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Protein

Nicotinamidase

Gene

PNC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the deamidation of nicotinamide, an early step in the NAD+ salvage pathway. Positively regulates SIR2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide, an inhibitor of SIR2, during times of stress. Acts also on nicotinyl hydroxamate.3 Publications

Catalytic activityi

Nicotinamide + H2O = nicotinate + NH3.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide, HgCl2 and PCMB. Competitively inhibited by NAD, NMN and 3-acetylpyridine.2 Publications

Kineticsi

  1. KM=5.8 µM for nicotinamide3 Publications
  2. KM=32 µM for nicotinyl hydroxamate3 Publications
  3. KM=0.2 mM for pyrazinamide3 Publications
  1. Vmax=50.2 µmol/min/mg enzyme for nicotinamide3 Publications
  2. Vmax=59.4 µmol/min/mg enzyme for pyrazinamide3 Publications

pH dependencei

Optimum pH is 6.5-7.5.3 Publications

Temperature dependencei

Heating at 60 degrees Celsius inactivates the enzyme. Heating at 60 degrees Celsius in the presence of substrate prevents inactivation.3 Publications

Pathwayi: nicotinate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate from nicotinamide.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinamidase (PNC1)
This subpathway is part of the pathway nicotinate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate from nicotinamide, the pathway nicotinate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei8Sequence analysis1
Metal bindingi51ZincCombined sources1 Publication1
Metal bindingi53ZincCombined sources1 Publication1
Metal bindingi94ZincCombined sources1 Publication1
Active sitei122Sequence analysis1
Active sitei167NucleophileSequence analysis1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • nicotinamidase activity Source: SGD

GO - Biological processi

  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at telomere Source: SGD
  • NAD metabolic process Source: GO_Central
  • negative regulation of DNA amplification Source: SGD
  • nicotinamide metabolic process Source: GO_Central
  • nicotinate nucleotide salvage Source: SGD
  • replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YGL037C-MONOMER.
YEAST:YGL037C-MONOMER.
BRENDAi3.5.1.19. 984.
UniPathwayiUPA00830; UER00790.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamidase (EC:3.5.1.191 Publication)
Alternative name(s):
Nicotine deamidase
Short name:
NAMase
Gene namesi
Name:PNC1
Ordered Locus Names:YGL037C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL037C.
SGDiS000003005. PNC1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • cytoplasm Source: SGD
  • nucleus Source: SGD
  • peroxisome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002065581 – 216NicotinamidaseAdd BLAST216

Proteomic databases

MaxQBiP53184.
PRIDEiP53184.
TopDownProteomicsiP53184.

PTM databases

iPTMnetiP53184.

Expressioni

Inductioni

Induced during the stationary phase of growth, by calorie restriction, by various hyperosmotic shocks or by low-intensity stress (at protein level).2 Publications

Interactioni

Protein-protein interaction databases

BioGridi33210. 44 interactors.
DIPiDIP-2070N.
IntActiP53184. 10 interactors.
MINTiMINT-522829.

Chemistry databases

BindingDBiP53184.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 14Combined sources4
Helixi25 – 28Combined sources4
Helixi29 – 37Combined sources9
Helixi39 – 41Combined sources3
Beta strandi43 – 51Combined sources9
Helixi61 – 63Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi85 – 89Combined sources5
Helixi100 – 102Combined sources3
Helixi106 – 115Combined sources10
Beta strandi118 – 122Combined sources5
Beta strandi132 – 135Combined sources4
Helixi145 – 151Combined sources7
Beta strandi156 – 162Combined sources7
Turni164 – 166Combined sources3
Helixi167 – 177Combined sources11
Beta strandi181 – 190Combined sources10
Helixi196 – 208Combined sources13
Beta strandi212 – 214Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H0RX-ray2.90A/B/C/D/E/F/G1-216[»]
3V8EX-ray2.71A/B/C/D/E/F/G1-216[»]
ProteinModelPortaliP53184.
SMRiP53184.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53184.

Family & Domainsi

Sequence similaritiesi

Belongs to the PncA family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000064935.
HOGENOMiHOG000078666.
InParanoidiP53184.
KOiK01440.
OMAiPIHCVAN.
OrthoDBiEOG092C4BU3.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiIPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.

Sequencei

Sequence statusi: Complete.

P53184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLIVVDMQ NDFISPLGSL TVPKGEELIN PISDLMQDAD RDWHRIVVTR
60 70 80 90 100
DWHPSRHISF AKNHKDKEPY STYTYHSPRP GDDSTQEGIL WPVHCVKNTW
110 120 130 140 150
GSQLVDQIMD QVVTKHIKIV DKGFLTDREY YSAFHDIWNF HKTDMNKYLE
160 170 180 190 200
KHHTDEVYIV GVALEYCVKA TAISAAELGY KTTVLLDYTR PISDDPEVIN
210
KVKEELKAHN INVVDK
Length:216
Mass (Da):24,993
Last modified:October 1, 1996 - v1
Checksum:iC40B23C4228B6E3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72559 Genomic DNA. Translation: CAA96739.1.
AY558481 Genomic DNA. Translation: AAS56807.1.
BK006941 Genomic DNA. Translation: DAA08063.1.
PIRiS64039.
RefSeqiNP_011478.3. NM_001180902.3.

Genome annotation databases

EnsemblFungiiYGL037C; YGL037C; YGL037C.
GeneIDi852846.
KEGGisce:YGL037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72559 Genomic DNA. Translation: CAA96739.1.
AY558481 Genomic DNA. Translation: AAS56807.1.
BK006941 Genomic DNA. Translation: DAA08063.1.
PIRiS64039.
RefSeqiNP_011478.3. NM_001180902.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H0RX-ray2.90A/B/C/D/E/F/G1-216[»]
3V8EX-ray2.71A/B/C/D/E/F/G1-216[»]
ProteinModelPortaliP53184.
SMRiP53184.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33210. 44 interactors.
DIPiDIP-2070N.
IntActiP53184. 10 interactors.
MINTiMINT-522829.

Chemistry databases

BindingDBiP53184.

PTM databases

iPTMnetiP53184.

Proteomic databases

MaxQBiP53184.
PRIDEiP53184.
TopDownProteomicsiP53184.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL037C; YGL037C; YGL037C.
GeneIDi852846.
KEGGisce:YGL037C.

Organism-specific databases

EuPathDBiFungiDB:YGL037C.
SGDiS000003005. PNC1.

Phylogenomic databases

GeneTreeiENSGT00530000064935.
HOGENOMiHOG000078666.
InParanoidiP53184.
KOiK01440.
OMAiPIHCVAN.
OrthoDBiEOG092C4BU3.

Enzyme and pathway databases

UniPathwayiUPA00830; UER00790.
BioCyciMetaCyc:YGL037C-MONOMER.
YEAST:YGL037C-MONOMER.
BRENDAi3.5.1.19. 984.

Miscellaneous databases

EvolutionaryTraceiP53184.
PROiP53184.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
InterProiIPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNC1_YEAST
AccessioniPrimary (citable) accession number: P53184
Secondary accession number(s): D6VUA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Has a cis-peptide bond at 162-Val-Ala-163.
Present with 7720 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.