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Protein

Putative cysteine synthase

Gene

YGR012W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Putative cysteine synthase that catalyzes the conversion of O-acetyl-L-serine (OAS) into cysteine, the last step in the cysteine biosynthesis pathway. However, this CS-like protein is unlikely to function in cysteine biosynthesis. It seems that in S.cerevisiae cysteine biosynthesis occurs exclusively through the cystathionine pathway and not via direct incorporation of sulfur into OAS.1 Publication

Miscellaneous

Present with 4650 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei338Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cysteine synthase1 Publication (EC:2.5.1.47)
Short name:
CS
Alternative name(s):
Cysteine synthase-like protein1 Publication
Short name:
CSl
O-acetylserine (thiol)-lyase
Short name:
OAS-TL
O-acetylserine sulfhydrylase
Gene namesi
Ordered Locus Names:YGR012WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR012W
SGDiS000003244 YGR012W

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 31HelicalSequence analysisAdd BLAST20

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671301 – 393Putative cysteine synthaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiP53206
PaxDbiP53206
PRIDEiP53206

PTM databases

iPTMnetiP53206

Interactioni

Protein-protein interaction databases

BioGridi33255127 interactors.
IntActiP53206 3 interactors.
MINTiP53206
STRINGi4932.YGR012W

Structurei

3D structure databases

ProteinModelPortaliP53206
SMRiP53206
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 234Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00890000139878
HOGENOMiHOG000217393
InParanoidiP53206
KOiK01738
OMAiGIRRWPE
OrthoDBiEOG092C33M9

Family and domain databases

InterProiView protein in InterPro
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
PROSITEiView protein in PROSITE
PS00901 CYS_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P53206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSQNKTSV SLAWRECISI ASVLIGAYAS YKYYKLFKTR DIPRPKEGVE
60 70 80 90 100
ELIGNTPLVK IRSLTKATGV NIYAKLELCN PAGSAKDRVA LNIIKTAEEL
110 120 130 140 150
GELVRGEPGW VFEGTSGSTG ISIAVVCNAL GYRAHISLPD DTSLEKLALL
160 170 180 190 200
ESLGATVNKV KPASIVDPNQ YVNAAKKACN ELKKSGNGIR AVFADQFENE
210 220 230 240 250
ANWKVHYQTT GPEIAHQTKG NIDAFIAGCG TGGTITGVAK FLKERAKIPC
260 270 280 290 300
HVVLADPQGS GFYNRVNYGV MYDYVEKEGT RRRHQVDTIV EGIGLNRITH
310 320 330 340 350
NFHMGEKFID ESIRVNDNQA IRMAKYLSVN DGLFVGSSTA INAVAAIQVA
360 370 380 390
KTLPHGSNIV IIACDSGSRH LSKFWKEAKE IDHDVSLEEV INI
Length:393
Mass (Da):42,801
Last modified:October 1, 1996 - v1
Checksum:i76C73396D77B69AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72797 Genomic DNA Translation: CAA96995.1
BK006941 Genomic DNA Translation: DAA08109.1
PIRiS64303
RefSeqiNP_011526.1, NM_001181141.1

Genome annotation databases

EnsemblFungiiYGR012W; YGR012W; YGR012W
GeneIDi852895
KEGGisce:YGR012W

Similar proteinsi

Entry informationi

Entry nameiCYSKL_YEAST
AccessioniPrimary (citable) accession number: P53206
Secondary accession number(s): D6VUE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome