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Protein

tRNA wybutosine-synthesizing protein 3

Gene

TYW3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.1 Publication

Miscellaneous

Present with 3390 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4-demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-(3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe).1 Publication

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

GO - Molecular functioni

  • tRNA methyltransferase activity Source: SGD

GO - Biological processi

  • tRNA methylation Source: SGD
  • tRNA modification Source: Reactome
  • wybutosine biosynthetic process Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30560-MONOMER
YEAST:G3O-30560-MONOMER
BRENDAi2.1.1.282 984
UniPathwayiUPA00375

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 3 (EC:2.1.1.282)
Short name:
tRNA-yW-synthesizing protein 3
Alternative name(s):
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
Gene namesi
Name:TYW3
Ordered Locus Names:YGL050W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL050W
SGDiS000003018 TYW3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002027691 – 273tRNA wybutosine-synthesizing protein 3Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineCombined sources1
Modified residuei238PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53177
PaxDbiP53177
PRIDEiP53177

PTM databases

iPTMnetiP53177

Interactioni

Protein-protein interaction databases

BioGridi33198, 94 interactors
DIPiDIP-2849N
IntActiP53177, 4 interactors
MINTiP53177
STRINGi4932.YGL050W

Structurei

3D structure databases

ProteinModelPortaliP53177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TYW3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018149
HOGENOMiHOG000247848
InParanoidiP53177
KOiK15450
OMAiLHVKCRD
OrthoDBiEOG092C5EC9

Family and domain databases

Gene3Di3.30.1960.10, 1 hit
InterProiView protein in InterPro
IPR003827 tRNA_yW-synthesising
IPR036602 tRNA_yW-synthesising-like_sf
PfamiView protein in Pfam
PF02676 TYW3, 1 hit
SUPFAMiSSF111278 SSF111278, 1 hit

Sequencei

Sequence statusi: Complete.

P53177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQNAFEQK KRAILNEIDS TQPDLSPKGT IDELCLPIID LINASADMVT
60 70 80 90 100
TSSCSGRVSV FLEGTKSYNG EVKIGGKGQG GKWLYVTHDR EKVIGWLDEL
110 120 130 140 150
KSKSEFSFEL SGKEIPTEKV TGSIRYILYK YEPFILHVKC RDFQAASKLY
160 170 180 190 200
NTAMSCGFRE SGIGSNNLVA IRINIKLDVP LGYLDETSGT LKFFVTPEYV
210 220 230 240 250
SVLDSLSLSK FDENTRKMQA LYDRIEKELI NCAPDVNSKV NITPIETKEE
260 270
RRERKKREGM ERQRQLKSPQ NVL
Length:273
Mass (Da):30,806
Last modified:October 1, 1996 - v1
Checksum:iA36D61F625B0D8EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72572 Genomic DNA Translation: CAA96752.1
BK006941 Genomic DNA Translation: DAA08051.1
PIRiS64054
RefSeqiNP_011465.3, NM_001180915.3

Genome annotation databases

EnsemblFungiiYGL050W; YGL050W; YGL050W
GeneIDi852832
KEGGisce:YGL050W

Similar proteinsi

Entry informationi

Entry nameiTYW3_YEAST
AccessioniPrimary (citable) accession number: P53177
Secondary accession number(s): D6VU90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health