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Protein

Golgi to ER traffic protein 1

Gene

GET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in mitochondrial distribution and morphology.UniRule annotation5 Publications

GO - Molecular functioni

  • protein anchor Source: SGD

GO - Biological processi

  • protein insertion into ER membrane Source: SGD
  • retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  • tail-anchored membrane protein insertion into ER membrane Source: InterPro
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30540-MONOMER.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi to ER traffic protein 1UniRule annotation
Alternative name(s):
Guided entry of tail-anchored proteins 1UniRule annotation
Mitochondrial distribution and morphology protein 39
Gene namesi
Name:GET1UniRule annotation
Synonyms:MDM39
Ordered Locus Names:YGL020C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL020C.
SGDiS000002988. GET1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11LumenalUniRule annotation
Transmembranei2 – 2120HelicalUniRule annotationAdd
BLAST
Topological domaini22 – 10483CytoplasmicUniRule annotationAdd
BLAST
Transmembranei105 – 12521HelicalUniRule annotationAdd
BLAST
Topological domaini126 – 18156LumenalUniRule annotationAdd
BLAST
Transmembranei182 – 19817HelicalUniRule annotationAdd
BLAST
Topological domaini199 – 23537CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • GET complex Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Golgi to ER traffic protein 1PRO_0000414847Add
BLAST

Proteomic databases

MaxQBiP53192.
PeptideAtlasiP53192.

Interactioni

Subunit structurei

Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3.UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121543EBI-23722,EBI-2989

GO - Molecular functioni

  • protein anchor Source: SGD

Protein-protein interaction databases

BioGridi33226. 542 interactions.
DIPiDIP-6309N.
IntActiP53192. 3 interactions.
MINTiMINT-694680.

Structurei

Secondary structure

1
235
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 5617Combined sources
Turni61 – 644Combined sources
Helixi65 – 7814Combined sources
Turni79 – 824Combined sources
Helixi85 – 884Combined sources
Turni89 – 935Combined sources
Turni96 – 983Combined sources
Helixi100 – 1034Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B2EX-ray3.00E/F/G/H21-104[»]
3SJAX-ray3.00C/D/G/H/J36-93[»]
3SJBX-ray3.30C/D19-103[»]
3SJCX-ray3.20C/D/G/H36-93[»]
3VLCX-ray4.50E21-104[»]
3ZS8X-ray3.00C/D21-104[»]
ProteinModelPortaliP53192.
SMRiP53192. Positions 34-100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili68 – 10437UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the WRB/GET1 family.UniRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000000946.
InParanoidiP53192.
OMAiWSQGWLY.
OrthoDBiEOG7D5B1B.

Family and domain databases

HAMAPiMF_03113. Get1.
InterProiIPR027538. Get1_fungi.
IPR028945. WRB/Get1.
[Graphical view]
PfamiPF04420. CHD5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWAAAVAIF FIVVTKFLQY TNKYHEKWIS KFAPGNELSK KYLAKVKERH
60 70 80 90 100
ELKEFNNSIS AQDNYAKWTK NNRKLDSLDK EINNLKDEIQ SENKAFQAHL
110 120 130 140 150
HKLRLLALTV PFFVFKIMYG KTPVYKLSSS TSTLFPTFVS GVWSQGWLYV
160 170 180 190 200
LLHPLRTISQ KWHIMEGKFG ASKFDDMALQ SVSLGIWVWA LMNVINGVEF
210 220 230
IVKQLFLTPK MEAPASVETQ EEKALDAVDD AIILD
Length:235
Mass (Da):27,092
Last modified:October 1, 1996 - v1
Checksum:iC43DC5928D97DB7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72542 Genomic DNA. Translation: CAA96720.1.
BK006941 Genomic DNA. Translation: DAA08078.1.
PIRiS64022.
RefSeqiNP_011495.1. NM_001180885.1.

Genome annotation databases

EnsemblFungiiYGL020C; YGL020C; YGL020C.
GeneIDi852864.
KEGGisce:YGL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72542 Genomic DNA. Translation: CAA96720.1.
BK006941 Genomic DNA. Translation: DAA08078.1.
PIRiS64022.
RefSeqiNP_011495.1. NM_001180885.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B2EX-ray3.00E/F/G/H21-104[»]
3SJAX-ray3.00C/D/G/H/J36-93[»]
3SJBX-ray3.30C/D19-103[»]
3SJCX-ray3.20C/D/G/H36-93[»]
3VLCX-ray4.50E21-104[»]
3ZS8X-ray3.00C/D21-104[»]
ProteinModelPortaliP53192.
SMRiP53192. Positions 34-100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33226. 542 interactions.
DIPiDIP-6309N.
IntActiP53192. 3 interactions.
MINTiMINT-694680.

Protein family/group databases

TCDBi3.A.21.1.1. the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.

Proteomic databases

MaxQBiP53192.
PeptideAtlasiP53192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL020C; YGL020C; YGL020C.
GeneIDi852864.
KEGGisce:YGL020C.

Organism-specific databases

EuPathDBiFungiDB:YGL020C.
SGDiS000002988. GET1.

Phylogenomic databases

HOGENOMiHOG000000946.
InParanoidiP53192.
OMAiWSQGWLY.
OrthoDBiEOG7D5B1B.

Enzyme and pathway databases

BioCyciYEAST:G3O-30540-MONOMER.

Miscellaneous databases

PROiP53192.

Family and domain databases

HAMAPiMF_03113. Get1.
InterProiIPR027538. Get1_fungi.
IPR028945. WRB/Get1.
[Graphical view]
PfamiPF04420. CHD5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae."
    Dimmer K.S., Fritz S., Fuchs F., Messerschmitt M., Weinbach N., Neupert W., Westermann B.
    Mol. Biol. Cell 13:847-853(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: IDENTIFICATION IN GET COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile."
    Schuldiner M., Collins S.R., Thompson N.J., Denic V., Bhamidipati A., Punna T., Ihmels J., Andrews B., Boone C., Greenblatt J.F., Weissman J.S., Krogan N.J.
    Cell 123:507-519(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN GET COMPLEX, SUBCELLULAR LOCATION, INTERACTION WITH GET3.
  8. "Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae."
    Ando A., Suzuki C.
    Mol. Genet. Genomics 273:497-506(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae."
    Auld K.L., Hitchcock A.L., Doherty H.K., Frietze S., Huang L.S., Silver P.A.
    Genetics 174:215-227(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GET3.
  10. "The GET complex mediates insertion of tail-anchored proteins into the ER membrane."
    Schuldiner M., Metz J., Schmid V., Denic V., Rakwalska M., Schmitt H.D., Schwappach B., Weissman J.S.
    Cell 134:634-645(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The mechanism of tail-anchored protein insertion into the ER membrane."
    Wang F., Whynot A., Tung M., Denic V.
    Mol. Cell 43:738-750(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 21-104, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TOPOLOGY.
  12. "Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex."
    Stefer S., Reitz S., Wang F., Wild K., Pang Y.Y., Schwarz D., Bomke J., Hein C., Lohr F., Bernhard F., Denic V., Dotsch V., Sinning I.
    Science 333:758-762(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 19-103 IN COMPLEX WITH GET2 AND GET3, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.

Entry informationi

Entry nameiGET1_YEAST
AccessioniPrimary (citable) accession number: P53192
Secondary accession number(s): D6VUB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.