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Protein

tRNA wybutosine-synthesizing protein 3

Gene

TYW3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4-demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-(3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe).1 Publication

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

GO - Molecular functioni

  • tRNA methyltransferase activity Source: SGD

GO - Biological processi

  • tRNA methylation Source: SGD
  • tRNA modification Source: Reactome
  • wybutosine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30560-MONOMER.
YEAST:G3O-30560-MONOMER.
BRENDAi2.1.1.282. 984.
ReactomeiR-SCE-6782861. Synthesis of wybutosine at G37 of tRNA(Phe).
UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 3 (EC:2.1.1.282)
Short name:
tRNA-yW-synthesizing protein 3
Alternative name(s):
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
Gene namesi
Name:TYW3
Ordered Locus Names:YGL050W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL050W.
SGDiS000003018. TYW3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273tRNA wybutosine-synthesizing protein 3PRO_0000202769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261PhosphoserineCombined sources
Modified residuei238 – 2381PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53177.

PTM databases

iPTMnetiP53177.

Interactioni

Protein-protein interaction databases

BioGridi33198. 13 interactions.
DIPiDIP-2849N.
IntActiP53177. 1 interaction.
MINTiMINT-1722148.

Structurei

3D structure databases

ProteinModelPortaliP53177.
SMRiP53177. Positions 38-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TYW3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018149.
HOGENOMiHOG000247848.
InParanoidiP53177.
KOiK15450.
OMAiPMILHIM.
OrthoDBiEOG73V6WR.

Family and domain databases

Gene3Di3.30.1960.10. 1 hit.
InterProiIPR003827. tRNA_yW-synthesising.
[Graphical view]
PfamiPF02676. TYW3. 1 hit.
[Graphical view]
SUPFAMiSSF111278. SSF111278. 1 hit.

Sequencei

Sequence statusi: Complete.

P53177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQNAFEQK KRAILNEIDS TQPDLSPKGT IDELCLPIID LINASADMVT
60 70 80 90 100
TSSCSGRVSV FLEGTKSYNG EVKIGGKGQG GKWLYVTHDR EKVIGWLDEL
110 120 130 140 150
KSKSEFSFEL SGKEIPTEKV TGSIRYILYK YEPFILHVKC RDFQAASKLY
160 170 180 190 200
NTAMSCGFRE SGIGSNNLVA IRINIKLDVP LGYLDETSGT LKFFVTPEYV
210 220 230 240 250
SVLDSLSLSK FDENTRKMQA LYDRIEKELI NCAPDVNSKV NITPIETKEE
260 270
RRERKKREGM ERQRQLKSPQ NVL
Length:273
Mass (Da):30,806
Last modified:October 1, 1996 - v1
Checksum:iA36D61F625B0D8EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72572 Genomic DNA. Translation: CAA96752.1.
BK006941 Genomic DNA. Translation: DAA08051.1.
PIRiS64054.
RefSeqiNP_011465.3. NM_001180915.3.

Genome annotation databases

EnsemblFungiiYGL050W; YGL050W; YGL050W.
GeneIDi852832.
KEGGisce:YGL050W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72572 Genomic DNA. Translation: CAA96752.1.
BK006941 Genomic DNA. Translation: DAA08051.1.
PIRiS64054.
RefSeqiNP_011465.3. NM_001180915.3.

3D structure databases

ProteinModelPortaliP53177.
SMRiP53177. Positions 38-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33198. 13 interactions.
DIPiDIP-2849N.
IntActiP53177. 1 interaction.
MINTiMINT-1722148.

PTM databases

iPTMnetiP53177.

Proteomic databases

MaxQBiP53177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL050W; YGL050W; YGL050W.
GeneIDi852832.
KEGGisce:YGL050W.

Organism-specific databases

EuPathDBiFungiDB:YGL050W.
SGDiS000003018. TYW3.

Phylogenomic databases

GeneTreeiENSGT00390000018149.
HOGENOMiHOG000247848.
InParanoidiP53177.
KOiK15450.
OMAiPMILHIM.
OrthoDBiEOG73V6WR.

Enzyme and pathway databases

UniPathwayiUPA00375.
BioCyciMetaCyc:G3O-30560-MONOMER.
YEAST:G3O-30560-MONOMER.
BRENDAi2.1.1.282. 984.
ReactomeiR-SCE-6782861. Synthesis of wybutosine at G37 of tRNA(Phe).

Miscellaneous databases

PROiP53177.

Family and domain databases

Gene3Di3.30.1960.10. 1 hit.
InterProiIPR003827. tRNA_yW-synthesising.
[Graphical view]
PfamiPF02676. TYW3. 1 hit.
[Graphical view]
SUPFAMiSSF111278. SSF111278. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The characterization of two new clusters of duplicated genes suggests a 'Lego' organization of the yeast Saccharomyces cerevisiae chromosomes."
    Feuermann M., de Montigny J., Potier S., Souciet J.-L.
    Yeast 13:861-869(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA."
    Noma A., Kirino Y., Ikeuchi Y., Suzuki T.
    EMBO J. 25:2142-2154(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTYW3_YEAST
AccessioniPrimary (citable) accession number: P53177
Secondary accession number(s): D6VU90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3390 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.