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Protein

J-type co-chaperone JAC1, mitochondrial

Gene

JAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Co-chaperone required for the assembly of iron-sulfur (Fe/S) clusters in mitochondria. Stimulates the ATPase activity of its specialized Hsp70 chaperone partner SSQ1. Binds to the substrate protein ISU1 and targets it to SSQ1. May function together with SSQ1 in the dislocation of the Fe/S cluster from ISU1 and its insertion into apoproteins.9 Publications

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ATPase activator activity Source: InterPro
  • chaperone binding Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • protein maturation by iron-sulfur cluster transfer Source: InterPro
  • protein oligomerization Source: InterPro
  • protein targeting to mitochondrion Source: Reactome

Keywordsi

Molecular functionChaperone
Biological processStress response

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30538-MONOMER.
YEAST:G3O-30538-MONOMER.
ReactomeiR-SCE-1362409. Mitochondrial iron-sulfur cluster biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
J-type co-chaperone JAC1, mitochondrial
Alternative name(s):
J-type accessory chaperone 1
Gene namesi
Name:JAC1
Synonyms:SEO2
Ordered Locus Names:YGL018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL018C.
SGDiS000002986. JAC1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32Missing in JAC1-1; temperature sensitive; causes growth defect at high temperatures. 1 Publication1
Mutagenesisi48 – 50HPD → AAA in JAC1-A3; temperature sensitive; causes growth defect at high temperatures. 1 Publication3
Mutagenesisi104 – 117LLLKV…DELSQ → ALLAVLAIHAALSA in JAC1(LKDDEQ); impairs interaction with ISU1, but does not affect stimulation of SSQ1 ATPase activity. 1 PublicationAdd BLAST14

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 10Mitochondrion1 Publication10
ChainiPRO_000007116311 – 184J-type co-chaperone JAC1, mitochondrialAdd BLAST174

Proteomic databases

MaxQBiP53193.
PRIDEiP53193.

Interactioni

Subunit structurei

Interacts with the Fe/S cluster assembly protein ISU1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ISU1Q030203EBI-23714,EBI-29901

GO - Molecular functioni

  • chaperone binding Source: SGD

Protein-protein interaction databases

BioGridi33228. 31 interactors.
DIPiDIP-5603N.
IntActiP53193. 1 interactor.
MINTiMINT-567109.
STRINGi4932.YGL018C.

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 17Combined sources3
Turni19 – 21Combined sources3
Helixi33 – 45Combined sources13
Helixi58 – 60Combined sources3
Helixi62 – 70Combined sources9
Helixi72 – 84Combined sources13
Helixi91 – 99Combined sources9
Helixi102 – 117Combined sources16
Helixi121 – 147Combined sources27
Helixi151 – 172Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UO2X-ray2.13A/B10-184[»]
3UO3X-ray1.85A/B5-182[»]
ProteinModelPortaliP53193.
SMRiP53193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 82JPROSITE-ProRule annotationAdd BLAST70

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 184Interaction with ISU1Add BLAST114

Sequence similaritiesi

Belongs to the HscB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000008206.
HOGENOMiHOG000093537.
InParanoidiP53193.
KOiK04082.
OMAiLRYWVNI.
OrthoDBiEOG092C4Z2R.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
1.20.1280.20. 1 hit.
InterProiView protein in InterPro
IPR036869. DnaJ_dom_sf.
IPR001623. DnaJ_domain.
IPR004640. HscB.
IPR036386. HscB_C_sf.
IPR009073. HscB_oligo_C.
PANTHERiPTHR14021. PTHR14021. 1 hit.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF07743. HSCB_C. 1 hit.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF47144. SSF47144. 1 hit.
TIGRFAMsiTIGR00714. hscB. 1 hit.
PROSITEiView protein in PROSITE
PS50076. DNAJ_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKYLVQRRF TSTFYELFPK TFPKKLPIWT IDQSRLRKEY RQLQAQHHPD
60 70 80 90 100
MAQQGSEQSS TLNQAYHTLK DPLRRSQYML KLLRNIDLTQ EQTSNEVTTS
110 120 130 140 150
DPQLLLKVLD IHDELSQMDD EAGVKLLEKQ NKERIQDIEA QLGQCYNDKD
160 170 180
YAAAVKLTVE LKYWYNLAKA FKDWAPGKQL EMNH
Length:184
Mass (Da):21,778
Last modified:October 1, 1996 - v1
Checksum:i92B600F279866DF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72540 Genomic DNA. Translation: CAA96718.1.
AY558479 Genomic DNA. Translation: AAS56805.1.
BK006941 Genomic DNA. Translation: DAA08080.1.
PIRiS64020.
RefSeqiNP_011497.1. NM_001180883.1.

Genome annotation databases

EnsemblFungiiYGL018C; YGL018C; YGL018C.
GeneIDi852866.
KEGGisce:YGL018C.

Similar proteinsi

Entry informationi

Entry nameiJAC1_YEAST
AccessioniPrimary (citable) accession number: P53193
Secondary accession number(s): D6VUB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names