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Protein

J-type co-chaperone JAC1, mitochondrial

Gene

JAC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Co-chaperone required for the assembly of iron-sulfur (Fe/S) clusters in mitochondria. Stimulates the ATPase activity of its specialized Hsp70 chaperone partner SSQ1. Binds to the substrate protein ISU1 and targets it to SSQ1. May function together with SSQ1 in the dislocation of the Fe/S cluster from ISU1 and its insertion into apoproteins.9 Publications

GO - Molecular functioni

  • chaperone binding Source: SGD

GO - Biological processi

  • aerobic respiration Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • protein folding Source: InterPro
  • protein oligomerization Source: InterPro
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30538-MONOMER.
YEAST:G3O-30538-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
J-type co-chaperone JAC1, mitochondrial
Alternative name(s):
J-type accessory chaperone 1
Gene namesi
Name:JAC1
Synonyms:SEO2
Ordered Locus Names:YGL018C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL018C.
SGDiS000002986. JAC1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321Missing in JAC1-1; temperature sensitive; causes growth defect at high temperatures. 1 Publication
Mutagenesisi48 – 503HPD → AAA in JAC1-A3; temperature sensitive; causes growth defect at high temperatures. 1 Publication
Mutagenesisi104 – 11714LLLKV…DELSQ → ALLAVLAIHAALSA in JAC1(LKDDEQ); impairs interaction with ISU1, but does not affect stimulation of SSQ1 ATPase activity. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1010Mitochondrion1 Publication
Chaini11 – 184174J-type co-chaperone JAC1, mitochondrialPRO_0000071163Add
BLAST

Proteomic databases

MaxQBiP53193.

Interactioni

Subunit structurei

Interacts with the Fe/S cluster assembly protein ISU1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ISU1Q030203EBI-23714,EBI-29901

GO - Molecular functioni

  • chaperone binding Source: SGD

Protein-protein interaction databases

BioGridi33228. 31 interactions.
DIPiDIP-5603N.
IntActiP53193. 1 interaction.
MINTiMINT-567109.

Structurei

Secondary structure

1
184
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 173Combined sources
Turni19 – 213Combined sources
Helixi33 – 4513Combined sources
Helixi58 – 603Combined sources
Helixi62 – 709Combined sources
Helixi72 – 8413Combined sources
Helixi91 – 999Combined sources
Helixi102 – 11716Combined sources
Helixi121 – 14727Combined sources
Helixi151 – 17222Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UO2X-ray2.13A/B10-184[»]
3UO3X-ray1.85A/B5-182[»]
ProteinModelPortaliP53193.
SMRiP53193. Positions 8-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 8270JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 184114Interaction with ISU1Add
BLAST

Sequence similaritiesi

Belongs to the HscB family.Curated
Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000008206.
HOGENOMiHOG000093537.
InParanoidiP53193.
KOiK04082.
OMAiNENYEDA.
OrthoDBiEOG092C4Z2R.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
1.20.1280.20. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR004640. HscB.
IPR009073. HscB_oligo_C.
[Graphical view]
PANTHERiPTHR14021. PTHR14021. 1 hit.
PfamiPF00226. DnaJ. 1 hit.
PF07743. HSCB_C. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF47144. SSF47144. 1 hit.
TIGRFAMsiTIGR00714. hscB. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKYLVQRRF TSTFYELFPK TFPKKLPIWT IDQSRLRKEY RQLQAQHHPD
60 70 80 90 100
MAQQGSEQSS TLNQAYHTLK DPLRRSQYML KLLRNIDLTQ EQTSNEVTTS
110 120 130 140 150
DPQLLLKVLD IHDELSQMDD EAGVKLLEKQ NKERIQDIEA QLGQCYNDKD
160 170 180
YAAAVKLTVE LKYWYNLAKA FKDWAPGKQL EMNH
Length:184
Mass (Da):21,778
Last modified:October 1, 1996 - v1
Checksum:i92B600F279866DF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72540 Genomic DNA. Translation: CAA96718.1.
AY558479 Genomic DNA. Translation: AAS56805.1.
BK006941 Genomic DNA. Translation: DAA08080.1.
PIRiS64020.
RefSeqiNP_011497.1. NM_001180883.1.

Genome annotation databases

EnsemblFungiiYGL018C; YGL018C; YGL018C.
GeneIDi852866.
KEGGisce:YGL018C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72540 Genomic DNA. Translation: CAA96718.1.
AY558479 Genomic DNA. Translation: AAS56805.1.
BK006941 Genomic DNA. Translation: DAA08080.1.
PIRiS64020.
RefSeqiNP_011497.1. NM_001180883.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UO2X-ray2.13A/B10-184[»]
3UO3X-ray1.85A/B5-182[»]
ProteinModelPortaliP53193.
SMRiP53193. Positions 8-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33228. 31 interactions.
DIPiDIP-5603N.
IntActiP53193. 1 interaction.
MINTiMINT-567109.

Proteomic databases

MaxQBiP53193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL018C; YGL018C; YGL018C.
GeneIDi852866.
KEGGisce:YGL018C.

Organism-specific databases

EuPathDBiFungiDB:YGL018C.
SGDiS000002986. JAC1.

Phylogenomic databases

GeneTreeiENSGT00390000008206.
HOGENOMiHOG000093537.
InParanoidiP53193.
KOiK04082.
OMAiNENYEDA.
OrthoDBiEOG092C4Z2R.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30538-MONOMER.
YEAST:G3O-30538-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiP53193.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
1.20.1280.20. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR004640. HscB.
IPR009073. HscB_oligo_C.
[Graphical view]
PANTHERiPTHR14021. PTHR14021. 1 hit.
PfamiPF00226. DnaJ. 1 hit.
PF07743. HSCB_C. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF47144. SSF47144. 1 hit.
TIGRFAMsiTIGR00714. hscB. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJAC1_YEAST
AccessioniPrimary (citable) accession number: P53193
Secondary accession number(s): D6VUB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.