P53206 (CYSK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Ordered Locus Names:

YGR012W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Miscellaneous	Present with 4650 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial outer membrane Inferred from direct assay PubMed 16407407. Source: SGD
Molecular_function	cysteine synthase activity Inferred from sequence or structural similarity PubMed 10592175. Source: SGD transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Putative cysteine synthase		PRO_0000167130
Regions
	Region	230 – 234	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	338	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	86	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53206 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 76C73396D77B69AC	FASTA	393	42,801
	10 20 30 40 50 60 MSCSQNKTSV SLAWRECISI ASVLIGAYAS YKYYKLFKTR DIPRPKEGVE ELIGNTPLVK 70 80 90 100 110 120 IRSLTKATGV NIYAKLELCN PAGSAKDRVA LNIIKTAEEL GELVRGEPGW VFEGTSGSTG 130 140 150 160 170 180 ISIAVVCNAL GYRAHISLPD DTSLEKLALL ESLGATVNKV KPASIVDPNQ YVNAAKKACN 190 200 210 220 230 240 ELKKSGNGIR AVFADQFENE ANWKVHYQTT GPEIAHQTKG NIDAFIAGCG TGGTITGVAK 250 260 270 280 290 300 FLKERAKIPC HVVLADPQGS GFYNRVNYGV MYDYVEKEGT RRRHQVDTIV EGIGLNRITH 310 320 330 340 350 360 NFHMGEKFID ESIRVNDNQA IRMAKYLSVN DGLFVGSSTA INAVAAIQVA KTLPHGSNIV 370 380 390 IIACDSGSRH LSKFWKEAKE IDHDVSLEEV INI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72797 Genomic DNA. Translation: CAA96995.1. BK006941 Genomic DNA. Translation: DAA08109.1.
PIR	S64303.
RefSeq	NP_011526.1. NM_001181141.1.
3D structure databases
ProteinModelPortal	P53206.
SMR	P53206. Positions 42-372.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53206. 1 interaction.
MINT	MINT-2492937.
STRING	4932.YGR012W.
Proteomic databases
PaxDb	P53206.
PeptideAtlas	P53206.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR012W; YGR012W; YGR012W.
GeneID	852895.
KEGG	sce:YGR012W.
Organism-specific databases
CYGD	YGR012w.
SGD	S000003244. YGR012W.
Phylogenomic databases
eggNOG	COG0031.
GeneTree	ENSGT00700000105450.
HOGENOM	HOG000217393.
KO	K01738.
OMA	VAPAYGC.
OrthoDB	EOG45MRFC.
Enzyme and pathway databases
UniPathway	UPA00136; UER00200.
Gene expression databases
ArrayExpress	P53206.
Genevestigator	P53206.
GermOnline	YGR012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972565.

Entry information

Entry name

CYSK_YEAST

Accession

Primary (citable) accession number: P53206
Secondary accession number(s): D6VUE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program