P53199 (ERG26_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
EC=1.1.1.170

Gene names

Name:	ERG26
Ordered Locus Names:	YGL001C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.
Subunit structure	Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Ref.6 Ref.9.
Miscellaneous	Present with 2580 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the 3-beta-HSD family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Endoplasmic reticulum Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ergosterol biosynthetic process Inferred from direct assay Ref.4. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay Ref.6. Source: SGD
Molecular_function	3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: InterPro C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity Inferred from direct assay Ref.4. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG28	P40030	3	EBI-6514,EBI-22518
MCM7	P38132	2	EBI-6514,EBI-4300

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating		PRO_0000087798
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	155	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53199 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 87CA8FF8D64B0327	FASTA	349	38,706
	10 20 30 40 50 60 MSKIDSVLII GGSGFLGLHL IQQFFDINPK PDIHIFDVRD LPEKLSKQFT FNVDDIKFHK 70 80 90 100 110 120 GDLTSPDDME NAINESKANV VVHCASPMHG QNPDIYDIVN VKGTRNVIDM CKKCGVNILV 130 140 150 160 170 180 YTSSAGVIFN GQDVHNADET WPIPEVPMDA YNETKAIAED MVLKANDPSS DFYTVALRPA 190 200 210 220 230 240 GIFGPGDRQL VPGLRQVAKL GQSKFQIGDN NNLFDWTYAG NVADAHVLAA QKLLDPKTRT 250 260 270 280 290 300 AVSGETFFIT NDTPTYFWAL ARTVWKADGH IDKHVIVLKR PVAICAGYLS EWVSKMLGKE 310 320 330 340 PGLTPFRVKI VCAYRYHNIA KAKKLLGYTP RVGIEEGINK TLAWMDEGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis." Gachotte D., Barbuch R., Gaylor J., Nickel E., Bard M. Proc. Natl. Acad. Sci. U.S.A. 95:13794-13799(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, FUNCTION.
[5]	Erratum Gachotte D., Barbuch R., Gaylor J., Nickel E., Bard M. Proc. Natl. Acad. Sci. U.S.A. 96:1810-1810(1999)
[6]	"Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis." Mo C., Valachovic M., Randall S.K., Nickels J.T., Bard M. Proc. Natl. Acad. Sci. U.S.A. 99:9739-9744(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex." Mo C., Bard M. J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ERG28.
[9]	"The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy." Natter K., Leitner P., Faschinger A., Wolinski H., McCraith S., Fields S., Kohlwein S.D. Mol. Cell. Proteomics 4:662-672(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72523 Genomic DNA. Translation: CAA96701.1. AY693026 Genomic DNA. Translation: AAT93045.1. BK006941 Genomic DNA. Translation: DAA08098.1.
PIR	S64003.
RefSeq	NP_011514.1. NM_001180866.1.
3D structure databases
ProteinModelPortal	P53199.
SMR	P53199. Positions 2-342.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6786N.
IntAct	P53199. 22 interactions.
MINT	MINT-666123.
STRING	4932.YGL001C.
Proteomic databases
PaxDb	P53199.
PeptideAtlas	P53199.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL001C; YGL001C; YGL001C.
GeneID	852883.
KEGG	sce:YGL001C.
Organism-specific databases
CYGD	YGL001c.
SGD	S000002969. ERG26.
Phylogenomic databases
eggNOG	COG0451.
GeneTree	ENSGT00550000074557.
HOGENOM	HOG000167989.
KO	K07748.
OMA	IQLQPTF.
OrthoDB	EOG4DNJD4.
Enzyme and pathway databases
BioCyc	MetaCyc:YGL001C-MONOMER.
UniPathway	UPA00770; UER00757.
Gene expression databases
Genevestigator	P53199.
GermOnline	YGL001C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002225. 3Beta_OHSteriod_DH/Estase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01073. 3Beta_HSD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972532.

Entry information

Entry name

ERG26_YEAST

Accession

Primary (citable) accession number: P53199
Secondary accession number(s): D6VUD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program