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Protein

Golgi to ER traffic protein 1

Gene

GET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.UniRule annotation5 Publications

GO - Molecular functioni

  • protein anchor Source: SGD
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

  • protein insertion into ER membrane Source: SGD
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • tail-anchored membrane protein insertion into ER membrane Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30540-MONOMER.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 1UniRule annotation
Alternative name(s):
Guided entry of tail-anchored proteins 1UniRule annotation
Mitochondrial distribution and morphology protein 39
Gene namesi
Name:GET1UniRule annotation
Synonyms:MDM39
Ordered Locus Names:YGL020C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL020C.
SGDiS000002988. GET1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1LumenalUniRule annotation1
Transmembranei2 – 21HelicalUniRule annotationAdd BLAST20
Topological domaini22 – 104CytoplasmicUniRule annotationAdd BLAST83
Transmembranei105 – 125HelicalUniRule annotationAdd BLAST21
Topological domaini126 – 181LumenalUniRule annotationAdd BLAST56
Transmembranei182 – 198HelicalUniRule annotationAdd BLAST17
Topological domaini199 – 235CytoplasmicUniRule annotationAdd BLAST37

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004148471 – 235Golgi to ER traffic protein 1Add BLAST235

Proteomic databases

MaxQBiP53192.
PRIDEiP53192.

Interactioni

Subunit structurei

Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121543EBI-23722,EBI-2989

GO - Molecular functioni

  • protein anchor Source: SGD

Protein-protein interaction databases

BioGridi33226. 542 interactors.
DIPiDIP-6309N.
IntActiP53192. 3 interactors.
MINTiMINT-694680.

Structurei

Secondary structure

1235
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 56Combined sources17
Turni61 – 64Combined sources4
Helixi65 – 78Combined sources14
Turni79 – 82Combined sources4
Helixi85 – 88Combined sources4
Turni89 – 93Combined sources5
Turni96 – 98Combined sources3
Helixi100 – 103Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B2EX-ray3.00E/F/G/H21-104[»]
3SJAX-ray3.00C/D/G/H/J36-93[»]
3SJBX-ray3.30C/D19-103[»]
3SJCX-ray3.20C/D/G/H36-93[»]
3VLCX-ray4.50E21-104[»]
3ZS8X-ray3.00C/D21-104[»]
ProteinModelPortaliP53192.
SMRiP53192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili68 – 104UniRule annotationAdd BLAST37

Sequence similaritiesi

Belongs to the WRB/GET1 family.UniRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000000946.
InParanoidiP53192.
OMAiWTKNNRK.
OrthoDBiEOG092C5MDK.

Family and domain databases

HAMAPiMF_03113. Get1. 1 hit.
InterProiIPR027538. Get1_fungi.
IPR028945. WRB/Get1.
[Graphical view]
PfamiPF04420. CHD5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWAAAVAIF FIVVTKFLQY TNKYHEKWIS KFAPGNELSK KYLAKVKERH
60 70 80 90 100
ELKEFNNSIS AQDNYAKWTK NNRKLDSLDK EINNLKDEIQ SENKAFQAHL
110 120 130 140 150
HKLRLLALTV PFFVFKIMYG KTPVYKLSSS TSTLFPTFVS GVWSQGWLYV
160 170 180 190 200
LLHPLRTISQ KWHIMEGKFG ASKFDDMALQ SVSLGIWVWA LMNVINGVEF
210 220 230
IVKQLFLTPK MEAPASVETQ EEKALDAVDD AIILD
Length:235
Mass (Da):27,092
Last modified:October 1, 1996 - v1
Checksum:iC43DC5928D97DB7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72542 Genomic DNA. Translation: CAA96720.1.
BK006941 Genomic DNA. Translation: DAA08078.1.
PIRiS64022.
RefSeqiNP_011495.1. NM_001180885.1.

Genome annotation databases

EnsemblFungiiYGL020C; YGL020C; YGL020C.
GeneIDi852864.
KEGGisce:YGL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72542 Genomic DNA. Translation: CAA96720.1.
BK006941 Genomic DNA. Translation: DAA08078.1.
PIRiS64022.
RefSeqiNP_011495.1. NM_001180885.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B2EX-ray3.00E/F/G/H21-104[»]
3SJAX-ray3.00C/D/G/H/J36-93[»]
3SJBX-ray3.30C/D19-103[»]
3SJCX-ray3.20C/D/G/H36-93[»]
3VLCX-ray4.50E21-104[»]
3ZS8X-ray3.00C/D21-104[»]
ProteinModelPortaliP53192.
SMRiP53192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33226. 542 interactors.
DIPiDIP-6309N.
IntActiP53192. 3 interactors.
MINTiMINT-694680.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Proteomic databases

MaxQBiP53192.
PRIDEiP53192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL020C; YGL020C; YGL020C.
GeneIDi852864.
KEGGisce:YGL020C.

Organism-specific databases

EuPathDBiFungiDB:YGL020C.
SGDiS000002988. GET1.

Phylogenomic databases

HOGENOMiHOG000000946.
InParanoidiP53192.
OMAiWTKNNRK.
OrthoDBiEOG092C5MDK.

Enzyme and pathway databases

BioCyciYEAST:G3O-30540-MONOMER.

Miscellaneous databases

PROiP53192.

Family and domain databases

HAMAPiMF_03113. Get1. 1 hit.
InterProiIPR027538. Get1_fungi.
IPR028945. WRB/Get1.
[Graphical view]
PfamiPF04420. CHD5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGET1_YEAST
AccessioniPrimary (citable) accession number: P53192
Secondary accession number(s): D6VUB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.