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P53199

- ERG26_YEAST

UniProt

P53199 - ERG26_YEAST

Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

ERG26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei151 – 1511Proton acceptorBy similarity
    Binding sitei155 – 1551NADBy similarity

    GO - Molecular functioni

    1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: InterPro
    2. C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity Source: SGD
    3. protein binding Source: IntAct
    4. sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity Source: UniProtKB-EC

    GO - Biological processi

    1. ergosterol biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:YGL001C-MONOMER.
    YEAST:YGL001C-MONOMER.
    UniPathwayiUPA00770; UER00757.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
    Gene namesi
    Name:ERG26
    Ordered Locus Names:YGL001C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL001c.
    SGDiS000002969. ERG26.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Peripheral membrane protein 2 Publications

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingPRO_0000087798Add
    BLAST

    Proteomic databases

    MaxQBiP53199.
    PaxDbiP53199.
    PeptideAtlasiP53199.

    Expressioni

    Gene expression databases

    GenevestigatoriP53199.

    Interactioni

    Subunit structurei

    Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ERG28P400303EBI-6514,EBI-22518
    MCM7P381322EBI-6514,EBI-4300

    Protein-protein interaction databases

    BioGridi33244. 121 interactions.
    DIPiDIP-6786N.
    IntActiP53199. 22 interactions.
    MINTiMINT-666123.
    STRINGi4932.YGL001C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53199.
    SMRiP53199. Positions 2-342.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 3-beta-HSD family.Curated

    Phylogenomic databases

    eggNOGiCOG0451.
    GeneTreeiENSGT00550000074557.
    HOGENOMiHOG000167989.
    KOiK07748.
    OMAiEPMRDQV.
    OrthoDBiEOG72G1HV.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53199-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIDSVLII GGSGFLGLHL IQQFFDINPK PDIHIFDVRD LPEKLSKQFT    50
    FNVDDIKFHK GDLTSPDDME NAINESKANV VVHCASPMHG QNPDIYDIVN 100
    VKGTRNVIDM CKKCGVNILV YTSSAGVIFN GQDVHNADET WPIPEVPMDA 150
    YNETKAIAED MVLKANDPSS DFYTVALRPA GIFGPGDRQL VPGLRQVAKL 200
    GQSKFQIGDN NNLFDWTYAG NVADAHVLAA QKLLDPKTRT AVSGETFFIT 250
    NDTPTYFWAL ARTVWKADGH IDKHVIVLKR PVAICAGYLS EWVSKMLGKE 300
    PGLTPFRVKI VCAYRYHNIA KAKKLLGYTP RVGIEEGINK TLAWMDEGL 349
    Length:349
    Mass (Da):38,706
    Last modified:October 1, 1996 - v1
    Checksum:i87CA8FF8D64B0327
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72523 Genomic DNA. Translation: CAA96701.1.
    AY693026 Genomic DNA. Translation: AAT93045.1.
    BK006941 Genomic DNA. Translation: DAA08098.1.
    PIRiS64003.
    RefSeqiNP_011514.1. NM_001180866.1.

    Genome annotation databases

    EnsemblFungiiYGL001C; YGL001C; YGL001C.
    GeneIDi852883.
    KEGGisce:YGL001C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72523 Genomic DNA. Translation: CAA96701.1 .
    AY693026 Genomic DNA. Translation: AAT93045.1 .
    BK006941 Genomic DNA. Translation: DAA08098.1 .
    PIRi S64003.
    RefSeqi NP_011514.1. NM_001180866.1.

    3D structure databases

    ProteinModelPortali P53199.
    SMRi P53199. Positions 2-342.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33244. 121 interactions.
    DIPi DIP-6786N.
    IntActi P53199. 22 interactions.
    MINTi MINT-666123.
    STRINGi 4932.YGL001C.

    Proteomic databases

    MaxQBi P53199.
    PaxDbi P53199.
    PeptideAtlasi P53199.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL001C ; YGL001C ; YGL001C .
    GeneIDi 852883.
    KEGGi sce:YGL001C.

    Organism-specific databases

    CYGDi YGL001c.
    SGDi S000002969. ERG26.

    Phylogenomic databases

    eggNOGi COG0451.
    GeneTreei ENSGT00550000074557.
    HOGENOMi HOG000167989.
    KOi K07748.
    OMAi EPMRDQV.
    OrthoDBi EOG72G1HV.

    Enzyme and pathway databases

    UniPathwayi UPA00770 ; UER00757 .
    BioCyci MetaCyc:YGL001C-MONOMER.
    YEAST:YGL001C-MONOMER.

    Miscellaneous databases

    NextBioi 972532.
    PROi P53199.

    Gene expression databases

    Genevestigatori P53199.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01073. 3Beta_HSD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis."
      Gachotte D., Barbuch R., Gaylor J., Nickel E., Bard M.
      Proc. Natl. Acad. Sci. U.S.A. 95:13794-13799(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, FUNCTION.
    5. Erratum
      Gachotte D., Barbuch R., Gaylor J., Nickel E., Bard M.
      Proc. Natl. Acad. Sci. U.S.A. 96:1810-1810(1999)
    6. "Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis."
      Mo C., Valachovic M., Randall S.K., Nickels J.T., Bard M.
      Proc. Natl. Acad. Sci. U.S.A. 99:9739-9744(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, SUBCELLULAR LOCATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
      Mo C., Bard M.
      J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ERG28.
    9. "The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy."
      Natter K., Leitner P., Faschinger A., Wolinski H., McCraith S., Fields S., Kohlwein S.D.
      Mol. Cell. Proteomics 4:662-672(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiERG26_YEAST
    AccessioniPrimary (citable) accession number: P53199
    Secondary accession number(s): D6VUD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2580 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3