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Protein

tRNA(His) guanylyltransferase

Gene

THG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.1 Publication

Miscellaneous

Present with 1170 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Magnesium 1; catalyticBy similarity1
Metal bindingi29Magnesium 2; catalyticBy similarity1
Metal bindingi30Magnesium 1; via carbonyl oxygen; catalyticBy similarity1
Metal bindingi77Magnesium 1; catalyticBy similarity1
Metal bindingi77Magnesium 2; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 34GTPBy similarity6
Nucleotide bindingi76 – 77GTPBy similarity2

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • magnesium ion binding Source: InterPro
  • tRNA guanylyltransferase activity Source: SGD

GO - Biological processi

  • tRNA modification Source: SGD
  • tRNA processing Source: UniProtKB

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30749-MONOMER.
YEAST:G3O-30749-MONOMER.
BRENDAi2.7.7.79. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
tRNA-histidine guanylyltransferase
Gene namesi
Name:THG1
Ordered Locus Names:YGR024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR024C.
SGDiS000003256. THG1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002027882 – 237tRNA(His) guanylyltransferaseAdd BLAST236

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53215.
PRIDEiP53215.

PTM databases

iPTMnetiP53215.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-23112,EBI-23112

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi33266. 77 interactors.
DIPiDIP-1670N.
IntActiP53215. 73 interactors.
MINTiMINT-394323.
STRINGi4932.YGR024C.

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6
Beta strandi23 – 30Combined sources8
Helixi33 – 39Combined sources7
Helixi48 – 64Combined sources17
Turni65 – 68Combined sources4
Beta strandi69 – 74Combined sources6
Beta strandi76 – 83Combined sources8
Helixi89 – 92Combined sources4
Helixi94 – 116Combined sources23
Beta strandi118 – 120Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi130 – 139Combined sources10
Helixi140 – 171Combined sources32
Helixi175 – 182Combined sources8
Helixi187 – 195Combined sources9
Helixi207 – 210Combined sources4
Beta strandi213 – 221Combined sources9
Helixi229 – 232Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XOXX-ray3.00A/B/C/D/E/F1-237[»]
ProteinModelPortaliP53215.
SMRiP53215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
InParanoidiP53215.
KOiK10761.
OMAiNTAFWKL.
OrthoDBiEOG092C4SM4.

Family and domain databases

InterProiView protein in InterPro
IPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiView protein in Pfam
PF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSKFGYVR QFETHDVILP QCYIVVRIDG KKFHEFSKFY EFAKPNDENA
60 70 80 90 100
LKLMNACAKN LVLKYKNDII LAFGESDEYS FILKSSTTLF NRRKDKLATL
110 120 130 140 150
FGSFFTSNYV ALWAKFFPEK PLNIKHLPYF DSRCVAYPNL QTIKDYLSWR
160 170 180 190 200
YVDTHINNLY NTTFWQLIIK CGLTPQESEK KLCGTFSNEK QEILFSECGI
210 220 230
NYNNEPEMFK KGSLVTRKGE ILHINVIAQI DELFEGY
Length:237
Mass (Da):27,757
Last modified:October 1, 1996 - v1
Checksum:iCCB018E24B9EEA26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115389 Genomic DNA. Translation: AAZ22487.1.
Z72809 Genomic DNA. Translation: CAA97007.1.
AY558269 Genomic DNA. Translation: AAS56595.1.
BK006941 Genomic DNA. Translation: DAA08119.1.
PIRiS64315.
RefSeqiNP_011538.3. NM_001181153.3.

Genome annotation databases

EnsemblFungiiYGR024C; YGR024C; YGR024C.
GeneIDi852908.
KEGGisce:YGR024C.

Similar proteinsi

Entry informationi

Entry nameiTHG1_YEAST
AccessioniPrimary (citable) accession number: P53215
Secondary accession number(s): D6VUF8, Q45U59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names