Reviewed, UniProtKB/Swiss-Prot P53192 (GET1_YEAST)

Names and origin

Protein names

Recommended name:
    Golgi to ER traffic protein 1
Alternative name(s):
    Mitochondrial distribution and morphology protein 39

Gene names

Name:	GET1
Synonyms:	MDM39
Ordered Locus Names:	YGL020C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology. Ref.2 Ref.6
Subunit structure	Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Ref.6 Ref.4
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the WRB/GET1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Coiled coil Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular calcium ion homeostasis Inferred from mutant phenotype. Source: SGD mitochondrion organization Ref.2 Inferred from mutant phenotype. Source: SGD protein insertion into ER membrane Inferred from genetic interaction. Source: SGD response to unfolded protein Inferred from mutant phenotype. Source: SGD retrograde vesicle-mediated transport, Golgi to ER Ref.6 Inferred from direct assay. Source: SGD
Cellular component	GET complex Ref.6 Inferred from physical interaction. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay. Source: SGD
Molecular function	protein anchor Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GET2	P40056	1	EBI-23722,EBI-22604
GET3	Q12154	2	EBI-23722,EBI-2989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Golgi to ER traffic protein 1		PRO_0000096333
Regions
	Transmembrane	1 – 21	21	Potential
	Transmembrane	105 – 125	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	182 – 202	21	Potential
	Coiled coil	68 – 104	37	Potential
Amino acid modifications
	Glycosylation	56	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P53192-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C43DC5928D97DB7D	FASTA	235	27,092
	10 20 30 40 50 60 MHWAAAVAIF FIVVTKFLQY TNKYHEKWIS KFAPGNELSK KYLAKVKERH ELKEFNNSIS 70 80 90 100 110 120 AQDNYAKWTK NNRKLDSLDK EINNLKDEIQ SENKAFQAHL HKLRLLALTV PFFVFKIMYG 130 140 150 160 170 180 KTPVYKLSSS TSTLFPTFVS GVWSQGWLYV LLHPLRTISQ KWHIMEGKFG ASKFDDMALQ 190 200 210 220 230 SVSLGIWVWA LMNVINGVEF IVKQLFLTPK MEAPASVETQ EEKALDAVDD AIILD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae." Dimmer K.S., Fritz S., Fuchs F., Messerschmitt M., Weinbach N., Neupert W., Westermann B. Mol. Biol. Cell 13:847-853(2002) [PubMed: 11907266] [Abstract] Cited for: FUNCTION.
[3]	"Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry." Ho Y., Gruhler A., Heilbut A., Bader G.D., Moore L., Adams S.-L., Millar A., Taylor P., Bennett K., Boutilier K., Yang L., Wolting C., Donaldson I., Schandorff S., Shewnarane J., Vo M., Taggart J., Goudreault M. , Muskat B., Alfarano C., Dewar D., Lin Z., Michalickova K., Willems A.R., Sassi H., Nielsen P.A., Rasmussen K.J., Andersen J.R., Johansen L.E., Hansen L.H., Jespersen H., Podtelejnikov A., Nielsen E., Crawford J., Poulsen V., Soerensen B.D., Matthiesen J., Hendrickson R.C., Gleeson F., Pawson T., Moran M.F., Durocher D., Mann M., Hogue C.W.V., Figeys D., Tyers M. Nature 415:180-183(2002) [PubMed: 11805837] [Abstract] Cited for: IDENTIFICATION IN GET COMPLEX, MASS SPECTROMETRY.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile." Schuldiner M., Collins S.R., Thompson N.J., Denic V., Bhamidipati A., Punna T., Ihmels J., Andrews B., Boone C., Greenblatt J.F., Weissman J.S., Krogan N.J. Cell 123:507-519(2005) [PubMed: 16269340] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN GET COMPLEX, SUBCELLULAR LOCATION, INTERACTION WITH GET3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72542 Genomic DNA. Translation: CAA96720.1.
PIR	S64022.
RefSeq	NP_011495.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6309N.
IntAct	P53192. 4 interactions.
Proteomic databases
PeptideAtlas	P53192.
Genome annotation databases
Ensembl	YGL020C. Saccharomyces cerevisiae. [Contig view]
GeneID	852864.
GenomeReviews	Gene locus YGL020C in contig Y13135_GR.
KEGG	sce:YGL020C.
NMPDR	fig|4932.3.peg.2602.
Organism-specific databases
CYGD	YGL020c.
SGD	S000002988. GET1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53192.
OMA	P53192. ISAQDNY.
Gene expression databases
GermOnline	YGL020C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007514. CHD5. [Graphical view]
Pfam	PF04420. CHD5. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	972478.

Entry information

Entry name

GET1_YEAST

Accession

Primary (citable) accession number: P53192

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)