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Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

ERG26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathwayi: zymosterol biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (ERG11)
  2. Delta(14)-sterol reductase (ERG24)
  3. Methylsterol monooxygenase (ERG25)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (ERG26)
  5. 3-keto-steroid reductase (ERG27)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151Proton acceptorBy similarity1
Binding sitei155NADBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:YGL001C-MONOMER
YEAST:YGL001C-MONOMER
ReactomeiR-SCE-191273 Cholesterol biosynthesis
R-SCE-193048 Androgen biosynthesis
R-SCE-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-SCE-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
R-SCE-193807 Synthesis of bile acids and bile salts via 27-hydroxycholesterol
R-SCE-193993 Mineralocorticoid biosynthesis
R-SCE-194002 Glucocorticoid biosynthesis
UniPathwayiUPA00770; UER00757

Chemistry databases

SwissLipidsiSLP:000001310

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
Gene namesi
Name:ERG26
Ordered Locus Names:YGL001C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL001C
SGDiS000002969 ERG26

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877981 – 349Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingAdd BLAST349

Proteomic databases

MaxQBiP53199
PaxDbiP53199
PRIDEiP53199

Interactioni

Subunit structurei

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33244, 270 interactors
DIPiDIP-6786N
IntActiP53199, 23 interactors
MINTiP53199
STRINGi4932.YGL001C

Structurei

3D structure databases

ProteinModelPortaliP53199
SMRiP53199
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074557
HOGENOMiHOG000167989
InParanoidiP53199
KOiK07748
OMAiHYYSCER
OrthoDBiEOG092C3T8J

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits

Sequencei

Sequence statusi: Complete.

P53199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIDSVLII GGSGFLGLHL IQQFFDINPK PDIHIFDVRD LPEKLSKQFT
60 70 80 90 100
FNVDDIKFHK GDLTSPDDME NAINESKANV VVHCASPMHG QNPDIYDIVN
110 120 130 140 150
VKGTRNVIDM CKKCGVNILV YTSSAGVIFN GQDVHNADET WPIPEVPMDA
160 170 180 190 200
YNETKAIAED MVLKANDPSS DFYTVALRPA GIFGPGDRQL VPGLRQVAKL
210 220 230 240 250
GQSKFQIGDN NNLFDWTYAG NVADAHVLAA QKLLDPKTRT AVSGETFFIT
260 270 280 290 300
NDTPTYFWAL ARTVWKADGH IDKHVIVLKR PVAICAGYLS EWVSKMLGKE
310 320 330 340
PGLTPFRVKI VCAYRYHNIA KAKKLLGYTP RVGIEEGINK TLAWMDEGL
Length:349
Mass (Da):38,706
Last modified:October 1, 1996 - v1
Checksum:i87CA8FF8D64B0327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72523 Genomic DNA Translation: CAA96701.1
AY693026 Genomic DNA Translation: AAT93045.1
BK006941 Genomic DNA Translation: DAA08098.1
PIRiS64003
RefSeqiNP_011514.1, NM_001180866.1

Genome annotation databases

EnsemblFungiiYGL001C; YGL001C; YGL001C
GeneIDi852883
KEGGisce:YGL001C

Similar proteinsi

Entry informationi

Entry nameiERG26_YEAST
AccessioniPrimary (citable) accession number: P53199
Secondary accession number(s): D6VUD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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