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Protein

tRNA(His) guanylyltransferase

Gene

THG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.1 Publication

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Magnesium 1; catalyticBy similarity
Metal bindingi29 – 291Magnesium 2; catalyticBy similarity
Metal bindingi30 – 301Magnesium 1; via carbonyl oxygen; catalyticBy similarity
Metal bindingi77 – 771Magnesium 1; catalyticBy similarity
Metal bindingi77 – 771Magnesium 2; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346GTPBy similarity
Nucleotide bindingi76 – 772GTPBy similarity

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • magnesium ion binding Source: InterPro
  • tRNA guanylyltransferase activity Source: SGD

GO - Biological processi

  • tRNA modification Source: SGD
  • tRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16701.
YEAST:G3O-30749-MONOMER.
BRENDAi2.7.7.79. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
tRNA-histidine guanylyltransferase
Gene namesi
Name:THG1
Ordered Locus Names:YGR024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR024C.
SGDiS000003256. THG1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 237236tRNA(His) guanylyltransferasePRO_0000202788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53215.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-23112,EBI-23112

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi33266. 53 interactions.
DIPiDIP-1670N.
IntActiP53215. 74 interactions.
MINTiMINT-394323.

Structurei

3D structure databases

ProteinModelPortaliP53215.
SMRiP53215. Positions 1-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
InParanoidiP53215.
KOiK10761.
OMAiRFEYVKS.
OrthoDBiEOG092C4SM4.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSKFGYVR QFETHDVILP QCYIVVRIDG KKFHEFSKFY EFAKPNDENA
60 70 80 90 100
LKLMNACAKN LVLKYKNDII LAFGESDEYS FILKSSTTLF NRRKDKLATL
110 120 130 140 150
FGSFFTSNYV ALWAKFFPEK PLNIKHLPYF DSRCVAYPNL QTIKDYLSWR
160 170 180 190 200
YVDTHINNLY NTTFWQLIIK CGLTPQESEK KLCGTFSNEK QEILFSECGI
210 220 230
NYNNEPEMFK KGSLVTRKGE ILHINVIAQI DELFEGY
Length:237
Mass (Da):27,757
Last modified:October 1, 1996 - v1
Checksum:iCCB018E24B9EEA26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115389 Genomic DNA. Translation: AAZ22487.1.
Z72809 Genomic DNA. Translation: CAA97007.1.
AY558269 Genomic DNA. Translation: AAS56595.1.
BK006941 Genomic DNA. Translation: DAA08119.1.
PIRiS64315.
RefSeqiNP_011538.3. NM_001181153.3.

Genome annotation databases

EnsemblFungiiYGR024C; YGR024C; YGR024C.
GeneIDi852908.
KEGGisce:YGR024C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ115389 Genomic DNA. Translation: AAZ22487.1.
Z72809 Genomic DNA. Translation: CAA97007.1.
AY558269 Genomic DNA. Translation: AAS56595.1.
BK006941 Genomic DNA. Translation: DAA08119.1.
PIRiS64315.
RefSeqiNP_011538.3. NM_001181153.3.

3D structure databases

ProteinModelPortaliP53215.
SMRiP53215. Positions 1-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33266. 53 interactions.
DIPiDIP-1670N.
IntActiP53215. 74 interactions.
MINTiMINT-394323.

Proteomic databases

MaxQBiP53215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR024C; YGR024C; YGR024C.
GeneIDi852908.
KEGGisce:YGR024C.

Organism-specific databases

EuPathDBiFungiDB:YGR024C.
SGDiS000003256. THG1.

Phylogenomic databases

GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
InParanoidiP53215.
KOiK10761.
OMAiRFEYVKS.
OrthoDBiEOG092C4SM4.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16701.
YEAST:G3O-30749-MONOMER.
BRENDAi2.7.7.79. 984.

Miscellaneous databases

PROiP53215.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHG1_YEAST
AccessioniPrimary (citable) accession number: P53215
Secondary accession number(s): D6VUF8, Q45U59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1170 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.