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Protein

Putative cysteine synthase

Gene

YGR012W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Putative cysteine synthase that catalyzes the conversion of O-acetyl-L-serine (OAS) into cysteine, the last step in the cysteine biosynthesis pathway. However, this CS-like protein is unlikely to function in cysteine biosynthesis. It seems that in S.cerevisiae cysteine biosynthesis occurs exclusively through the cystathionine pathway and not via direct incorporation of sulfur into OAS.1 Publication

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.By similarity

Cofactori

pyridoxal 5'-phosphateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei338Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cysteine synthase1 Publication (EC:2.5.1.47)
Short name:
CS
Alternative name(s):
Cysteine synthase-like protein1 Publication
Short name:
CSl
O-acetylserine (thiol)-lyase
Short name:
OAS-TL
O-acetylserine sulfhydrylase
Gene namesi
Ordered Locus Names:YGR012WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR012W.
SGDiS000003244. YGR012W.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 31HelicalSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671301 – 393Putative cysteine synthaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiP53206.
PRIDEiP53206.

PTM databases

iPTMnetiP53206.

Interactioni

Protein-protein interaction databases

BioGridi33255. 56 interactors.
IntActiP53206. 1 interactor.
MINTiMINT-2492937.

Structurei

3D structure databases

ProteinModelPortaliP53206.
SMRiP53206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 234Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000134233.
HOGENOMiHOG000217393.
InParanoidiP53206.
KOiK01738.
OMAiGVMYDSV.
OrthoDBiEOG092C33M9.

Family and domain databases

InterProiIPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCSQNKTSV SLAWRECISI ASVLIGAYAS YKYYKLFKTR DIPRPKEGVE
60 70 80 90 100
ELIGNTPLVK IRSLTKATGV NIYAKLELCN PAGSAKDRVA LNIIKTAEEL
110 120 130 140 150
GELVRGEPGW VFEGTSGSTG ISIAVVCNAL GYRAHISLPD DTSLEKLALL
160 170 180 190 200
ESLGATVNKV KPASIVDPNQ YVNAAKKACN ELKKSGNGIR AVFADQFENE
210 220 230 240 250
ANWKVHYQTT GPEIAHQTKG NIDAFIAGCG TGGTITGVAK FLKERAKIPC
260 270 280 290 300
HVVLADPQGS GFYNRVNYGV MYDYVEKEGT RRRHQVDTIV EGIGLNRITH
310 320 330 340 350
NFHMGEKFID ESIRVNDNQA IRMAKYLSVN DGLFVGSSTA INAVAAIQVA
360 370 380 390
KTLPHGSNIV IIACDSGSRH LSKFWKEAKE IDHDVSLEEV INI
Length:393
Mass (Da):42,801
Last modified:October 1, 1996 - v1
Checksum:i76C73396D77B69AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72797 Genomic DNA. Translation: CAA96995.1.
BK006941 Genomic DNA. Translation: DAA08109.1.
PIRiS64303.
RefSeqiNP_011526.1. NM_001181141.1.

Genome annotation databases

EnsemblFungiiYGR012W; YGR012W; YGR012W.
GeneIDi852895.
KEGGisce:YGR012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72797 Genomic DNA. Translation: CAA96995.1.
BK006941 Genomic DNA. Translation: DAA08109.1.
PIRiS64303.
RefSeqiNP_011526.1. NM_001181141.1.

3D structure databases

ProteinModelPortaliP53206.
SMRiP53206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33255. 56 interactors.
IntActiP53206. 1 interactor.
MINTiMINT-2492937.

PTM databases

iPTMnetiP53206.

Proteomic databases

MaxQBiP53206.
PRIDEiP53206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR012W; YGR012W; YGR012W.
GeneIDi852895.
KEGGisce:YGR012W.

Organism-specific databases

EuPathDBiFungiDB:YGR012W.
SGDiS000003244. YGR012W.

Phylogenomic databases

GeneTreeiENSGT00860000134233.
HOGENOMiHOG000217393.
InParanoidiP53206.
KOiK01738.
OMAiGVMYDSV.
OrthoDBiEOG092C33M9.

Miscellaneous databases

PROiP53206.

Family and domain databases

InterProiIPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSKL_YEAST
AccessioniPrimary (citable) accession number: P53206
Secondary accession number(s): D6VUE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4650 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.