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Protein

pH-response regulator protein palF/RIM8

Gene

RIM8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth.2 Publications

GO - Biological processi

  1. invasive growth in response to glucose limitation Source: SGD
  2. meiotic nuclear division Source: SGD
  3. protein processing Source: SGD
  4. ubiquitin-dependent endocytosis Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-30556-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
pH-response regulator protein palF/RIM8
Alternative name(s):
Regulator of IME2 protein 8
Gene namesi
Name:RIM8
Synonyms:PAL3
Ordered Locus Names:YGL045W
ORF Names:YGL046W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL045w.
SGDiS000003013. RIM8.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic side of plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542pH-response regulator protein palF/RIM8PRO_0000058194Add
BLAST

Proteomic databases

MaxQBiP53179.
PaxDbiP53179.

Expressioni

Inductioni

Expressed under acidic conditions and repressed by the processed, active form of RIM101 under alkaline conditions.1 Publication

Gene expression databases

GenevestigatoriP53179.

Interactioni

Protein-protein interaction databases

BioGridi33203. 248 interactions.
DIPiDIP-5115N.
MINTiMINT-528029.

Structurei

3D structure databases

ProteinModelPortaliP53179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi244 – 2507Poly-Ser

Sequence similaritiesi

Belongs to the arrestin family. PalF/RIM8 subfamily.Curated

Phylogenomic databases

eggNOGiNOG255825.
HOGENOMiHOG000115430.
InParanoidiP53179.
OMAiFRKDICQ.
OrthoDBiEOG7HTHTN.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

P53179-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLLRLWNKE SRAPSKIKSH GIVGSYGNSM LAHNNVKQFR IDIDEPHRVW
60 70 80 90 100
KPNESITGEA VIDIKRDITN VAIKLSLVCE VRVKTGNSPT SKNKRIEKTL
110 120 130 140 150
EKSTFLYGQD YVKTAFSAKE KKPHVDKTTI LNGLSKGEHR FPFRIRIPRG
160 170 180 190 200
RGMLSSIKFE RGSITYFLSC TLESLNNING LKKPEARCER EFAVIVPLDV
210 220 230 240 250
SRLPKPKTKT VVLQSASMVQ NKKNKSTEDE SSSYTQLTQK STTSNSSSSS
260 270 280 290 300
VNSKTSPLPN KTVTISVDIP QAGFMIGEII PIDVKIDHYK PFYAPAGLTT
310 320 330 340 350
TLVRICRVGG AGKDDPMETF RKDICQSISP IYINPETLQF QSRVYLKVPL
360 370 380 390 400
DAFSTLTTVG KFFSFQYYIE VMVNLSKKNV VYTESNRIIG TPIGEQNGLG
410 420 430 440 450
VENNINRIQR KMLRMVNPET LENDSEGYES SIFFKDMVNV EKLKRLRNVT
460 470 480 490 500
GMSIETVIGT TRSEQQQSDA SIPSQSSITA PQNSPSNLRD WLAPLNAYDS
510 520 530 540
DDVPVPKYSP NDKVSVPSED KQELEQKRLQ QLESDPPPCD DY
Length:542
Mass (Da):60,864
Last modified:June 21, 2005 - v2
Checksum:i5639227A1225ACC2
GO

Sequence cautioni

The sequence CAA96748.1 differs from that shown. Reason: Frameshift at positions 262, 270, 295 and 315. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238650 Genomic DNA. Translation: CAB59334.1.
Z72567 Genomic DNA. Translation: CAA96747.1. Frameshift.
Z72568 Genomic DNA. Translation: CAA96748.1. Frameshift.
AY693085 Genomic DNA. Translation: AAT93104.1.
BK006941 Genomic DNA. Translation: DAA08055.1.
PIRiS64049.
S64050.
RefSeqiNP_011470.4. NM_001180910.3.

Genome annotation databases

EnsemblFungiiYGL045W; YGL045W; YGL045W.
GeneIDi852837.
KEGGisce:YGL045W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238650 Genomic DNA. Translation: CAB59334.1.
Z72567 Genomic DNA. Translation: CAA96747.1. Frameshift.
Z72568 Genomic DNA. Translation: CAA96748.1. Frameshift.
AY693085 Genomic DNA. Translation: AAT93104.1.
BK006941 Genomic DNA. Translation: DAA08055.1.
PIRiS64049.
S64050.
RefSeqiNP_011470.4. NM_001180910.3.

3D structure databases

ProteinModelPortaliP53179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33203. 248 interactions.
DIPiDIP-5115N.
MINTiMINT-528029.

Proteomic databases

MaxQBiP53179.
PaxDbiP53179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL045W; YGL045W; YGL045W.
GeneIDi852837.
KEGGisce:YGL045W.

Organism-specific databases

CYGDiYGL045w.
SGDiS000003013. RIM8.

Phylogenomic databases

eggNOGiNOG255825.
HOGENOMiHOG000115430.
InParanoidiP53179.
OMAiFRKDICQ.
OrthoDBiEOG7HTHTN.

Enzyme and pathway databases

BioCyciYEAST:G3O-30556-MONOMER.

Miscellaneous databases

NextBioi972411.

Gene expression databases

GenevestigatoriP53179.

Family and domain databases

InterProiIPR011021. Arrestin-like_N.
IPR011022. Arrestin_C-like.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF02752. Arrestin_C. 1 hit.
PF00339. Arrestin_N. 1 hit.
[Graphical view]
SMARTiSM01017. Arrestin_C. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ambient pH signalling in ascomycetous yeasts involves homologues of the Aspergillus nidulans genes palF and palH."
    Treton B., Blanchin-Roland S., Lambert M., Lepingle A., Gaillardin C.
    Mol. Gen. Genet. 263:505-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Proteolytic activation of Rim1p, a positive regulator of yeast sporulation and invasive growth."
    Li W., Mitchell A.P.
    Genetics 145:63-73(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae."
    Lamb T.M., Mitchell A.P.
    Mol. Cell. Biol. 23:677-686(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPALF_YEAST
AccessioniPrimary (citable) accession number: P53179
Secondary accession number(s): D6VU94, P53180, Q9URQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 21, 2005
Last modified: January 7, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.