Reviewed, UniProtKB/Swiss-Prot P53201 (SWC4_YEAST)

Names and origin

Protein names

Recommended name:
    SWR1-complex protein 4
Alternative name(s):
    ESA1-associated factor 2

Gene names

Name:	SWC4
Synonyms:	EAF2, GOD1
Ordered Locus Names:	YGR002C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Ref.3 Ref.9 Ref.10
Subunit structure	Component of the SWR1 chromatin-remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2. Interacts with YAF9. Ref.6
Subcellular location	Nucleus. Ref.4
Miscellaneous	Present with 2230 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the SWC4 family. Contains 1 SANT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Chromatin regulator
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay. Source: SGD establishment of mitotic sister chromatid cohesion Inferred from mutant phenotype. Source: SGD histone acetylation Inferred from physical interaction. Source: SGD histone exchange Inferred from physical interaction. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	NuA4 histone acetyltransferase complex Ref.7 Ref.8 Inferred from physical interaction. Source: SGD Swr1 complex Ref.3 Ref.10 Inferred from direct assay. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro protein binding Ref.9 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BDF1	P35817	1	EBI-23061,EBI-3493
ESA1	Q08649	1	EBI-23061,EBI-6648
RVB2	Q12464	1	EBI-23061,EBI-31814
SWR1	Q05471	1	EBI-23061,EBI-22102
TRA1	P38811	1	EBI-23061,EBI-24638
VID21	Q06337	1	EBI-23061,EBI-35867
YAF9	P53930	1	EBI-23061,EBI-28841

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	SWR1-complex protein 4		PRO_0000072345
Regions
	Domain	156 – 209	54	SANT

Sequences

	Sequence		Length	Mass (Da)
	P53201-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 512CB8DF3171E7A3	FASTA	476	55,213
	10 20 30 40 50 60 MSSSDIFDVL NIKQKSRSPT NGQVSVPSSS AANRPKPQVT GMQRELFNLL GENQPPVVIK 70 80 90 100 110 120 SGNNFKEKML STSKPSPWSF VEFKANNSVT LRHWVKGSKE LIGDTPKESP YSKFNQHLSI 130 140 150 160 170 180 PSFTKEEYEA FMNENEGTQK SVESEKNHNE NFTNEKKDES KNSWSFEEIE YLFNLCKKYD 190 200 210 220 230 240 LRWFLIFDRY SYNNSRTLED LKEKFYYTCR NYFKASDPSN PLLSSLNFSA EKEIERKKYL 250 260 270 280 290 300 QRLLSRSAAE IAEEEALVVE SKKFEMAAKR TLAERESLLR LLDSPHSDQT ITQYLTSQGM 310 320 330 340 350 360 SQLYNALLAD KTRKRKHDLN IPENPWMKQQ QQFAQHRQLQ QLNVKKSEVK ENLSPKKTKR 370 380 390 400 410 420 QRQEMQTALK RKSESAYAEQ LLKDFNSDER KALGVITHGE KLSPGVYLRS TKLSTFKPAL 430 440 450 460 470 QNKILAILQE LSLPSRPVMP SFDVMERQEE LLKKINTLID LKKHVDKYEA GMSITK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed: 14690608] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Direct physical and functional interaction of the NuA4 complex components Yaf9p and Swc4p." Bittner C.B., Zeisig D.T., Zeisig B.B., Slany R.K. Eukaryot. Cell 3:976-983(2004) [PubMed: 15302830] [Abstract] Cited for: INTERACTION WITH YAF9.
[7]	"The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres." Zhang H., Richardson D.O., Roberts D.N., Utley R.T., Erdjument-Bromage H., Tempst P., Cote J., Cairns B.R. Mol. Cell. Biol. 24:9424-9436(2004) [PubMed: 15485911] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
[8]	"Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4." Krogan N.J., Baetz K., Keogh M.-C., Datta N., Sawa C., Kwok T.C.Y., Thompson N.J., Davey M.G., Pootoolal J., Hughes T.R., Emili A., Buratowski S., Hieter P., Greenblatt J.F. Proc. Natl. Acad. Sci. U.S.A. 101:13513-13518(2004) [PubMed: 15353583] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
[9]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed: 15045029] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, MASS SPECTROMETRY.
[10]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed: 14645854] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72787 Genomic DNA. Translation: CAA96985.1. AY692656 Genomic DNA. Translation: AAT92675.1.
PIR	S64291.
RefSeq	NP_011516.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6510N.
IntAct	P53201. 35 interactions.
Proteomic databases
PeptideAtlas	P53201.
Genome annotation databases
Ensembl	YGR002C. Saccharomyces cerevisiae. [Contig view]
GeneID	852885.
GenomeReviews	Gene locus YGR002C in contig Y13135_GR.
KEGG	sce:YGR002C.
NMPDR	fig|4932.3.peg.2626.
Organism-specific databases
CYGD	YGR002c.
SGD	S000003234. SWC4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53201.
OMA	P53201. ARELYNL.
Gene expression databases
ArrayExpress	P53201.
GermOnline	YGR002C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014778. Myb_DNA-bd. IPR001005. SANT_DNA-bd. [Graphical view]
Pfam	PF00249. Myb_DNA-binding. 1 hit. [Graphical view]
SMART	SM00717. SANT. 1 hit. [Graphical view]
PROSITE	PS51293. SANT. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972538.

Entry information

Entry name

SWC4_YEAST

Accession

Primary (citable) accession number: P53201

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)