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P53207

- SNU71_YEAST

UniProt

P53207 - SNU71_YEAST

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Protein
U1 small nuclear ribonucleoprotein component SNU71
Gene
SNU71, YGR013W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway.1 Publication

GO - Molecular functioni

  1. RNA binding Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30740-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein component SNU71
Gene namesi
Name:SNU71
Ordered Locus Names:YGR013W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR013w.
SGDiS000003245. SNU71.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. U1 snRNP Source: SGD
  2. U2-type prespliceosome Source: SGD
  3. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 620620U1 small nuclear ribonucleoprotein component SNU71
PRO_0000202781Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei512 – 5121Phosphoserine1 Publication
Modified residuei514 – 5141Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53207.
PaxDbiP53207.

Expressioni

Gene expression databases

GenevestigatoriP53207.

Interactioni

Subunit structurei

Component of the 18S U1 snRNP particle, a subcomplex of the spliceosome.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332035EBI-736,EBI-701

Protein-protein interaction databases

BioGridi33256. 51 interactions.
DIPiDIP-5507N.
IntActiP53207. 24 interactions.
MINTiMINT-480691.
STRINGi4932.YGR013W.

Structurei

3D structure databases

ProteinModelPortaliP53207.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili296 – 38590 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the SNU71 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG311014.
HOGENOMiHOG000154360.
OMAiANDTIDQ.
OrthoDBiEOG7V76G7.

Family and domain databases

Gene3Di1.20.1390.10. 1 hit.
InterProiIPR002483. PWI_dom.
[Graphical view]
SMARTiSM00311. PWI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53207-1 [UniParc]FASTAAdd to Basket

« Hide

MRDIVFVSPQ LYLSSQEGWK SDSAKSGFIP ILKNDLQRFQ DSLKHIVDAR    50
NSLSETLLNS NDDGSIHNSD QNTGLNKDKE ASIADNNSAN KCATSSSRYQ 100
ELKQFLPISL DQQIHTVSLQ GVSSSFSRGQ IESLLDHCLN LALTETQSNS 150
ALKVEAWSSF SSFLDTQDIF IRFSKVDEDE AFVNTLNYCK ALFAFIRKLH 200
EDFKIELHLD LNTKEYVEDR TGTIPSVKPE KASEFYSVFK NIEDQTDERN 250
SKKEQLDDSS TQYKVDTNTL SDLPSDALDQ LCKDIIEFRT KVVSIEKEKK 300
MKSTYEESRR QRHQMQKVFD QIRKNHSGAK GSANTEEEDT NMEDEDEEDD 350
TEDDLALEKR KEERDLEESN RRYEDMLHQL HSNTEPKIKS IRADIMSAEN 400
YEEHLEKNRS LYLKELLHLA NDVHYDHHRS FKEQEERRDE EDRAKNGNAK 450
ELAPIQLSDG KAISAGKAAA ITLPEGTVKS ENYNADKNVS ESSEHVKIKF 500
DFKKAIDHSV ESSSEDEGYR ESELPPTKPS ERSAAEDRLP FTADELNIRL 550
TNLKESRYVD ELVREFLGVY EDELVEYILE NIRVNQSKQA LLNELRETFD 600
EDGETIADRL WSRKEFRLGT 620
Length:620
Mass (Da):71,383
Last modified:October 1, 1996 - v1
Checksum:i2659AAA62C4993D5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72798 Genomic DNA. Translation: CAA96996.1.
BK006941 Genomic DNA. Translation: DAA08110.1.
PIRiS64304.
RefSeqiNP_011527.1. NM_001181142.1.

Genome annotation databases

EnsemblFungiiYGR013W; YGR013W; YGR013W.
GeneIDi852896.
KEGGisce:YGR013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72798 Genomic DNA. Translation: CAA96996.1 .
BK006941 Genomic DNA. Translation: DAA08110.1 .
PIRi S64304.
RefSeqi NP_011527.1. NM_001181142.1.

3D structure databases

ProteinModelPortali P53207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33256. 51 interactions.
DIPi DIP-5507N.
IntActi P53207. 24 interactions.
MINTi MINT-480691.
STRINGi 4932.YGR013W.

Proteomic databases

MaxQBi P53207.
PaxDbi P53207.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR013W ; YGR013W ; YGR013W .
GeneIDi 852896.
KEGGi sce:YGR013W.

Organism-specific databases

CYGDi YGR013w.
SGDi S000003245. SNU71.

Phylogenomic databases

eggNOGi NOG311014.
HOGENOMi HOG000154360.
OMAi ANDTIDQ.
OrthoDBi EOG7V76G7.

Enzyme and pathway databases

BioCyci YEAST:G3O-30740-MONOMER.

Miscellaneous databases

NextBioi 972568.

Gene expression databases

Genevestigatori P53207.

Family and domain databases

Gene3Di 1.20.1390.10. 1 hit.
InterProi IPR002483. PWI_dom.
[Graphical view ]
SMARTi SM00311. PWI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins."
    Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.
    RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-38; 104-128; 191-197; 232-240; 451-461; 468-479; 505-549 AND 597-613, IDENTIFICATION IN U1 SNRNP COMPLEX.
  5. "A generic protein purification method for protein complex characterization and proteome exploration."
    Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., Seraphin B.
    Nat. Biotechnol. 17:1030-1032(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "A role for the Psi-U mismatch in the recognition of the 5' splice site of yeast introns by the U1 small nuclear ribonucleoprotein particle."
    Libri D., Duconge F., Levy L., Vinauger M.
    J. Biol. Chem. 277:18173-18181(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE U1 SNRNP COMPLEX.
  7. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
    Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
    Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512 AND SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.

Entry informationi

Entry nameiSNU71_YEAST
AccessioniPrimary (citable) accession number: P53207
Secondary accession number(s): D6VUE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 782 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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