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P53197

- CDH1_YEAST

UniProt

P53197 - CDH1_YEAST

Protein

APC/C activator protein CDH1

Gene

CDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway.3 Publications

    GO - Molecular functioni

    1. anaphase-promoting complex binding Source: SGD
    2. cyclin binding Source: SGD
    3. protein binding Source: IntAct
    4. ubiquitin-protein transferase activator activity Source: SGD

    GO - Biological processi

    1. activation of mitotic anaphase-promoting complex activity Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. negative regulation of spindle pole body separation Source: SGD
    4. positive regulation of cyclin catabolic process Source: SGD
    5. positive regulation of mitotic metaphase/anaphase transition Source: SGD
    6. positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30527-MONOMER.
    ReactomeiREACT_188466. Regulation of APC/C activators between G1/S and early anaphase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    APC/C activator protein CDH1
    Alternative name(s):
    CDC20 homolog 1
    Homolog of CDC twenty 1
    Gene namesi
    Name:CDH1
    Synonyms:HCT1
    Ordered Locus Names:YGL003C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL003c.
    SGDiS000002971. CDH1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Nuclear import and export are mediated by the importin PSE1 and the exportin MSN5.

    GO - Cellular componenti

    1. anaphase-promoting complex Source: SGD
    2. cytoplasm Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi12 – 121T → A: Abolishes phosphorylation; when associated with A-16; A-42; A-157; A-169; A-173; A-176; A-227 and A-239. 1 Publication
    Mutagenesisi16 – 161S → A: Abolishes phosphorylation; when associated with A-12; A-42; A-157; A-169; A-173; A-176; A-227 and A-239. 1 Publication
    Mutagenesisi42 – 421S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-157; A-169; A-173; A-176; A-227 and A-239. 1 Publication
    Mutagenesisi157 – 1571T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-169; A-173; A-176; A-227 and A-239. 1 Publication
    Mutagenesisi169 – 1691S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-173; A-176; A-227 and A-239. 1 Publication
    Mutagenesisi173 – 1731T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-176; A-227 and A-239. 1 Publication
    Mutagenesisi176 – 1761T → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-227 and A-239. 1 Publication
    Mutagenesisi227 – 2271S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-239. 1 Publication
    Mutagenesisi239 – 2391S → A: Abolishes phosphorylation; when associated with A-12; A-16; A-42; A-157; A-169; A-173; A-176 and A-227. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566APC/C activator protein CDH1PRO_0000050904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei213 – 2131Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated at multiple sites by CDC28, probably in its CLB5 bound form, in S, G2 and M phase of the cell cycle, thereby blocking the association of CDH1 to the APC/C and promoting nuclear export of CDH1 by MSN5. Dephosphorylated and activated by CDC14 in late anaphase, which may be necessary for PSE1-dependent nuclear localization.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53197.

    Expressioni

    Gene expression databases

    GenevestigatoriP53197.

    Interactioni

    Subunit structurei

    Associates with the APC/C complex. Interacts with CLB2, CLB3, CDC5, HSL1, MSN5 and PSE1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ACM1Q089818EBI-23684,EBI-2345174

    Protein-protein interaction databases

    BioGridi33242. 275 interactions.
    DIPiDIP-5915N.
    IntActiP53197. 4 interactions.
    MINTiMINT-673168.
    STRINGi4932.YGL003C.

    Structurei

    Secondary structure

    1
    566
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi252 – 2565
    Beta strandi269 – 2713
    Beta strandi275 – 2817
    Beta strandi284 – 2896
    Turni290 – 2923
    Beta strandi295 – 3006
    Beta strandi305 – 3106
    Beta strandi314 – 3218
    Beta strandi326 – 3305
    Turni331 – 3344
    Beta strandi335 – 3406
    Beta strandi347 – 3537
    Beta strandi356 – 3649
    Beta strandi366 – 3705
    Beta strandi373 – 3764
    Beta strandi378 – 3814
    Beta strandi388 – 3936
    Beta strandi395 – 3973
    Beta strandi399 – 4046
    Beta strandi409 – 4135
    Beta strandi420 – 4234
    Beta strandi430 – 4356
    Beta strandi437 – 4393
    Beta strandi442 – 4476
    Turni449 – 4513
    Beta strandi453 – 4586
    Turni459 – 4624
    Beta strandi463 – 4697
    Beta strandi474 – 4796
    Beta strandi481 – 4844
    Beta strandi486 – 4905
    Beta strandi497 – 5004
    Beta strandi502 – 5043
    Beta strandi507 – 5115
    Beta strandi518 – 5236
    Beta strandi527 – 5348
    Turni535 – 5373
    Beta strandi538 – 5436

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BH6X-ray2.90A/B/C/D/E/F/G/H241-548[»]
    ProteinModelPortaliP53197.
    SMRiP53197. Positions 244-548.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati258 – 29841WD 1Add
    BLAST
    Repeati300 – 33940WD 2Add
    BLAST
    Repeati342 – 37938WD 3Add
    BLAST
    Repeati383 – 42240WD 4Add
    BLAST
    Repeati425 – 46743WD 5Add
    BLAST
    Repeati469 – 51042WD 6Add
    BLAST
    Repeati513 – 55240WD 7Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi55 – 617C-box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi32 – 387Poly-Ser

    Domaini

    The C-box is required for the association with the APC/C complex.By similarity

    Sequence similaritiesi

    Belongs to the WD repeat CDC20/Fizzy family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00750000117562.
    HOGENOMiHOG000195514.
    KOiK03364.
    OMAiLKWNVEE.
    OrthoDBiEOG7MSMZF.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 4 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 3 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53197-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTNLNPFMN NTPSSSPLKG SESKRVSKRP ISSSSSASLL SSPSRRSRPS    50
    TVYGDRYIPS RTDIDFNSIV SISSMASVPA LNPSSTEDQV EYQKERQAHE 100
    TYNTLLKNEL FGEMLSKDTV GSESSIDRIK NTRPSTRGNV HAENTTRHGY 150
    ELERVSTPPP EAAGLEEFSP HSTPVTPRRL FTSQQDEITR PSSNSVRGAS 200
    LLTYQQRKGR RLSAASLLQS QFFDSMSPVR PDSKQLLLSP GKQFRQIAKV 250
    PYRVLDAPSL ADDFYYSLID WSSTDVLAVA LGKSIFLTDN NTGDVVHLCD 300
    TENEYTSLSW IGAGSHLAVG QANGLVEIYD VMKRKCIRTL SGHIDRVACL 350
    SWNNHVLTSG SRDHRILHRD VRMPDPFFET IESHTQEVCG LKWNVADNKL 400
    ASGGNDNVVH VYEGTSKSPI LTFDEHKAAV KAMAWSPHKR GVLATGGGTA 450
    DRRLKIWNVN TSIKMSDIDS GSQICNMVWS KNTNELVTSH GYSKYNLTLW 500
    DCNSMDPIAI LKGHSFRVLH LTLSNDGTTV VSGAGDETLR YWKLFDKPKA 550
    KVQPNSLIFD AFNQIR 566
    Length:566
    Mass (Da):62,821
    Last modified:October 1, 1996 - v1
    Checksum:iDA6DE5545607906B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72525 Genomic DNA. Translation: CAA96703.1.
    AY693078 Genomic DNA. Translation: AAT93097.1.
    BK006941 Genomic DNA. Translation: DAA08096.1.
    PIRiS64005.
    RefSeqiNP_011512.1. NM_001180868.1.

    Genome annotation databases

    EnsemblFungiiYGL003C; YGL003C; YGL003C.
    GeneIDi852881.
    KEGGisce:YGL003C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z72525 Genomic DNA. Translation: CAA96703.1 .
    AY693078 Genomic DNA. Translation: AAT93097.1 .
    BK006941 Genomic DNA. Translation: DAA08096.1 .
    PIRi S64005.
    RefSeqi NP_011512.1. NM_001180868.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BH6 X-ray 2.90 A/B/C/D/E/F/G/H 241-548 [» ]
    ProteinModelPortali P53197.
    SMRi P53197. Positions 244-548.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33242. 275 interactions.
    DIPi DIP-5915N.
    IntActi P53197. 4 interactions.
    MINTi MINT-673168.
    STRINGi 4932.YGL003C.

    Proteomic databases

    MaxQBi P53197.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL003C ; YGL003C ; YGL003C .
    GeneIDi 852881.
    KEGGi sce:YGL003C.

    Organism-specific databases

    CYGDi YGL003c.
    SGDi S000002971. CDH1.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00750000117562.
    HOGENOMi HOG000195514.
    KOi K03364.
    OMAi LKWNVEE.
    OrthoDBi EOG7MSMZF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30527-MONOMER.
    Reactomei REACT_188466. Regulation of APC/C activators between G1/S and early anaphase.

    Miscellaneous databases

    NextBioi 972526.
    PROi P53197.

    Gene expression databases

    Genevestigatori P53197.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 4 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 3 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex."
      Zachariae W., Schwab M., Nasmyth K., Seufert W.
      Science 282:1721-1724(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE APC/C COMPLEX, PHOSPHORYLATION BY CDC28, MUTAGENESIS OF THR-12; SER-16; SER-42; THR-157; SER-169; THR-173; THR-176; SER-227 AND SER-239.
    5. "Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14."
      Jaspersen S.L., Charles J.F., Morgan D.O.
      Curr. Biol. 9:227-236(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEPHOSPHORYLATION BY CDC14.
    6. "Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage."
      Alexandru G., Zachariae W., Schleiffer A., Nasmyth K.
      EMBO J. 18:2707-2721(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS SPINDLE CHECKPOINT TARGET.
    7. "Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC."
      Schwab M., Neutzner M., Moecker D., Seufert W.
      EMBO J. 20:5165-5175(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CLB2; CLB3 AND CDC5, DOMAIN C-BOX MOTIF.
    8. "D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p."
      Burton J.L., Solomon M.J.
      Genes Dev. 15:2381-2395(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HSL1.
    9. "Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p."
      Huang J.N., Park I., Ellingson E., Littlepage L.E., Pellman D.
      J. Cell Biol. 154:85-94(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ASE1 AND CDC20 DEGRADATION.
    10. "Cell cycle-dependent nuclear export of Cdh1p may contribute to the inactivation of APC/C(Cdh1)."
      Jaquenoud M., van Drogen F., Peter M.
      EMBO J. 21:6515-6526(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MSN5 AND PSE1.
    11. Cited for: PHOSPHORYLATION BY CDC28.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDH1_YEAST
    AccessioniPrimary (citable) accession number: P53197
    Secondary accession number(s): D6VUD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3