Reviewed, UniProtKB/Swiss-Prot P53204 (NMA2_YEAST)

Names and origin

Protein names

Recommended name:
    Nicotinamide-nucleotide adenylyltransferase 2
    EC=2.7.7.1
Alternative name(s):
    NAD(+) pyrophosphorylase 2
    NAD(+) diphosphorylase 2
    NMN adenylyltransferase 2

Gene names

Name:	NMA2
Ordered Locus Names:	YGR010W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + nicotinamide ribonucleotide = diphosphate + NAD+.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
Subcellular location	Nucleus Ref.2.
Miscellaneous	Present with 1430 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the eukaryotic NMN adenylyltransferase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Nucleus
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nicotinamide-nucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC nicotinate-nucleotide adenylyltransferase activity Ref.2 Inferred from genetic interaction. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NMA1	Q06178	3	EBI-23073,EBI-11803
SRP1	Q02821	1	EBI-23073,EBI-1797

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Nicotinamide-nucleotide adenylyltransferase 2		PRO_0000135019
Amino acid modifications
	Modified residue	85	1	Phosphoserine Ref.4 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53204-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 4A358AF7885B6568	FASTA	395	44,909
	10 20 30 40 50 60 MDPTKAPDFK PPQPNEELQP PPDPTHTIPK SGPIVPYVLA DYNSSIDAPF NLDIYKTLSS 70 80 90 100 110 120 RKKNANSSNR MDHIPLNTSD FQPLSRDVSS EEESEGQSNG IDATLQDVTM TGNLGVLKSQ 130 140 150 160 170 180 IADLEEVPHT IVRQARTIED YEFPVHRLTK KLQDPEKLPL IIVACGSFSP ITYLHLRMFE 190 200 210 220 230 240 MALDDINEQT RFEVVGGYFS PVSDNYQKRG LAPAYHRVRM CELACERTSS WLMVDAWESL 250 260 270 280 290 300 QSSYTRTAKV LDHFNHEINI KRGGIMTVDG EKMGVKIMLL AGGDLIESMG EPHVWADSDL 310 320 330 340 350 360 HHILGNYGCL IVERTGSDVR SFLLSHDIMY EHRRNILIIK QLIYNDISST KVRLFIRRGM 370 380 390 SVQYLLPNSV IRYIQEYNLY INQSEPVKQV LDSKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Manipulation of a nuclear NAD+ salvage pathway delays aging without altering steady-state NAD+ levels." Anderson R.M., Bitterman K.J., Wood J.G., Medvedik O., Cohen H., Lin S.S., Manchester J.K., Gordon J.I., Sinclair D.A. J. Biol. Chem. 277:18881-18890(2002) [PubMed: 11884393] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72795 Genomic DNA. Translation: CAA96993.1.
PIR	S64299.
RefSeq	NP_011524.1.
3D structure databases
SMR	P53204. Positions 157-381.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1227N.
IntAct	P53204. 7 interactions.
STRING	P53204.
Genome annotation databases
Ensembl	YGR010W; YGR010W; YGR010W; Saccharomyces cerevisiae. [Genome view]
GeneID	852893.
KEGG	sce:YGR010W.
NMPDR	fig|4932.3.peg.2634.
Organism-specific databases
CYGD	YGR010w.
SGD	S000003242. NMA2.
Phylogenomic databases
eggNOG	fuNOG07710.
HOGENOM	HBG397934.
OMA	RGVKIML.
OrthoDB	EOG9JWWWT.
PhylomeDB	P53204.
Enzyme and pathway databases
BioCyc	MetaCyc:YGR010W-MONOMER.
BRENDA	2.7.7.1. 250.
Gene expression databases
ArrayExpress	P53204.
Genevestigator	P53204.
GermOnline	YGR010W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004820. Cytidylyltransf. IPR005248. NAMN_adtrnsfrase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR12039. NAMN_adtrnsfrase. 1 hit.
Pfam	PF01467. CTP_transf_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00482. NAMN_adtrnsfrase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	972562.

Entry information

Entry name

NMA2_YEAST

Accession

Primary (citable) accession number: P53204

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)