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P53218

- POP6_YEAST

UniProt

P53218 - POP6_YEAST

Protein

Ribonucleases P/MRP protein subunit POP6

Gene

POP6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP, which cleaves pre-rRNA sequences.1 Publication

    Catalytic activityi

    Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ribonuclease P activity Source: UniProtKB-EC
    3. rRNA primary transcript binding Source: SGD

    GO - Biological processi

    1. intronic box C/D snoRNA processing Source: SGD
    2. mRNA cleavage Source: SGD
    3. RNA phosphodiester bond hydrolysis Source: GOC
    4. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    5. rRNA processing Source: SGD
    6. tRNA processing Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:YGR030C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleases P/MRP protein subunit POP6 (EC:3.1.26.5)
    Alternative name(s):
    RNA-processing protein POP6
    RNases P/MRP 18.2 kDa subunit
    Gene namesi
    Name:POP6
    Ordered Locus Names:YGR030C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR030c.
    SGDiS000003262. POP6.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. nucleolar ribonuclease P complex Source: SGD
    3. nucleus Source: SGD
    4. ribonuclease MRP complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Ribonucleases P/MRP protein subunit POP6PRO_0000058517Add
    BLAST

    Proteomic databases

    MaxQBiP53218.
    PaxDbiP53218.
    PeptideAtlasiP53218.

    Expressioni

    Gene expression databases

    GenevestigatoriP53218.

    Interactioni

    Subunit structurei

    Component of nuclear RNase P and RNase MRP complexes. RNase P consists of an RNA moiety and at least 9 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RPP1 and RPR2. RNase MRP complex consists of an RNA moiety and at least 10 protein subunits including POP1, POP3, POP4, POP5, POP6, POP7, POP8, RMP1, RPP1 and SNM1, many of which are shared with the RNase P complex.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    POP7P382913EBI-13662,EBI-13670

    Protein-protein interaction databases

    BioGridi33275. 17 interactions.
    DIPiDIP-6775N.
    IntActiP53218. 10 interactions.
    MINTiMINT-633018.
    STRINGi4932.YGR030C.

    Structurei

    Secondary structure

    1
    158
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 73
    Beta strandi10 – 134
    Helixi19 – 2810
    Turni29 – 346
    Beta strandi38 – 4811
    Helixi55 – 6612
    Turni71 – 744
    Beta strandi76 – 849
    Helixi85 – 873
    Helixi88 – 10316
    Turni104 – 1063
    Beta strandi110 – 12011
    Beta strandi133 – 14715
    Beta strandi155 – 1573

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IABX-ray2.70A1-158[»]
    ProteinModelPortaliP53218.
    SMRiP53218. Positions 4-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53218.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili51 – 7121Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG69404.
    HOGENOMiHOG000115608.
    KOiK14524.
    OMAiKENENCK.
    OrthoDBiEOG70CRK7.

    Family and domain databases

    InterProiIPR002775. DNA/RNA-bd_Alba-like.
    IPR020224. RNase_P/MRP_POP6.
    [Graphical view]
    PfamiPF01918. Alba. 1 hit.
    [Graphical view]
    ProDomiPD093696. RNase_P/MRP_su-POP6. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P53218-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINGVYYNEI SRDLDISSST QCLRFLKETV IPSLANNGNN STSIQYHGIS    50
    KNDNIKKSVN KLDKQINMAD RSLGLQQVVC IFSYGPHIQK MLSILEIFKK 100
    GYIKNNKKIY QWNKLTSFDI KREGRNELQE ERLKVPILVT LVSDSEIIDL 150
    NLHSFTKQ 158
    Length:158
    Mass (Da):18,210
    Last modified:October 1, 1996 - v1
    Checksum:i6C27A73FAD521181
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72815 Genomic DNA. Translation: CAA97018.1.
    AY558324 Genomic DNA. Translation: AAS56650.1.
    BK006941 Genomic DNA. Translation: DAA08126.1.
    PIRiS64321.
    RefSeqiNP_011544.1. NM_001181159.1.

    Genome annotation databases

    EnsemblFungiiYGR030C; YGR030C; YGR030C.
    GeneIDi852918.
    KEGGisce:YGR030C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72815 Genomic DNA. Translation: CAA97018.1 .
    AY558324 Genomic DNA. Translation: AAS56650.1 .
    BK006941 Genomic DNA. Translation: DAA08126.1 .
    PIRi S64321.
    RefSeqi NP_011544.1. NM_001181159.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IAB X-ray 2.70 A 1-158 [» ]
    ProteinModelPortali P53218.
    SMRi P53218. Positions 4-158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33275. 17 interactions.
    DIPi DIP-6775N.
    IntActi P53218. 10 interactions.
    MINTi MINT-633018.
    STRINGi 4932.YGR030C.

    Proteomic databases

    MaxQBi P53218.
    PaxDbi P53218.
    PeptideAtlasi P53218.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR030C ; YGR030C ; YGR030C .
    GeneIDi 852918.
    KEGGi sce:YGR030C.

    Organism-specific databases

    CYGDi YGR030c.
    SGDi S000003262. POP6.

    Phylogenomic databases

    eggNOGi NOG69404.
    HOGENOMi HOG000115608.
    KOi K14524.
    OMAi KENENCK.
    OrthoDBi EOG70CRK7.

    Enzyme and pathway databases

    BioCyci YEAST:YGR030C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P53218.
    NextBioi 972622.

    Gene expression databases

    Genevestigatori P53218.

    Family and domain databases

    InterProi IPR002775. DNA/RNA-bd_Alba-like.
    IPR020224. RNase_P/MRP_POP6.
    [Graphical view ]
    Pfami PF01918. Alba. 1 hit.
    [Graphical view ]
    ProDomi PD093696. RNase_P/MRP_su-POP6. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP."
      Chamberlain J.R., Lee Y., Lane W.S., Engelke D.R.
      Genes Dev. 12:1678-1690(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNASE P COMPLEX BY MASS SPECTROMETRY.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component."
      Salinas K., Wierzbicki S., Zhou L., Schmitt M.E.
      J. Biol. Chem. 280:11352-11360(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RNASE MRP COMPLEX BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPOP6_YEAST
    AccessioniPrimary (citable) accession number: P53218
    Secondary accession number(s): D6VUG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3180 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3