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Protein

SWR1-complex protein 4

Gene

SWC4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.3 Publications

GO - Molecular functioni

GO - Biological processi

  • chromatin modification Source: SGD
  • chromatin remodeling Source: SGD
  • chromosome organization Source: SGD
  • DNA repair Source: SGD
  • histone exchange Source: SGD
  • histone H2A acetylation Source: GO_Central
  • histone H4 acetylation Source: GO_Central
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30733-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SWR1-complex protein 4
Alternative name(s):
ESA1-associated factor 2
Gene namesi
Name:SWC4
Synonyms:EAF2, GOD1
Ordered Locus Names:YGR002C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR002C.
SGDiS000003234. SWC4.

Subcellular locationi

GO - Cellular componenti

  • histone acetyltransferase complex Source: SGD
  • NuA4 histone acetyltransferase complex Source: SGD
  • Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 476475SWR1-complex protein 4PRO_0000072345Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP53201.

Interactioni

Subunit structurei

Component of the SWR1 chromatin-remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2. Interacts with YAF9.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EAF1Q063374EBI-23061,EBI-35867
ESA1Q086498EBI-23061,EBI-6648
RVB2Q124643EBI-23061,EBI-31814
SWR1Q054716EBI-23061,EBI-22102
TRA1P388114EBI-23061,EBI-24638
YAF9P539305EBI-23061,EBI-28841

Protein-protein interaction databases

BioGridi33246. 212 interactions.
DIPiDIP-6510N.
IntActiP53201. 34 interactions.
MINTiMINT-675383.

Structurei

3D structure databases

ProteinModelPortaliP53201.
SMRiP53201. Positions 162-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini156 – 20954SANTAdd
BLAST

Sequence similaritiesi

Belongs to the SWC4 family.Curated
Contains 1 SANT domain.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016466.
HOGENOMiHOG000246708.
InParanoidiP53201.
KOiK11324.
OMAiNLYNNLM.
OrthoDBiEOG70ZZXC.

Family and domain databases

InterProiIPR032563. DAMP1_SANT_like.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR027109. Swc4/Dmap1.
[Graphical view]
PANTHERiPTHR12855. PTHR12855. 2 hits.
PfamiPF16282. SANT_DAMP1_like. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSDIFDVL NIKQKSRSPT NGQVSVPSSS AANRPKPQVT GMQRELFNLL
60 70 80 90 100
GENQPPVVIK SGNNFKEKML STSKPSPWSF VEFKANNSVT LRHWVKGSKE
110 120 130 140 150
LIGDTPKESP YSKFNQHLSI PSFTKEEYEA FMNENEGTQK SVESEKNHNE
160 170 180 190 200
NFTNEKKDES KNSWSFEEIE YLFNLCKKYD LRWFLIFDRY SYNNSRTLED
210 220 230 240 250
LKEKFYYTCR NYFKASDPSN PLLSSLNFSA EKEIERKKYL QRLLSRSAAE
260 270 280 290 300
IAEEEALVVE SKKFEMAAKR TLAERESLLR LLDSPHSDQT ITQYLTSQGM
310 320 330 340 350
SQLYNALLAD KTRKRKHDLN IPENPWMKQQ QQFAQHRQLQ QLNVKKSEVK
360 370 380 390 400
ENLSPKKTKR QRQEMQTALK RKSESAYAEQ LLKDFNSDER KALGVITHGE
410 420 430 440 450
KLSPGVYLRS TKLSTFKPAL QNKILAILQE LSLPSRPVMP SFDVMERQEE
460 470
LLKKINTLID LKKHVDKYEA GMSITK
Length:476
Mass (Da):55,213
Last modified:October 1, 1996 - v1
Checksum:i512CB8DF3171E7A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72787 Genomic DNA. Translation: CAA96985.1.
AY692656 Genomic DNA. Translation: AAT92675.1.
BK006941 Genomic DNA. Translation: DAA08100.1.
PIRiS64291.
RefSeqiNP_011516.1. NM_001181131.1.

Genome annotation databases

EnsemblFungiiYGR002C; YGR002C; YGR002C.
GeneIDi852885.
KEGGisce:YGR002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72787 Genomic DNA. Translation: CAA96985.1.
AY692656 Genomic DNA. Translation: AAT92675.1.
BK006941 Genomic DNA. Translation: DAA08100.1.
PIRiS64291.
RefSeqiNP_011516.1. NM_001181131.1.

3D structure databases

ProteinModelPortaliP53201.
SMRiP53201. Positions 162-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33246. 212 interactions.
DIPiDIP-6510N.
IntActiP53201. 34 interactions.
MINTiMINT-675383.

Proteomic databases

MaxQBiP53201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR002C; YGR002C; YGR002C.
GeneIDi852885.
KEGGisce:YGR002C.

Organism-specific databases

EuPathDBiFungiDB:YGR002C.
SGDiS000003234. SWC4.

Phylogenomic databases

GeneTreeiENSGT00390000016466.
HOGENOMiHOG000246708.
InParanoidiP53201.
KOiK11324.
OMAiNLYNNLM.
OrthoDBiEOG70ZZXC.

Enzyme and pathway databases

BioCyciYEAST:G3O-30733-MONOMER.

Miscellaneous databases

PROiP53201.

Family and domain databases

InterProiIPR032563. DAMP1_SANT_like.
IPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR027109. Swc4/Dmap1.
[Graphical view]
PANTHERiPTHR12855. PTHR12855. 2 hits.
PfamiPF16282. SANT_DAMP1_like. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Direct physical and functional interaction of the NuA4 complex components Yaf9p and Swc4p."
    Bittner C.B., Zeisig D.T., Zeisig B.B., Slany R.K.
    Eukaryot. Cell 3:976-983(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YAF9.
  8. "The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres."
    Zhang H., Richardson D.O., Roberts D.N., Utley R.T., Erdjument-Bromage H., Tempst P., Cote J., Cairns B.R.
    Mol. Cell. Biol. 24:9424-9436(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4."
    Krogan N.J., Baetz K., Keogh M.-C., Datta N., Sawa C., Kwok T.C.Y., Thompson N.J., Davey M.G., Pootoolal J., Hughes T.R., Emili A., Buratowski S., Hieter P., Greenblatt J.F.
    Proc. Natl. Acad. Sci. U.S.A. 101:13513-13518(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin."
    Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J.
    PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  11. "ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex."
    Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C.
    Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSWC4_YEAST
AccessioniPrimary (citable) accession number: P53201
Secondary accession number(s): D6VUD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.