P53203 (PEX31_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal membrane protein PEX31

Alternative name(s):
Peroxin-31

Gene names

Name:	PEX31
Ordered Locus Names:	YGR004W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Peroxisome membrane; Multi-pass membrane protein Ref.3.
Miscellaneous	Present with 238 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PEX28-32 family. PEX30/31 subfamily.

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peroxisome organization Inferred from mutant phenotype PubMed 14617799. Source: SGD
Cellular_component	integral to peroxisomal membrane Inferred from direct assay PubMed 14617799. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Peroxisomal membrane protein PEX31		PRO_0000202779
Regions
	Topological domain	1 – 90	90	Cytoplasmic Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Topological domain	112 – 175	64	Peroxisomal Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Topological domain	197 – 462	266	Cytoplasmic Potential
Amino acid modifications
	Modified residue	432	1	Phosphoserine Ref.6 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P53203 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3F875D40748AB6C3	FASTA	462	52,943
	10 20 30 40 50 60 MSEINNENLE PTSSTVAEST ESKNKHIRSA LRKRRGKLSA QTYEEDQEAI LSSPLLTSTP 70 80 90 100 110 120 KTVSRSLVRL YPYLIVVDNF LSIITWSNDN VSANLLGIFL FTVCVLYFGF ITRYFGHLMI 130 140 150 160 170 180 VGIIWVYLLI DKHVQETMAS CPSLDDIIHV MDRVSMKSSA VLSPITILSA QDVRRLLFTI 190 200 210 220 230 240 AFLSPVYIFL TVFVLSPNYL MLIGGLYVLT YHSKLIRRMR RYLWKFRVVR LLVFFITGLD 250 260 270 280 290 300 LGGPDNNRRL FASVNKKIRS FVWNEVGNTS NTKKTVLFKV ALFENQRRWL GIGWTSTMLS 310 320 330 340 350 360 YERASWTDEF LNTSPSPEVF TLPEEQSGMA WEWHDKDWML DLTNDGIIQL PASAAKTKVK 370 380 390 400 410 420 PGADEGFIYY DNTWNNPSAT DTYKKYTRRR RWIRTATVTT TYDDEPTVEK ATPNSHALKS 430 440 450 460 EENNRVRKRK VSFSTANEVH IIPSSDSSKL IQISDVSMSP SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-432, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72789 Genomic DNA. Translation: CAA96987.1. BK006941 Genomic DNA. Translation: DAA08102.1.
PIR	S64293.
RefSeq	NP_011518.1. NM_001181133.1.
3D structure databases
ProteinModelPortal	P53203.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5455N.
IntAct	P53203. 2 interactions.
MINT	MINT-480826.
STRING	4932.YGR004W.
Proteomic databases
PaxDb	P53203.
PRIDE	P53203.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR004W; YGR004W; YGR004W.
GeneID	852887.
KEGG	sce:YGR004W.
Organism-specific databases
CYGD	YGR004w.
SGD	S000003236. PEX31.
Phylogenomic databases
eggNOG	NOG72309.
GeneTree	ENSGT00390000017777.
HOGENOM	HOG000180096.
OrthoDB	EOG4ZSDBV.
Gene expression databases
Genevestigator	P53203.
GermOnline	YGR004W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010482. Peroxin/Dysferlin. IPR006614. Peroxin/Ferlin. [Graphical view]
Pfam	PF06398. Pex24p. 1 hit. [Graphical view]
SMART	SM00694. DysFC. 1 hit. SM00693. DysFN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972544.

Entry information

Entry name

PEX31_YEAST

Accession

Primary (citable) accession number: P53203
Secondary accession number(s): D6VUE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program