UDP-N-acetylglucosamine transferase subunit ALG13 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P53178 (ALG13_YEAST)

Last modified January 19, 2010. Version 69. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine transferase subunit ALG13
    EC=2.4.1.141
Alternative name(s):
    Asparagine-linked glycosylation protein 13

Gene names

Name:	ALG13
Ordered Locus Names:	YGL047W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Ref.5 Ref.6
Catalytic activity	UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.
Subunit structure	Heterodimer with ALG14 to form a functional enzyme.
Subcellular location	Endoplasmic reticulum Ref.6 Ref.3.
Miscellaneous	Present with 1950 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 28 family.

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Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	dolichol-linked oligosaccharide biosynthetic process Ref.5 Inferred from direct assay. Source: SGD lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	UDP-N-acetylglucosamine transferase complex Ref.6 Inferred from physical interaction. Source: SGD cytosol Inferred from direct assay. Source: SGD extrinsic to endoplasmic reticulum membrane Ref.6 Inferred from direct assay. Source: SGD membrane fraction Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity Ref.5 Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ALG14	P38242	1	EBI-23770,EBI-21477

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 202

202

UDP-N-acetylglucosamine transferase subunit ALG13

PRO_0000215605

Secondary structure

202

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P53178-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 9D5CDB366BBC1ED0
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FASTA

202

22,661

        10         20         30         40         50         60 
MGIIEEKALF VTCGATVPFP KLVSCVLSDE FCQELIQYGF VRLIIQFGRN YSSEFEHLVQ 

        70         80         90        100        110        120 
ERGGQRESQK IPIDQFGCGD TARQYVLMNG KLKVIGFDFS TKMQSIIRDY SDLVISHAGT 

       130        140        150        160        170        180 
GSILDSLRLN KPLIVCVNDS LMDNHQQQIA DKFVELGYVW SCAPTETGLI AGLRASQTEK 

       190        200 
LKPFPVSHNP SFERLLVETI YS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes." Feuermann M., de Montigny J., Potier S., Souciet J.-L. Yeast 13:861-869(1997) [PubMed: 9234674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Two proteins homologous to the N- and C-terminal domains of the bacterial glycosyltransferase Murg are required for the second step of dolichyl-linked oligosaccharide synthesis in Saccharomyces cerevisiae." Chantret I., Dancourt J., Barbat A., Moore S.E.H. J. Biol. Chem. 280:9236-9242(2005) [PubMed: 15615718] [Abstract] Cited for: FUNCTION.
[6]	"Alg14 recruits Alg13 to the cytoplasmic face of the endoplasmic reticulum to form a novel bipartite UDP-N-acetylglucosamine transferase required for the second step of N-linked glycosylation." Gao X.-D., Tachikawa H., Sato T., Jigami Y., Dean N. J. Biol. Chem. 280:36254-36262(2005) [PubMed: 16100110] [Abstract] Cited for: FUNCTION, INTERACTION WITH ALG14, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z72569 Genomic DNA. Translation: CAA96749.1.

PIR

S64051.

RefSeq

NP_011468.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JZC	NMR	-	A	2-202	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4726N.

IntAct

P53178. 3 interactions.

STRING

P53178.

Protein family/group databases

CAZy

GT1. Glycosyltransferase Family 1.

Genome annotation databases

Ensembl

YGL047W; YGL047W; YGL047W; Saccharomyces cerevisiae. [Genome view]

GeneID

852835.

KEGG

sce:YGL047W.

NMPDR

fig|4932.3.peg.2574.

Organism-specific databases

CYGD

YGL047w.

SGD

S000003015. ALG13.

Phylogenomic databases

eggNOG

HOGENOM

OMA

OrthoDB

PhylomeDB

P53178.

Enzyme and pathway databases

BRENDA

2.4.1.141. 250.

Gene expression databases

ArrayExpress

P53178.

Genevestigator

P53178.

GermOnline

YGL047W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007235. Glyco_trans_28_C.
[Graphical view]

Pfam

PF04101. Glyco_tran_28_C. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

972406.

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Entry information

Entry name

ALG13_YEAST

Accession

Primary (citable) accession number: P53178

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents