Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P53199

- ERG26_YEAST

UniProt

P53199 - ERG26_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating

Gene

ERG26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A 3-beta-hydroxysteroid-4-alpha-carboxylate + NAD(P)+ = a 3-oxosteroid + CO2 + NAD(P)H.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei151 – 1511Proton acceptorBy similarity
Binding sitei155 – 1551NADBy similarity

GO - Molecular functioni

  1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: InterPro
  2. C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity Source: SGD
  3. sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity Source: UniProtKB-EC

GO - Biological processi

  1. ergosterol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:YGL001C-MONOMER.
YEAST:YGL001C-MONOMER.
UniPathwayiUPA00770; UER00757.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (EC:1.1.1.170)
Gene namesi
Name:ERG26
Ordered Locus Names:YGL001C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL001c.
SGDiS000002969. ERG26.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylatingPRO_0000087798Add
BLAST

Proteomic databases

MaxQBiP53199.
PaxDbiP53199.
PeptideAtlasiP53199.

Expressioni

Gene expression databases

GenevestigatoriP53199.

Interactioni

Subunit structurei

Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ERG28P400303EBI-6514,EBI-22518
MCM7P381322EBI-6514,EBI-4300

Protein-protein interaction databases

BioGridi33244. 122 interactions.
DIPiDIP-6786N.
IntActiP53199. 22 interactions.
MINTiMINT-666123.
STRINGi4932.YGL001C.

Structurei

3D structure databases

ProteinModelPortaliP53199.
SMRiP53199. Positions 2-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
InParanoidiP53199.
KOiK07748.
OMAiEPMRDQV.
OrthoDBiEOG72G1HV.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53199-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKIDSVLII GGSGFLGLHL IQQFFDINPK PDIHIFDVRD LPEKLSKQFT
60 70 80 90 100
FNVDDIKFHK GDLTSPDDME NAINESKANV VVHCASPMHG QNPDIYDIVN
110 120 130 140 150
VKGTRNVIDM CKKCGVNILV YTSSAGVIFN GQDVHNADET WPIPEVPMDA
160 170 180 190 200
YNETKAIAED MVLKANDPSS DFYTVALRPA GIFGPGDRQL VPGLRQVAKL
210 220 230 240 250
GQSKFQIGDN NNLFDWTYAG NVADAHVLAA QKLLDPKTRT AVSGETFFIT
260 270 280 290 300
NDTPTYFWAL ARTVWKADGH IDKHVIVLKR PVAICAGYLS EWVSKMLGKE
310 320 330 340
PGLTPFRVKI VCAYRYHNIA KAKKLLGYTP RVGIEEGINK TLAWMDEGL
Length:349
Mass (Da):38,706
Last modified:October 1, 1996 - v1
Checksum:i87CA8FF8D64B0327
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72523 Genomic DNA. Translation: CAA96701.1.
AY693026 Genomic DNA. Translation: AAT93045.1.
BK006941 Genomic DNA. Translation: DAA08098.1.
PIRiS64003.
RefSeqiNP_011514.1. NM_001180866.1.

Genome annotation databases

EnsemblFungiiYGL001C; YGL001C; YGL001C.
GeneIDi852883.
KEGGisce:YGL001C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72523 Genomic DNA. Translation: CAA96701.1 .
AY693026 Genomic DNA. Translation: AAT93045.1 .
BK006941 Genomic DNA. Translation: DAA08098.1 .
PIRi S64003.
RefSeqi NP_011514.1. NM_001180866.1.

3D structure databases

ProteinModelPortali P53199.
SMRi P53199. Positions 2-342.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33244. 122 interactions.
DIPi DIP-6786N.
IntActi P53199. 22 interactions.
MINTi MINT-666123.
STRINGi 4932.YGL001C.

Proteomic databases

MaxQBi P53199.
PaxDbi P53199.
PeptideAtlasi P53199.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL001C ; YGL001C ; YGL001C .
GeneIDi 852883.
KEGGi sce:YGL001C.

Organism-specific databases

CYGDi YGL001c.
SGDi S000002969. ERG26.

Phylogenomic databases

eggNOGi COG0451.
GeneTreei ENSGT00550000074557.
HOGENOMi HOG000167989.
InParanoidi P53199.
KOi K07748.
OMAi EPMRDQV.
OrthoDBi EOG72G1HV.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00757 .
BioCyci MetaCyc:YGL001C-MONOMER.
YEAST:YGL001C-MONOMER.

Miscellaneous databases

NextBioi 972532.
PROi P53199.

Gene expression databases

Genevestigatori P53199.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01073. 3Beta_HSD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis."
    Gachotte D., Barbuch R., Gaylor J., Nickel E., Bard M.
    Proc. Natl. Acad. Sci. U.S.A. 95:13794-13799(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, FUNCTION.
  5. Erratum
    Gachotte D., Barbuch R., Gaylor J., Nickel E., Bard M.
    Proc. Natl. Acad. Sci. U.S.A. 96:1810-1810(1999)
  6. "Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis."
    Mo C., Valachovic M., Randall S.K., Nickels J.T., Bard M.
    Proc. Natl. Acad. Sci. U.S.A. 99:9739-9744(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex."
    Mo C., Bard M.
    J. Lipid Res. 46:1991-1998(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ERG28.
  9. "The spatial organization of lipid synthesis in the yeast Saccharomyces cerevisiae derived from large scale green fluorescent protein tagging and high resolution microscopy."
    Natter K., Leitner P., Faschinger A., Wolinski H., McCraith S., Fields S., Kohlwein S.D.
    Mol. Cell. Proteomics 4:662-672(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiERG26_YEAST
AccessioniPrimary (citable) accession number: P53199
Secondary accession number(s): D6VUD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3