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Protein

Cullin-3

Gene

CUL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of a ubiquitin-protein ligase. Together with ELA1 is required for polyubiquitination and degradation of RBP1.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30734-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cullin-3
Alternative name(s):
Cullin-B
Gene namesi
Name:CUL3
Ordered Locus Names:YGR003W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR003W.
SGDiS000003235. CUL3.

Subcellular locationi

GO - Cellular componenti

  • Cul3-RING ubiquitin ligase complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198061 – 744Cullin-3Add BLAST744

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki688Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8)By similarity

Post-translational modificationi

Neddylated; enhancing the ubiquitin-ligase activity.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP53202.
PRIDEiP53202.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33247. 14 interactors.
DIPiDIP-5506N.
IntActiP53202. 3 interactors.
MINTiMINT-531464.

Structurei

3D structure databases

ProteinModelPortaliP53202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000132044.
HOGENOMiHOG000112081.
InParanoidiP53202.
KOiK03869.
OMAiIEQSTAI.
OrthoDBiEOG092C1L4K.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITNKKIKIS VPEKLGLSEE SFEESWETVK YAIDHIYSDD MADLSFEQVY
60 70 80 90 100
KTIYTIVLNK KGPILYNRLK DYLIQKLALL RETIVKDNTH DYEFLGTMAR
110 120 130 140 150
LWEVQCHCFK ITGDLMMYMD KVYCKPNRCM EVYDMCLDLF RIEILQKCSS
160 170 180 190 200
SLISALISDI ERIRNLGSVD SEHTSLWKVL IGMMETLHDN RDSFFLTDFE
210 220 230 240 250
PVLISATEEY YNKAIDIELL TPIESLEKIR KLRQFESMLD SSFLNVDSHN
260 270 280 290 300
KLKTVLENVL IWGKLSDIIE DLTHEAMVIS NGKLLQEIYD LSSEEKYRVT
310 320 330 340 350
VIESIKSYIN KNAINIPFNE GNRKKGQNAI TWSSEIVELY RSQHSFLESI
360 370 380 390 400
DFGSVRLNNL TGDVSNAILG DVFSMYFSKE GALPSEYLST YVDHCMKRTK
410 420 430 440 450
EKDAEIVKIK QDLLDSTKLI GLLTEKDIFE KIYKKQLSRR LLQQRSLVEI
460 470 480 490 500
EKWMVQMIKK VLGTFFTSKL EIMLRDISLS SEMYQAFQHS TINSIEYLSF
510 520 530 540 550
APQVLTRTSW PFQSTNPIDE GISLPPRMSQ ILAGFEGYYS LKYKERVLKW
560 570 580 590 600
AHHLSVIEIG CQFNSGYYEI SFSVYAGVIF LLFEDYEELT LGEIYELTHI
610 620 630 640 650
PIDDVKSLVM SMSTIPRCKI LKKSSSSGNM KFSVNYFFSS PNRKVKVPVI
660 670 680 690 700
ACPLPSQKSD NLATASSVDT YDNEIVMELS AIIVRIMKTE GKLSHQQLLE
710 720 730 740
RTTKRTQSRL SLTPSILKRS IQLLIEKEYI QRNADDPSYY HYLS
Length:744
Mass (Da):86,115
Last modified:October 1, 1996 - v1
Checksum:i6C4EB4A70F3CB18F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY387706 Genomic DNA. Translation: AAQ91375.1.
Z72788 Genomic DNA. Translation: CAA96986.1.
BK006941 Genomic DNA. Translation: DAA08101.1.
PIRiS64292.
RefSeqiNP_011517.1. NM_001181132.1.

Genome annotation databases

EnsemblFungiiYGR003W; YGR003W; YGR003W.
GeneIDi852886.
KEGGisce:YGR003W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY387706 Genomic DNA. Translation: AAQ91375.1.
Z72788 Genomic DNA. Translation: CAA96986.1.
BK006941 Genomic DNA. Translation: DAA08101.1.
PIRiS64292.
RefSeqiNP_011517.1. NM_001181132.1.

3D structure databases

ProteinModelPortaliP53202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33247. 14 interactors.
DIPiDIP-5506N.
IntActiP53202. 3 interactors.
MINTiMINT-531464.

Proteomic databases

MaxQBiP53202.
PRIDEiP53202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR003W; YGR003W; YGR003W.
GeneIDi852886.
KEGGisce:YGR003W.

Organism-specific databases

EuPathDBiFungiDB:YGR003W.
SGDiS000003235. CUL3.

Phylogenomic databases

GeneTreeiENSGT00840000132044.
HOGENOMiHOG000112081.
InParanoidiP53202.
KOiK03869.
OMAiIEQSTAI.
OrthoDBiEOG092C1L4K.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-30734-MONOMER.

Miscellaneous databases

PROiP53202.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR019559. Cullin_neddylation_domain.
IPR016159. Cullin_repeat-like_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00888. Cullin. 1 hit.
PF10557. Cullin_Nedd8. 1 hit.
[Graphical view]
SMARTiSM00182. CULLIN. 1 hit.
SM00884. Cullin_Nedd8. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF74788. SSF74788. 1 hit.
SSF75632. SSF75632. 1 hit.
PROSITEiPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUL3_YEAST
AccessioniPrimary (citable) accession number: P53202
Secondary accession number(s): D6VUE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.