P53218 (POP6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleases P/MRP protein subunit POP6
EC=3.1.26.5

Alternative name(s):
RNA-processing protein POP6
RNases P/MRP 18.2 kDa subunit

Gene names

Name:	POP6
Ordered Locus Names:	YGR030C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences. Ref.5
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Subunit structure	Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex. Ref.5 Ref.8
Subcellular location	Nucleus Ref.6.
Miscellaneous	Present with 3180 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	rRNA processing tRNA processing
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intronic box C/D snoRNA processing Inferred from direct assay PubMed 18713869. Source: SGD mRNA cleavage Inferred from physical interaction PubMed 14729943. Source: SGD rRNA processing Traceable author statement Ref.5. Source: SGD tRNA processing Traceable author statement Ref.5. Source: SGD
Cellular_component	nucleolar ribonuclease P complex Inferred from direct assay Ref.5. Source: SGD ribonuclease MRP complex Inferred from direct assay Ref.5. Source: SGD
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease P activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Ribonucleases P/MRP protein subunit POP6

PRO_0000058517

Regions

Coiled coil

51 – 71

21

Potential

Secondary structure

1

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158

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Beta strand

10 – 13

4

Helix

19 – 28

10

Turn

29 – 34

6

Beta strand

38 – 48

11

Helix

55 – 66

12

Turn

71 – 74

4

Beta strand

76 – 84

9

Helix

85 – 87

3

Helix

88 – 103

16

Turn

104 – 106

3

Beta strand

110 – 120

11

Beta strand

133 – 147

15

Beta strand

155 – 157

3

Sequences

	Sequence		Length	Mass (Da)
	P53218 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6C27A73FAD521181	FASTA	158	18,210
	10 20 30 40 50 60 MINGVYYNEI SRDLDISSST QCLRFLKETV IPSLANNGNN STSIQYHGIS KNDNIKKSVN 70 80 90 100 110 120 KLDKQINMAD RSLGLQQVVC IFSYGPHIQK MLSILEIFKK GYIKNNKKIY QWNKLTSFDI 130 140 150 KREGRNELQE ERLKVPILVT LVSDSEIIDL NLHSFTKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP." Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R. Genes Dev. 12:1678-1690(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component." Salinas K., Wierzbicki S., Zhou L., Schmitt M.E. J. Biol. Chem. 280:11352-11360(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z72815 Genomic DNA. Translation: CAA97018.1.
AY558324 Genomic DNA. Translation: AAS56650.1.
BK006941 Genomic DNA. Translation: DAA08126.1.

PIR

S64321.

RefSeq

NP_011544.1. NM_001181159.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IAB	X-ray	2.70	A	1-158	[»]

ProteinModelPortal

P53218.

SMR

P53218. Positions 4-158.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6775N.

IntAct

P53218. 10 interactions.

MINT

MINT-633018.

STRING

4932.YGR030C.

Proteomic databases

PaxDb

P53218.

PeptideAtlas

P53218.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR030C; YGR030C; YGR030C.

GeneID

852918.

KEGG

sce:YGR030C.

Organism-specific databases

CYGD

YGR030c.

SGD

S000003262. POP6.

Phylogenomic databases

eggNOG

NOG69404.

HOGENOM

HOG000115608.

KO

K14524.

OMA

WNNASET.

OrthoDB

EOG4QVGNS.

Enzyme and pathway databases

BioCyc

YEAST:YGR030C-MONOMER.

Gene expression databases

ArrayExpress

P53218.

Genevestigator

P53218.

GermOnline

YGR030C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002775. DNA/RNA-bd_Alba-like.
IPR020224. RNase_P/MRP_POP6.
[Graphical view]

Pfam

PF01918. Alba. 1 hit.
[Graphical view]

ProDom

PD093696. RNase_P/MRP_su-POP6. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

P53218.

NextBio

972622.

Entry information

Entry name

POP6_YEAST

Accession

Primary (citable) accession number: P53218
Secondary accession number(s): D6VUG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program