SWR1-complex protein 4 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53201 (SWC4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
SWR1-complex protein 4

Alternative name(s):
ESA1-associated factor 2

Gene names

Name:	SWC4
Synonyms:	EAF2, GOD1
Ordered Locus Names:	YGR002C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Ref.4 Ref.10 Ref.11
Subunit structure	Component of the SWR1 chromatin-remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2. Interacts with YAF9. Ref.7
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 2230 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the SWC4 family. Contains 1 SANT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Chromatin regulator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay. Source: SGD establishment of mitotic sister chromatid cohesion Inferred from mutant phenotype. Source: SGD histone acetylation Inferred from physical interaction. Source: SGD histone exchange Inferred from physical interaction. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	NuA4 histone acetyltransferase complex Inferred from physical interaction Ref.9 Ref.8. Source: SGD Swr1 complex Inferred from direct assay Ref.11 Ref.4. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.11 Ref.10. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
EAF1	Q06337	4	EBI-23061,EBI-35867
ESA1	Q08649	8	EBI-23061,EBI-6648
RVB2	Q12464	3	EBI-23061,EBI-31814
SWR1	Q05471	6	EBI-23061,EBI-22102
TRA1	P38811	4	EBI-23061,EBI-24638
YAF9	P53930	5	EBI-23061,EBI-28841

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 476

476

SWR1-complex protein 4

PRO_0000072345

Regions

Domain

156 – 209

SANT

Sequences

Sequence

Length

Mass (Da)

Tools

P53201 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 512CB8DF3171E7A3
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FASTA

476

55,213

        10         20         30         40         50         60 
MSSSDIFDVL NIKQKSRSPT NGQVSVPSSS AANRPKPQVT GMQRELFNLL GENQPPVVIK 

        70         80         90        100        110        120 
SGNNFKEKML STSKPSPWSF VEFKANNSVT LRHWVKGSKE LIGDTPKESP YSKFNQHLSI 

       130        140        150        160        170        180 
PSFTKEEYEA FMNENEGTQK SVESEKNHNE NFTNEKKDES KNSWSFEEIE YLFNLCKKYD 

       190        200        210        220        230        240 
LRWFLIFDRY SYNNSRTLED LKEKFYYTCR NYFKASDPSN PLLSSLNFSA EKEIERKKYL 

       250        260        270        280        290        300 
QRLLSRSAAE IAEEEALVVE SKKFEMAAKR TLAERESLLR LLDSPHSDQT ITQYLTSQGM 

       310        320        330        340        350        360 
SQLYNALLAD KTRKRKHDLN IPENPWMKQQ QQFAQHRQLQ QLNVKKSEVK ENLSPKKTKR 

       370        380        390        400        410        420 
QRQEMQTALK RKSESAYAEQ LLKDFNSDER KALGVITHGE KLSPGVYLRS TKLSTFKPAL 

       430        440        450        460        470 
QNKILAILQE LSLPSRPVMP SFDVMERQEE LLKKINTLID LKKHVDKYEA GMSITK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed: 14690608] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Direct physical and functional interaction of the NuA4 complex components Yaf9p and Swc4p." Bittner C.B., Zeisig D.T., Zeisig B.B., Slany R.K. Eukaryot. Cell 3:976-983(2004) [PubMed: 15302830] [Abstract] Cited for: INTERACTION WITH YAF9.
[8]	"The Yaf9 component of the SWR1 and NuA4 complexes is required for proper gene expression, histone H4 acetylation, and Htz1 replacement near telomeres." Zhang H., Richardson D.O., Roberts D.N., Utley R.T., Erdjument-Bromage H., Tempst P., Cote J., Cairns B.R. Mol. Cell. Biol. 24:9424-9436(2004) [PubMed: 15485911] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
[9]	"Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4." Krogan N.J., Baetz K., Keogh M.-C., Datta N., Sawa C., Kwok T.C.Y., Thompson N.J., Davey M.G., Pootoolal J., Hughes T.R., Emili A., Buratowski S., Hieter P., Greenblatt J.F. Proc. Natl. Acad. Sci. U.S.A. 101:13513-13518(2004) [PubMed: 15353583] [Abstract] Cited for: IDENTIFICATION IN THE NUA4 COMPLEX, MASS SPECTROMETRY.
[10]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed: 15045029] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, MASS SPECTROMETRY.
[11]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed: 14645854] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72787 Genomic DNA. Translation: CAA96985.1. AY692656 Genomic DNA. Translation: AAT92675.1. BK006941 Genomic DNA. Translation: DAA08100.1.
PIR	S64291.
RefSeq	NP_011516.1. NM_001181131.1.
3D structure databases
ProteinModelPortal	P53201.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6510N.
IntAct	P53201. 33 interactions.
MINT	MINT-675383.
STRING	P53201.
Proteomic databases
PeptideAtlas	P53201.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR002C; YGR002C; YGR002C.
GeneID	852885.
KEGG	sce:YGR002C.
NMPDR	fig\|4932.3.peg.2626.
Organism-specific databases
CYGD	YGR002c.
SGD	S000003234. SWC4.
Phylogenomic databases
eggNOG	fuNOG07494.
GeneTree	EFGT00050000004114.
HOGENOM	HBG676997.
OMA	KFEMAAK.
OrthoDB	EOG4ZGSN8.
Gene expression databases
ArrayExpress	P53201.
Genevestigator	P53201.
GermOnline	YGR002C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR001005. SANT_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K11324.
SMART	SM00717. SANT. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS51293. SANT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	972538.

Entry information

Entry name

SWC4_YEAST

Accession

Primary (citable) accession number: P53201
Secondary accession number(s): D6VUD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents