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P53196

- RPN14_YEAST

UniProt

P53196 - RPN14_YEAST

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Protein
26S proteasome regulatory subunit RPN14
Gene
RPN14, YGL004C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Is not a genuine component of the 26S proteasome, but an auxiliary factor that interacts with the proteasomal ATPase of 19S regulatory particle (RP). Acts as a chaperone which regulates the highly structured assembly of the 19S regulatory particle. Involved in the substrate specificity of the 26S proteasome and is especially involved in the degradation of ubiquitinated GCN4. May contribute to the stability of the 26S proteasome in some stress conditions.4 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. proteasome regulatory particle assembly Source: SGD
  2. ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-30528-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN14
Alternative name(s):
Proteasome non-ATPase subunit 14
Gene namesi
Name:RPN14
Ordered Locus Names:YGL004C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL004c.
SGDiS000002972. RPN14.

Subcellular locationi

Cytoplasm. Nucleus 2 Publications

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleus Source: SGD
  3. proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 41741726S proteasome regulatory subunit RPN14
PRO_0000051475Add
BLAST

Proteomic databases

MaxQBiP53196.
PaxDbiP53196.
PeptideAtlasiP53196.

Expressioni

Gene expression databases

GenevestigatoriP53196.

Interactioni

Subunit structurei

Associates with the 19S proteasome regulatory particle (RP). Interacts directly with RPT5 and RPT6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSM3P383486EBI-23691,EBI-21152
NAS6P500864EBI-23691,EBI-14028
RPT5P332974EBI-23691,EBI-13920
RPT6Q019396EBI-23691,EBI-13914

Protein-protein interaction databases

BioGridi33241. 30 interactions.
DIPiDIP-6617N.
IntActiP53196. 23 interactions.
MINTiMINT-675986.
STRINGi4932.YGL004C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Beta strandi8 – 103
Helixi14 – 196
Beta strandi27 – 359
Beta strandi38 – 458
Turni49 – 513
Beta strandi60 – 656
Beta strandi68 – 736
Beta strandi76 – 816
Beta strandi84 – 863
Turni87 – 904
Beta strandi96 – 1027
Beta strandi104 – 1063
Beta strandi108 – 1136
Beta strandi118 – 1214
Beta strandi127 – 1315
Beta strandi134 – 1374
Beta strandi139 – 1446
Beta strandi148 – 1558
Beta strandi158 – 1647
Turni165 – 1673
Beta strandi172 – 1754
Beta strandi181 – 1877
Turni188 – 1914
Beta strandi192 – 1976
Beta strandi202 – 2065
Turni207 – 2104
Beta strandi211 – 2166
Beta strandi226 – 2338
Helixi240 – 2423
Beta strandi257 – 2637
Beta strandi268 – 2725
Turni273 – 2753
Beta strandi278 – 2825
Beta strandi290 – 2956
Beta strandi302 – 3076
Beta strandi310 – 3167
Beta strandi324 – 3307
Beta strandi335 – 3417
Beta strandi344 – 3496
Turni350 – 3523
Beta strandi353 – 3608
Beta strandi366 – 3705
Beta strandi376 – 3783
Beta strandi387 – 3915
Beta strandi394 – 3974
Beta strandi399 – 4046
Turni405 – 4073
Beta strandi408 – 4147

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ACPX-ray2.00A1-417[»]
3VL1X-ray1.60A/B1-417[»]
ProteinModelPortaliP53196.
SMRiP53196. Positions 1-417.

Miscellaneous databases

EvolutionaryTraceiP53196.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati134 – 17340WD 1
Add
BLAST
Repeati176 – 21540WD 2
Add
BLAST
Repeati242 – 28140WD 3
Add
BLAST
Repeati285 – 32541WD 4
Add
BLAST
Repeati330 – 37142WD 5
Add
BLAST
Repeati380 – 41637WD 6
Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00390000005948.
HOGENOMiHOG000142386.
KOiK11887.
OMAiDESNGEV.
OrthoDBiEOG7TBCBC.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53196-1 [UniParc]FASTAAdd to Basket

« Hide

MTKTITVAHI QYDFKAVLEE NDENDDEFYI NVDKNLNEIK EHKIVVLGNS    50
RGVDAGKGNT FEKVGSHLYK ARLDGHDFLF NTIIRDGSKM LKRADYTAVD 100
TAKLQMRRFI LGTTEGDIKV LDSNFNLQRE IDQAHVSEIT KLKFFPSGEA 150
LISSSQDMQL KIWSVKDGSN PRTLIGHRAT VTDIAIIDRG RNVLSASLDG 200
TIRLWECGTG TTIHTFNRKE NPHDGVNSIA LFVGTDRQLH EISTSKKNNL 250
EFGTYGKYVI AGHVSGVITV HNVFSKEQTI QLPSKFTCSC NSLTVDGNNA 300
NYIYAGYENG MLAQWDLRSP ECPVGEFLIN EGTPINNVYF AAGALFVSSG 350
FDTSIKLDII SDPESERPAI EFETPTFLVS NDDEVSQFCY VSDDESNGEV 400
LEVGKNNFCA LYNLSNP 417
Length:417
Mass (Da):46,383
Last modified:October 1, 1996 - v1
Checksum:i025CAB9A872E1AB8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72526 Genomic DNA. Translation: CAA96704.1.
AY692599 Genomic DNA. Translation: AAT92618.1.
BK006941 Genomic DNA. Translation: DAA08095.1.
PIRiS64006.
RefSeqiNP_011511.3. NM_001180869.3.

Genome annotation databases

EnsemblFungiiYGL004C; YGL004C; YGL004C.
GeneIDi852880.
KEGGisce:YGL004C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z72526 Genomic DNA. Translation: CAA96704.1 .
AY692599 Genomic DNA. Translation: AAT92618.1 .
BK006941 Genomic DNA. Translation: DAA08095.1 .
PIRi S64006.
RefSeqi NP_011511.3. NM_001180869.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ACP X-ray 2.00 A 1-417 [» ]
3VL1 X-ray 1.60 A/B 1-417 [» ]
ProteinModelPortali P53196.
SMRi P53196. Positions 1-417.
ModBasei Search...

Protein-protein interaction databases

BioGridi 33241. 30 interactions.
DIPi DIP-6617N.
IntActi P53196. 23 interactions.
MINTi MINT-675986.
STRINGi 4932.YGL004C.

Proteomic databases

MaxQBi P53196.
PaxDbi P53196.
PeptideAtlasi P53196.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL004C ; YGL004C ; YGL004C .
GeneIDi 852880.
KEGGi sce:YGL004C.

Organism-specific databases

CYGDi YGL004c.
SGDi S000002972. RPN14.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00390000005948.
HOGENOMi HOG000142386.
KOi K11887.
OMAi DESNGEV.
OrthoDBi EOG7TBCBC.

Enzyme and pathway databases

BioCyci YEAST:G3O-30528-MONOMER.

Miscellaneous databases

EvolutionaryTracei P53196.
NextBioi 972523.

Gene expression databases

Genevestigatori P53196.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 2 hits.
[Graphical view ]
SMARTi SM00320. WD40. 4 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome."
    Seong K.M., Baek J.H., Yu M.H., Kim J.
    FEBS Lett. 581:2567-2573(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RPT5.
  7. "Chaperone-mediated pathway of proteasome regulatory particle assembly."
    Roelofs J., Park S., Haas W., Tian G., McAllister F.E., Huo Y., Lee B.H., Zhang F., Shi Y., Gygi S.P., Finley D.
    Nature 459:861-865(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE PROTEASOME REGULATORY PARTICLE.
  8. "Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base."
    Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M.
    Cell 137:887-899(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE 19S REGULATORY PARTICLE OF THE PROTEASOME, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle."
    Saeki Y., Toh-E A., Kudo T., Kawamura H., Tanaka K.
    Cell 137:900-913(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH THE 19S REGULATORY PARTICLE OF THE PROTEASOME, INTERACTION WITH RPT6.

Entry informationi

Entry nameiRPN14_YEAST
AccessioniPrimary (citable) accession number: P53196
Secondary accession number(s): D6VUD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2210 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

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