P53179 (PALF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
pH-response regulator protein palF/RIM8

Alternative name(s):
Regulator of IME2 protein 8

Gene names

Name:	RIM8
Synonyms:	PAL3
Ordered Locus Names:	YGL045W/YGL046W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Ref.1 Ref.6
Induction	Expressed under acidic conditions and repressed by the processed, active form of RIM101 under alkaline conditions. Ref.7
Miscellaneous	Present with 784 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the arrestin family. PalF/RIM8 subfamily.
Sequence caution	The sequence CAA96748.1 differs from that shown. Reason: Frameshift at positions 262, 270, 295 and 315.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	invasive growth in response to glucose limitation Inferred from mutant phenotype Ref.6. Source: SGD meiosis Inferred from mutant phenotype Ref.6. Source: SGD protein processing Inferred from mutant phenotype Ref.6. Source: SGD
Cellular component	internal side of plasma membrane Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	pH-response regulator protein palF/RIM8		PRO_0000058194
Regions
	Compositional bias	244 – 250	7	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	P53179 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 5639227A1225ACC2	FASTA	542	60,864
	10 20 30 40 50 60 MSLLRLWNKE SRAPSKIKSH GIVGSYGNSM LAHNNVKQFR IDIDEPHRVW KPNESITGEA 70 80 90 100 110 120 VIDIKRDITN VAIKLSLVCE VRVKTGNSPT SKNKRIEKTL EKSTFLYGQD YVKTAFSAKE 130 140 150 160 170 180 KKPHVDKTTI LNGLSKGEHR FPFRIRIPRG RGMLSSIKFE RGSITYFLSC TLESLNNING 190 200 210 220 230 240 LKKPEARCER EFAVIVPLDV SRLPKPKTKT VVLQSASMVQ NKKNKSTEDE SSSYTQLTQK 250 260 270 280 290 300 STTSNSSSSS VNSKTSPLPN KTVTISVDIP QAGFMIGEII PIDVKIDHYK PFYAPAGLTT 310 320 330 340 350 360 TLVRICRVGG AGKDDPMETF RKDICQSISP IYINPETLQF QSRVYLKVPL DAFSTLTTVG 370 380 390 400 410 420 KFFSFQYYIE VMVNLSKKNV VYTESNRIIG TPIGEQNGLG VENNINRIQR KMLRMVNPET 430 440 450 460 470 480 LENDSEGYES SIFFKDMVNV EKLKRLRNVT GMSIETVIGT TRSEQQQSDA SIPSQSSITA 490 500 510 520 530 540 PQNSPSNLRD WLAPLNAYDS DDVPVPKYSP NDKVSVPSED KQELEQKRLQ QLESDPPPCD DY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and palH." Treton B., Blanchin-Roland S., Lambert M., Lepingle A., Gaillardin C. Mol. Gen. Genet. 263:505-513(2000) [PubMed: 10821185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes." Feuermann M., de Montigny J., Potier S., Souciet J.-L. Yeast 13:861-869(1997) [PubMed: 9234674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth." Li W., Mitchell A.P. Genetics 145:63-73(1997) [PubMed: 9017390] [Abstract] Cited for: FUNCTION.
[7]	"The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae." Lamb T.M., Mitchell A.P. Mol. Cell. Biol. 23:677-686(2003) [PubMed: 12509465] [Abstract] Cited for: INDUCTION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ238650 Genomic DNA. Translation: CAB59334.1. Z72567 Genomic DNA. Translation: CAA96747.1. Frameshift. Z72568 Genomic DNA. Translation: CAA96748.1. Frameshift. AY693085 Genomic DNA. Translation: AAT93104.1. BK006941 Genomic DNA. Translation: DAA08055.1.
PIR	S64049. S64050.
RefSeq	NP_011470.2. NM_001180910.1.
3D structure databases
ProteinModelPortal	P53179.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5115N.
MINT	MINT-528029.
STRING	P53179.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL045W; YGL045W; YGL045W.
GeneID	852837.
KEGG	sce:YGL045W.
NMPDR	fig|4932.3.peg.2575.
Organism-specific databases
CYGD	YGL045w.
SGD	S000003013. RIM8.
Phylogenomic databases
eggNOG	fuNOG08101.
GeneTree	EFGT00050000003158.
HOGENOM	HBG204313.
OMA	FRKDICQ.
OrthoDB	EOG4HQHTJ.
Gene expression databases
ArrayExpress	P53179.
Genevestigator	P53179.
GermOnline	YGL045W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011021. Arrestin-like_N. IPR011022. Arrestin_C-like. IPR014756. Ig_E-set. [Graphical view]
Pfam	PF02752. Arrestin_C. 1 hit. PF00339. Arrestin_N. 1 hit. [Graphical view]
SMART	SM01017. Arrestin_C. 1 hit. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
NextBio	972411.

Entry information

Entry name

PALF_YEAST

Accession

Primary (citable) accession number: P53179
Secondary accession number(s): D6VU94, P53180, Q9URQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program