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P53196 (RPN14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
26S proteasome regulatory subunit RPN14
Alternative name(s):
Proteasome non-ATPase subunit 14
Gene names
Name:RPN14
Ordered Locus Names:YGL004C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Is not a genuine component of the 26S proteasome, but an auxiliary factor that interacts with the proteasomal ATPase of 19S regulatory particle (RP). Acts as a chaperone which regulates the highly structured assembly of the 19S regulatory particle. Involved in the substrate specificity of the 26S proteasome and is especially involved in the degradation of ubiquitinated GCN4. May contribute to the stability of the 26S proteasome in some stress conditions. Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure

Associates with the 19S proteasome regulatory particle (RP). Interacts directly with RPT5 and RPT6. Ref.6 Ref.9

Subcellular location

Cytoplasm. Nucleus Ref.4 Ref.9.

Miscellaneous

Present with 2210 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat PAAF1/RPN14 family.

Contains 6 WD repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSM3P383487EBI-23691,EBI-21152
NAS6P500865EBI-23691,EBI-14028
RPT6Q019396EBI-23691,EBI-13914

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 41741726S proteasome regulatory subunit RPN14
PRO_0000051475

Regions

Repeat134 – 17340WD 1
Repeat176 – 21540WD 2
Repeat242 – 28140WD 3
Repeat285 – 32541WD 4
Repeat330 – 37142WD 5
Repeat380 – 41637WD 6

Secondary structure

1 ........................................................................................ 417
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53196 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 025CAB9A872E1AB8

FASTA41746,383
        10         20         30         40         50         60 
MTKTITVAHI QYDFKAVLEE NDENDDEFYI NVDKNLNEIK EHKIVVLGNS RGVDAGKGNT 

        70         80         90        100        110        120 
FEKVGSHLYK ARLDGHDFLF NTIIRDGSKM LKRADYTAVD TAKLQMRRFI LGTTEGDIKV 

       130        140        150        160        170        180 
LDSNFNLQRE IDQAHVSEIT KLKFFPSGEA LISSSQDMQL KIWSVKDGSN PRTLIGHRAT 

       190        200        210        220        230        240 
VTDIAIIDRG RNVLSASLDG TIRLWECGTG TTIHTFNRKE NPHDGVNSIA LFVGTDRQLH 

       250        260        270        280        290        300 
EISTSKKNNL EFGTYGKYVI AGHVSGVITV HNVFSKEQTI QLPSKFTCSC NSLTVDGNNA 

       310        320        330        340        350        360 
NYIYAGYENG MLAQWDLRSP ECPVGEFLIN EGTPINNVYF AAGALFVSSG FDTSIKLDII 

       370        380        390        400        410 
SDPESERPAI EFETPTFLVS NDDEVSQFCY VSDDESNGEV LEVGKNNFCA LYNLSNP

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome."
Seong K.M., Baek J.H., Yu M.H., Kim J.
FEBS Lett. 581:2567-2573(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RPT5.
[7]"Chaperone-mediated pathway of proteasome regulatory particle assembly."
Roelofs J., Park S., Haas W., Tian G., McAllister F.E., Huo Y., Lee B.H., Zhang F., Shi Y., Gygi S.P., Finley D.
Nature 459:861-865(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE PROTEASOME REGULATORY PARTICLE.
[8]"Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base."
Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M.
Cell 137:887-899(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE 19S REGULATORY PARTICLE OF THE PROTEASOME, MASS SPECTROMETRY.
[9]"Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle."
Saeki Y., Toh-E A., Kudo T., Kawamura H., Tanaka K.
Cell 137:900-913(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH THE 19S REGULATORY PARTICLE OF THE PROTEASOME, INTERACTION WITH RPT6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z72526 Genomic DNA. Translation: CAA96704.1.
AY692599 Genomic DNA. Translation: AAT92618.1.
BK006941 Genomic DNA. Translation: DAA08095.1.
PIRS64006.
RefSeqNP_011511.3. NM_001180869.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ACPX-ray2.00A1-417[»]
3VL1X-ray1.60A/B1-417[»]
ProteinModelPortalP53196.
SMRP53196. Positions 1-417.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6617N.
IntActP53196. 24 interactions.
MINTMINT-675986.
STRING4932.YGL004C.

Proteomic databases

PaxDbP53196.
PeptideAtlasP53196.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL004C; YGL004C; YGL004C.
GeneID852880.
KEGGsce:YGL004C.
sce:YGR001C.

Organism-specific databases

CYGDYGL004c.
SGDS000002972. RPN14.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00390000005948.
HOGENOMHOG000142386.
KOK11887.
OMAIYAGYEN.
OrthoDBEOG4643NR.

Gene expression databases

GenevestigatorP53196.
GermOnlineYGL004C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP53196.
NextBio972523.

Entry information

Entry nameRPN14_YEAST
AccessionPrimary (citable) accession number: P53196
Secondary accession number(s): D6VUD4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents