P53207 (SNU71_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U1 small nuclear ribonucleoprotein component SNU71

Gene names

Name:	SNU71
Ordered Locus Names:	YGR013W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway. Ref.6
Subunit structure	Component of the 18S U1 snRNP particle, a subcomplex of the spliceosome. Ref.4 Ref.5 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.8.
Miscellaneous	Present with 782 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SNU71 family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Domain	Coiled coil
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from physical interaction Ref.4. Source: SGD
Cellular_component	U1 snRNP Inferred from direct assay Ref.4. Source: SGD U2-type prespliceosome Inferred from direct assay PubMed 16618970. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	5	EBI-736,EBI-701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	U1 small nuclear ribonucleoprotein component SNU71		PRO_0000202781
Regions
	Coiled coil	296 – 385	90	Potential
Amino acid modifications
	Modified residue	65	1	Phosphoserine Ref.11
	Modified residue	512	1	Phosphoserine Ref.10
	Modified residue	514	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P53207 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2659AAA62C4993D5	FASTA	620	71,383
	10 20 30 40 50 60 MRDIVFVSPQ LYLSSQEGWK SDSAKSGFIP ILKNDLQRFQ DSLKHIVDAR NSLSETLLNS 70 80 90 100 110 120 NDDGSIHNSD QNTGLNKDKE ASIADNNSAN KCATSSSRYQ ELKQFLPISL DQQIHTVSLQ 130 140 150 160 170 180 GVSSSFSRGQ IESLLDHCLN LALTETQSNS ALKVEAWSSF SSFLDTQDIF IRFSKVDEDE 190 200 210 220 230 240 AFVNTLNYCK ALFAFIRKLH EDFKIELHLD LNTKEYVEDR TGTIPSVKPE KASEFYSVFK 250 260 270 280 290 300 NIEDQTDERN SKKEQLDDSS TQYKVDTNTL SDLPSDALDQ LCKDIIEFRT KVVSIEKEKK 310 320 330 340 350 360 MKSTYEESRR QRHQMQKVFD QIRKNHSGAK GSANTEEEDT NMEDEDEEDD TEDDLALEKR 370 380 390 400 410 420 KEERDLEESN RRYEDMLHQL HSNTEPKIKS IRADIMSAEN YEEHLEKNRS LYLKELLHLA 430 440 450 460 470 480 NDVHYDHHRS FKEQEERRDE EDRAKNGNAK ELAPIQLSDG KAISAGKAAA ITLPEGTVKS 490 500 510 520 530 540 ENYNADKNVS ESSEHVKIKF DFKKAIDHSV ESSSEDEGYR ESELPPTKPS ERSAAEDRLP 550 560 570 580 590 600 FTADELNIRL TNLKESRYVD ELVREFLGVY EDELVEYILE NIRVNQSKQA LLNELRETFD 610 620 EDGETIADRL WSRKEFRLGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins." Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P. RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-38; 104-128; 191-197; 232-240; 451-461; 468-479; 505-549 AND 597-613, IDENTIFICATION IN U1 SNRNP COMPLEX.
[5]	"A generic protein purification method for protein complex characterization and proteome exploration." Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., Seraphin B. Nat. Biotechnol. 17:1030-1032(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U1 SNRNP COMPLEX, MASS SPECTROMETRY.
[6]	"A role for the Psi-U mismatch in the recognition of the 5' splice site of yeast introns by the U1 small nuclear ribonucleoprotein particle." Libri D., Duconge F., Levy L., Vinauger M. J. Biol. Chem. 277:18173-18181(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE U1 SNRNP COMPLEX.
[7]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512 AND SER-514, MASS SPECTROMETRY. Strain: ADR376.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72798 Genomic DNA. Translation: CAA96996.1. BK006941 Genomic DNA. Translation: DAA08110.1.
PIR	S64304.
RefSeq	NP_011527.1. NM_001181142.1.
3D structure databases
ProteinModelPortal	P53207.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5507N.
IntAct	P53207. 24 interactions.
MINT	MINT-480691.
STRING	4932.YGR013W.
Proteomic databases
PaxDb	P53207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR013W; YGR013W; YGR013W.
GeneID	852896.
KEGG	sce:YGR013W.
Organism-specific databases
CYGD	YGR013w.
SGD	S000003245. SNU71.
Phylogenomic databases
eggNOG	NOG311014.
HOGENOM	HOG000154360.
OMA	FENITIN.
OrthoDB	EOG49GPS0.
Gene expression databases
Genevestigator	P53207.
GermOnline	YGR013W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.20.1390.10. 1 hit.
InterPro	IPR002483. PWI_dom. [Graphical view]
SMART	SM00311. PWI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972568.

Entry information

Entry name

SNU71_YEAST

Accession

Primary (citable) accession number: P53207
Secondary accession number(s): D6VUE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program