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P53179

- PALF_YEAST

UniProt

P53179 - PALF_YEAST

Protein

pH-response regulator protein palF/RIM8

Gene

RIM8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth.2 Publications

    GO - Biological processi

    1. invasive growth in response to glucose limitation Source: SGD
    2. meiotic nuclear division Source: SGD
    3. protein processing Source: SGD
    4. ubiquitin-dependent endocytosis Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30556-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    pH-response regulator protein palF/RIM8
    Alternative name(s):
    Regulator of IME2 protein 8
    Gene namesi
    Name:RIM8
    Synonyms:PAL3
    Ordered Locus Names:YGL045W
    ORF Names:YGL046W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL045w.
    SGDiS000003013. RIM8.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic side of plasma membrane Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 542542pH-response regulator protein palF/RIM8PRO_0000058194Add
    BLAST

    Proteomic databases

    MaxQBiP53179.
    PaxDbiP53179.

    Expressioni

    Inductioni

    Expressed under acidic conditions and repressed by the processed, active form of RIM101 under alkaline conditions.1 Publication

    Gene expression databases

    GenevestigatoriP53179.

    Interactioni

    Protein-protein interaction databases

    BioGridi33203. 248 interactions.
    DIPiDIP-5115N.
    MINTiMINT-528029.

    Structurei

    3D structure databases

    ProteinModelPortaliP53179.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi244 – 2507Poly-Ser

    Sequence similaritiesi

    Belongs to the arrestin family. PalF/RIM8 subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG255825.
    HOGENOMiHOG000115430.
    OMAiFRKDICQ.
    OrthoDBiEOG7HTHTN.

    Family and domain databases

    InterProiIPR011021. Arrestin-like_N.
    IPR011022. Arrestin_C-like.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF02752. Arrestin_C. 1 hit.
    PF00339. Arrestin_N. 1 hit.
    [Graphical view]
    SMARTiSM01017. Arrestin_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53179-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLLRLWNKE SRAPSKIKSH GIVGSYGNSM LAHNNVKQFR IDIDEPHRVW    50
    KPNESITGEA VIDIKRDITN VAIKLSLVCE VRVKTGNSPT SKNKRIEKTL 100
    EKSTFLYGQD YVKTAFSAKE KKPHVDKTTI LNGLSKGEHR FPFRIRIPRG 150
    RGMLSSIKFE RGSITYFLSC TLESLNNING LKKPEARCER EFAVIVPLDV 200
    SRLPKPKTKT VVLQSASMVQ NKKNKSTEDE SSSYTQLTQK STTSNSSSSS 250
    VNSKTSPLPN KTVTISVDIP QAGFMIGEII PIDVKIDHYK PFYAPAGLTT 300
    TLVRICRVGG AGKDDPMETF RKDICQSISP IYINPETLQF QSRVYLKVPL 350
    DAFSTLTTVG KFFSFQYYIE VMVNLSKKNV VYTESNRIIG TPIGEQNGLG 400
    VENNINRIQR KMLRMVNPET LENDSEGYES SIFFKDMVNV EKLKRLRNVT 450
    GMSIETVIGT TRSEQQQSDA SIPSQSSITA PQNSPSNLRD WLAPLNAYDS 500
    DDVPVPKYSP NDKVSVPSED KQELEQKRLQ QLESDPPPCD DY 542
    Length:542
    Mass (Da):60,864
    Last modified:June 21, 2005 - v2
    Checksum:i5639227A1225ACC2
    GO

    Sequence cautioni

    The sequence CAA96748.1 differs from that shown. Reason: Frameshift at positions 262, 270, 295 and 315.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ238650 Genomic DNA. Translation: CAB59334.1.
    Z72567 Genomic DNA. Translation: CAA96747.1. Frameshift.
    Z72568 Genomic DNA. Translation: CAA96748.1. Frameshift.
    AY693085 Genomic DNA. Translation: AAT93104.1.
    BK006941 Genomic DNA. Translation: DAA08055.1.
    PIRiS64049.
    S64050.
    RefSeqiNP_011470.4. NM_001180910.3.

    Genome annotation databases

    EnsemblFungiiYGL045W; YGL045W; YGL045W.
    GeneIDi852837.
    KEGGisce:YGL045W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ238650 Genomic DNA. Translation: CAB59334.1 .
    Z72567 Genomic DNA. Translation: CAA96747.1 . Frameshift.
    Z72568 Genomic DNA. Translation: CAA96748.1 . Frameshift.
    AY693085 Genomic DNA. Translation: AAT93104.1 .
    BK006941 Genomic DNA. Translation: DAA08055.1 .
    PIRi S64049.
    S64050.
    RefSeqi NP_011470.4. NM_001180910.3.

    3D structure databases

    ProteinModelPortali P53179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33203. 248 interactions.
    DIPi DIP-5115N.
    MINTi MINT-528029.

    Proteomic databases

    MaxQBi P53179.
    PaxDbi P53179.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL045W ; YGL045W ; YGL045W .
    GeneIDi 852837.
    KEGGi sce:YGL045W.

    Organism-specific databases

    CYGDi YGL045w.
    SGDi S000003013. RIM8.

    Phylogenomic databases

    eggNOGi NOG255825.
    HOGENOMi HOG000115430.
    OMAi FRKDICQ.
    OrthoDBi EOG7HTHTN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30556-MONOMER.

    Miscellaneous databases

    NextBioi 972411.

    Gene expression databases

    Genevestigatori P53179.

    Family and domain databases

    InterProi IPR011021. Arrestin-like_N.
    IPR011022. Arrestin_C-like.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF02752. Arrestin_C. 1 hit.
    PF00339. Arrestin_N. 1 hit.
    [Graphical view ]
    SMARTi SM01017. Arrestin_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and palH."
      Treton B., Blanchin-Roland S., Lambert M., Lepingle A., Gaillardin C.
      Mol. Gen. Genet. 263:505-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
      Feuermann M., de Montigny J., Potier S., Souciet J.-L.
      Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth."
      Li W., Mitchell A.P.
      Genetics 145:63-73(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae."
      Lamb T.M., Mitchell A.P.
      Mol. Cell. Biol. 23:677-686(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPALF_YEAST
    AccessioniPrimary (citable) accession number: P53179
    Secondary accession number(s): D6VU94, P53180, Q9URQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 784 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3