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P53207 (SNU71_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U1 small nuclear ribonucleoprotein component SNU71
Gene names
Name:SNU71
Ordered Locus Names:YGR013W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length620 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway. Ref.6

Subunit structure

Component of the 18S U1 snRNP particle, a subcomplex of the spliceosome. Ref.4 Ref.5 Ref.7

Subcellular location

Cytoplasm. Nucleus Ref.8.

Miscellaneous

Present with 782 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SNU71 family.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentCytoplasm
Nucleus
Spliceosome
   DomainCoiled coil
   LigandRNA-binding
   Molecular functionRibonucleoprotein
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmRNA splicing, via spliceosome

Inferred from physical interaction Ref.4. Source: SGD

   Cellular_componentU1 snRNP

Inferred from direct assay Ref.4. Source: SGD

U2-type prespliceosome

Inferred from direct assay PubMed 16618970. Source: SGD

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from direct assay Ref.4. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332035EBI-736,EBI-701

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 620620U1 small nuclear ribonucleoprotein component SNU71
PRO_0000202781

Regions

Coiled coil296 – 38590 Potential

Amino acid modifications

Modified residue5121Phosphoserine Ref.10
Modified residue5141Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P53207 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2659AAA62C4993D5

FASTA62071,383
        10         20         30         40         50         60 
MRDIVFVSPQ LYLSSQEGWK SDSAKSGFIP ILKNDLQRFQ DSLKHIVDAR NSLSETLLNS 

        70         80         90        100        110        120 
NDDGSIHNSD QNTGLNKDKE ASIADNNSAN KCATSSSRYQ ELKQFLPISL DQQIHTVSLQ 

       130        140        150        160        170        180 
GVSSSFSRGQ IESLLDHCLN LALTETQSNS ALKVEAWSSF SSFLDTQDIF IRFSKVDEDE 

       190        200        210        220        230        240 
AFVNTLNYCK ALFAFIRKLH EDFKIELHLD LNTKEYVEDR TGTIPSVKPE KASEFYSVFK 

       250        260        270        280        290        300 
NIEDQTDERN SKKEQLDDSS TQYKVDTNTL SDLPSDALDQ LCKDIIEFRT KVVSIEKEKK 

       310        320        330        340        350        360 
MKSTYEESRR QRHQMQKVFD QIRKNHSGAK GSANTEEEDT NMEDEDEEDD TEDDLALEKR 

       370        380        390        400        410        420 
KEERDLEESN RRYEDMLHQL HSNTEPKIKS IRADIMSAEN YEEHLEKNRS LYLKELLHLA 

       430        440        450        460        470        480 
NDVHYDHHRS FKEQEERRDE EDRAKNGNAK ELAPIQLSDG KAISAGKAAA ITLPEGTVKS 

       490        500        510        520        530        540 
ENYNADKNVS ESSEHVKIKF DFKKAIDHSV ESSSEDEGYR ESELPPTKPS ERSAAEDRLP 

       550        560        570        580        590        600 
FTADELNIRL TNLKESRYVD ELVREFLGVY EDELVEYILE NIRVNQSKQA LLNELRETFD 

       610        620 
EDGETIADRL WSRKEFRLGT 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins."
Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.
RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-38; 104-128; 191-197; 232-240; 451-461; 468-479; 505-549 AND 597-613, IDENTIFICATION IN U1 SNRNP COMPLEX.
[5]"A generic protein purification method for protein complex characterization and proteome exploration."
Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., Seraphin B.
Nat. Biotechnol. 17:1030-1032(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"A role for the Psi-U mismatch in the recognition of the 5' splice site of yeast introns by the U1 small nuclear ribonucleoprotein particle."
Libri D., Duconge F., Levy L., Vinauger M.
J. Biol. Chem. 277:18173-18181(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE U1 SNRNP COMPLEX.
[7]"Composition and functional characterization of the yeast spliceosomal penta-snRNP."
Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512 AND SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z72798 Genomic DNA. Translation: CAA96996.1.
BK006941 Genomic DNA. Translation: DAA08110.1.
PIRS64304.
RefSeqNP_011527.1. NM_001181142.1.

3D structure databases

ProteinModelPortalP53207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33256. 51 interactions.
DIPDIP-5507N.
IntActP53207. 24 interactions.
MINTMINT-480691.
STRING4932.YGR013W.

Proteomic databases

PaxDbP53207.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR013W; YGR013W; YGR013W.
GeneID852896.
KEGGsce:YGR013W.

Organism-specific databases

CYGDYGR013w.
SGDS000003245. SNU71.

Phylogenomic databases

eggNOGNOG311014.
HOGENOMHOG000154360.
OMAANDTIDQ.
OrthoDBEOG7V76G7.

Enzyme and pathway databases

BioCycYEAST:G3O-30740-MONOMER.

Gene expression databases

GenevestigatorP53207.

Family and domain databases

Gene3D1.20.1390.10. 1 hit.
InterProIPR002483. PWI_dom.
[Graphical view]
SMARTSM00311. PWI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972568.

Entry information

Entry nameSNU71_YEAST
AccessionPrimary (citable) accession number: P53207
Secondary accession number(s): D6VUE9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families