH TIM23 AND TOM22.
[7]	"Role of Tim21 in mitochondrial translocation contact sites." Mokranjac D., Popov-Celeketic D., Hell K., Neupert W. J. Biol. Chem. 280:23437-23440(2005) [PubMed: 15878866] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE TIM23 COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

DQ115390 Genomic DNA. Translation: AAZ22448.1.
Z72818 Genomic DNA. Translation: CAA97021.1.

PIR

S64324.

RefSeq

NP_011547.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CIU	X-ray	1.60	A	103-225	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:3940N.

IntAct

P53220. 9 interactions.

STRING

P53220.

Protein family/group databases

TCDB

3.A.8.1.1. mitochondrial protein translocase (MPT) family.

Genome annotation databases

Ensembl

YGR033C; YGR033C; YGR033C; Saccharomyces cerevisiae. [Genome view]

GeneID

852921.

GenomeReviews

Gene locus YGR033C in contig Y13135_GR.

KEGG

sce:YGR033C.

NMPDR

fig|4932.3.peg.2660.

Organism-specific databases

CYGD

YGR033c.

SGD

S000003265. TIM21.

Phylogenomic databases

HOGENOM

P53220.

OMA

DRWTRNR.

Gene expression databases

ArrayExpress

P53220.

Genevestigator

P53220.

GermOnline

YGR033C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013261. Tim21.
[Graphical view]

Pfam

PF08294. TIM21. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

972631.

Entry information

Entry name

TIM21_YEAST

Accession

Primary (citable) accession number: P53220
Secondary accession number(s): Q45U51

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)