APC/C activator protein CDH1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P53197 (CDH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
APC/C activator protein CDH1

Alternative name(s):
CDC20 homolog 1
Homolog of CDC twenty 1

Gene names

Name:	CDH1
Synonyms:	HCT1
Ordered Locus Names:	YGL003C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway. Ref.6 Ref.7 Ref.9
Subunit structure	Associates with the APC/C complex. Interacts with CLB2, CLB3, CDC5, HSL1, MSN5 and PSE1. Ref.7 Ref.8 Ref.10
Subcellular location	Cytoplasm. Nucleus. Note: Nuclear import and export are mediated by the importin PSE1 and the exportin MSN5. Ref.10
Domain	The C-box is required for the association with the APC/C complex By similarity. Ref.7
Post-translational modification	Phosphorylated at multiple sites by CDC28, probably in its CLB5 bound form, in S, G2 and M phase of the cell cycle, thereby blocking the association of CDH1 to the APC/C and promoting nuclear export of CDH1 by MSN5. Dephosphorylated and activated by CDC14 in late anaphase, which may be necessary for PSE1-dependent nuclear localization. Ref.4 Ref.5 Ref.11
Sequence similarities	Belongs to the WD repeat CDC20/Fizzy family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of mitotic anaphase-promoting complex activity Inferred from mutant phenotype PubMed 9334304. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of spindle pole body separation Inferred from mutant phenotype PubMed 16688214. Source: SGD positive regulation of cyclin catabolic process Inferred from direct assay PubMed 17178718. Source: SGD positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from direct assay PubMed 18172166. Source: SGD regulation of cell size Inferred from mutant phenotype PubMed 12089449. Source: SGD
Cellular_component	anaphase-promoting complex Inferred from physical interaction Ref.4. Source: SGD cytoplasm Inferred from direct assay Ref.10. Source: SGD
Molecular_function	anaphase-promoting complex binding Inferred from direct assay PubMed 15916961. Source: SGD cyclin binding Inferred from direct assay Ref.7. Source: SGD ubiquitin-protein ligase activator activity Inferred from direct assay PubMed 19362536. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 566

566

APC/C activator protein CDH1

PRO_0000050904

Regions

Repeat

258 – 298

WD 1

Repeat

300 – 339

WD 2

Repeat

342 – 379

WD 3

Repeat

383 – 422

WD 4

Repeat

425 – 467

WD 5

Repeat

469 – 510

WD 6

Repeat

513 – 552

WD 7

Motif

55 – 61

C-box

Compositional bias

32 – 38

Poly-Ser

Amino acid modifications

Modified residue

156

Phosphoserine Ref.13

Modified residue

157

Phosphothreonine Ref.12 Ref.13

Experimental info

Mutagenesis

T → A: Abolishes phosphorylation; when associated with A-16; A-42; A-157; A-169; A-173; A-176; A-227 and A-239. Ref.4

Mutagenesis

S → A: Abolishes phosphorylation; when associated with A-12; A-42; A-157; A-169; A-173; A-176; A-227 and A-239. Ref.4

Mutagenesis

S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-157; A-169; A-173; A-176; A-227 and A-239. Ref.4

Mutagenesis

157

T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-169; A-173; A-176; A-227 and A-239. Ref.4

Mutagenesis

169

S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-173; A-176; A-227 and A-239. Ref.4

Mutagenesis

173

T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-176; A-227 and A-239. Ref.4

Mutagenesis

176

T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-227 and A-239. Ref.4

Mutagenesis

227

S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-239. Ref.4

Mutagenesis

239

S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-227. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P53197 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: DA6DE5545607906B
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FASTA

566

62,821

        10         20         30         40         50         60 
MSTNLNPFMN NTPSSSPLKG SESKRVSKRP ISSSSSASLL SSPSRRSRPS TVYGDRYIPS 

        70         80         90        100        110        120 
RTDIDFNSIV SISSMASVPA LNPSSTEDQV EYQKERQAHE TYNTLLKNEL FGEMLSKDTV 

       130        140        150        160        170        180 
GSESSIDRIK NTRPSTRGNV HAENTTRHGY ELERVSTPPP EAAGLEEFSP HSTPVTPRRL 

       190        200        210        220        230        240 
FTSQQDEITR PSSNSVRGAS LLTYQQRKGR RLSAASLLQS QFFDSMSPVR PDSKQLLLSP 

       250        260        270        280        290        300 
GKQFRQIAKV PYRVLDAPSL ADDFYYSLID WSSTDVLAVA LGKSIFLTDN NTGDVVHLCD 

       310        320        330        340        350        360 
TENEYTSLSW IGAGSHLAVG QANGLVEIYD VMKRKCIRTL SGHIDRVACL SWNNHVLTSG 

       370        380        390        400        410        420 
SRDHRILHRD VRMPDPFFET IESHTQEVCG LKWNVADNKL ASGGNDNVVH VYEGTSKSPI 

       430        440        450        460        470        480 
LTFDEHKAAV KAMAWSPHKR GVLATGGGTA DRRLKIWNVN TSIKMSDIDS GSQICNMVWS 

       490        500        510        520        530        540 
KNTNELVTSH GYSKYNLTLW DCNSMDPIAI LKGHSFRVLH LTLSNDGTTV VSGAGDETLR 

       550        560 
YWKLFDKPKA KVQPNSLIFD AFNQIR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex." Zachariae W., Schwab M., Nasmyth K., Seufert W. Science 282:1721-1724(1998) [PubMed] [Europe PMC] [Abstract] Cited for: ASSOCIATION WITH THE APC/C COMPLEX, PHOSPHORYLATION BY CDC28, MUTAGENESIS OF THR-12; SER-16; SER-42; THR-157; SER-169; THR-173; THR-176; SER-227 AND SER-239.
[5]	"Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14." Jaspersen S.L., Charles J.F., Morgan D.O. Curr. Biol. 9:227-236(1999) [PubMed] [Europe PMC] [Abstract] Cited for: DEPHOSPHORYLATION BY CDC14.
[6]	"Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage." Alexandru G., Zachariae W., Schleiffer A., Nasmyth K. EMBO J. 18:2707-2721(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS SPINDLE CHECKPOINT TARGET.
[7]	"Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC." Schwab M., Neutzner M., Moecker D., Seufert W. EMBO J. 20:5165-5175(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CLB2; CLB3 AND CDC5, DOMAIN C-BOX MOTIF.
[8]	"D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p." Burton J.L., Solomon M.J. Genes Dev. 15:2381-2395(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HSL1.
[9]	"Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p." Huang J.N., Park I., Ellingson E., Littlepage L.E., Pellman D. J. Cell Biol. 154:85-94(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ASE1 AND CDC20 DEGRADATION.
[10]	"Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1)." Jaquenoud M., van Drogen F., Peter M. EMBO J. 21:6515-6526(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MSN5 AND PSE1.
[11]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-157, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156 AND THR-157, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z72525 Genomic DNA. Translation: CAA96703.1. AY693078 Genomic DNA. Translation: AAT93097.1. BK006941 Genomic DNA. Translation: DAA08096.1.
PIR	S64005.
RefSeq	NP_011512.1. NM_001180868.1.
3D structure databases
ProteinModelPortal	P53197.
SMR	P53197. Positions 245-546.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5915N.
IntAct	P53197. 7 interactions.
MINT	MINT-673168.
STRING	4932.YGL003C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL003C; YGL003C; YGL003C.
GeneID	852881.
KEGG	sce:YGL003C.
Organism-specific databases
CYGD	YGL003c.
SGD	S000002971. CDH1.
Phylogenomic databases
eggNOG	COG2319.
GeneTree	ENSGT00580000081606.
HOGENOM	HOG000195514.
KO	K03364.
OMA	TSHGYSK.
OrthoDB	EOG43FM5B.
Enzyme and pathway databases
BioCyc	YEAST:G3O-30527-MONOMER.
Gene expression databases
Genevestigator	P53197.
GermOnline	YGL003C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF00400. WD40. 4 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972526.

Entry information

Entry name

CDH1_YEAST

Accession

Primary (citable) accession number: P53197
Secondary accession number(s): D6VUD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents