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Protein

Putative cysteine synthase

Gene

YGR012W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Protein MET17 (MET17), Putative cysteine synthase (YGR012W)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei338 – 3381Pyridoxal phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cysteine synthase (EC:2.5.1.47)
Short name:
CSase
Alternative name(s):
O-acetylserine (thiol)-lyase
Short name:
OAS-TL
O-acetylserine sulfhydrylase
Gene namesi
Ordered Locus Names:YGR012W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR012W.
SGDiS000003244. YGR012W.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Putative cysteine synthasePRO_0000167130Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiP53206.
PeptideAtlasiP53206.

PTM databases

iPTMnetiP53206.

Interactioni

Protein-protein interaction databases

BioGridi33255. 56 interactions.
IntActiP53206. 1 interaction.
MINTiMINT-2492937.

Structurei

3D structure databases

ProteinModelPortaliP53206.
SMRiP53206. Positions 42-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni230 – 2345Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00730000111928.
HOGENOMiHOG000217393.
InParanoidiP53206.
KOiK01738.
OMAiRHQVDTL.
OrthoDBiEOG77DJGD.

Family and domain databases

InterProiIPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSQNKTSV SLAWRECISI ASVLIGAYAS YKYYKLFKTR DIPRPKEGVE
60 70 80 90 100
ELIGNTPLVK IRSLTKATGV NIYAKLELCN PAGSAKDRVA LNIIKTAEEL
110 120 130 140 150
GELVRGEPGW VFEGTSGSTG ISIAVVCNAL GYRAHISLPD DTSLEKLALL
160 170 180 190 200
ESLGATVNKV KPASIVDPNQ YVNAAKKACN ELKKSGNGIR AVFADQFENE
210 220 230 240 250
ANWKVHYQTT GPEIAHQTKG NIDAFIAGCG TGGTITGVAK FLKERAKIPC
260 270 280 290 300
HVVLADPQGS GFYNRVNYGV MYDYVEKEGT RRRHQVDTIV EGIGLNRITH
310 320 330 340 350
NFHMGEKFID ESIRVNDNQA IRMAKYLSVN DGLFVGSSTA INAVAAIQVA
360 370 380 390
KTLPHGSNIV IIACDSGSRH LSKFWKEAKE IDHDVSLEEV INI
Length:393
Mass (Da):42,801
Last modified:October 1, 1996 - v1
Checksum:i76C73396D77B69AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72797 Genomic DNA. Translation: CAA96995.1.
BK006941 Genomic DNA. Translation: DAA08109.1.
PIRiS64303.
RefSeqiNP_011526.1. NM_001181141.1.

Genome annotation databases

EnsemblFungiiYGR012W; YGR012W; YGR012W.
GeneIDi852895.
KEGGisce:YGR012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72797 Genomic DNA. Translation: CAA96995.1.
BK006941 Genomic DNA. Translation: DAA08109.1.
PIRiS64303.
RefSeqiNP_011526.1. NM_001181141.1.

3D structure databases

ProteinModelPortaliP53206.
SMRiP53206. Positions 42-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33255. 56 interactions.
IntActiP53206. 1 interaction.
MINTiMINT-2492937.

PTM databases

iPTMnetiP53206.

Proteomic databases

MaxQBiP53206.
PeptideAtlasiP53206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR012W; YGR012W; YGR012W.
GeneIDi852895.
KEGGisce:YGR012W.

Organism-specific databases

EuPathDBiFungiDB:YGR012W.
SGDiS000003244. YGR012W.

Phylogenomic databases

GeneTreeiENSGT00730000111928.
HOGENOMiHOG000217393.
InParanoidiP53206.
KOiK01738.
OMAiRHQVDTL.
OrthoDBiEOG77DJGD.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.

Miscellaneous databases

NextBioi972565.
PROiP53206.

Family and domain databases

InterProiIPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCYSK_YEAST
AccessioniPrimary (citable) accession number: P53206
Secondary accession number(s): D6VUE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4650 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.