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P53207

- SNU71_YEAST

UniProt

P53207 - SNU71_YEAST

Protein

U1 small nuclear ribonucleoprotein component SNU71

Gene

SNU71

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: SGD

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30740-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U1 small nuclear ribonucleoprotein component SNU71
    Gene namesi
    Name:SNU71
    Ordered Locus Names:YGR013W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR013w.
    SGDiS000003245. SNU71.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. U1 snRNP Source: SGD
    3. U2-type prespliceosome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 620620U1 small nuclear ribonucleoprotein component SNU71PRO_0000202781Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei512 – 5121Phosphoserine1 Publication
    Modified residuei514 – 5141Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53207.
    PaxDbiP53207.

    Expressioni

    Gene expression databases

    GenevestigatoriP53207.

    Interactioni

    Subunit structurei

    Component of the 18S U1 snRNP particle, a subcomplex of the spliceosome.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP40P332035EBI-736,EBI-701

    Protein-protein interaction databases

    BioGridi33256. 51 interactions.
    DIPiDIP-5507N.
    IntActiP53207. 24 interactions.
    MINTiMINT-480691.
    STRINGi4932.YGR013W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53207.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili296 – 38590Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SNU71 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG311014.
    HOGENOMiHOG000154360.
    OMAiANDTIDQ.
    OrthoDBiEOG7V76G7.

    Family and domain databases

    Gene3Di1.20.1390.10. 1 hit.
    InterProiIPR002483. PWI_dom.
    [Graphical view]
    SMARTiSM00311. PWI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53207-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRDIVFVSPQ LYLSSQEGWK SDSAKSGFIP ILKNDLQRFQ DSLKHIVDAR    50
    NSLSETLLNS NDDGSIHNSD QNTGLNKDKE ASIADNNSAN KCATSSSRYQ 100
    ELKQFLPISL DQQIHTVSLQ GVSSSFSRGQ IESLLDHCLN LALTETQSNS 150
    ALKVEAWSSF SSFLDTQDIF IRFSKVDEDE AFVNTLNYCK ALFAFIRKLH 200
    EDFKIELHLD LNTKEYVEDR TGTIPSVKPE KASEFYSVFK NIEDQTDERN 250
    SKKEQLDDSS TQYKVDTNTL SDLPSDALDQ LCKDIIEFRT KVVSIEKEKK 300
    MKSTYEESRR QRHQMQKVFD QIRKNHSGAK GSANTEEEDT NMEDEDEEDD 350
    TEDDLALEKR KEERDLEESN RRYEDMLHQL HSNTEPKIKS IRADIMSAEN 400
    YEEHLEKNRS LYLKELLHLA NDVHYDHHRS FKEQEERRDE EDRAKNGNAK 450
    ELAPIQLSDG KAISAGKAAA ITLPEGTVKS ENYNADKNVS ESSEHVKIKF 500
    DFKKAIDHSV ESSSEDEGYR ESELPPTKPS ERSAAEDRLP FTADELNIRL 550
    TNLKESRYVD ELVREFLGVY EDELVEYILE NIRVNQSKQA LLNELRETFD 600
    EDGETIADRL WSRKEFRLGT 620
    Length:620
    Mass (Da):71,383
    Last modified:October 1, 1996 - v1
    Checksum:i2659AAA62C4993D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72798 Genomic DNA. Translation: CAA96996.1.
    BK006941 Genomic DNA. Translation: DAA08110.1.
    PIRiS64304.
    RefSeqiNP_011527.1. NM_001181142.1.

    Genome annotation databases

    EnsemblFungiiYGR013W; YGR013W; YGR013W.
    GeneIDi852896.
    KEGGisce:YGR013W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72798 Genomic DNA. Translation: CAA96996.1 .
    BK006941 Genomic DNA. Translation: DAA08110.1 .
    PIRi S64304.
    RefSeqi NP_011527.1. NM_001181142.1.

    3D structure databases

    ProteinModelPortali P53207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33256. 51 interactions.
    DIPi DIP-5507N.
    IntActi P53207. 24 interactions.
    MINTi MINT-480691.
    STRINGi 4932.YGR013W.

    Proteomic databases

    MaxQBi P53207.
    PaxDbi P53207.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR013W ; YGR013W ; YGR013W .
    GeneIDi 852896.
    KEGGi sce:YGR013W.

    Organism-specific databases

    CYGDi YGR013w.
    SGDi S000003245. SNU71.

    Phylogenomic databases

    eggNOGi NOG311014.
    HOGENOMi HOG000154360.
    OMAi ANDTIDQ.
    OrthoDBi EOG7V76G7.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30740-MONOMER.

    Miscellaneous databases

    NextBioi 972568.

    Gene expression databases

    Genevestigatori P53207.

    Family and domain databases

    Gene3Di 1.20.1390.10. 1 hit.
    InterProi IPR002483. PWI_dom.
    [Graphical view ]
    SMARTi SM00311. PWI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
      Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
      Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins."
      Gottschalk A., Tang J., Puig O., Salgado J., Neubauer G., Colot H.V., Mann M., Seraphin B., Rosbash M., Luehrmann R., Fabrizio P.
      RNA 4:374-393(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-38; 104-128; 191-197; 232-240; 451-461; 468-479; 505-549 AND 597-613, IDENTIFICATION IN U1 SNRNP COMPLEX.
    5. "A generic protein purification method for protein complex characterization and proteome exploration."
      Rigaut G., Shevchenko A., Rutz B., Wilm M., Mann M., Seraphin B.
      Nat. Biotechnol. 17:1030-1032(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1 SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "A role for the Psi-U mismatch in the recognition of the 5' splice site of yeast introns by the U1 small nuclear ribonucleoprotein particle."
      Libri D., Duconge F., Levy L., Vinauger M.
      J. Biol. Chem. 277:18173-18181(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE U1 SNRNP COMPLEX.
    7. "Composition and functional characterization of the yeast spliceosomal penta-snRNP."
      Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J.
      Mol. Cell 9:31-44(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN U1.U2.U4/U6.U5 PENTA-SNRNP COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512 AND SER-514, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.

    Entry informationi

    Entry nameiSNU71_YEAST
    AccessioniPrimary (citable) accession number: P53207
    Secondary accession number(s): D6VUE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 782 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3